Restriction map for
pFN21AE4203
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# NO CUT ENZYMES ( 103 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AviII AvrII BbrPI BcgI BciVI
BclI BfrI BlnI BsaAI BseAI
BsgI BsiWI BsmI BspDI BspEI
BspHI BspLU11I BssSI Bst1107I BstBI
BstXI BstZ17I ClaI CpoI DraI
Eam1105I EcoNI EcoRI EcoRV EcoT22I
FbaI FseI FspI HincII HindII
HpaI KpnI KspI MfeI MluI
MroI MslI MunI NaeI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI SbfI ScaI SfiI
SfuI SgrAI SnaBI SpeI Sse8387I
SspI SwaI Tsp509I Tth111I XbaI
XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AcyI 856 GGCGCC G[GA]^CG[CT]C
Aor51HI 995 AGCGCT AGC^GCT
BalI 290 TGGCCA TGG^CCA
BbeI 856 GGCGCC GGCGC^C
BglII 399 AGATCT A^GATCT
BlpI 595 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 595 GCTGAGC GC^T[ACGT]AGC
BsaBI 981 GATCCGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 856 GGCGCC G[GA]^CG[CT]C
BsaWI 299 ACCGGA [AT]^CCGG[AT]
BsiEI 841 CGGCCG CG[GA][CT]^CG
BsmBI 1570 CGTCTC CGTCTC(1/5)
Bsp1407I 77 TGTACA T^GTACA
BspMI 1763 ACCTGC ACCTGC(4/8)
BsrBI 223 GAGCGG CCGCTC(-3/-3)
BsrDI 159 CATTGC GCAATG(2/0)
BsrGI 77 TGTACA T^GTACA
BstAPI 1950 GCACAATGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1661 GGTCACC G^GT[ACGT]ACC
BstPI 1661 GGTCACC G^GT[ACGT]ACC
CelII 595 GCTGAGC GC^T[ACGT]AGC
DrdI 481 GACCCCCCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 841 CGGCCG C^GGCCG
EclXI 841 CGGCCG C^GGCCG
Eco47III 995 AGCGCT AGC^GCT
Eco52I 841 CGGCCG C^GGCCG
EcoO65I 1661 GGTCACC G^GT[ACGT]ACC
Hin1I 856 GGCGCC G[GA]^CG[CT]C
KasI 856 GGCGCC G^GCGCC
MaeII 2039 ACGT A^CGT
MamI 981 GATCCGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 290 TGGCCA TGG^CCA
MscI 290 TGGCCA TGG^CCA
MseI 1340 TTAA T^TAA
NarI 856 GGCGCC GG^CGCC
NspI 1452 GCATGC [GA]CATG^[CT]
PflMI 723 CCAGCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SapI 783 GCTCTTC GCTCTTC(1/4)
SexAI 1289 ACCTGGT A^CC[AT]GGT
SfoI 856 GGCGCC GGC^GCC
SphI 1452 GCATGC GCATG^C
SspBI 77 TGTACA T^GTACA
StuI 680 AGGCCT AGG^CCT
Tru9I 1340 TTAA T^TAA
Van91I 723 CCAGCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 12 enzymes) #
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BamHI 980 GGATCC G^GATCC
1201 GGATCC
BbsI 384 GAAGAC GAAGAC(2/6)
2041 GTCTTC
BfaI 1610 CTAG C^TAG
1679 CTAG
BpuAI 384 GAAGAC GAAGAC(2/6)
2041 GTCTTC
DraIII 1607 CACCTAGTG CAC[ACGT][ACGT][ACGT]^GTG
1951 CACAATGTG
DsaI 881 CCGTGG C^C[GA][CT]GG
1872 CCACGG
EcoT14I 791 CCAAGG C^C[AT][AT]GG
1269 CCTTGG
HgaI 1569 GCGTC GACGC(5/10)
1853 GCGTC
HindIII 568 AAGCTT A^AGCTT
1582 AAGCTT
MaeI 1610 CTAG C^TAG
1679 CTAG
SacI 781 GAGCTC GAGCT^C
1830 GAGCTC
StyI 791 CCAAGG C^C[AT][AT]GG
1269 CCTTGG
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# 3 OR MORE CUT ENZYMES ( 98 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI AocI ApaI AspHI AvaI
AvaII BanI BanII BbvCI BbvI
BcnI BglI BmyI BpmI BsaI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BsrFI
BsrI BssHII BssKI BstF5I BstNI
BstUI BstYI Bsu36I Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
EaeI EarI Eco81I EcoO109I EcoRII
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeIII MboI
MboII MflI MnlI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV PleI PpuMI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmaI SmlI TaqI
TfiI TseI Tsp45I TspRI TthHB8I
XhoII XmaCI XmaI