(Linear) (Six-base) MAPSORT of: KIEE4203.seq Check: 6361 from: 1 to: 2130 KIEE4203.orf With 182 enzymes: SgfI * March 22, 2012 17:34 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 659 720 2130 Size: 659 61 1410 Fragments arranged by size: 1410 659 61 AfeI AGC'GCT Cuts at: 0 997 2130 Size: 997 1133 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1762 2130 Size: 1762 368 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1006 1038 2130 Size: 1006 32 1092 Fragments arranged by size: 1092 1006 32 AlwNI CAG_nnn'CTG Cuts at: 0 729 969 1258 1392 2130 Size: 729 240 289 134 738 Fragments arranged by size: 738 729 289 240 134 ApaI G_GGCC'C Cuts at: 0 133 1727 1735 2130 Size: 133 1594 8 395 Fragments arranged by size: 1594 395 133 8 AvaI C'yCGr_G Cuts at: 0 133 367 1496 1589 1621 2130 Size: 133 234 1129 93 32 509 Fragments arranged by size: 1129 509 234 133 93 32 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 293 326 2130 Size: 293 33 1804 Fragments arranged by size: 1804 293 33 BamHI G'GATC_C Cuts at: 0 980 1201 2130 Size: 980 221 929 Fragments arranged by size: 980 929 221 BanI G'GyrC_C Cuts at: 0 33 856 1273 2130 Size: 33 823 417 857 Fragments arranged by size: 857 823 417 33 BanII G_rGCy'C Cuts at: 0 121 133 448 707 785 820 1426 1727 Size: 121 12 315 259 78 35 606 301 Cuts at: 1727 1735 1834 2130 Size: 8 99 296 Fragments arranged by size: 606 315 301 296 259 121 99 78 35 12 8 BbeI G_GCGC'C Cuts at: 0 860 2130 Size: 860 1270 BbsI GAAGACnn'nnnn_ Cuts at: 0 391 2034 2130 Size: 391 1643 96 Fragments arranged by size: 1643 391 96 BbvCI CC'TCA_GC Cuts at: 0 205 548 1159 2130 Size: 205 343 611 971 Fragments arranged by size: 971 611 343 205 BglI GCCn_nnn'nGGC Cuts at: 0 137 289 329 883 2130 Size: 137 152 40 554 1247 Fragments arranged by size: 1247 554 152 137 40 BglII A'GATC_T Cuts at: 0 399 2130 Size: 399 1731 BlpI GC'TnA_GC Cuts at: 0 596 2130 Size: 596 1534 Bme1580I G_kGCm'C Cuts at: 0 133 1727 1735 1961 2130 Size: 133 1594 8 226 169 Fragments arranged by size: 1594 226 169 133 8 BmrI ACTGGGnnnn_n' Cuts at: 0 57 882 2130 Size: 57 825 1248 Fragments arranged by size: 1248 825 57 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 804 1617 2130 Size: 804 813 513 Fragments arranged by size: 813 804 513 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 241 991 1629 1703 2130 Size: 241 750 638 74 427 Fragments arranged by size: 750 638 427 241 74 Bpu10I CC'TnA_GC Cuts at: 0 205 548 1159 1420 1753 2130 Size: 205 343 611 261 333 377 Fragments arranged by size: 611 377 343 333 261 205 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 698 1057 1656 2130 Size: 698 359 599 474 Fragments arranged by size: 698 599 474 359 BsaI GGTCTCn'nnnn_ Cuts at: 0 52 245 473 2130 Size: 52 193 228 1657 Fragments arranged by size: 1657 228 193 52 BsaBI GATnn'nnATC Cuts at: 0 985 2130 Size: 985 1145 BsaHI Gr'CG_yC Cuts at: 0 857 2130 Size: 857 1273 BsaWI w'CCGG_w Cuts at: 0 299 2130 Size: 299 1831 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 70 100 227 257 624 654 1006 1036 Size: 70 30 127 30 367 30 352 30 Cuts at: 1036 1477 1507 2130 Size: 441 30 623 Fragments arranged by size: 623 441 367 352 127 70 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 604 628 1011 1201 1368 1489 2130 Size: 604 24 383 190 167 121 641 Fragments arranged by size: 641 604 383 190 167 121 24 BseYI C'CCAG_C Cuts at: 0 151 268 327 441 527 722 915 992 Size: 151 117 59 114 86 