(Circular) (Six-base) MAPSORT of: pFN21AE4074.seq Check: 2646 from: 1 to: 5905 pFN21AE4074 5905 bp With 182 enzymes: SgfI * March 28, 2012 13:14 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3006 3715 278 Size: 53 83 186 936 1470 709 2468 Fragments arranged by size: 2468 1470 936 709 186 83 53 AccI GT'mk_AC Cuts at: 1937 3248 1937 Size: 1311 4594 Acc65I G'GTAC_C Cuts at: 3226 3226 Size: 5905 AclI AA'CG_TT Cuts at: 1563 4032 4405 1563 Size: 2469 373 3063 Fragments arranged by size: 3063 2469 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2072 3968 5352 5620 1632 Size: 440 1896 1384 268 1917 Fragments arranged by size: 1917 1896 1384 440 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5708 AflIII A'CryG_T Cuts at: 1203 2337 4804 5781 1203 Size: 1134 2467 977 1327 Fragments arranged by size: 2467 1327 1134 977 AgeI A'CCGG_T Cuts at: 4736 4736 Size: 5905 AhdI GACnn_n'nnGTC Cuts at: 4634 4634 Size: 5905 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5905 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5905 AlwNI CAG_nnn'CTG Cuts at: 2224 3103 3172 3648 5220 2224 Size: 879 69 476 1572 2909 Fragments arranged by size: 2909 1572 879 476 69 ApaI G_GGCC'C Cuts at: 2715 2715 Size: 5905 ApaLI G'TGCA_C Cuts at: 3962 5118 3962 Size: 1156 4749 ApoI r'AATT_y Cuts at: 1493 3214 3440 3592 5701 1493 Size: 1721 226 152 2109 1697 Fragments arranged by size: 2109 1721 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4459 160 Size: 872 3427 1606 Fragments arranged by size: 3427 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5905 AvaI C'yCGr_G Cuts at: 1870 1943 3230 1870 Size: 73 1287 4545 Fragments arranged by size: 4545 1287 73 BamHI G'GATC_C Cuts at: 2643 3235 3602 2643 Size: 592 367 4946 Fragments arranged by size: 4946 592 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2127 3226 4681 618 Size: 325 217 627 340 1099 1455 1842 Fragments arranged by size: 1842 1455 1099 627 340 325 217 BanII G_rGCy'C Cuts at: 1391 2061 2555 2715 2910 3192 3224 1391 Size: 670 494 160 195 282 32 4072 Fragments arranged by size: 4072 670 494 282 195 160 32 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4934 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4096 4130 1903 Size: 34 2159 34 3678 Fragments arranged by size: 3678 2159 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3797 5007 3797 Size: 1210 4695 BclI T'GATC_A Cuts at: 1548 2235 1548 Size: 687 5218 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4516 136 Size: 107 122 71 631 605 2844 1525 Fragments arranged by size: 2844 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5900 5900 Size: 5905 BlpI GC'TnA_GC Cuts at: 3322 3322 Size: 5905 Bme1580I G_kGCm'C Cuts at: 1790 2399 2715 3966 5122 1790 Size: 609 316 1251 1156 2573 Fragments arranged by size: 2573 1251 1156 609 316 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5905 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2212 2635 3636 Size: 416 53 83 399 24 795 423 1001 Cuts at: 3636 4594 442 Size: 958 1753 Fragments arranged by size: 1753 1001 958 795 423 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5905 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2191 2191 Size: 5905 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4565 1239 Size: 3326 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4030 4895 5193 4030 Size: 865 298 4742 Fragments arranged by size: 4742 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4568 882 Size: 617 3069 2219 Fragments arranged by size: 3069 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2340 3696 493 Size: 711 1136 1356 2702 Fragments arranged by size: 2702 1356 1136 711 BsaBI GATnn'nnATC Cuts at: 5899 5899 Size: 5905 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3003 3712 4094 4729 Size: 53 83 186 936 1470 709 382 635 Cuts at: 4729 275 Size: 1451 Fragments arranged by size: 1470 1451 936 709 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2385 2529 4338 4736 5010 5157 5568 2385 Size: 144 1809 398 274 147 411 2722 Fragments arranged by size: 2722 1809 411 398 274 147 144 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5680 Fragments arranged by size: 5680 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2432 3047 3050 1186 Size: 1246 615 3 4041 Fragments arranged