Restriction map for
pFN21AE4166
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# NO CUT ENZYMES ( 88 enzymes) #
##################################
Acc65I AclI AcsI AflII AflIII
AgeI Alw44I Aor51HI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspI BcgI BclI BfrI BglII
BlpI Bpu1102I BsiWI BsmI Bsp1407I
BspDI BspLU11I BsrDI BsrGI BssHII
Bst1107I BstZ17I CelII ClaI CpoI
DraI DrdI Eco47III EcoRI EcoRV
EcoT22I FbaI HincII HindII HindIII
HpaI KpnI MfeI MluI MseI
MunI NdeI NheI NotI NruI
NsiI NspI PacI PaeR7I PflFI
PinAI PleI PmeI PshAI PshBI
Psp1406I PvuI PvuII RsrII SalI
SexAI SgrAI SmaI SmlI SnaBI
SpeI SphI SspBI SspI SwaI
Tru9I Tsp509I Tth111I XbaI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AccI 1519 GTCTAC GT^[AC][GT]AC
AccIII 1118 TCCGGA T^CCGGA
AfaI 1395 GTAC GT^AC
AviII 14 TGCGCA TGC^GCA
AvrII 1137 CCTAGG C^CTAGG
BalI 1433 TGGCCA TGG^CCA
BamHI 290 GGATCC G^GATCC
BbeI 964 GGCGCC GGCGC^C
BbrPI 874 CACGTG CAC^GTG
BbvCI 1815 CCTCAGC CCTCAGC(-5/-2)
BciVI 1078 GGATAC GTATCC(6/5)
BlnI 1137 CCTAGG C^CTAGG
BsaAI 874 CACGTG [CT]AC^GT[GA]
BsaBI 117 GATCACGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 1118 TCCGGA [AT]^CCGG[AT]
BseAI 1118 TCCGGA T^CCGGA
BsmBI 249 CGTCTC CGTCTC(1/5)
BspEI 1118 TCCGGA T^CCGGA
BspHI 401 TCATGA T^CATGA
BsrBI 1646 CCGCTC CCGCTC(-3/-3)
BstAPI 1504 GCACATTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 278 TTCGAA TT^CGAA
BstEII 1101 GGTGACC G^GT[ACGT]ACC
BstPI 1101 GGTGACC G^GT[ACGT]ACC
BstXI 1436 CCACATCGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
EcoO65I 1101 GGTGACC G^GT[ACGT]ACC
FseI 1056 GGCCGGCC GGCCGG^CC
FspI 14 TGCGCA TGC^GCA
HinfI 276 GATTC G^A[ACGT]TC
KasI 964 GGCGCC G^GCGCC
MamI 117 GATCACGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 1433 TGGCCA TGG^CCA
MroI 1118 TCCGGA T^CCGGA
MscI 1433 TGGCCA TGG^CCA
MslI 339 CATCCTAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 964 GGCGCC GG^CGCC
NspV 278 TTCGAA TT^CGAA
PmaCI 874 CACGTG CAC^GTG
PmlI 874 CACGTG CAC^GTG
RcaI 401 TCATGA T^CATGA
RsaI 1395 GTAC GT^AC
SbfI 1296 CCTGCAGG CCTGCA^GG
ScaI 1394 AGTACT AGT^ACT
SfoI 964 GGCGCC GGC^GCC
SfuI 278 TTCGAA TT^CGAA
Sse8387I 1296 CCTGCAGG CCTGCA^GG
StuI 1301 AGGCCT AGG^CCT
TfiI 276 GATTC G^A[AT]TC
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# TWO CUT ENZYMES ( 27 enzymes) #
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AhdI 361 GACCTGCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1489 GACCCAAAGTC
AocI 453 CCTGAGG CC^T[ACGT]AGG
526 CCTGAGG
AspEI 361 GACCTGCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1489 GACCCAAAGTC
BbsI 48 GAAGAC GAAGAC(2/6)
687 GAAGAC
BfaI 343 CTAG C^TAG
1138 CTAG
BpmI 676 CTGGAG CTGGAG(16/14)
838 CTGGAG
BpuAI 48 GAAGAC GAAGAC(2/6)
687 GAAGAC
BsaI 424 GAGACC GGTCTC(1/5)
793 GAGACC
BstYI 212 AGATCC [GA]^GATC[CT]
290 GGATCC
Bsu36I 453 CCTGAGG CC^T[ACGT]AGG
526 CCTGAGG
DraIII 160 CACATCGTG CAC[ACGT][ACGT][ACGT]^GTG
1505 CACATTGTG
Eam1105I 361 GACCTGCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1489 GACCCAAAGTC
Eco81I 453 CCTGAGG CC^T[ACGT]AGG
526 CCTGAGG
KspI 128 CCGCGG CCGC^GG
1314 CCGCGG
MaeI 343 CTAG C^TAG
1138 CTAG
MflI 212 AGATCC [GA]^GATC[CT]
290 GGATCC
NaeI 785 GCCGGC GCC^GGC
1057 GCCGGC
NcoI 380 CCATGG C^CATGG
1715 CCATGG
NgoMIV 785 GCCGGC G^CCGGC
1057 GCCGGC
PflMI 159 CCACATCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1530 CCATTACATGG
PstI 888 CTGCAG CTGCA^G
1297 CTGCAG
SacII 128 CCGCGG CCGC^GG
1314 CCGCGG
SfiI 663 GGCCCCGCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
1797 GGCCGCCGTGGCC
TaqI 279 TCGA T^CGA
1364 TCGA
TthHB8I 279 TCGA T^CGA
1364 TCGA
Van91I 159 CCACATCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1530 CCATTACATGG
XhoII 212 AGATCC [GA]^GATC[CT]
290 GGATCC
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# 3 OR MORE CUT ENZYMES ( 95 enzymes) #
#########################################
AatII AccII AciI AcyI AluI
AlwI AlwNI ApaI AspHI AvaI
AvaII BanI BanII BbvI BcnI
BglI BmyI BsaHI BsaJI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrFI BsrI BssKI BssSI BstF5I
BstNI BstUI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EagI EarI EclXI Eco52I
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HpaII
HphI ItaI Ksp632I MaeII MaeIII
MboI MboII MnlI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV PpuMI SacI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
StyI TseI Tsp45I TspRI XcmI