(Circular) (Six-base) MAPSORT of: pFN21AE4112.seq Check: 5633 from: 1 to: 6154 pFN21AE4112 6154 bp With 182 enzymes: SgfI * March 28, 2012 13:17 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3964 278 Size: 53 83 186 936 2428 2468 Fragments arranged by size: 2468 2428 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2105 2179 2568 2875 3273 3497 1937 Size: 168 74 389 307 398 224 4594 Fragments arranged by size: 4594 398 389 307 224 168 74 Acc65I G'GTAC_C Cuts at: 3475 3475 Size: 6154 AclI AA'CG_TT Cuts at: 1563 4281 4654 1563 Size: 2718 373 3063 Fragments arranged by size: 3063 2718 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4217 5601 5869 1632 Size: 2585 1384 268 1917 Fragments arranged by size: 2585 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 3119 820 Size: 197 2102 3855 Fragments arranged by size: 3855 2102 197 AflIII A'CryG_T Cuts at: 1203 5053 6030 1203 Size: 3850 977 1327 Fragments arranged by size: 3850 1327 977 AgeI A'CCGG_T Cuts at: 4985 4985 Size: 6154 AhdI GACnn_n'nnGTC Cuts at: 4883 4883 Size: 6154 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6154 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6154 AlwNI CAG_nnn'CTG Cuts at: 3320 3897 5469 3320 Size: 577 1572 4005 Fragments arranged by size: 4005 1572 577 ApaLI G'TGCA_C Cuts at: 4211 5367 4211 Size: 1156 4998 ApoI r'AATT_y Cuts at: 1493 2081 2885 3463 3689 3841 5950 1493 Size: 588 804 578 226 152 2109 1697 Fragments arranged by size: 2109 1697 804 588 578 226 152 AseI AT'TA_AT Cuts at: 160 1032 4708 160 Size: 872 3676 1606 Fragments arranged by size: 3676 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6154 AvaI C'yCGr_G Cuts at: 1870 1943 3479 1870 Size: 73 1536 4545 Fragments arranged by size: 4545 1536 73 BamHI G'GATC_C Cuts at: 3484 3851 3484 Size: 367 5787 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3475 4930 618 Size: 325 217 627 1688 1455 1842 Fragments arranged by size: 1842 1688 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2509 3473 1391 Size: 1118 964 4072 Fragments arranged by size: 4072 1118 964 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5183 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3416 3450 4345 4379 1903 Size: 34 50 34 1395 34 895 34 3678 Fragments arranged by size: 3678 1395 895 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4046 5256 4046 Size: 1210 4944 BclI T'GATC_A Cuts at: 1548 1548 Size: 6154 BfrBI ATG'CAT Cuts at: 2848 3358 2848 Size: 510 5644 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4765 136 Size: 107 122 71 631 605 3093 1525 Fragments arranged by size: 3093 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2830 6149 2830 Size: 3319 2835 BlpI GC'TnA_GC Cuts at: 3571 3571 Size: 6154 Bme1580I G_kGCm'C Cuts at: 1790 2842 2932 4215 5371 1790 Size: 1052 90 1283 1156 2573 Fragments arranged by size: 2573 1283 1156 1052 90 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6154 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3885 4843 442 Size: 416 53 83 399 24 2468 958 1753 Fragments arranged by size: 2468 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6154 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3204 4814 1239 Size: 1965 1610 2579 Fragments arranged by size: 2579 1965 1610 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4279 5144 5442 4279 Size: 865 298 4991 Fragments arranged by size: 4991 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4817 882 Size: 617 3318 2219 Fragments arranged by size: 3318 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3945 493 Size: 711 2741 2702 Fragments arranged by size: 2741 2702 711 BsaBI GATnn'nnATC Cuts at: 6148 6148 Size: 6154 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3961 4343 4978 275 Size: 53 83 186 936 2428 382 635 1451 Fragments arranged by size: 2428 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4587 4985 5259 5406 5817 4587 Size: 398 274 147 411 4924 Fragments arranged by size: 4924 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2462 2492 1178 Size: 30 165 30 1059 30 4840 Fragments arranged by size: 4840 