Restriction map for
pFN21AE4121
(Circular) (Six-base) MAPSORT of: pFN21AE4121.seq Check: 4991 from: 1 to: 6220
pFN21AE4121 6220 bp
With 182 enzymes: SgfI *
March 28, 2012 13:17 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4030 278
Size: 53 83 186 936 2494 2468
Fragments arranged by size:
2494 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3563 1937
Size: 1626 4594
Acc65I G'GTAC_C
Cuts at: 3541 3541
Size: 6220
AclI AA'CG_TT
Cuts at: 1563 3468 4347 4720 1563
Size: 1905 879 373 3063
Fragments arranged by size:
3063 1905 879 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2344 3023 4283 5667 5935 1632
Size: 712 679 1260 1384 268 1917
Fragments arranged by size:
1917 1384 1260 712 679 268
AflII C'TTAA_G
Cuts at: 820 1017 2054 820
Size: 197 1037 4986
Fragments arranged by size:
4986 1037 197
AflIII A'CryG_T
Cuts at: 1203 3198 5119 6096 1203
Size: 1995 1921 977 1327
Fragments arranged by size:
1995 1921 1327 977
AgeI A'CCGG_T
Cuts at: 5051 5051
Size: 6220
AhdI GACnn_n'nnGTC
Cuts at: 3160 4949 3160
Size: 1789 4431
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6220
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6220
AlwNI CAG_nnn'CTG
Cuts at: 2803 3963 5535 2803
Size: 1160 1572 3488
Fragments arranged by size:
3488 1572 1160
ApaI G_GGCC'C
Cuts at: 2281 2281
Size: 6220
ApaLI G'TGCA_C
Cuts at: 4277 5433 4277
Size: 1156 5064
ApoI r'AATT_y
Cuts at: 1493 2073 2354 2394 2477 3529 3755 3907 6016
Size: 580 281 40 83 1052 226 152 2109
Cuts at: 6016 1493
Size: 1697
Fragments arranged by size:
2109 1697 1052 580 281 226 152 83
40
AseI AT'TA_AT
Cuts at: 160 1032 4774 160
Size: 872 3742 1606
Fragments arranged by size:
3742 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6220
AvaI C'yCGr_G
Cuts at: 1870 1943 2898 3545 1870
Size: 73 955 647 4545
Fragments arranged by size:
4545 955 647 73
BamHI G'GATC_C
Cuts at: 3550 3917 3550
Size: 367 5853
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3541 4996 618
Size: 325 217 627 1754 1455 1842
Fragments arranged by size:
1842 1754 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2281 2779 3539 1391
Size: 890 498 760 4072
Fragments arranged by size:
4072 890 760 498
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2007 2333 2829 928
Size: 971 108 326 496 4319
Fragments arranged by size:
4319 971 496 326 108
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4411 4445 1903
Size: 34 2474 34 3678
Fragments arranged by size:
3678 2474 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4112 5322 4112
Size: 1210 5010
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6220
BfrBI ATG'CAT
Cuts at: 2548 2548
Size: 6220
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 4831 136
Size: 107 122 71 631 605 337 2822 1525
Fragments arranged by size:
2822 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 2749 6215 2749
Size: 3466 2754
BlpI GC'TnA_GC
Cuts at: 3637 3637
Size: 6220
Bme1580I G_kGCm'C
Cuts at: 1790 2281 4281 5437 1790
Size: 491 2000 1156 2573
Fragments arranged by size:
2573 2000 1156 491
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6220
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2218 2409 3154
Size: 416 53 83 399 24 801 191 745
Cuts at: 3154 3951 4909 442
Size: 797 958 1753
Fragments arranged by size:
1753 958 801 797 745 416 399 191
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6220
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3261 3261
Size: 6220
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2182 2213 2631 3114 4880 1239
Size: 943 31 418 483 1766 2579
Fragments arranged by size:
2579 1766 943 483 418 31
Bpu10I CC'TnA_GC
Cuts at: 2592 3259 2592
Size: 667 5553
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2468 3489 4345 5210 5508 2468
Size: 1021 856 865 298 3180
Fragments arranged by size:
3180 1021 865 856 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3249 4883 882
Size: 617 1750 1634 2219
Fragments arranged by size:
2219 1750 1634 617
BsaAI yAC'GTr
Cuts at: 493 1204 4011 493
Size: 711 2807 2702
Fragments arranged by size:
2807 2702 711
BsaBI GATnn'nnATC
Cuts at: 3189 6214 3189
Size: 3025 3195
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2012 2975 3156 3264
Size: 53 83 186 936 479 963 181 108
Cuts at: 3264 4027 4409 5044 275
Size: 763 382 635 1451
Fragments arranged by size:
1451 963 936 763 635 479 382 186
181 108 83 53
BsaWI w'CCGG_w
Cuts at: 4653 5051 5325 5472 5883 4653
Size: 398 274 147 411 4990
Fragments arranged by size:
4990 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5995
Fragments arranged by size:
5995 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6220
BseYI C'CCAG_C
Cuts at: 2784 5423 5919 2784
Size: 2639 496 3085
Fragments arranged by size:
3085 2639 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2947 1163
Size: 573 1211 4436
Fragments arranged by size:
4436 1211 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3670 4431 5459 1540
Size: 275 186 1669 761 1028 2301
Fragments arranged by size:
2301 1669 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2593 4281 4366 5437 2593
Size: 1688 85 1071 3376
Fragments arranged by size:
3376 1688 1071 85
BsmI GAATG_Cn'
Cuts at: 2550 3731 3824 2550
Size: 1181 93 4946
Fragments arranged by size:
4946 1181 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2705 2705
Size: 6220
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2281 2593 2779 3539 4281 4366 5437
Size: 399 491 312 186 760 742 85 1071
Cuts at: 5437 1391
Size: 2174
Fragments arranged by size:
2174 1071 760 742 491 399 312 186
85
BspEI T'CCGG_A
Cuts at: 5883 5883
Size: 6220
BspHI T'CATG_A
Cuts at: 2691 3327 4109 5839 2691
Size: 636 782 1730 3072
Fragments arranged by size:
3072 1730 782 636
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3575 844
Size: 2731 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4107 1394
Size: 429 2284 3507
Fragments arranged by size:
3507 2284 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4715 4889 65
Size: 1183 3467 174 1396
Fragments arranged by size:
3467 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2430 2971 4864 5051 1700
Size: 110 620 541 1893 187 2869
Fragments arranged by size:
2869 1893 620 541 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6220
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6220
BssSI C'ACGA_G
Cuts at: 2631 4280 5292 2631
Size: 1649 1012 3559
Fragments arranged by size:
3559 1649 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6220
BstBI TT'CG_AA
Cuts at: 5024 5024
Size: 6220
BstEII G'GTnAC_C
Cuts at: 1184 2338 1184
Size: 1154 5066
BstXI CCAn_nnnn'nTGG
Cuts at: 3346 3346
Size: 6220
BstYI r'GATC_y
Cuts at: 1972 2092 2560 2749 3289 3550 3917 4302 4319
Size: 120 468 189 540 261 367 385 17
Cuts at: 4319 5771 5857 5869 6215 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 540 468 385 367 346 261
189 120 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5668
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5481
Fragments arranged by size:
5481 683 56
BtsI GCAGTG_nn'
Cuts at: 3494 3736 4530 4550 3494
Size: 242 794 20 5164
Fragments arranged by size:
5164 794 242 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6220
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6185
DraI TTT'AAA
Cuts at: 3524 3524
Size: 6220
DraIII CAC_nnn'GTG
Cuts at: 2611 2925 2611
Size: 314 5906
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5227 809
Size: 507 3911 1802
Fragments arranged by size:
3911 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2613 3205 3667
Size: 54 1425 50 275 18 783 592 462
Cuts at: 3667 4556 6185 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 783 592 462 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3667 1537
Size: 275 1855 4090
Fragments arranged by size:
4090 1855 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2163 2238 4150 1353
Size: 810 75 1912 3423
Fragments arranged by size:
3423 1912 810 75
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2234 4807 5181 5327 387
Size: 1064 138 645 2573 374 146 1280
Fragments arranged by size:
2573 1280 1064 645 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2182 2213 2344 2631 3023 3114 4283
Size: 393 550 31 131 287 392 91 1169
Cuts at: 4283 4880 5667 5935 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1169 787 597 550 393 392 287
268 131 91 31
EcoNI CCTnn'n_nnAGG
Cuts at: 3217 3217
Size: 6220
EcoO109I rG'GnC_Cy
Cuts at: 2277 2277
Size: 6220
EcoRI G'AATT_C
Cuts at: 2073 3529 3907 2073
Size: 1456 378 4386
Fragments arranged by size:
4386 1456 378
EcoRV GAT'ATC
Cuts at: 2869 2869
Size: 6220
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2374 2956 2374
Size: 582 5638
FspI TGC'GCA
Cuts at: 4726 4726
Size: 6220
HaeII r_GCGC'y
Cuts at: 1678 5367 1678
Size: 3689 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2444 2476 2614 2646 2656
Size: 32 135 32 872 32 138 32 10
Cuts at: 2656 2688 2960 2992 3146 3178 3227 3259 3281
Size: 32 272 32 154 32 49 32 22
Cuts at: 3281 3313 4861 4893 4935 4967 1373
Size: 32 1548 32 42 32 2626
Fragments arranged by size:
2626 1548 872 272 154 138 135 49
42 32 32 32 32 32 32 32
32 32 32 32 22 10
HincII GTy'rAC
Cuts at: 669 1657 1938 3564 3810 669
Size: 988 281 1626 246 3079
Fragments arranged by size:
3079 1626 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6220
HpaI GTT'AAC
Cuts at: 3810 3810
Size: 6220
KpnI G_GTAC'C
Cuts at: 3545 3545
Size: 6220
MfeI C'AATT_G
Cuts at: 3819 3819
Size: 6220
MluI A'CGCG_T
Cuts at: 6096 6096
Size: 6220
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2359 5334 5518 1305
Size: 1054 2975 184 2007
Fragments arranged by size:
2975 2007 1054 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3207 10
Size: 54 1425 343 1375 3023
Fragments arranged by size:
3023 1425 1375 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2876 4178 4537 4696 518
Size: 648 1710 1302 359 159 2042
Fragments arranged by size:
2042 1710 1302 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2233 2981 3636 4313 5461 5706 1606
Size: 627 748 655 677 1148 245 2120
Fragments arranged by size:
2120 1148 748 677 655 627 245
NaeI GCC'GGC
Cuts at: 2973 2973
Size: 6220
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5668
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6220
NgoMIV G'CCGG_C
Cuts at: 2971 2971
Size: 6220
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6220
NotI GC'GGCC_GC
Cuts at: 3667 3667
Size: 6220
NsiI A_TGCA'T
Cuts at: 2550 2550
Size: 6220
NspI r_CATG'y
Cuts at: 1132 1229 3202 3578 5123 1132
Size: 97 1973 376 1545 2229
Fragments arranged by size:
2229 1973 1545 376 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6220
PciI A'CATG_T
Cuts at: 3198 5119 3198
Size: 1921 4299
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2029 2160 2611 1108
Size: 921 131 451 4717
Fragments arranged by size:
4717 921 451 131
PmeI GTTT'AAAC
Cuts at: 3524 3524
Size: 6220
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4289 4321 5802 5834 4289
Size: 32 1481 32 4675
Fragments arranged by size:
4675 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6220
PsiI TTA'TAA
Cuts at: 3790 3790
Size: 6220
PspOMI G'GGCC_C
Cuts at: 2277 2277
Size: 6220
PstI C_TGCA'G
Cuts at: 830 3572 830
Size: 2742 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6220
SalI G'TCGA_C
Cuts at: 1936 3562 1936
Size: 1626 4594
SbfI CC_TGCA'GG
Cuts at: 3572 3572
Size: 6220
ScaI AGT'ACT
Cuts at: 4468 6180 4468
Size: 1712 4508
SfcI C'TryA_G
Cuts at: 826 1045 3568 4703 5384 5575 826
Size: 219 2523 1135 681 191 1471
Fragments arranged by size:
2523 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6220
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6220
SmaI CCC'GGG
Cuts at: 1872 3547 1872
Size: 1675 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2054 2483 2898 3504 4324 5225
Size: 197 926 111 429 415 606 820 901
Cuts at: 5225 5487 820
Size: 262 1553
Fragments arranged by size:
1553 926 901 820 606 429 415 262
197 111
SnaBI TAC'GTA
Cuts at: 493 4011 493
Size: 3518 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6220
SphI G_CATG'C
Cuts at: 1132 3578 1132
Size: 2446 3774
SspI AAT'ATT
Cuts at: 5 52 4144 5
Size: 47 4092 2081
Fragments arranged by size:
4092 2081 47
StuI AGG'CCT
Cuts at: 1517 2249 1517
Size: 732 5488
StyI C'CwwG_G
Cuts at: 513 1065 2272 3659 513
Size: 552 1207 1387 3074
Fragments arranged by size:
3074 1387 1207 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4247 4264 4417 4602 5047 325
Size: 1255 2667 17 153 185 445 1498
Fragments arranged by size:
2667 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2685 4466 6178
Size: 274 80 33 51 1443 708 1781 1712
Cuts at: 6178 96
Size: 138
Fragments arranged by size:
1781 1712 1443 708 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2064 2084 2096 2116 6180 6212 2064
Size: 20 12 20 4064 32 2072
Fragments arranged by size:
4064 2072 32 20 20 12
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6220
XbaI T'CTAG_A
Cuts at: 3556 3556
Size: 6220
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2533 2533
Size: 6220
XhoI C'TCGA_G
Cuts at: 1943 2898 1943
Size: 955 5265
XmaI C'CCGG_G
Cuts at: 1870 3545 1870
Size: 1675 4545
XmnI GAAnn'nnTTC
Cuts at: 2354 2357 4349 2354
Size: 3 1992 4225
Fragments arranged by size:
4225 1992 3
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4028 276
Size: 53 83 186 936 2494 2468
Fragments arranged by size:
2494 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI
PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI
PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BsiWI BstZ17I Bsu36I EcoICRI FseI FspAI KasI NarI
NruI PacI PfoI PmlI PpuMI PspXI PsrI PvuII
RsrII SacI SacII SanDI SapI SexAI SfiI SfoI
SrfI SwaI