(Linear) (Six-base) MAPSORT of: KIEE4074.seq Check: 4797 from: 1 to: 1200 KIEE4074.orf With 182 enzymes: SgfI * March 22, 2012 17:19 .. AatII G_ACGT'C Cuts at: 0 1001 1200 Size: 1001 199 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 67 1200 Size: 67 1133 AflIII A'CryG_T Cuts at: 0 332 1200 Size: 332 868 AlwNI CAG_nnn'CTG Cuts at: 0 219 1098 1167 1200 Size: 219 879 69 33 Fragments arranged by size: 879 219 69 33 ApaI G_GGCC'C Cuts at: 0 710 1200 Size: 710 490 BamHI G'GATC_C Cuts at: 0 638 1200 Size: 638 562 BanI G'GyrC_C Cuts at: 0 122 1200 Size: 122 1078 BanII G_rGCy'C Cuts at: 0 56 550 710 905 1187 1200 Size: 56 494 160 195 282 13 Fragments arranged by size: 494 282 195 160 56 13 BclI T'GATC_A Cuts at: 0 230 1200 Size: 230 970 Bme1580I G_kGCm'C Cuts at: 0 394 710 1200 Size: 394 316 490 Fragments arranged by size: 490 394 316 BmrI ACTGGGnnnn_n' Cuts at: 0 207 630 1200 Size: 207 423 570 Fragments arranged by size: 570 423 207 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 186 1200 Size: 186 1014 BsaAI yAC'GTr Cuts at: 0 335 1200 Size: 335 865 BsaHI Gr'CG_yC Cuts at: 0 998 1200 Size: 998 202 BsaWI w'CCGG_w Cuts at: 0 380 524 1200 Size: 380 144 676 Fragments arranged by size: 676 380 144 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 427 1042 1045 1200 Size: 427 615 3 155 Fragments arranged by size: 615 427 155 3 BsiHKAI G_wGCw'C Cuts at: 0 550 758 905 1187 1200 Size: 550 208 147 282 13 Fragments arranged by size: 550 282 208 147 13 Bsp1286I G_dGCh'C Cuts at: 0 56 394 550 710 758 905 1187 1200 Size: 56 338 156 160 48 147 282 13 Fragments arranged by size: 338 282 160 156 147 56 48 13 BspEI T'CCGG_A Cuts at: 0 380 1200 Size: 380 820 BsrBI CCG'CTC Cuts at: 0 636 1200 Size: 636 564 BsrDI GCAATG_nn' Cuts at: 0 245 1200 Size: 245 955 BsrFI r'CCGG_y Cuts at: 0 323 1200 Size: 323 877 BstXI CCAn_nnnn'nTGG Cuts at: 0 795 1200 Size: 795 405 BstYI r'GATC_y Cuts at: 0 638 803 1163 1200 Size: 638 165 360 37 Fragments arranged by size: 638 360 165 37 Bsu36I CC'TnA_GG Cuts at: 0 220 1200 Size: 220 980 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 657 1200 Size: 657 543 BtsI GCAGTG_nn' Cuts at: 0 847 964 1200 Size: 847 117 236 Fragments arranged by size: 847 236 117 DrdI GACnn_nn'nnGTC Cuts at: 0 376 1200 Size: 376 824 EaeI y'GGCC_r Cuts at: 0 188 797 1200 Size: 188 609 403 Fragments arranged by size: 609 403 188 EarI CTCTTCn'nnn_ Cuts at: 0 1027 1200 Size: 1027 173 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 128 1200 Size: 128 1072 EcoICRI GAG'CTC Cuts at: 0 548 903 1185 1200 Size: 548 355 282 15 Fragments arranged by size: 548 355 282 15 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 67 1200 Size: 67 1133 EcoO109I rG'GnC_Cy Cuts at: 0 706 707 1200 Size: 706 1 493 Fragments arranged by size: 706 493 1 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 159 1200 Size: 159 1041 MfeI C'AATT_G Cuts at: 0 59 1200 Size: 59 1141 MscI TGG'CCA Cuts at: 0 190 1200 Size: 190 1010 MslI CAynn'nnrTG Cuts at: 0 265 541 1075 1200 Size: 265 276 534 125 Fragments arranged by size: 534 276 265 125 MspA1I CmG'CkG Cuts at: 0 956 1200 Size: 956 244 PfoI T'CCnGG_A Cuts at: 0 35 443 806 1085 1200 Size: 35 408 363 279 115 Fragments arranged by size: 408 363 279 115 35 PspOMI G'GGCC_C Cuts at: 0 706 1200 Size: 706 494 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 199 231 1200 Size: 199 32 969 Fragments arranged by size: 969 199 32 PstI C_TGCA'G Cuts at: 0 365 1136 1172 1200 Size: 365 771 36 28 Fragments arranged by size: 771 365 36 28 SacI G_AGCT'C Cuts at: 0 550 905 1187 1200 Size: 550 355 282 13 Fragments arranged by size: 550 355 282 13 SfcI C'TryA_G Cuts at: 0 361 1132 1168 1200 Size: 361 771 36 32 Fragments arranged by size: 771 361 36 32 StyI C'CwwG_G Cuts at: 0 158 251 1178 1200 Size: 158 93 927 22 Fragments arranged by size: 927 158 93 22 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 253 1200 Size: 253 947 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 513 545 1200 Size: 513 32 655 Fragments arranged by size: 655 513 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 258 1200 Size: 258 942 XmnI GAAnn'nnTTC Cuts at: 0 470 1200 Size: 470 730 ZraI GAC'GTC Cuts at: 0 999 1200 Size: 999 201 Enzymes that do cut: AatII AcuI AflIII AlwNI ApaI BamHI BanI BanII BclI Bme1580I BmrI BplI BsaAI BsaHI BsaWI BseRI BsiHKAI Bsp1286I BspEI BsrBI BsrDI BsrFI BstXI BstYI Bsu36I BtgZI BtsI DrdI EaeI EarI EciI EcoICRI Eco57MI EcoO109I FalI MfeI MscI MslI MspA1I PfoI PspOMI PsrI PstI SacI SfcI StyI TaqII TstI XcmI XmnI ZraI Enzymes that do not cut: AarI AccI Acc65I AclI AfeI AflII AgeI AhdI AleI AlfI AloI ApaLI ApoI AscI AseI AsiSI AvaI AvrII BaeI BbeI BbsI BbvCI BcgI BciVI BfrBI BglI BglII BlpI BmgBI BmtI BpmI Bpu10I BpuEI BsaI BsaBI BsaXI BseYI BsgI BsiEI BsiWI BsmI BsmBI BspHI BspMI BsrGI BssHII BssSI BstAPI BstBI BstEII BstZ17I BtgI ClaI CspCI DraI DraIII EagI EcoNI EcoRI EcoRV FseI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MluI MmeI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PvuI PvuII RsrII SacII SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI TatI Tth111I XbaI XhoI XmaI