by size: 4041 1246 615 3 BseYI C'CCAG_C Cuts at: 5108 5604 5108 Size: 496 5409 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2000 1163 Size: 573 264 5068 Fragments arranged by size: 5068 573 264 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3355 4116 5144 1540 Size: 275 186 1354 761 1028 2301 Fragments arranged by size: 2301 1354 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2555 2763 2910 3192 3966 4051 5122 2555 Size: 208 147 282 774 85 1071 3338 Fragments arranged by size: 3338 1071 774 282 208 147 85 BsmI GAATG_Cn' Cuts at: 3416 3509 3416 Size: 93 5812 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2061 2399 2555 2715 2763 2910 3192 Size: 399 271 338 156 160 48 147 282 Cuts at: 3192 3224 3966 4051 5122 1391 Size: 32 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 338 282 271 160 156 147 85 48 32 BspEI T'CCGG_A Cuts at: 2385 5568 2385 Size: 3183 2722 BspHI T'CATG_A Cuts at: 3794 5524 3794 Size: 1730 4175 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3260 844 Size: 2416 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2641 3792 1394 Size: 429 818 1151 3507 Fragments arranged by size: 3507 1151 818 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2250 4400 4574 65 Size: 1183 1002 2150 174 1396 Fragments arranged by size: 2150 1396 1183 1002 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2328 4549 4736 1700 Size: 110 518 2221 187 2869 Fragments arranged by size: 2869 2221 518 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5905 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5905 BssSI C'ACGA_G Cuts at: 3965 4977 3965 Size: 1012 4893 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5905 BstBI TT'CG_AA Cuts at: 4709 4709 Size: 5905 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5905 BstXI CCAn_nnnn'nTGG Cuts at: 2800 2800 Size: 5905 BstYI r'GATC_y Cuts at: 1972 2643 2808 3168 3235 3602 3987 4004 5456 Size: 671 165 360 67 367 385 17 1452 Cuts at: 5456 5542 5554 5900 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 671 385 367 360 346 165 86 67 17 12 Bsu36I CC'TnA_GG Cuts at: 2225 2225 Size: 5905 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5353 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2662 487 Size: 683 56 1436 3730 Fragments arranged by size: 3730 1436 683 56 BtsI GCAGTG_nn' Cuts at: 2852 2969 3421 4215 4235 2852 Size: 117 452 794 20 4522 Fragments arranged by size: 4522 794 452 117 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5905 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5870 DraI TTT'AAA Cuts at: 3209 3209 Size: 5905 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2381 4912 809 Size: 507 1065 2531 1802 Fragments arranged by size: 2531 1802 1065 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2193 2802 3352 Size: 54 1425 50 275 18 363 609 550 Cuts at: 3352 4241 5870 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 609 550 363 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3352 1537 Size: 275 1540 4090 Fragments arranged by size: 4090 1540 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3032 3835 1353 Size: 1679 803 3423 Fragments arranged by size: 3423 1679 803 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2133 4492 4866 5012 387 Size: 1064 138 544 2359 374 146 1280 Fragments arranged by size: 2359 1280 1064 544 374 146 138 EcoICRI GAG'CTC Cuts at: 2553 2908 3190 2553 Size: 355 282 5268 Fragments arranged by size: 5268 355 282 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2072 3968 4565 5352 5620 1239 Size: 393 440 1896 597 787 268 1524 Fragments arranged by size: 1896 1524 787 597 440 393 268 EcoO109I rG'GnC_Cy Cuts at: 2711 2712 2711 Size: 1 5904 EcoRI G'AATT_C Cuts at: 3214 3592 3214 Size: 378 5527 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2164 2164 Size: 5905 FspI TGC'GCA Cuts at: 4411 4411 Size: 5905 HaeII r_GCGC'y Cuts at: 1678 5052 1678 Size: 3374 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4546 4578 4620 4652 1373 Size: 32 135 32 2974 32 42 32 2626 Fragments arranged by size: 2974 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3249 3495 669 Size: 988 281 1311 246 3079 Fragments arranged by size: 3079 1311 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5905 HpaI GTT'AAC Cuts at: 3495 3495 Size: 