1059 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2169 1186 Size: 983 5171 BseYI C'CCAG_C Cuts at: 2408 2465 5357 5853 2408 Size: 57 2892 496 2709 Fragments arranged by size: 2892 2709 496 57 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2571 1163 Size: 573 835 4746 Fragments arranged by size: 4746 835 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3604 4365 5393 1540 Size: 275 186 1603 761 1028 2301 Fragments arranged by size: 2301 1603 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2509 2872 4215 4300 5371 2509 Size: 363 1343 85 1071 3292 Fragments arranged by size: 3292 1343 1071 363 85 BsmI GAATG_Cn' Cuts at: 3665 3758 3665 Size: 93 6061 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2509 2842 2872 2932 3473 4215 4300 Size: 399 719 333 30 60 541 742 85 Cuts at: 4300 5371 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 719 541 399 333 85 60 30 BspEI T'CCGG_A Cuts at: 5817 5817 Size: 6154 BspHI T'CATG_A Cuts at: 2254 4043 5773 2254 Size: 1789 1730 2635 Fragments arranged by size: 2635 1789 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3509 844 Size: 2665 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4041 1394 Size: 429 2218 3507 Fragments arranged by size: 3507 2218 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4649 4823 65 Size: 1183 3401 174 1396 Fragments arranged by size: 3401 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4798 4985 1700 Size: 110 2988 187 2869 Fragments arranged by size: 2988 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6154 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6154 BssSI C'ACGA_G Cuts at: 2241 4214 5226 2241 Size: 1973 1012 3169 Fragments arranged by size: 3169 1973 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3320 794 Size: 2526 3628 BstBI TT'CG_AA Cuts at: 3232 4958 3232 Size: 1726 4428 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6154 BstYI r'GATC_y Cuts at: 1972 2621 2830 3484 3851 4236 4253 5705 5791 Size: 649 209 654 367 385 17 1452 86 Cuts at: 5791 5803 6149 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 654 649 385 367 346 209 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5602 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5415 Fragments arranged by size: 5415 683 56 BtsI GCAGTG_nn' Cuts at: 2410 3670 4464 4484 2410 Size: 1260 794 20 4080 Fragments arranged by size: 4080 1260 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6154 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6119 DraI TTT'AAA Cuts at: 3458 3458 Size: 6154 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5161 809 Size: 507 3845 1802 Fragments arranged by size: 3845 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2626 3090 3601 Size: 54 1425 50 275 18 796 464 511 Cuts at: 3601 4490 6119 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 796 511 464 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3601 1537 Size: 275 1789 4090 Fragments arranged by size: 4090 1789 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4084 1353 Size: 2731 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4741 5115 5261 387 Size: 1064 138 3152 374 146 1280 Fragments arranged by size: 3152 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2507 2507 Size: 6154 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3204 4217 4814 5601 5869 1239 Size: 393 1572 1013 597 787 268 1524 Fragments arranged by size: 1572 1524 1013 787 597 393 268 EcoRI G'AATT_C Cuts at: 3463 3841 3463 Size: 378 5776 FspI TGC'GCA Cuts at: 4660 4660 Size: 6154 HaeII r_GCGC'y Cuts at: 1678 5301 1678 Size: 3623 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2741 2773 2896 2928 4795 Size: 32 135 32 1169 32 123 32 1867 Cuts at: 4795 4827 4869 4901 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1867 1169 135 123 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2161 3498 3744 669 Size: 988 281 223 1337 246 3079 Fragments arranged by size: 3079 1337 988 281 246 223 HindIII A'AGCT_T Cuts at: 748 748 Size: 6154 HpaI GTT'AAC Cuts at: 3744 3744 Size: 6154 KpnI G_GTAC'C Cuts at: 3479 3479 Size: 