################################## # NO CUT ENZYMES ( 121 enzymes) # ################################## Acc65I AccI AccIII AclI AflII AgeI Alw44I Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspHI AviII AvrII BbrPI BbvCI BcgI BciVI BclI BfrI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaWI BseAI BsiHKAI BsiWI BsmBI BsmFI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I CelII Cfr10I ClaI CpoI DraI DraIII DrdI EagI EclXI Eco47III Eco52I EcoNI EcoO65I EcoRV EcoT22I FbaI FseI FspI HgaI HindIII KpnI MamI MfeI MluI MroI MunI NaeI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PpuMI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SalI SapI SbfI ScaI SexAI SfiI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI StuI SwaI Tsp45I XbaI XcmI XhoI ################################### # ONE CUT ENZYMES ( 53 enzymes) # ################################### AatII 349 GACGTC GACGT^C AcsI 328 GAATTC [GA]^AATT[CT] AflIII 644 ACGTGT A^C[GA][CT]GT AhdI 59 GACTAGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AluI 467 AGCT AG^CT AlwNI 812 CAGCCCCTG CAG[ACGT][ACGT][ACGT]^CTG ApoI 328 GAATTC [GA]^AATT[CT] Asp700I 754 GAAGCAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 59 GACTAGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 5 GACCGGGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaII 674 GGACC G^G[AT]CC BalI 396 TGGCCA TGG^CCA BanII 195 GAGCCC G[GA]GC[CT]^C BbsI 312 GAAGAC GAAGAC(2/6) BfaI 61 CTAG C^TAG BglI 154 GCCGCCCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BmyI 195 GAGCCC G[AGT]GC[ACT]^C BpmI 896 CTCCAG CTGGAG(16/14) BpuAI 312 GAAGAC GAAGAC(2/6) BsaI 10 GGTCTC GGTCTC(1/5) BsiEI 4 CGACCG CG[GA][CT]^CG Bsp1286I 195 GAGCCC G[AGT]GC[ACT]^C BsrBI 113 CCGCTC CCGCTC(-3/-3) BsrDI 913 CATTGC GCAATG(2/0) BsrI 623 ACTGG ACTGG(1/-1) BstF5I 854 GGATG GGATG(2/0) Cac8I 115 GCTCGC GC[ACGT]^[ACGT]GC DraII 207 AGGCCCC [GA]G^G[ACGT]CC[CT] Eam1105I 59 GACTAGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoO109I 207 AGGCCCC [GA]G^G[ACGT]CC[CT] EcoRI 328 GAATTC G^AATTC EcoT14I 806 CCATGG C^C[AT][AT]GG FokI 854 GGATG GGATG(9/13) HincII 360 GTTAAC GT[CT]^[GA]AC HindII 360 GTTAAC GT[CT]^[GA]AC HpaI 360 GTTAAC GTT^AAC KspI 217 CCGCGG CCGC^GG MaeI 61 CTAG C^TAG MaeIII 692 GTTAC ^GT[ACGT]AC MluNI 396 TGGCCA TGG^CCA MscI 396 TGGCCA TGG^CCA NcoI 806 CCATGG C^CATGG NspI 319 ACATGC [GA]CATG^[CT] PflFI 5 GACCGGGTC GAC[ACGT]^[ACGT][ACGT]GTC PleI 79 GACTC GAGTC(4/5) PshAI 345 GACAGACGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SacII 217 CCGCGG CCGC^GG SfcI 451 CTACAG C^T[GA][CT]AG SmlI 630 CTTGAG C^T[CT][GA]AG StyI 806 CCATGG C^C[AT][AT]GG TspRI 278 CAGTG CA[GC]TG[ACGT][ACGT]^ Tth111I 5 GACCGGGTC GAC[ACGT]^[ACGT][ACGT]GTC XmnI 754 GAAGCAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AfaI 420 GTAC GT^AC 511 GTAC AocI 203 CCTCAGG CC^T[ACGT]AGG 496 CCTCAGG BamHI 34 GGATCC G^GATCC 109 GGATCC BanI 27 GGCGCC G^G[CT][GA]CC 44 GGCGCC BbeI 27 GGCGCC GGCGC^C 44 GGCGCC BseRI 724 GAGGAG GAGGAG(10/8) 775 GAGGAG BsgI 226 GTGCAG GTGCAG(16/14) 463 GTGCAG BsmAI 685 GAGAC GTCTC(1/5) 11 GTCTC Bsu36I 203 CCTCAGG CC^T[ACGT]AGG 496 CCTCAGG EaeI 136 TGGCCG [CT]^GGCC[GA] 396 TGGCCA EarI 708 GAAGAG CTCTTC(1/4) 721 GAAGAG Eco81I 203 CCTCAGG CC^T[ACGT]AGG 496 CCTCAGG FauI 144 GCGGG CCCGC(4/6) 191 GCGGG KasI 27 GGCGCC G^GCGCC 44 GGCGCC Ksp632I 708 GAAGAG CTCTTC(1/4) 721 GAAGAG MaeII 350 ACGT A^CGT 644 ACGT MslI 639 CATGGACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 766 CATCTCCATG NarI 27 GGCGCC GG^CGCC 44 GGCGCC PflMI 49 CCAACGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 840 CCACCACATGG RsaI 420 GTAC GT^AC 511 GTAC SfoI 27 GGCGCC GGC^GCC 44 GGCGCC SspI 298 AATATT AAT^ATT 888 AATATT TfiI 531 GATTC G^A[AT]TC 903 GAATC Tsp509I 329 AATT ^AATT 759 AATT Van91I 49 CCAACGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 840 CCACCACATGG ######################################### # 3 OR MORE CUT ENZYMES ( 59 enzymes) # ######################################### AccII AciI AcyI AlwI AvaI BbvI BcnI BsaHI BsaJI BsiYI BslI BsoBI BssKI BstNI BstUI BstYI CfoI Cfr13I DdeI DpnII DsaI EcoRII Fnu4HI HaeII HaeIII HapII HhaI Hin1I HinP1I HinfI HpaII HphI ItaI MboI MboII MflI MnlI MseI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV Sau3AI Sau96I ScrFI SfaNI SmaI TaqI Tru9I TseI TthHB8I XhoII XmaCI XmaI