(Circular) (Six-base) MAPSORT of: pFN21AE3165.seq Check: 9028 from: 1 to: 5866 pFN21AE3165 5866 bp With 182 enzymes: SgfI * March 28, 2012 13:16 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3676 278 Size: 53 83 186 936 2140 2468 Fragments arranged by size: 2468 2140 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2785 3209 1937 Size: 848 424 4594 Fragments arranged by size: 4594 848 424 Acc65I G'GTAC_C Cuts at: 3187 3187 Size: 5866 AclI AA'CG_TT Cuts at: 1563 3993 4366 1563 Size: 2430 373 3063 Fragments arranged by size: 3063 2430 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2038 2586 2910 3929 5313 5581 1632 Size: 406 548 324 1019 1384 268 1917 Fragments arranged by size: 1917 1384 1019 548 406 324 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5669 AflIII A'CryG_T Cuts at: 1203 4765 5742 1203 Size: 3562 977 1327 Fragments arranged by size: 3562 1327 977 AgeI A'CCGG_T Cuts at: 4697 4697 Size: 5866 AhdI GACnn_n'nnGTC Cuts at: 2350 4595 2350 Size: 2245 3621 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5866 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2778 1184 Size: 1594 4272 AlwNI CAG_nnn'CTG Cuts at: 2061 2094 3095 3609 5181 2061 Size: 33 1001 514 1572 2746 Fragments arranged by size: 2746 1572 1001 514 33 ApaLI G'TGCA_C Cuts at: 3923 5079 3923 Size: 1156 4710 ApoI r'AATT_y Cuts at: 1493 2693 2921 3175 3401 3553 5662 1493 Size: 1200 228 254 226 152 2109 1697 Fragments arranged by size: 2109 1697 1200 254 228 226 152 AseI AT'TA_AT Cuts at: 160 1032 4420 160 Size: 872 3388 1606 Fragments arranged by size: 3388 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5866 AvaI C'yCGr_G Cuts at: 1870 1943 3191 1870 Size: 73 1248 4545 Fragments arranged by size: 4545 1248 73 BamHI G'GATC_C Cuts at: 3196 3563 3196 Size: 367 5499 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3187 4642 618 Size: 325 217 627 1400 1455 1842 Fragments arranged by size: 1842 1455 1400 627 325 217 BanII G_rGCy'C Cuts at: 1391 3185 1391 Size: 1794 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2105 2328 2592 2955 2977 928 Size: 971 206 223 264 363 22 3817 Fragments arranged by size: 3817 971 363 264 223 206 22 BbvCI CC'TCA_GC Cuts at: 2087 2087 Size: 5866 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4057 4091 1903 Size: 34 2120 34 3678 Fragments arranged by size: 3678 2120 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3079 3758 4968 3079 Size: 679 1210 3977 Fragments arranged by size: 3977 1210 679 BclI T'GATC_A Cuts at: 1548 2517 2803 1548 Size: 969 286 4611 Fragments arranged by size: 4611 969 286 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2638 4477 136 Size: 107 122 71 631 605 966 1839 1525 Fragments arranged by size: 1839 1525 966 631 605 122 107 71 BglII A'GATC_T Cuts at: 2362 5861 2362 Size: 3499 2367 BlpI GC'TnA_GC Cuts at: 3283 3283 Size: 5866 Bme1580I G_kGCm'C Cuts at: 1790 3927 5083 1790 Size: 2137 1156 2573 Fragments arranged by size: 2573 2137 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5866 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2071 3597 4555 Size: 416 53 83 399 24 654 1526 958 Cuts at: 4555 442 Size: 1753 Fragments arranged by size: 1753 1526 958 654 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5866 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2093 4526 1239 Size: 854 2433 2579 Fragments arranged by size: 2579 2433 854 Bpu10I CC'TnA_GC Cuts at: 2087 2087 Size: 5866 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2985 3991 4856 5154 2985 Size: 1006 865 298 3697 Fragments arranged by size: 3697 1006 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4529 882 Size: 617 3030 2219 Fragments arranged by size: 3030 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3657 493 Size: 711 2453 2702 Fragments arranged by size: 2702 2453 711 BsaBI GATnn'nnATC Cuts at: 5860 5860 Size: 5866 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3673 4055 4690 275 Size: 53 83 186 936 2140 382 635 1451 Fragments arranged by size: 2140 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4299 4697 4971 5118 5529 4299 Size: 398 274 147 411 4636 Fragments arranged by size: 4636 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5641 Fragments arranged by size: 5641 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2168 1186 Size: 982 4884 BseYI C'CCAG_C Cuts at: 5069 5565 5069 Size: 496 5370 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2784 1163 Size: 573 1048 4245 Fragments arranged by size: 4245 1048 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3316 4077 5105 1540 Size: 275 186 1315 761 1028 2301 Fragments arranged by size: 2301 1315 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3927 4012 5083 3927 Size: 85 1071 4710 Fragments arranged by size: 4710 1071 85 BsiWI C'GTAC_G Cuts at: 2138 2138 Size: 5866 BsmI GAATG_Cn' Cuts at: 3024 3377 3470 3024 Size: 353 93 5420 Fragments arranged by size: 5420 353 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3185 3927 4012 5083 1391 Size: 399 1395 742 85 1071 2174 Fragments arranged by size: 2174 1395 1071 742 399 85 BspEI T'CCGG_A Cuts at: 5529 5529 Size: 5866 BspHI T'CATG_A Cuts at: 2233 3040 3755 5485 2233 Size: 807 715 1730 2614 Fragments arranged by size: 2614 1730 807 715 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3221 844 Size: 2377 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3753 1394 Size: 429 1930 3507 Fragments arranged by size: 3507 1930 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2946 4361 4535 65 Size: 1183 1698 1415 174 1396 Fragments arranged by size: 1698 1415 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2817 4510 4697 1700 Size: 110 1007 1693 187 2869 Fragments arranged by size: 2869 1693 1007 187 110 BsrGI T'GTAC_A Cuts at: 96 2200 96 Size: 2104 3762 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5866 BssSI C'ACGA_G Cuts at: 3926 4938 3926 Size: 1012 4854 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5866 BstBI TT'CG_AA Cuts at: 4670 4670 Size: 5866 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5866 BstXI CCAn_nnnn'nTGG Cuts at: 2298 2298 Size: 5866 BstYI r'GATC_y Cuts at: 1972 2287 2320 2362 2947 3196 3563 3948 3965 Size: 315 33 42 585 249 367 385 17 Cuts at: 3965 5417 5503 5515 5861 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 585 385 367 346 315 249 86 42 33 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 3071 513 Size: 552 939 1067 3308 Fragments arranged by size: 3308 1067 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5127 Fragments arranged by size: 5127 683 56 BtsI GCAGTG_nn' Cuts at: 3382 4176 4196 3382 Size: 794 20 5052 Fragments arranged by size: 5052 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5866 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5831 DraI TTT'AAA Cuts at: 3170 3170 Size: 5866 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4873 809 Size: 507 3557 1802 Fragments arranged by size: 3557 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2079 2169 3313 Size: 54 1425 50 275 18 249 90 1144 Cuts at: 3313 4202 5831 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1144 889 275 249 90 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3313 1537 Size: 275 1501 4090 Fragments arranged by size: 4090 1501 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2361 2452 2745 3796 1353 Size: 1008 91 293 1051 3423 Fragments arranged by size: 3423 1051 1008 293 91 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4453 4827 4973 387 Size: 1064 138 2864 374 146 1280 Fragments arranged by size: 2864 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2038 2093 2586 2910 3929 4526 5313 Size: 393 406 55 493 324 1019 597 787 Cuts at: 5313 5581 1239 Size: 268 1524 Fragments arranged by size: 1524 1019 787 597 493 406 393 324 268 55 EcoRI G'AATT_C Cuts at: 3175 3553 3175 Size: 378 5488 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2478 2478 Size: 5866 FspI TGC'GCA Cuts at: 4372 4372 Size: 5866 HaeII r_GCGC'y Cuts at: 1678 5013 1678 Size: 3335 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2336 2368 2649 2681 2767 Size: 32 135 32 764 32 281 32 86 Cuts at: 2767 2799 4507 4539 4581 4613 1373 Size: 32 1708 32 42 32 2626 Fragments arranged by size: 2626 1708 764 281 135 86 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2344 2770 2786 3210 3456 669 Size: 988 281 406 426 16 424 246 3079 Fragments arranged by size: 3079 988 426 424 406 281 246 16 HindIII A'AGCT_T Cuts at: 748 748 Size: 5866 HpaI GTT'AAC Cuts at: 2770 3456 2770 Size: 686 5180 KpnI G_GTAC'C Cuts at: 3191 3191 Size: 5866 MfeI C'AATT_G Cuts at: 3465 3465 Size: 5866 MluI A'CGCG_T Cuts at: 5742 5742 Size: 5866 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4980 5164 1305 Size: 3675 184 2007 Fragments arranged by size: 3675 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4044 Fragments arranged by size: 4044 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2760 3824 4183 4342 518 Size: 648 1594 1064 359 159 2042 Fragments arranged by size: 2042 1594 1064 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2028 2091 2624 3282 3959 5107 5352 1606 Size: 422 63 533 658 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 658 533 422 245 63 NcoI C'CATG_G Cuts at: 513 1065 2004 3071 513 Size: 552 939 1067 3308 Fragments arranged by size: 3308 1067 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5866 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5866 NotI GC'GGCC_GC Cuts at: 3313 3313 Size: 5866 NspI r_CATG'y Cuts at: 1132 1229 3224 4769 1132 Size: 97 1995 1545 2229 Fragments arranged by size: 2229 1995 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5866 PciI A'CATG_T Cuts at: 4765 4765 Size: 5866 PflMI CCAn_nnn'nTGG Cuts at: 1108 2624 2957 1108 Size: 1516 333 4017 Fragments arranged by size: 4017 1516 333 PfoI T'CCnGG_A Cuts at: 3081 3081 Size: 5866 PmeI GTTT'AAAC Cuts at: 3170 3170 Size: 5866 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3935 3967 5448 5480 3935 Size: 32 1481 32 4321 Fragments arranged by size: 4321 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5866 PsiI TTA'TAA Cuts at: 3436 3436 Size: 5866 PstI C_TGCA'G Cuts at: 830 3218 830 Size: 2388 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5866 PvuII CAG'CTG Cuts at: 2624 2624 Size: 5866 SalI G'TCGA_C Cuts at: 1936 2784 3208 1936 Size: 848 424 4594 Fragments arranged by size: 4594 848 424 SapI GCTCTTCn'nnn_ Cuts at: 2452 2745 2452 Size: 293 5573 SbfI CC_TGCA'GG Cuts at: 3218 3218 Size: 5866 ScaI AGT'ACT Cuts at: 4114 5826 4114 Size: 1712 4154 SexAI A'CCwGG_T Cuts at: 2554 2554 Size: 5866 SfcI C'TryA_G Cuts at: 826 1045 2431 3214 4349 5030 5221 826 Size: 219 1386 783 1135 681 191 1471 Fragments arranged by size: 1471 1386 1135 783 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5866 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5866 SmaI CCC'GGG Cuts at: 1872 3193 1872 Size: 1321 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3000 3970 4871 5133 820 Size: 197 926 1057 970 901 262 1553 Fragments arranged by size: 1553 1057 970 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3657 493 Size: 3164 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5866 SphI G_CATG'C Cuts at: 1132 3224 1132 Size: 2092 3774 SspI AAT'ATT Cuts at: 5 52 3790 5 Size: 47 3738 2081 Fragments arranged by size: 3738 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5866 StyI C'CwwG_G Cuts at: 513 1065 2004 3071 3305 513 Size: 552 939 1067 234 3074 Fragments arranged by size: 3074 1067 939 552 234 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3084 3893 3910 4063 4248 4693 325 Size: 1255 1504 809 17 153 185 445 1498 Fragments arranged by size: 1504 1498 1255 809 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2200 2255 4112 Size: 274 80 33 51 1443 223 55 1857 Cuts at: 4112 5824 96 Size: 1712 138 Fragments arranged by size: 1857 1712 1443 274 223 138 80 55 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5826 5858 5826 Size: 32 5834 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5866 XbaI T'CTAG_A Cuts at: 2365 3202 2365 Size: 837 5029 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5866 XmaI C'CCGG_G Cuts at: 1870 3191 1870 Size: 1321 4545 XmnI GAAnn'nnTTC Cuts at: 2102 2461 3995 2102 Size: 359 1534 3973 Fragments arranged by size: 3973 1534 359 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3674 276 Size: 53 83 186 936 2140 2468 Fragments arranged by size: 2468 2140 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SfiI SfoI SrfI SwaI XcmI