5905 KpnI G_GTAC'C Cuts at: 3230 3230 Size: 5905 MfeI C'AATT_G Cuts at: 2064 3504 2064 Size: 1440 4465 MluI A'CGCG_T Cuts at: 5781 5781 Size: 5905 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5019 5203 1305 Size: 3714 184 2007 Fragments arranged by size: 3714 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2195 10 Size: 54 1425 343 363 3720 Fragments arranged by size: 3720 1425 363 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2270 2546 3080 3863 4222 4381 518 Size: 648 1104 276 534 783 359 159 2042 Fragments arranged by size: 2042 1104 783 648 534 359 276 159 MspA1I CmG'CkG Cuts at: 1606 2961 3321 3998 5146 5391 1606 Size: 1355 360 677 1148 245 2120 Fragments arranged by size: 2120 1355 1148 677 360 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5353 NdeI CA'TA_TG Cuts at: 387 387 Size: 5905 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5905 NotI GC'GGCC_GC Cuts at: 3352 3352 Size: 5905 NspI r_CATG'y Cuts at: 1132 1229 3263 4808 1132 Size: 97 2034 1545 2229 Fragments arranged by size: 2229 2034 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5905 PciI A'CATG_T Cuts at: 4804 4804 Size: 5905 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5905 PfoI T'CCnGG_A Cuts at: 2040 2448 2811 3090 2040 Size: 408 363 279 4855 Fragments arranged by size: 4855 408 363 279 PmeI GTTT'AAAC Cuts at: 3209 3209 Size: 5905 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3974 4006 5487 5519 3974 Size: 32 1481 32 4360 Fragments arranged by size: 4360 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5905 PsiI TTA'TAA Cuts at: 3475 3475 Size: 5905 PspOMI G'GGCC_C Cuts at: 2711 2711 Size: 5905 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2204 2236 2204 Size: 32 5873 PstI C_TGCA'G Cuts at: 830 2370 3141 3177 3257 830 Size: 1540 771 36 80 3478 Fragments arranged by size: 3478 1540 771 80 36 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5905 SacI G_AGCT'C Cuts at: 2555 2910 3192 2555 Size: 355 282 5268 Fragments arranged by size: 5268 355 282 SalI G'TCGA_C Cuts at: 1936 3247 1936 Size: 1311 4594 SbfI CC_TGCA'GG Cuts at: 3257 3257 Size: 5905 ScaI AGT'ACT Cuts at: 4153 5865 4153 Size: 1712 4193 SfcI C'TryA_G Cuts at: 826 1045 2366 3137 3173 3253 4388 5069 5260 Size: 219 1321 771 36 80 1135 681 191 Cuts at: 5260 826 Size: 1471 Fragments arranged by size: 1471 1321 1135 771 681 219 191 80 36 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5905 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5905 SmaI CCC'GGG Cuts at: 1872 3232 1872 Size: 1360 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4009 4910 5172 820 Size: 197 926 2066 901 262 1553 Fragments arranged by size: 2066 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3696 493 Size: 3203 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5905 SphI G_CATG'C Cuts at: 1132 3263 1132 Size: 2131 3774 SspI AAT'ATT Cuts at: 5 52 3829 5 Size: 47 3777 2081 Fragments arranged by size: 3777 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5905 StyI C'CwwG_G Cuts at: 513 1065 2163 2256 3183 3344 513 Size: 552 1098 93 927 161 3074 Fragments arranged by size: 3074 1098 927 552 161 93 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2258 3932 3949 4102 4287 4732 325 Size: 1255 678 1674 17 153 185 445 1498 Fragments arranged by size: 1674 1498 1255 678 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4151 5863 96 Size: 274 80 33 51 1443 2174 1712 138 Fragments arranged by size: 2174 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2518 2550 5865 5897 2518 Size: 32 3315 32 2526 Fragments arranged by size: 3315 2526 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5905 XbaI T'CTAG_A Cuts at: 3241 3241 Size: 5905 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2263 2263 Size: 5905 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5905 XmaI C'CCGG_G Cuts at: 1870 3230 1870 Size: 1360 4545 XmnI GAAnn'nnTTC Cuts at: 2475 4034 2475 Size: 1559 4346 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3004 3713 276 Size: 53 83 186 936 1470 709 2468 Fragments arranged by size: 2468 1470 936 709 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PsrI PstI PvuI SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspXI PvuII RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI