(Linear) (Six-base) MAPSORT of: KIEE4121.seq Check: 7603 from: 1 to: 1515 KIEE4121.orf With 182 enzymes: SgfI * March 22, 2012 17:24 .. AclI AA'CG_TT Cuts at: 0 1463 1515 Size: 1463 52 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 339 1018 1515 Size: 339 679 497 Fragments arranged by size: 679 497 339 AflII C'TTAA_G Cuts at: 0 49 1515 Size: 49 1466 AflIII A'CryG_T Cuts at: 0 1193 1515 Size: 1193 322 AhdI GACnn_n'nnGTC Cuts at: 0 1155 1515 Size: 1155 360 AlwNI CAG_nnn'CTG Cuts at: 0 798 1515 Size: 798 717 ApaI G_GGCC'C Cuts at: 0 276 1515 Size: 276 1239 ApoI r'AATT_y Cuts at: 0 68 349 389 472 1515 Size: 68 281 40 83 1043 Fragments arranged by size: 1043 281 83 68 40 AvaI C'yCGr_G Cuts at: 0 893 1515 Size: 893 622 BanII G_rGCy'C Cuts at: 0 276 774 1515 Size: 276 498 741 Fragments arranged by size: 741 498 276 BbsI GAAGACnn'nnnn_ Cuts at: 0 2 328 824 1515 Size: 2 326 496 691 Fragments arranged by size: 691 496 326 2 BfrBI ATG'CAT Cuts at: 0 543 1515 Size: 543 972 BglII A'GATC_T Cuts at: 0 744 1515 Size: 744 771 Bme1580I G_kGCm'C Cuts at: 0 276 1515 Size: 276 1239 BmrI ACTGGGnnnn_n' Cuts at: 0 213 404 1149 1515 Size: 213 191 745 366 Fragments arranged by size: 745 366 213 191 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1256 1515 Size: 1256 259 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 177 208 626 1109 1515 Size: 177 31 418 483 406 Fragments arranged by size: 483 418 406 177 31 Bpu10I CC'TnA_GC Cuts at: 0 587 1254 1515 Size: 587 667 261 Fragments arranged by size: 667 587 261 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 463 1484 1515 Size: 463 1021 31 Fragments arranged by size: 1021 463 31 BsaI GGTCTCn'nnnn_ Cuts at: 0 1244 1515 Size: 1244 271 BsaBI GATnn'nnATC Cuts at: 0 1184 1515 Size: 1184 331 BsaHI Gr'CG_yC Cuts at: 0 7 970 1151 1259 1515 Size: 7 963 181 108 256 Fragments arranged by size: 963 256 181 108 7 BseYI C'CCAG_C Cuts at: 0 779 1515 Size: 779 736 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 942 1515 Size: 942 573 BsiHKAI G_wGCw'C Cuts at: 0 588 1515 Size: 588 927 BsmI GAATG_Cn' Cuts at: 0 545 1515 Size: 545 970 BsmBI CGTCTCn'nnnn_ Cuts at: 0 700 1515 Size: 700 815 Bsp1286I G_dGCh'C Cuts at: 0 276 588 774 1515 Size: 276 312 186 741 Fragments arranged by size: 741 312 276 186 BspHI T'CATG_A Cuts at: 0 686 1322 1515 Size: 686 636 193 Fragments arranged by size: 686 636 193 BsrFI r'CCGG_y Cuts at: 0 425 966 1515 Size: 425 541 549 Fragments arranged by size: 549 541 425 BssSI C'ACGA_G Cuts at: 0 626 1515 Size: 626 889 BstEII G'GTnAC_C Cuts at: 0 333 1515 Size: 333 1182 BstXI CCAn_nnnn'nTGG Cuts at: 0 1341 1515 Size: 1341 174 BstYI r'GATC_y Cuts at: 0 87 555 744 1284 1515 Size: 87 468 189 540 231 Fragments arranged by size: 540 468 231 189 87 BtsI GCAGTG_nn' Cuts at: 0 1489 1515 Size: 1489 26 DraIII CAC_nnn'GTG Cuts at: 0 606 920 1515 Size: 606 314 595 Fragments arranged by size: 606 595 314 EaeI y'GGCC_r Cuts at: 0 608 1200 1515 Size: 608 592 315 Fragments arranged by size: 608 592 315 EarI CTCTTCn'nnn_ Cuts at: 0 158 233 1515 Size: 158 75 1282 Fragments arranged by size: 1282 158 75 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 229 1515 Size: 229 1286 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 177 208 339 626 1018 1109 1515 Size: 177 31 131 287 392 91 406 Fragments arranged by size: 406 392 287 177 131 91 31 EcoNI CCTnn'n_nnAGG Cuts at: 0 1212 1515 Size: 1212 303 EcoO109I rG'GnC_Cy Cuts at: 0 272 1515 Size: 272 1243 EcoRI G'AATT_C Cuts at: 0 68 1515 Size: 68 1447 EcoRV GAT'ATC Cuts at: 0 864 1515 Size: 864 651 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 369 951 1515 Size: 369 582 564 Fragments arranged by size: 582 564 369 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 439 471 609 641 651 683 955 987 Size: 439 32 138 32 10 32 272 32 Cuts at: 987 1141 1173 1222 1254 1276 1308 1515 Size: 154 32 49 32 22 32 207 Fragments arranged by size: 439 272 207 154 138 49 32 32 32 32 32 32 32 22 10 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 354 1515 Size: 354 1161 MscI TGG'CCA Cuts at: 0 1202 1515 Size: 1202 313 MslI CAynn'nnrTG Cuts at: 0 871 1515 Size: 871 644 MspA1I CmG'CkG Cuts at: 0 228 976 1515 Size: 228 748 539 Fragments arranged by size: 748 539 228 NaeI GCC'GGC Cuts at: 0 968 1515 Size: 968 547 NgoMIV G'CCGG_C Cuts at: 0 966 1515 Size: 966 549 NsiI A_TGCA'T Cuts at: 0 545 1515 Size: 545 970 NspI r_CATG'y Cuts at: 0 1197 1515 Size: 1197 318 PciI A'CATG_T Cuts at: 0 1193 1515 Size: 1193 322 PflMI CCAn_nnn'nTGG Cuts at: 0 24 155 606 1515 Size: 24 131 451 909 Fragments arranged by size: 909 451 131 24 PspOMI G'GGCC_C Cuts at: 0 272 1515 Size: 272 1243 SmlI C'TyrA_G Cuts at: 0 49 478 893 1499 1515 Size: 49 429 415 606 16 Fragments arranged by size: 606 429 415 49 16 StuI AGG'CCT Cuts at: 0 244 1515 Size: 244 1271 StyI C'CwwG_G Cuts at: 0 267 1515 Size: 267 1248 TatI w'GTAC_w Cuts at: 0 680 1515 Size: 680 835 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 59 79 91 111 1515 Size: 59 20 12 20 1404 Fragments arranged by size: 1404 59 20 20 12 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 528 1515 Size: 528 987 XhoI C'TCGA_G Cuts at: 0 893 1515 Size: 893 622 XmnI GAAnn'nnTTC Cuts at: 0 349 352 1515 Size: 349 3 1163 Fragments arranged by size: 1163 349 3 Enzymes that do cut: AclI AcuI AflII AflIII AhdI AlwNI ApaI ApoI AvaI BanII BbsI BfrBI BglII Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaBI BsaHI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspHI BsrFI BssSI BstEII BstXI BstYI BtsI DraIII EaeI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI Hin4I MmeI MscI MslI MspA1I NaeI NgoMIV NsiI NspI PciI PflMI PspOMI SmlI StuI StyI TatI TstI XcmI XhoI XmnI Enzymes that do not cut: AarI AatII AccI Acc65I AfeI AgeI AleI AlfI AloI ApaLI AscI AseI AsiSI AvrII BaeI BamHI BanI BbeI BbvCI BcgI BciVI BclI BglI BlpI BmgBI BmtI BsaAI BsaWI BsaXI BseRI BsiEI BsiWI BspEI BspMI BsrBI BsrDI BsrGI BssHII BstAPI BstBI BstZ17I Bsu36I BtgI BtgZI ClaI CspCI DraI DrdI EagI EcoICRI FseI FspI FspAI HaeII HincII HindIII HpaI KasI KpnI MfeI MluI NarI NcoI NdeI NheI NotI NruI PacI PasI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PsrI PstI PvuI PvuII RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI SwaI TaqII Tth111I XbaI XmaI ZraI