195 193 77 Cuts at: 992 1082 1528 1735 1768 1921 1961 2130 Size: 90 446 207 33 153 40 169 Fragments arranged by size: 446 207 195 193 169 153 151 117 114 90 86 77 59 40 33 BsiEI CG_ry'CG Cuts at: 0 844 2130 Size: 844 1286 BsiHKAI G_wGCw'C Cuts at: 0 785 929 936 1834 2018 2130 Size: 785 144 7 898 184 112 Fragments arranged by size: 898 785 184 144 112 7 BsmBI CGTCTCn'nnnn_ Cuts at: 0 1576 2130 Size: 1576 554 Bsp1286I G_dGCh'C Cuts at: 0 121 133 448 707 785 820 929 936 Size: 121 12 315 259 78 35 109 7 Cuts at: 936 1426 1727 1735 1834 1961 2018 2130 Size: 490 301 8 99 127 57 112 Fragments arranged by size: 490 315 301 259 127 121 112 109 99 78 57 35 12 8 7 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1772 2130 Size: 1772 358 BsrBI CCG'CTC Cuts at: 0 225 2130 Size: 225 1905 BsrDI GCAATG_nn' Cuts at: 0 158 2130 Size: 158 1972 BsrFI r'CCGG_y Cuts at: 0 839 904 1067 1487 2130 Size: 839 65 163 420 643 Fragments arranged by size: 839 643 420 163 65 BsrGI T'GTAC_A Cuts at: 0 77 2130 Size: 77 2053 BssHII G'CGCG_C Cuts at: 0 214 873 1029 2130 Size: 214 659 156 1101 Fragments arranged by size: 1101 659 214 156 BstAPI GCAn_nnn'nTGC Cuts at: 0 1956 2130 Size: 1956 174 BstEII G'GTnAC_C Cuts at: 0 1661 2130 Size: 1661 469 BstYI r'GATC_y Cuts at: 0 399 980 1201 1332 1370 2130 Size: 399 581 221 131 38 760 Fragments arranged by size: 760 581 399 221 131 38 Bsu36I CC'TnA_GG Cuts at: 0 421 467 623 2130 Size: 421 46 156 1507 Fragments arranged by size: 1507 421 156 46 BtgI C'CryG_G Cuts at: 0 881 1872 2130 Size: 881 991 258 Fragments arranged by size: 991 881 258 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 972 2130 Size: 972 1158 BtsI GCAGTG_nn' Cuts at: 0 314 513 2130 Size: 314 199 1617 Fragments arranged by size: 1617 314 199 DraIII CAC_nnn'GTG Cuts at: 0 1612 1956 2130 Size: 1612 344 174 Fragments arranged by size: 1612 344 174 DrdI GACnn_nn'nnGTC Cuts at: 0 487 2130 Size: 487 1643 EaeI y'GGCC_r Cuts at: 0 290 752 841 944 2130 Size: 290 462 89 103 1186 Fragments arranged by size: 1186 462 290 103 89 EagI C'GGCC_G Cuts at: 0 841 2130 Size: 841 1289 EarI CTCTTCn'nnn_ Cuts at: 0 50 605 678 689 790 1205 1345 2130 Size: 50 555 73 11 101 415 140 785 Fragments arranged by size: 785 555 415 140 101 73 50 11 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 139 1032 1566 2103 2130 Size: 139 893 534 537 27 Fragments arranged by size: 893 537 534 139 27 EcoICRI GAG'CTC Cuts at: 0 783 1832 2130 Size: 783 1049 298 Fragments arranged by size: 1049 783 298 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 241 659 720 991 1629 1703 2130 Size: 241 418 61 271 638 74 427 Fragments arranged by size: 638 427 418 271 241 74 61 EcoO109I rG'GnC_Cy Cuts at: 0 129 130 264 637 718 1255 1626 1723 Size: 129 1 134 373 81 537 371 97 Cuts at: 1723 1724 1731 1732 2099 2130 Size: 1 7 1 367 31 Fragments arranged by size: 537 373 371 367 134 129 97 81 31 7 1 1 1 HaeII r_GCGC'y Cuts at: 0 860 999 1404 2130 Size: 860 139 405 726 Fragments arranged by size: 860 726 405 139 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 624 655 656 687 1389 1421 1538 1570 Size: 624 31 1 31 702 32 117 32 Cuts at: 1570 1618 1650 2130 Size: 48 32 480 Fragments arranged by size: 702 624 480 117 48 32 32 32 31 31 1 HindIII A'AGCT_T Cuts at: 0 568 1582 2130 Size: 568 1014 548 Fragments arranged by size: 1014 568 548 KasI G'GCGC_C Cuts at: 0 856 2130 Size: 856 1274 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1304 1569 2130 Size: 1304 265 561 Fragments arranged by size: 1304 561 265 MscI TGG'CCA Cuts at: 0 292 2130 Size: 292 1838 MspA1I CmG'CkG Cuts at: 0 39 391 762 966 1082 1392 1516 2130 Size: 39 352 371 204 116 310 124 614 Fragments arranged by size: 614 371 352 310 204 124 116 39 NarI GG'CG_CC Cuts at: 0 857 2130 Size: 857 1273 NspI r_CATG'y Cuts at: 0 1456 2130 Size: 1456 674 PasI CC'CwG_GG Cuts at: 0 538 1657 1728 1967 2130 Size: 538 1119 71 239 163 Fragments arranged by size: 1119 538 239 163 71 PflMI CCAn_nnn'nTGG Cuts at: 0 729 2130 Size: 729 1401 PfoI T'CCnGG_A Cuts at: 0 728 1181 2130 Size: 728 453 949 Fragments arranged by size: 949 728 453 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1006 1038 2130 Size: 1006 32 1092 Fragments arranged by size: 1092 1006 32 PpuMI rG'GwC_Cy Cuts at: 0 264 637 718 1626 2099 2130 Size: 264 373 81 908 473 31 Fragments arranged by size: 908 473 373 264 81 31 PspOMI G'GGCC_C Cuts at: 0 129 1723 1731 2130 Size: 129 1594 8 399 Fragments arranged by size: 1594 399 129 8 PstI C_TGCA'G Cuts at: 0 44 458 855 1397 2130 Size: 44 414 397 542 733 Fragments arranged by size: 733 542 414 397 44 PvuII CAG'CTG Cuts at: 0 762 1082 1392 1516 2130 Size: 762 320 310 124 614 Fragments arranged by size: 762 614 320 310 124 SacI G_AGCT'C Cuts at: 0 785 1834 2130 Size: 785 1049 296 Fragments arranged by size: 1049 785 296 SanDI GG'GwC_CC Cuts at: 0 1626 2099 2130 Size: 1626 473 31 Fragments arranged by size: 1626 473 31 SapI GCTCTTCn'nnn_ Cuts at: 0 790 2130 Size: 790 1340 SexAI A'CCwGG_T Cuts at: 0 1289 2130 Size: 1289 841 SfcI C'TryA_G Cuts at: 0 40 454 851 1393 2130 Size: 40 414 397 542 737 Fragments arranged by size: 737 542 414 397 40 SfoI GGC'GCC Cuts at: 0 858 2130 Size: 858 1272 SmaI CCC'GGG Cuts at: 0 135 369 1498 1623 2130 Size: 135 234 1129 125 507 Fragments arranged by size: 1129 507 234 135 125 SmlI C'TyrA_G Cuts at: 0 713 1072 1671 2130 Size: 713 359 599 459 Fragments arranged by size: 713 599 459 359 SphI G_CATG'C Cuts at: 0 1456 2130 Size: 1456 674 StuI AGG'CCT Cuts at: 0 682 2130 Size: 682 1448 StyI C'CwwG_G Cuts at: 0 791 1269 2130 Size: 791 478 861 Fragments arranged by size: 861 791 478 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 76 2130 Size: 76 2054 TatI w'GTAC_w Cuts at: 0 77 2130 Size: 77 2053 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 227 259 2130 Size: 227 32 1871 Fragments arranged by size: 1871 227 32 XmaI C'CCGG_G Cuts at: 0 133 367 1496 1621 2130 Size: 133 234 1129 125 509 Fragments arranged by size: 1129 509 234 133 125 Enzymes that do cut: AcuI AfeI AlfI AloI AlwNI ApaI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BstAPI BstEII BstYI Bsu36I BtgI BtgZI BtsI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I HaeII Hin4I HindIII KasI MmeI MscI MspA1I NarI NspI PasI PflMI PfoI PpiI PpuMI PspOMI PstI PvuII SacI SanDI SapI SexAI SfcI SfoI SmaI SmlI SphI StuI StyI TaqII TatI TstI XmaI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AflII AflIII AgeI AhdI AleI ApaLI ApoI AscI AseI AsiSI AvrII BcgI BciVI BclI BfrBI BmgBI BmtI BsaAI BsgI BsiWI BsmI BspEI BspHI BssSI BstBI BstXI BstZ17I ClaI CspCI DraI EcoNI EcoRI EcoRV FalI FseI FspI FspAI HincII HpaI KpnI MfeI MluI MslI NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI PciI PmeI PmlI PshAI PsiI PspXI PsrI PvuI RsrII SacII SalI SbfI ScaI SfiI SgfI SgrAI SnaBI SpeI SrfI SspI SwaI Tth111I XbaI XcmI XhoI XmnI ZraI