################################## # NO CUT ENZYMES ( 152 enzymes) # ################################## AatII Acc65I AccIII AclI AcyI AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspI AvaI AviII AvrII BamHI BanI BbeI BbrPI BbsI BbvCI BciVI BclI BcnI BglI BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsiEI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BsrI BssHII Bst1107I BstEII BstPI BstXI BstZ17I Bsu36I Cac8I CelII CfoI Cfr10I ClaI CpoI DraI DraII DraIII DrdI DsaI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV FauI FbaI FseI FspI HaeII HapII HhaI Hin1I HinP1I HindIII HpaI HpaII KasI KpnI Ksp632I KspI MamI MfeI MluI MroI MspI MunI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshBI Psp1406I PvuI RsrII SacII SalI SapI SbfI SexAI SfiI SfoI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SwaI Tth111I Van91I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### AfaI 125 GTAC GT^AC AflII 1114 CTTAAG C^TTAAG AlwNI 1310 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG AvaII 1335 GGACC G^G[AT]CC BalI 1085 TGGCCA TGG^CCA BanII 500 GAGCTC G[GA]GC[CT]^C BcgI 1422 CGATCCTGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BfrI 1114 CTTAAG C^TTAAG BglII 825 AGATCT A^GATCT BpmI 1178 CTGGAG CTGGAG(16/14) BseRI 149 GAGGAG GAGGAG(10/8) BsgI 581 CTGCAC GTGCAG(16/14) BsmAI 264 GTCTC GTCTC(1/5) BspHI 249 TCATGA T^CATGA BssSI 236 CACGAG CACGAG(-5/-1) BstAPI 1309 GCAGCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstBI 1226 TTCGAA TT^CGAA Cfr13I 1335 GGACC G^G[ACGT]CC HgaI 363 GCGTC GACGC(5/10) HincII 154 GTTGAC GT[CT]^[GA]AC HindII 154 GTTGAC GT[CT]^[GA]AC HphI 1298 TCACC GGTGA(8/7) MaeII 517 ACGT A^CGT MluNI 1085 TGGCCA TGG^CCA MscI 1085 TGGCCA TGG^CCA MslI 162 CACCAAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NspV 1226 TTCGAA TT^CGAA PshAI 215 GACGAAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PstI 1316 CTGCAG CTGCA^G RcaI 249 TCATGA T^CATGA RsaI 125 GTAC GT^AC SacI 500 GAGCTC GAGCT^C Sau96I 1335 GGACC G^G[ACGT]CC ScaI 124 AGTACT AGT^ACT SfcI 1316 CTGCAG C^T[GA][CT]AG SfuI 1226 TTCGAA TT^CGAA SmlI 1114 CTTAAG C^T[CT][GA]AG SspI 1019 AATATT AAT^ATT StuI 1174 AGGCCT AGG^CCT XcmI 1395 CCATCCCCCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AccII 134 CGCG CG^CG 362 CGCG AciI 361 CCGC CCGC(-3/-1) 1185 CCGC AcsI 76 AAATTT [GA]^AATT[CT] 880 GAATTT ApoI 76 AAATTT [GA]^AATT[CT] 880 GAATTT Asp700I 122 GAAGTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 680 GAATTGGTTC AspHI 500 GAGCTC G[AT]GC[AT]^C 863 GTGCTC BsiHKAI 500 GAGCTC G[AT]GC[AT]^C 863 GTGCTC BsmFI 192 GGGAC GGGAC(10/14) 54 GTCCC BstUI 134 CGCG CG^CG 362 CGCG BstYI 616 GGATCT [GA]^GATC[CT] 825 AGATCT EaeI 621 TGGCCG [CT]^GGCC[GA] 1085 TGGCCA EcoT14I 5 CCAAGG C^C[AT][AT]GG 104 CCTTGG EcoT22I 841 ATGCAT ATGCA^T 1351 ATGCAT MflI 616 GGATCT [GA]^GATC[CT] 825 AGATCT MseI 815 TTAA T^TAA 1115 TTAA MvnI 134 CGCG CG^CG 362 CGCG NlaIV 388 GGTTCC GG[ACGT]^[ACGT]CC 685 GGTTCC NsiI 841 ATGCAT ATGCA^T 1351 ATGCAT NspI 775 GCATGT [GA]CATG^[CT] 1263 GCATGT StyI 5 CCAAGG C^C[AT][AT]GG 104 CCTTGG Tru9I 815 TTAA T^TAA 1115 TTAA XhoII 616 GGATCT [GA]^GATC[CT] 825 AGATCT XmnI 122 GAAGTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 680 GAATTGGTTC ######################################### # 3 OR MORE CUT ENZYMES ( 43 enzymes) # ######################################### AccI AluI AlwI BbvI BfaI BmyI BsaJI BsiYI BslI Bsp1286I BssKI BstF5I BstNI DdeI DpnII EcoRII Fnu4HI FokI HaeIII HinfI ItaI MaeI MaeIII MboI MboII MnlI MspA1I MvaI MwoI NdeII NlaIII PleI PvuII Sau3AI ScrFI SfaNI TaqI TfiI TseI Tsp45I Tsp509I TspRI TthHB8I