################################## # NO CUT ENZYMES ( 147 enzymes) # ################################## Acc65I AccI AccII AccIII AclI AcsI AfaI AflII AgeI AhdI Alw44I AlwNI ApaLI ApoI AscI AseI AsnI Asp718I AspEI AspHI AspI AviII AvrII BalI BbeI BbrPI BcgI BciVI BclI BfaI BfrI BglI BlnI BlpI Bpu1102I BsaAI BsaBI BsaWI BseAI BsiEI BsiHKAI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I CelII Cfr10I ClaI CpoI DrdI EaeI EagI Eam1105I EclXI Eco52I EcoNI EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI KasI KpnI KspI MaeI MamI MfeI MluI MluNI MroI MscI MspA1I MunI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI PvuII RcaI RsaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StyI SwaI Tth111I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 36 enzymes) # ################################### AatII 613 GACGTC GACGT^C AflIII 933 ACATGT A^C[GA][CT]GT AocI 31 CCTGAGG CC^T[ACGT]AGG AvaI 473 CCCGAG C^[CT]CG[GA]G BamHI 332 GGATCC G^GATCC BanI 549 GGTGCC G^G[CT][GA]CC BbvCI 883 CCTCAGC CCTCAGC(-5/-2) BseRI 739 CTCCTC GAGGAG(10/8) BsgI 513 CTGCAC GTGCAG(16/14) BsoBI 473 CCCGAG C^[CT]CG[GA]G BspLU11I 933 ACATGT A^CATGT BssSI 775 CTCGTG CACGAG(-5/-1) BstXI 744 CCAGATCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 31 CCTGAGG CC^T[ACGT]AGG DraI 976 TTTAAA TTT^AAA DraIII 597 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 872 CCACGG C^C[GA][CT]GG EarI 379 CTCTTC CTCTTC(1/4) Eco81I 31 CCTGAGG CC^T[ACGT]AGG HphI 130 GGTGA GGTGA(8/7) Ksp632I 379 CTCTTC CTCTTC(1/4) MaeIII 782 GTGAC ^GT[ACGT]AC MseI 977 TTAA T^TAA MslI 219 CATGTCGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NspI 933 ACATGT [GA]CATG^[CT] PflMI 482 CCAATGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 719 GAGTC GAGTC(4/5) PstI 691 CTGCAG CTGCA^G SfcI 691 CTGCAG C^T[GA][CT]AG SmlI 277 CTCAAG C^T[CT][GA]AG TfiI 360 GATTC G^A[AT]TC Tru9I 977 TTAA T^TAA Tsp45I 782 GTGAC ^GT[GC]AC Tsp509I 982 AATT ^AATT Van91I 482 CCAATGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 659 CCAAAGGCTGCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AcyI 214 GGCGTC G[GA]^CG[CT]C 613 GACGTC AlwI 333 GATCC GGATC(4/5) 332 GGATC Aor51HI 159 AGCGCT AGC^GCT 539 AGCGCT ApaI 251 GGGCCC GGGCC^C 905 GGGCCC Asp700I 51 GAAATACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 264 GAACACCTTC AvaII 573 GGACC G^G[AT]CC 864 GGTCC BglII 168 AGATCT A^GATCT 746 AGATCT BsaHI 214 GGCGTC G[GA]^CG[CT]C 613 GACGTC BsaI 365 GAGACC GGTCTC(1/5) 301 GGTCTC BsmAI 365 GAGAC GTCTC(1/5) 302 GTCTC BsmFI 410 GGGAC GGGAC(10/14) 572 GGGAC BstF5I 651 CATCC GGATG(2/0) 585 GGATG CfoI 160 GCGC GCG^C 540 GCGC Eco47III 159 AGCGCT AGC^GCT 539 AGCGCT FokI 651 CATCC GGATG(9/13) 585 GGATG HaeII 159 AGCGCT [GA]GCGC^[CT] 539 AGCGCT HgaI 215 GCGTC GACGC(5/10) 566 GCGTC HhaI 160 GCGC GCG^C 540 GCGC Hin1I 214 GGCGTC G[GA]^CG[CT]C 613 GACGTC HinP1I 160 GCGC G^CGC 540 GCGC HinfI 360 GATTC G^A[ACGT]TC 719 GAGTC MaeII 395 ACGT A^CGT 614 ACGT PpuMI 572 GGGACCC [GA]G^G[AT]CC[CT] 863 AGGTCCT SfaNI 731 GCATC GCATC(5/9) 755 GCATC StuI 177 AGGCCT AGG^CCT 401 AGGCCT TaqI 148 TCGA T^CGA 363 TCGA TthHB8I 148 TCGA T^CGA 363 TCGA XmnI 51 GAAATACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 264 GAACACCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 47 enzymes) # ######################################### AciI AluI BanII BbsI BbvI BcnI BmyI BpmI BpuAI BsaJI BsiYI BslI Bsp1286I BsrI BssKI BstNI BstYI Cac8I Cfr13I DdeI DpnII DraII EcoO109I EcoRII FauI Fnu4HI HaeIII HapII HpaII ItaI MboI MboII MflI MnlI MspI MvaI MwoI NciI NdeII NlaIII NlaIV Sau3AI Sau96I ScrFI TseI TspRI XhoII