################################## # NO CUT ENZYMES ( 145 enzymes) # ################################## Acc65I AccI AccII AclI AcsI AflII AgeI AhdI Alw44I Aor51HI ApaLI ApoI AscI AseI AsnI Asp718I AspEI AspI AvaI AviII AvrII BbeI BbrPI BbsI BbvCI BcgI BciVI BfaI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BpuAI BsaBI BsaI BsiEI BsiWI BsmAI BsmBI BsmI BsoBI Bsp1407I BspDI BspHI BspLU11I BspMI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I CelII ClaI CpoI DraI DraIII DsaI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO65I EcoRI EcoRV EcoT22I FseI FspI HaeII HgaI HincII HindII HindIII HpaI KasI KpnI KspI MaeI MamI MluI MseI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tru9I Tth111I Van91I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### AatII 997 GACGTC GACGT^C AccIII 380 TCCGGA T^CCGGA AcyI 997 GACGTC G[GA]^CG[CT]C AfaI 336 GTAC GT^AC AflIII 332 ACACGT A^C[GA][CT]GT AocI 219 CCTGAGG CC^T[ACGT]AGG ApaI 706 GGGCCC GGGCC^C Asp700I 466 GAAACCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BalI 188 TGGCCA TGG^CCA BamHI 638 GGATCC G^GATCC BanI 122 GGTGCC G^G[CT][GA]CC BclI 230 TGATCA T^GATCA BsaAI 333 CACGTA [CT]AC^GT[GA] BsaHI 997 GACGTC G[GA]^CG[CT]C BseAI 380 TCCGGA T^CCGGA BsgI 10 CTGCAC GTGCAG(16/14) BspEI 380 TCCGGA T^CCGGA BsrBI 634 GAGCGG CCGCTC(-3/-3) BsrDI 246 CATTGC GCAATG(2/0) BsrFI 323 GCCGGT [GA]^CCGG[CT] BstXI 788 CCAACCTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 219 CCTGAGG CC^T[ACGT]AGG CfoI 1067 GCGC GCG^C Cfr10I 323 GCCGGT [GA]^CCGG[CT] DrdI 370 GACAAAGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 1021 CTCTTC CTCTTC(1/4) Eco81I 219 CCTGAGG CC^T[ACGT]AGG FauI 78 GCGGG CCCGC(4/6) FbaI 230 TGATCA T^GATCA HhaI 1067 GCGC GCG^C Hin1I 997 GACGTC G[GA]^CG[CT]C HinP1I 1067 GCGC G^CGC Ksp632I 1021 CTCTTC CTCTTC(1/4) MfeI 59 CAATTG C^AATTG MluNI 188 TGGCCA TGG^CCA MroI 380 TCCGGA T^CCGGA MscI 188 TGGCCA TGG^CCA MspA1I 954 CCGCTG C[AC]G^C[GT]G MunI 59 CAATTG C^AATTG RsaI 336 GTAC GT^AC TaqI 368 TCGA T^CGA TfiI 138 GATTC G^A[AT]TC TthHB8I 368 TCGA T^CGA XcmI 251 CCAAGGCCAACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmnI 466 GAAACCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 11 enzymes) # ################################### BsaWI 380 TCCGGA [AT]^CCGG[AT] 524 ACCGGA BsrI 620 ACTGG ACTGG(1/-1) 213 CCAGT Cac8I 756 GCACGC GC[ACGT]^[ACGT]GC 956 GCTGGC DraII 705 GGGGCCC [GA]G^G[ACGT]CC[CT] 706 GGGCCCC EaeI 188 TGGCCA [CT]^GGCC[GA] 797 TGGCCG EcoO109I 705 GGGGCCC [GA]G^G[ACGT]CC[CT] 706 GGGCCCC HphI 785 TCACC GGTGA(8/7) 851 TCACC MaeII 334 ACGT A^CGT 998 ACGT MaeIII 283 GTGAC ^GT[ACGT]AC 535 GTCAC Tsp45I 283 GTGAC ^GT[GC]AC 535 GTCAC Tsp509I 24 AATT ^AATT 60 AATT ######################################### # 3 OR MORE CUT ENZYMES ( 57 enzymes) # ######################################### AciI AluI AlwI AlwNI AspHI AvaII BanII BbvI BcnI BmyI BsaJI BseRI BsiHKAI BsiYI BslI BsmFI Bsp1286I BssKI BstF5I BstNI BstYI Cfr13I DdeI DpnII EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HinfI HpaII ItaI MboI MboII MflI MnlI MslI MspI MvaI MwoI NciI NdeII NlaIII NlaIV PleI PstI SacI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TseI TspRI XhoII