6154 MfeI C'AATT_G Cuts at: 3753 3753 Size: 6154 MluI A'CGCG_T Cuts at: 6030 6030 Size: 6154 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2330 3386 5268 5452 1305 Size: 1025 1056 1882 184 2007 Fragments arranged by size: 2007 1882 1056 1025 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3092 10 Size: 54 1425 343 1260 3072 Fragments arranged by size: 3072 1425 1260 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2171 4112 4471 4630 518 Size: 648 1005 1941 359 159 2042 Fragments arranged by size: 2042 1941 1005 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2465 3320 3346 3570 4247 5395 5640 1606 Size: 859 855 26 224 677 1148 245 2120 Fragments arranged by size: 2120 1148 859 855 677 245 224 26 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5602 NdeI CA'TA_TG Cuts at: 387 387 Size: 6154 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6154 NotI GC'GGCC_GC Cuts at: 3601 3601 Size: 6154 NsiI A_TGCA'T Cuts at: 2850 3360 2850 Size: 510 5644 NspI r_CATG'y Cuts at: 1132 1229 2784 3272 3512 5057 1132 Size: 97 1555 488 240 1545 2229 Fragments arranged by size: 2229 1555 1545 488 240 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6154 PciI A'CATG_T Cuts at: 5053 5053 Size: 6154 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6154 PmeI GTTT'AAAC Cuts at: 3458 3458 Size: 6154 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2254 2286 4223 4255 5736 5768 2254 Size: 32 1937 32 1481 32 2640 Fragments arranged by size: 2640 1937 1481 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2224 1613 Size: 611 5543 PsiI TTA'TAA Cuts at: 3724 3724 Size: 6154 PstI C_TGCA'G Cuts at: 830 3325 3506 830 Size: 2495 181 3478 Fragments arranged by size: 3478 2495 181 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6154 PvuII CAG'CTG Cuts at: 2465 3320 3346 2465 Size: 855 26 5273 Fragments arranged by size: 5273 855 26 SacI G_AGCT'C Cuts at: 2509 2509 Size: 6154 SalI G'TCGA_C Cuts at: 1936 3496 1936 Size: 1560 4594 SbfI CC_TGCA'GG Cuts at: 3506 3506 Size: 6154 ScaI AGT'ACT Cuts at: 2131 4402 6114 2131 Size: 2271 1712 2171 Fragments arranged by size: 2271 2171 1712 SfcI C'TryA_G Cuts at: 826 1045 3321 3502 4637 5318 5509 826 Size: 219 2276 181 1135 681 191 1471 Fragments arranged by size: 2276 1471 1135 681 219 191 181 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6154 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6154 SmaI CCC'GGG Cuts at: 1872 3481 1872 Size: 1609 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3119 4258 5159 5421 820 Size: 197 926 1176 1139 901 262 1553 Fragments arranged by size: 1553 1176 1139 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3945 493 Size: 3452 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6154 SphI G_CATG'C Cuts at: 1132 3512 1132 Size: 2380 3774 SspI AAT'ATT Cuts at: 5 52 3026 4078 5 Size: 47 2974 1052 2081 Fragments arranged by size: 2974 2081 1052 47 StuI AGG'CCT Cuts at: 1517 3181 1517 Size: 1664 4490 StyI C'CwwG_G Cuts at: 513 1065 2010 2109 3593 513 Size: 552 945 99 1484 3074 Fragments arranged by size: 3074 1484 945 552 99 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4181 4198 4351 4536 4981 325 Size: 1255 2601 17 153 185 445 1498 Fragments arranged by size: 2601 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2129 4400 6112 Size: 274 80 33 51 1443 152 2271 1712 Cuts at: 6112 96 Size: 138 Fragments arranged by size: 2271 1712 1443 274 152 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6114 6146 6114 Size: 32 6122 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6154 XbaI T'CTAG_A Cuts at: 3490 3490 Size: 6154 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3407 3407 Size: 6154 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6154 XmaI C'CCGG_G Cuts at: 1870 3479 1870 Size: 1609 4545 XmnI GAAnn'nnTTC Cuts at: 2131 2689 4283 2131 Size: 558 1594 4002 Fragments arranged by size: 4002 1594 558 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3962 276 Size: 53 83 186 936 2428 2468 Fragments arranged by size: 2468 2428 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI