(Circular) (Six-base) MAPSORT of: pFN21AE3121.seq Check: 8868 from: 1 to: 5626 pFN21AE3121 5626 bp With 182 enzymes: SgfI * March 28, 2012 13:15 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2358 3436 278 Size: 53 83 186 936 822 1078 2468 Fragments arranged by size: 2468 1078 936 822 186 83 53 AccI GT'mk_AC Cuts at: 1937 2969 1937 Size: 1032 4594 Acc65I G'GTAC_C Cuts at: 2947 2947 Size: 5626 AclI AA'CG_TT Cuts at: 1563 3753 4126 1563 Size: 2190 373 3063 Fragments arranged by size: 3063 2190 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3689 5073 5341 1632 Size: 2057 1384 268 1917 Fragments arranged by size: 2057 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5429 AflIII A'CryG_T Cuts at: 1203 2649 4525 5502 1203 Size: 1446 1876 977 1327 Fragments arranged by size: 1876 1446 1327 977 AgeI A'CCGG_T Cuts at: 4457 4457 Size: 5626 AhdI GACnn_n'nnGTC Cuts at: 2069 4355 2069 Size: 2286 3340 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5626 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5626 AlwNI CAG_nnn'CTG Cuts at: 2822 3369 4941 2822 Size: 547 1572 3507 Fragments arranged by size: 3507 1572 547 ApaLI G'TGCA_C Cuts at: 3683 4839 3683 Size: 1156 4470 ApoI r'AATT_y Cuts at: 1493 2333 2935 3161 3313 5422 1493 Size: 840 602 226 152 2109 1697 Fragments arranged by size: 2109 1697 840 602 226 152 AseI AT'TA_AT Cuts at: 160 1032 4180 160 Size: 872 3148 1606 Fragments arranged by size: 3148 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5626 AvaI C'yCGr_G Cuts at: 1870 1943 2103 2163 2216 2951 1870 Size: 73 160 60 53 735 4545 Fragments arranged by size: 4545 735 160 73 60 53 BamHI G'GATC_C Cuts at: 2039 2114 2956 3323 2039 Size: 75 842 367 4342 Fragments arranged by size: 4342 842 367 75 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2032 2049 2947 4402 618 Size: 325 217 627 245 17 898 1455 1842 Fragments arranged by size: 1842 1455 898 627 325 245 217 17 BanII G_rGCy'C Cuts at: 1391 2204 2945 1391 Size: 813 741 4072 Fragments arranged by size: 4072 813 741 BbeI G_GCGC'C Cuts at: 2036 2053 2036 Size: 17 5609 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2324 928 Size: 971 425 4230 Fragments arranged by size: 4230 971 425 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3817 3851 1903 Size: 34 50 34 1796 34 3678 Fragments arranged by size: 3678 1796 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3518 4728 3518 Size: 1210 4416 BclI T'GATC_A Cuts at: 1548 1548 Size: 5626 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2165 4237 136 Size: 107 122 71 631 605 493 2072 1525 Fragments arranged by size: 2072 1525 631 605 493 122 107 71 BglII A'GATC_T Cuts at: 5621 5621 Size: 5626 BlpI GC'TnA_GC Cuts at: 3043 3043 Size: 5626 Bme1580I G_kGCm'C Cuts at: 1790 3687 4843 1790 Size: 1897 1156 2573 Fragments arranged by size: 2573 1897 1156 BmgBI CAC'GTC Cuts at: 1318 2650 1318 Size: 1332 4294 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2918 3357 4315 Size: 416 53 83 399 24 1501 439 958 Cuts at: 4315 442 Size: 1753 Fragments arranged by size: 1753 1501 958 439 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5626 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2886 4286 1239 Size: 1647 1400 2579 Fragments arranged by size: 2579 1647 1400 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2656 3751 4616 4914 2656 Size: 1095 865 298 3368 Fragments arranged by size: 3368 1095 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2021 4289 882 Size: 617 522 2268 2219 Fragments arranged by size: 2268 2219 617 522 BsaAI yAC'GTr Cuts at: 493 1204 3417 493 Size: 711 2213 2702 Fragments arranged by size: 2702 2213 711 BsaBI GATnn'nnATC Cuts at: 5620 5620 Size: 5626 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2033 2050 2355 3433 Size: 53 83 186 936 500 17 305 1078 Cuts at: 3433 3815 4450 275 Size: 382 635 1451 Fragments arranged by size: 1451 1078 936 635 500 382 305 186 83 53 17 BsaWI w'CCGG_w Cuts at: 4059 4457 4731 4878 5289 4059 Size: 398 274 147 411 4396 Fragments arranged by size: 4396 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2363 2393 2846 2876 1178 Size: 30 165 30 960 30 453 30 3928 Fragments arranged by size: 3928 960 453 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2744 2795 1186 Size: 1558 51 4017 Fragments arranged by size: 4017 1558 51 BseYI C'CCAG_C Cuts at: 2154 2442 4829 5325 2154 Size: 288 2387 496 2455 Fragments arranged by size: 2455 2387 496 288 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2252 2489 1163 Size: 573 516 237 4300 Fragments arranged by size: 4300 573 516 237 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2012 3076 3837 4865 1540 Size: 275 186 11 1064 761 1028 2301 Fragments arranged by size: 2301 1064 1028 761 275 186 11 BsiHKAI G_wGCw'C Cuts at: 3687 3772 4843 3687 Size: 85 1071 4470 Fragments arranged by size: 4470 1071 85 BsmI GAATG_Cn' Cuts at: 3137 3230 3137 Size: 93 5533 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2204 2945 3687 3772 4843 1391 Size: 399 414 741 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 741 414 399 85 BspEI T'CCGG_A Cuts at: 5289 5289 Size: 5626 BspHI T'CATG_A Cuts at: 3515 5245 3515 Size: 1730 3896 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2981 844 Size: 2137 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2120 3513 1394 Size: 429 297 1393 3507 Fragments arranged by size: 3507 1393 429 297 BsrDI GCAATG_nn' Cuts at: 65 1248 2917 4121 4295 65 Size: 1183 1669 1204 174 1396 Fragments arranged by size: 1669 1396 1204 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4270 4457 1700 Size: 110 2460 187 2869 Fragments arranged by size: 2869 2460 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5626 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5626 BssSI C'ACGA_G Cuts at: 3686 4698 3686 Size: 1012 4614 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5626 BstBI TT'CG_AA Cuts at: 4430 4430 Size: 5626 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5626 BstYI r'GATC_y Cuts at: 1972 2039 2114 2631 2956 3323 3708 3725 5177 Size: 67 75 517 325 367 385 17 1452 Cuts at: 5177 5263 5275 5621 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 517 385 367 346 325 86 75 67 17 12 Bsu36I CC'TnA_GG Cuts at: 2209 2502 2209 Size: 293 5333 BtgI C'CryG_G Cuts at: 513 1065 2222 2398 2811 513 Size: 552 1157 176 413 3328 Fragments arranged by size: 3328 1157 552 413 176 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4887 Fragments arranged by size: 4887 683 56 BtsI GCAGTG_nn' Cuts at: 3142 3936 3956 3142 Size: 794 20 4812 Fragments arranged by size: 4812 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5626 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5591 DraI TTT'AAA Cuts at: 2930 2930 Size: 5626 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4633 809 Size: 507 3317 1802 Fragments arranged by size: 3317 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2141 2401 3073 Size: 54 1425 50 275 18 311 260 672 Cuts at: 3073 3962 5591 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 672 311 275 260 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3073 1537 Size: 275 1261 4090 Fragments arranged by size: 4090 1261 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2708 2721 3556 1353 Size: 1355 13 835 3423 Fragments arranged by size: 3423 1355 835 13 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4213 4587 4733 387 Size: 1064 138 2624 374 146 1280 Fragments arranged by size: 2624 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2886 3689 4286 5073 5341 1239 Size: 393 1254 803 597 787 268 1524 Fragments arranged by size: 1524 1254 803 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2213 2213 Size: 5626 EcoRI G'AATT_C Cuts at: 2333 2935 3313 2333 Size: 602 378 4646 Fragments arranged by size: 4646 602 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2819 2819 Size: 5626 FspI TGC'GCA Cuts at: 4132 4132 Size: 5626 HaeII r_GCGC'y Cuts at: 1678 2036 2053 2171 2239 4773 1678 Size: 358 17 118 68 2534 2531 Fragments arranged by size: 2534 2531 358 118 68 17 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2001 2033 2055 2087 2264 Size: 32 135 32 429 32 22 32 177 Cuts at: 2264 2296 2363 2395 4267 4299 4341 4373 1373 Size: 32 67 32 1872 32 42 32 2626 Fragments arranged by size: 2626 1872 429 177 135 67 42 32 32 32 32 32 32 32 32 22 HincII GTy'rAC Cuts at: 669 1657 1938 2367 2970 3216 669 Size: 988 281 429 603 246 3079 Fragments arranged by size: 3079 988 603 429 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5626 HpaI GTT'AAC Cuts at: 2367 3216 2367 Size: 849 4777 KasI G'GCGC_C Cuts at: 2032 2049 2032 Size: 17 5609 KpnI G_GTAC'C Cuts at: 2951 2951 Size: 5626 MfeI C'AATT_G Cuts at: 3225 3225 Size: 5626 MluI A'CGCG_T Cuts at: 5502 5502 Size: 5626 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2367 2595 4740 4924 1305 Size: 1062 228 2145 184 2007 Fragments arranged by size: 2145 2007 1062 228 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2403 10 Size: 54 1425 343 571 3233 Fragments arranged by size: 3233 1425 571 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2648 2775 3584 3943 4102 518 Size: 648 1482 127 809 359 159 2042 Fragments arranged by size: 2042 1482 809 648 359 159 127 MspA1I CmG'CkG Cuts at: 1606 2146 2224 2746 3042 3719 4867 5112 1606 Size: 540 78 522 296 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 540 522 296 245 78 NarI GG'CG_CC Cuts at: 2033 2050 2033 Size: 17 5609 NcoI C'CATG_G Cuts at: 513 1065 2811 513 Size: 552 1746 3328 Fragments arranged by size: 3328 1746 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5626 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5626 NotI GC'GGCC_GC Cuts at: 3073 3073 Size: 5626 NspI r_CATG'y Cuts at: 1132 1229 2328 2984 4529 1132 Size: 97 1099 656 1545 2229 Fragments arranged by size: 2229 1545 1099 656 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5626 PciI A'CATG_T Cuts at: 4525 4525 Size: 5626 PflMI CCAn_nnn'nTGG Cuts at: 1108 2060 2851 1108 Size: 952 791 3883 Fragments arranged by size: 3883 952 791 PmeI GTTT'AAAC Cuts at: 2930 2930 Size: 5626 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3695 3727 5208 5240 3695 Size: 32 1481 32 4081 Fragments arranged by size: 4081 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2354 1613 Size: 741 4885 PsiI TTA'TAA Cuts at: 3196 3196 Size: 5626 PstI C_TGCA'G Cuts at: 830 2978 830 Size: 2148 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5626 SacII CC_GC'GG Cuts at: 2225 2225 Size: 5626 SalI G'TCGA_C Cuts at: 1936 2968 1936 Size: 1032 4594 SbfI CC_TGCA'GG Cuts at: 2978 2978 Size: 5626 ScaI AGT'ACT Cuts at: 3874 5586 3874 Size: 1712 3914 SfcI C'TryA_G Cuts at: 826 1045 2456 2974 4109 4790 4981 826 Size: 219 1411 518 1135 681 191 1471 Fragments arranged by size: 1471 1411 1135 681 518 219 191 SfoI GGC'GCC Cuts at: 2034 2051 2034 Size: 17 5609 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5626 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5626 SmaI CCC'GGG Cuts at: 1872 2105 2165 2218 2953 1872 Size: 233 60 53 735 4545 Fragments arranged by size: 4545 735 233 60 53 SmlI C'TyrA_G Cuts at: 820 1017 1943 2635 3730 4631 4893 820 Size: 197 926 692 1095 901 262 1553 Fragments arranged by size: 1553 1095 926 901 692 262 197 SnaBI TAC'GTA Cuts at: 493 3417 493 Size: 2924 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5626 SphI G_CATG'C Cuts at: 1132 2984 1132 Size: 1852 3774 SrfI GCCC'GGGC Cuts at: 2165 2165 Size: 5626 SspI AAT'ATT Cuts at: 5 52 2305 2895 3550 5 Size: 47 2253 590 655 2081 Fragments arranged by size: 2253 2081 655 590 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5626 StyI C'CwwG_G Cuts at: 513 1065 2811 3065 513 Size: 552 1746 254 3074 Fragments arranged by size: 3074 1746 552 254 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3653 3670 3823 4008 4453 325 Size: 1255 2073 17 153 185 445 1498 Fragments arranged by size: 2073 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3872 5584 96 Size: 274 80 33 51 1443 1895 1712 138 Fragments arranged by size: 1895 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5586 5618 5586 Size: 32 5594 Tth111I GACn'n_nGTC Cuts at: 1316 2013 1316 Size: 697 4929 XbaI T'CTAG_A Cuts at: 2962 2962 Size: 5626 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5626 XmaI C'CCGG_G Cuts at: 1870 2103 2163 2216 2951 1870 Size: 233 60 53 735 4545 Fragments arranged by size: 4545 735 233 60 53 XmnI GAAnn'nnTTC Cuts at: 2763 3755 2763 Size: 992 4634 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2356 3434 276 Size: 53 83 186 936 822 1078 2468 Fragments arranged by size: 2468 1078 936 822 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SanDI SapI SexAI SfiI SwaI XcmI