Restriction map for
pFN21AE4203
(Circular) (Six-base) MAPSORT of: pFN21AE4203.seq Check: 2408 from: 1 to: 6835
pFN21AE4203 6835 bp
With 182 enzymes: SgfI *
March 28, 2012 13:15 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4645 278
Size: 53 83 186 936 3109 2468
Fragments arranged by size:
3109 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4178 1937
Size: 2241 4594
Acc65I G'GTAC_C
Cuts at: 4156 4156
Size: 6835
AclI AA'CG_TT
Cuts at: 1563 4962 5335 1563
Size: 3399 373 3063
Fragments arranged by size:
3399 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2664 2725 4898 6282 6550 1632
Size: 1032 61 2173 1384 268 1917
Fragments arranged by size:
2173 1917 1384 1032 268 61
AfeI AGC'GCT
Cuts at: 3002 3002
Size: 6835
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6638
AflIII A'CryG_T
Cuts at: 1203 5734 6711 1203
Size: 4531 977 1327
Fragments arranged by size:
4531 1327 977
AgeI A'CCGG_T
Cuts at: 5666 5666
Size: 6835
AhdI GACnn_n'nnGTC
Cuts at: 5564 5564
Size: 6835
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6835
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3767 1184
Size: 2583 4252
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3011 3043 3011
Size: 32 6803
AlwNI CAG_nnn'CTG
Cuts at: 2734 2974 3263 3397 4578 6150 2734
Size: 240 289 134 1181 1572 3419
Fragments arranged by size:
3419 1572 1181 289 240 134
ApaI G_GGCC'C
Cuts at: 2138 3732 3740 2138
Size: 1594 8 5233
Fragments arranged by size:
5233 1594 8
ApaLI G'TGCA_C
Cuts at: 4892 6048 4892
Size: 1156 5679
ApoI r'AATT_y
Cuts at: 1493 4144 4370 4522 6631 1493
Size: 2651 226 152 2109 1697
Fragments arranged by size:
2651 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5389 160
Size: 872 4357 1606
Fragments arranged by size:
4357 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6835
AvaI C'yCGr_G
Cuts at: 1870 1943 2138 2372 3501 3594 3626 4160 1870
Size: 73 195 234 1129 93 32 534 4545
Fragments arranged by size:
4545 1129 534 234 195 93 73 32
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2298 2331 2298
Size: 33 6802
BamHI G'GATC_C
Cuts at: 2985 3206 4165 4532 2985
Size: 221 959 367 5288
Fragments arranged by size:
5288 959 367 221
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2038 2861 3278 4156 5611
Size: 325 217 627 251 823 417 878 1455
Cuts at: 5611 618
Size: 1842
Fragments arranged by size:
1842 1455 878 823 627 417 325 251
217
BanII G_rGCy'C
Cuts at: 1391 2126 2138 2453 2712 2790 2825 3431 3732
Size: 735 12 315 259 78 35 606 301
Cuts at: 3732 3740 3839 4154 1391
Size: 8 99 315 4072
Fragments arranged by size:
4072 735 606 315 315 301 259 99
78 35 12 8
BbeI G_GCGC'C
Cuts at: 2865 2865
Size: 6835
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2396 4039 928
Size: 971 497 1643 3724
Fragments arranged by size:
3724 1643 971 497
BbvCI CC'TCA_GC
Cuts at: 2210 2553 3164 2210
Size: 343 611 5881
Fragments arranged by size:
5881 611 343
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5026 5060 1903
Size: 34 3089 34 3678
Fragments arranged by size:
3678 3089 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4727 5937 4727
Size: 1210 5625
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6835
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2142 2294 2334
Size: 107 122 71 631 605 470 152 40
Cuts at: 2334 2888 5446 136
Size: 554 2558 1525
Fragments arranged by size:
2558 1525 631 605 554 470 152 122
107 71 40
BglII A'GATC_T
Cuts at: 2404 6830 2404
Size: 4426 2409
BlpI GC'TnA_GC
Cuts at: 2601 4252 2601
Size: 1651 5184
Bme1580I G_kGCm'C
Cuts at: 1790 2138 3732 3740 3966 4896 6052 1790
Size: 348 1594 8 226 930 1156 2573
Fragments arranged by size:
2573 1594 1156 930 348 226 8
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6835
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2062 2887 4566
Size: 416 53 83 399 24 645 825 1679
Cuts at: 4566 5524 442
Size: 958 1753
Fragments arranged by size:
1753 1679 958 825 645 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6835
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2809 3622 2809
Size: 813 6022
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2246 2996 3634 3708 5495 1239
Size: 1007 750 638 74 1787 2579
Fragments arranged by size:
2579 1787 1007 750 638 74
Bpu10I CC'TnA_GC
Cuts at: 2210 2553 3164 3425 3758 2210
Size: 343 611 261 333 5287
Fragments arranged by size:
5287 611 343 333 261
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2703 3062 3661 4960 5825 6123 2703
Size: 359 599 1299 865 298 3415
Fragments arranged by size:
3415 1299 865 599 359 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2057 2250 2478 5498 882
Size: 617 558 193 228 3020 2219
Fragments arranged by size:
3020 2219 617 558 228 193
BsaAI yAC'GTr
Cuts at: 493 1204 4626 493
Size: 711 3422 2702
Fragments arranged by size:
3422 2702 711
BsaBI GATnn'nnATC
Cuts at: 2990 6829 2990
Size: 3839 2996
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2862 4642 5024 5659
Size: 53 83 186 936 1329 1780 382 635
Cuts at: 5659 275
Size: 1451
Fragments arranged by size:
1780 1451 1329 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2304 5268 5666 5940 6087 6498 2304
Size: 2964 398 274 147 411 2641
Fragments arranged by size:
2964 2641 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2075 2105 2232 2262 2629
Size: 30 165 30 672 30 127 30 367
Cuts at: 2629 2659 3011 3041 3482 3512 1178
Size: 30 352 30 441 30 4501
Fragments arranged by size:
4501 672 441 367 352 165 127 30
30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2609 2633 3016 3206 3373 3494 1186
Size: 1423 24 383 190 167 121 4527
Fragments arranged by size:
4527 1423 383 190 167 121 24
BseYI C'CCAG_C
Cuts at: 2156 2273 2332 2446 2532 2727 2920 2997 3087
Size: 117 59 114 86 195 193 77 90
Cuts at: 3087 3533 3740 3773 3926 3966 6038 6534 2156
Size: 446 207 33 153 40 2072 496 2457
Fragments arranged by size:
2457 2072 496 446 207 195 193 153
117 114 90 86 77 59 40 33
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6262
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2849 4285 5046 6074 1540
Size: 275 186 848 1436 761 1028 2301
Fragments arranged by size:
2301 1436 1028 848 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2790 2934 2941 3839 4023 4896 4981 6052 2790
Size: 144 7 898 184 873 85 1071 3573
Fragments arranged by size:
3573 1071 898 873 184 144 85 7
BsmI GAATG_Cn'
Cuts at: 4346 4439 4346
Size: 93 6742
BsmBI CGTCTCn'nnnn_
Cuts at: 3581 3581
Size: 6835
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2126 2138 2453 2712 2790 2825 2934
Size: 399 336 12 315 259 78 35 109
Cuts at: 2934 2941 3431 3732 3740 3839 3966 4023 4154
Size: 7 490 301 8 99 127 57 131
Cuts at: 4154 4896 4981 6052 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 490 399 336 315 301
259 131 127 109 99 85 78 57
35 12 8 7
BspEI T'CCGG_A
Cuts at: 6498 6498
Size: 6835
BspHI T'CATG_A
Cuts at: 4724 6454 4724
Size: 1730 5105
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3777 4190 844
Size: 2933 413 3489
Fragments arranged by size:
3489 2933 413
BsrBI CCG'CTC
Cuts at: 1394 1823 2230 4722 1394
Size: 429 407 2492 3507
Fragments arranged by size:
3507 2492 429 407
BsrDI GCAATG_nn'
Cuts at: 65 1248 2163 5330 5504 65
Size: 1183 915 3167 174 1396
Fragments arranged by size:
3167 1396 1183 915 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2844 2909 3072 3492 5479 5666 1700
Size: 110 1034 65 163 420 1987 187 2869
Fragments arranged by size:
2869 1987 1034 420 187 163 110 65
BsrGI T'GTAC_A
Cuts at: 96 2082 96
Size: 1986 4849
BssHII G'CGCG_C
Cuts at: 1925 2219 2878 3034 1925
Size: 294 659 156 5726
Fragments arranged by size:
5726 659 294 156
BssSI C'ACGA_G
Cuts at: 4895 5907 4895
Size: 1012 5823
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3961 794
Size: 3167 3668
BstBI TT'CG_AA
Cuts at: 5639 5639
Size: 6835
BstEII G'GTnAC_C
Cuts at: 1184 3666 1184
Size: 2482 4353
BstYI r'GATC_y
Cuts at: 1972 2404 2985 3206 3337 3375 4165 4532 4917
Size: 432 581 221 131 38 790 367 385
Cuts at: 4917 4934 6386 6472 6484 6830 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 790 581 432 385 367 346
221 131 86 38 17 12
Bsu36I CC'TnA_GG
Cuts at: 2426 2472 2628 2426
Size: 46 156 6633
Fragments arranged by size:
6633 156 46
BtgI C'CryG_G
Cuts at: 513 1065 2004 2886 3877 513
Size: 552 939 882 991 3471
Fragments arranged by size:
3471 991 939 882 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2977 487
Size: 683 56 1751 4345
Fragments arranged by size:
4345 1751 683 56
BtsI GCAGTG_nn'
Cuts at: 2319 2518 4351 5145 5165 2319
Size: 199 1833 794 20 3989
Fragments arranged by size:
3989 1833 794 199 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6835
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6800
DraI TTT'AAA
Cuts at: 4139 4139
Size: 6835
DraIII CAC_nnn'GTG
Cuts at: 3617 3961 3617
Size: 344 6491
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2492 5842 809
Size: 507 1176 3350 1802
Fragments arranged by size:
3350 1802 1176 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2295 2757 2846
Size: 54 1425 50 275 18 465 462 89
Cuts at: 2846 2949 4282 5171 6800 8
Size: 103 1333 889 1629 43
Fragments arranged by size:
1629 1425 1333 889 465 462 275 103
89 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2846 4282 1537
Size: 275 1034 1436 4090
Fragments arranged by size:
4090 1436 1034 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2055 2610 2683 2694 2795 3210 3350 4765
Size: 702 555 73 11 101 415 140 1415
Cuts at: 4765 1353
Size: 3423
Fragments arranged by size:
3423 1415 702 555 415 140 101 73
11
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2144 3037 3571 4108 5422 5796
Size: 1064 138 555 893 534 537 1314 374
Cuts at: 5796 5942 387
Size: 146 1280
Fragments arranged by size:
1314 1280 1064 893 555 537 534 374
146 138
EcoICRI GAG'CTC
Cuts at: 2788 3837 2788
Size: 1049 5786
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2246 2664 2725 2996 3634 3708 4898
Size: 393 614 418 61 271 638 74 1190
Cuts at: 4898 5495 6282 6550 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1190 787 638 614 597 418 393
271 268 74 61
EcoO109I rG'GnC_Cy
Cuts at: 2134 2135 2269 2642 2723 3260 3631 3728 3729
Size: 1 134 373 81 537 371 97 1
Cuts at: 3729 3736 3737 4104 2134
Size: 7 1 367 4865
Fragments arranged by size:
4865 537 373 371 367 134 97 81
7 1 1 1
EcoRI G'AATT_C
Cuts at: 4144 4522 4144
Size: 378 6457
FspI TGC'GCA
Cuts at: 5341 5341
Size: 6835
HaeII r_GCGC'y
Cuts at: 1678 2865 3004 3409 5982 1678
Size: 1187 139 405 2573 2531
Fragments arranged by size:
2573 2531 1187 405 139
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2629 2660 2661 2692 3394
Size: 32 135 32 1057 31 1 31 702
Cuts at: 3394 3426 3543 3575 3623 3655 5476 5508 5550
Size: 32 117 32 48 32 1821 32 42
Cuts at: 5550 5582 1373
Size: 32 2626
Fragments arranged by size:
2626 1821 1057 702 135 117 48 42
32 32 32 32 32 32 32 31
31 1
HincII GTy'rAC
Cuts at: 669 1657 1938 4179 4425 669
Size: 988 281 2241 246 3079
Fragments arranged by size:
3079 2241 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2573 3587 748
Size: 1825 1014 3996
Fragments arranged by size:
3996 1825 1014
HpaI GTT'AAC
Cuts at: 4425 4425
Size: 6835
KasI G'GCGC_C
Cuts at: 2861 2861
Size: 6835
KpnI G_GTAC'C
Cuts at: 4160 4160
Size: 6835
MfeI C'AATT_G
Cuts at: 4434 4434
Size: 6835
MluI A'CGCG_T
Cuts at: 6711 6711
Size: 6835
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3309 3574 5949 6133 1305
Size: 2004 265 2375 184 2007
Fragments arranged by size:
2375 2007 2004 265 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2297 10
Size: 54 1425 343 465 4548
Fragments arranged by size:
4548 1425 465 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4793 5152 5311 518
Size: 648 3627 359 159 2042
Fragments arranged by size:
3627 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2044 2396 2767 2971 3087 3397 3521 4251
Size: 438 352 371 204 116 310 124 730
Cuts at: 4251 4928 6076 6321 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 730 677 438 371 352 310
245 204 124 116
NarI GG'CG_CC
Cuts at: 2862 2862
Size: 6835
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5344
Fragments arranged by size:
5344 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6835
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6835
NotI GC'GGCC_GC
Cuts at: 4282 4282
Size: 6835
NspI r_CATG'y
Cuts at: 1132 1229 3461 4193 5738 1132
Size: 97 2232 732 1545 2229
Fragments arranged by size:
2232 2229 1545 732 97
PasI CC'CwG_GG
Cuts at: 1348 2543 3662 3733 3972 1348
Size: 1195 1119 71 239 4211
Fragments arranged by size:
4211 1195 1119 239 71
PciI A'CATG_T
Cuts at: 5734 5734
Size: 6835
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2734 1108
Size: 1626 5209
PfoI T'CCnGG_A
Cuts at: 2733 3186 2733
Size: 453 6382
PmeI GTTT'AAAC
Cuts at: 4139 4139
Size: 6835
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3011 3043 4904 4936 6417 6449 3011
Size: 32 1861 32 1481 32 3397
Fragments arranged by size:
3397 1861 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2269 2642 2723 3631 4104 2269
Size: 373 81 908 473 5000
Fragments arranged by size:
5000 908 473 373 81
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6835
PsiI TTA'TAA
Cuts at: 4405 4405
Size: 6835
PspOMI G'GGCC_C
Cuts at: 2134 3728 3736 2134
Size: 1594 8 5233
Fragments arranged by size:
5233 1594 8
PstI C_TGCA'G
Cuts at: 830 2049 2463 2860 3402 4187 830
Size: 1219 414 397 542 785 3478
Fragments arranged by size:
3478 1219 785 542 414 397
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6835
PvuII CAG'CTG
Cuts at: 2767 3087 3397 3521 2767
Size: 320 310 124 6081
Fragments arranged by size:
6081 320 310 124
SacI G_AGCT'C
Cuts at: 2790 3839 2790
Size: 1049 5786
SalI G'TCGA_C
Cuts at: 1936 4177 1936
Size: 2241 4594
SanDI GG'GwC_CC
Cuts at: 3631 4104 3631
Size: 473 6362
SapI GCTCTTCn'nnn_
Cuts at: 2795 2795
Size: 6835
SbfI CC_TGCA'GG
Cuts at: 4187 4187
Size: 6835
ScaI AGT'ACT
Cuts at: 5083 6795 5083
Size: 1712 5123
SexAI A'CCwGG_T
Cuts at: 3294 3294
Size: 6835
SfcI C'TryA_G
Cuts at: 826 1045 2045 2459 2856 3398 4183 5318 5999
Size: 219 1000 414 397 542 785 1135 681
Cuts at: 5999 6190 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1000 785 681 542 414 397
219 191
SfoI GGC'GCC
Cuts at: 2863 2863
Size: 6835
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6835
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6835
SmaI CCC'GGG
Cuts at: 1872 2140 2374 3503 3628 4162 1872
Size: 268 234 1129 125 534 4545
Fragments arranged by size:
4545 1129 534 268 234 125
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2718 3077 3676 4939 5840 6102
Size: 197 926 775 359 599 1263 901 262
Cuts at: 6102 820
Size: 1553
Fragments arranged by size:
1553 1263 926 901 775 599 359 262
197
SnaBI TAC'GTA
Cuts at: 493 4626 493
Size: 4133 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6835
SphI G_CATG'C
Cuts at: 1132 3461 4193 1132
Size: 2329 732 3774
Fragments arranged by size:
3774 2329 732
SspI AAT'ATT
Cuts at: 5 52 4759 5
Size: 47 4707 2081
Fragments arranged by size:
4707 2081 47
StuI AGG'CCT
Cuts at: 1517 2687 1517
Size: 1170 5665
StyI C'CwwG_G
Cuts at: 513 1065 2004 2796 3274 4274 513
Size: 552 939 792 478 1000 3074
Fragments arranged by size:
3074 1000 939 792 552 478
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2081 4862 4879 5032 5217 5662 325
Size: 1255 501 2781 17 153 185 445 1498
Fragments arranged by size:
2781 1498 1255 501 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2082 5081 6793
Size: 274 80 33 51 1443 105 2999 1712
Cuts at: 6793 96
Size: 138
Fragments arranged by size:
2999 1712 1443 274 138 105 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2232 2264 6795 6827 2232
Size: 32 4531 32 2240
Fragments arranged by size:
4531 2240 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6835
XbaI T'CTAG_A
Cuts at: 4171 4171
Size: 6835
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6835
XmaI C'CCGG_G
Cuts at: 1870 2138 2372 3501 3626 4160 1870
Size: 268 234 1129 125 534 4545
Fragments arranged by size:
4545 1129 534 268 234 125
XmnI GAAnn'nnTTC
Cuts at: 4964 4964
Size: 6835
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4643 276
Size: 53 83 186 936 3109 2468
Fragments arranged by size:
3109 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NarI NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI
SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AscI AvrII BfrBI BsiWI BstXI BstZ17I EcoNI
EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI
PacI PmlI PspXI PsrI RsrII SacII SfiI SrfI
SwaI XcmI