(Circular) (Six-base) MAPSORT of: pFN21AE4166.seq Check: 9077 from: 1 to: 6559 pFN21AE4166 6559 bp With 182 enzymes: SgfI * July 19, 2019 13:42 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2060 2142 2256 3123 Size: 53 83 186 936 524 82 114 867 Cuts at: 3123 4369 278 Size: 1246 2468 Fragments arranged by size: 2468 1246 936 867 524 186 114 83 82 53 AccI GT'mk_AC Cuts at: 1937 3525 3902 1937 Size: 1588 377 4594 Fragments arranged by size: 4594 1588 377 Acc65I G'GTAC_C Cuts at: 3880 3880 Size: 6559 AclI AA'CG_TT Cuts at: 1563 4686 5059 1563 Size: 3123 373 3063 Fragments arranged by size: 3123 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2566 4622 6006 6274 1632 Size: 934 2056 1384 268 1917 Fragments arranged by size: 2056 1917 1384 934 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6362 AflIII A'CryG_T Cuts at: 1203 5458 6435 1203 Size: 4255 977 1327 Fragments arranged by size: 4255 1327 977 AgeI A'CCGG_T Cuts at: 5390 5390 Size: 6559 AhdI GACnn_n'nnGTC Cuts at: 2371 3499 5288 2371 Size: 1128 1789 3642 Fragments arranged by size: 3642 1789 1128 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6559 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6559 AlwNI CAG_nnn'CTG Cuts at: 2569 2602 2680 4302 5874 2569 Size: 33 78 1622 1572 3254 Fragments arranged by size: 3254 1622 1572 78 33 ApaI G_GGCC'C Cuts at: 2331 3032 3277 2331 Size: 701 245 5613 Fragments arranged by size: 5613 701 245 ApaLI G'TGCA_C Cuts at: 4616 5772 4616 Size: 1156 5403 ApoI r'AATT_y Cuts at: 1493 3868 4094 4246 6355 1493 Size: 2375 226 152 2109 1697 Fragments arranged by size: 2375 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5113 160 Size: 872 4081 1606 Fragments arranged by size: 4081 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6559 AvaI C'yCGr_G Cuts at: 1870 1943 2093 2420 3032 3884 1870 Size: 73 150 327 612 852 4545 Fragments arranged by size: 4545 852 612 327 150 73 AvrII C'CTAG_G Cuts at: 3142 3142 Size: 6559 BamHI G'GATC_C Cuts at: 2295 3889 4256 2295 Size: 1594 367 4598 Fragments arranged by size: 4598 1594 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2155 2335 2658 2969 3330 Size: 325 217 627 368 180 323 311 361 Cuts at: 3330 3880 5335 618 Size: 550 1455 1842 Fragments arranged by size: 1842 1455 627 550 368 361 325 323 311 217 180 BanII G_rGCy'C Cuts at: 1391 2331 2384 2631 2907 3032 3039 3277 3690 Size: 940 53 247 276 125 7 238 413 Cuts at: 3690 3878 1391 Size: 188 4072 Fragments arranged by size: 4072 940 413 276 247 238 188 125 53 7 BbeI G_GCGC'C Cuts at: 2973 2973 Size: 6559 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2060 2699 928 Size: 971 161 639 4788 Fragments arranged by size: 4788 971 639 161 BbvCI CC'TCA_GC Cuts at: 3821 3821 Size: 6559 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4750 4784 1903 Size: 34 2813 34 3678 Fragments arranged by size: 3678 2813 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3077 4451 5661 3077 Size: 1374 1210 3975 Fragments arranged by size: 3975 1374 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6559 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2495 2675 3722 Size: 107 122 71 631 605 823 180 1047 Cuts at: 3722 3809 5170 136 Size: 87 1361 1525 Fragments arranged by size: 1525 1361 1047 823 631 605 180 122 107 87 71 BglII A'GATC_T Cuts at: 6554 6554 Size: 6559 BlpI GC'TnA_GC Cuts at: 3976 3976 Size: 6559 Bme1580I G_kGCm'C Cuts at: 1790 2160 2331 2340 2661 2886 3032 3277 3333 Size: 370 171 9 321 225 146 245 56 Cuts at: 3333 3425 4620 5776 1790 Size: 92 1195 1156 2573 Fragments arranged by size: 2573 1195 1156 370 321 245 225 171 146 92 56 9 BmgBI CAC'GTC Cuts at: 1318 2017 2689 1318 Size: 699 672 5188 Fragments arranged by size: 5188 699 672 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2042 4290 5248 Size: 416 53 83 399 24 625 2248 958 Cuts at: 5248 442 Size: 1753 Fragments arranged by size: 2248 1753 958 625 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6559 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2702 2864 5219 1239 Size: 1463 162 2355 2579 Fragments arranged by size: 2579 2355 1463 162 Bpu10I CC'TnA_GC Cuts at: 3821 3821 Size: 6559 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4684 5549 5847 4684 Size: 865 298 5396 Fragments arranged by size: 5396 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2423 2792 5222 882 Size: 617 924 369 2430 2219 Fragments arranged by size: 2430 2219 924 617 369 BsaAI yAC'GTr Cuts at: 493 1204 2881 4350 493 Size: 711 1677 1469 2702 Fragments arranged by size: 2702 1677 1469 711 BsaBI GATnn'nnATC Cuts at: 2126 6553 2126 Size: 4427 2132 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2057 2139 2253 2970 Size: 53 83 186 936 524 82 114 717 Cuts at: 2970 3120 3453 4366 4748 5383 275 Size: 150 333 913 382 635 1451 Fragments arranged by size: 1451 936 913 717 635 524 382 333 186 150 114 83 82 53 BsaWI w'CCGG_w Cuts at: 3123 4992 5390 5664 5811 6222 3123 Size: 1869 398 274 147 411 3460 Fragments arranged by size: 3460 1869 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2849 2879 1178 Size: 30 165 30 1446 30 4858 Fragments arranged by size: 4858 1446 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2099 2111 2132 2216 2228 2276 3562 1186 Size: 913 12 21 84 12 48 1286 4183 Fragments arranged by size: 4183 1286 913 84 48 21 12 12 BseYI C'CCAG_C Cuts at: 3013 3679 3689 3708 3787 5762 6258 3013 Size: 666 10 19 79 1975 496 3314 Fragments arranged by size: 3314 1975 666 496 79 19 10 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2725 2951 3062 3398 1163 Size: 573 989 226 111 336 4324 Fragments arranged by size: 4324 989 573 336 226 111 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2375 2791 3067 3319 3624 4009 Size: 275 186 374 416 276 252 305 385 Cuts at: 4009 4770 5798 1540 Size: 761 1028 2301 Fragments arranged by size: 2301 1028 761 416 385 374 305 276 275 252 186 BsiHKAI G_wGCw'C Cuts at: 2384 2400 2631 2907 3039 4620 4705 5776 2384 Size: 16 231 276 132 1581 85 1071 3167 Fragments arranged by size: 3167 1581 1071 276 231 132 85 16 BsmI GAATG_Cn' Cuts at: 4070 4163 4070 Size: 93 6466 BsmBI CGTCTCn'nnnn_ Cuts at: 2260 2260 Size: 6559 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2160 2331 2340 2384 2400 2631 2661 Size: 399 370 171 9 44 16 231 30 Cuts at: 2661 2886 2907 3032 3039 3277 3333 3425 3690 Size: 225 21 125 7 238 56 92 265 Cuts at: 3690 3878 4620 4705 5776 1391 Size: 188 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 370 265 238 231 225 188 171 125 92 85 56 44 30 21 16 9 7 BspEI T'CCGG_A Cuts at: 3123 6222 3123 Size: 3099 3460 BspHI T'CATG_A Cuts at: 2406 4448 6178 2406 Size: 2042 1730 2787 Fragments arranged by size: 2787 2042 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2376 2441 3370 3594 3914 844 Size: 1532 65 929 224 320 3489 Fragments arranged by size: 3489 1532 929 320 224 65 BsrBI CCG'CTC Cuts at: 1394 1823 3653 4446 1394 Size: 429 1830 793 3507 Fragments arranged by size: 3507 1830 793 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5054 5228 65 Size: 1183 3806 174 1396 Fragments arranged by size: 3806 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2736 2790 3062 3066 3619 3642 5203 Size: 110 926 54 272 4 553 23 1561 Cuts at: 5203 5390 1700 Size: 187 2869 Fragments arranged by size: 2869 1561 926 553 272 187 110 54 23 4 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6559 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6559 BssSI C'ACGA_G Cuts at: 2795 3204 3340 4619 5631 2795 Size: 409 136 1279 1012 3723 Fragments arranged by size: 3723 1279 1012 409 136 BstAPI GCAn_nnn'nTGC Cuts at: 794 3515 794 Size: 2721 3838 BstBI TT'CG_AA Cuts at: 2284 5363 2284 Size: 3079 3480 BstEII G'GTnAC_C Cuts at: 1184 3106 1184 Size: 1922 4637 BstXI CCAn_nnnn'nTGG Cuts at: 3448 3448 Size: 6559 BstYI r'GATC_y Cuts at: 1972 2217 2295 3889 4256 4641 4658 6110 6196 Size: 245 78 1594 367 385 17 1452 86 Cuts at: 6196 6208 6554 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1594 1452 385 367 346 245 86 78 17 12 Bsu36I CC'TnA_GG Cuts at: 2459 2532 2459 Size: 73 6486 BtgI C'CryG_G Cuts at: 513 1065 2133 2385 2982 3277 3319 3720 3807 Size: 552 1068 252 597 295 42 401 87 Cuts at: 3807 513 Size: 3265 Fragments arranged by size: 3265 1068 597 552 401 295 252 87 42 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2850 3429 487 Size: 683 56 1624 579 3617 Fragments arranged by size: 3617 1624 683 579 56 BtsI GCAGTG_nn' Cuts at: 3496 4075 4869 4889 3496 Size: 579 794 20 5166 Fragments arranged by size: 5166 794 579 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6559 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6524 DraI TTT'AAA Cuts at: 3863 3863 Size: 6559 DraIII CAC_nnn'GTG Cuts at: 2170 3515 2170 Size: 1345 5214 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5566 809 Size: 507 4250 1802 Fragments arranged by size: 4250 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2448 2788 3064 Size: 54 1425 50 275 18 618 340 276 Cuts at: 3064 3316 3438 3621 3723 3810 4006 4895 6524 Size: 252 122 183 102 87 196 889 1629 Cuts at: 6524 8 Size: 43 Fragments arranged by size: 1629 1425 889 618 340 276 275 252 196 183 122 102 87 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2788 3064 3316 3621 4006 1537 Size: 275 976 276 252 305 385 4090 Fragments arranged by size: 4090 976 385 305 276 275 252 EarI CTCTTCn'nnn_ Cuts at: 1353 2619 2765 2780 3206 3769 3845 4489 1353 Size: 1266 146 15 426 563 76 644 3423 Fragments arranged by size: 3423 1266 644 563 426 146 76 15 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5146 5520 5666 387 Size: 1064 138 3557 374 146 1280 Fragments arranged by size: 3557 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2382 2629 2905 3037 2382 Size: 247 276 132 5904 Fragments arranged by size: 5904 276 247 132 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2566 2702 2864 4622 5219 6006 6274 Size: 393 934 136 162 1758 597 787 268 Cuts at: 6274 1239 Size: 1524 Fragments arranged by size: 1758 1524 934 787 597 393 268 162 136 EcoNI CCTnn'n_nnAGG Cuts at: 2606 3260 3413 2606 Size: 654 153 5752 Fragments arranged by size: 5752 654 153 EcoO109I rG'GnC_Cy Cuts at: 2101 2327 2599 2677 3028 3029 3273 3841 2101 Size: 226 272 78 351 1 244 568 4819 Fragments arranged by size: 4819 568 351 272 244 226 78 1 EcoRI G'AATT_C Cuts at: 3868 4246 3868 Size: 378 6181 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3313 3313 Size: 6559 FseI GG_CCGG'CC Cuts at: 3066 3066 Size: 6559 FspI TGC'GCA Cuts at: 2021 5065 2021 Size: 3044 3515 FspAI rTGC'GCAy Cuts at: 2021 2021 Size: 6559 HaeII r_GCGC'y Cuts at: 1678 2570 2615 2824 2973 3023 5706 1678 Size: 892 45 209 149 50 2683 2531 Fragments arranged by size: 2683 2531 892 209 149 50 45 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2357 2389 2849 2881 3485 Size: 32 135 32 785 32 460 32 604 Cuts at: 3485 3517 5200 5232 5274 5306 1373 Size: 32 1683 32 42 32 2626 Fragments arranged by size: 2626 1683 785 604 460 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3903 4149 669 Size: 988 281 1965 246 3079 Fragments arranged by size: 3079 1965 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6559 HpaI GTT'AAC Cuts at: 4149 4149 Size: 6559 KasI G'GCGC_C Cuts at: 2969 2969 Size: 6559 KpnI G_GTAC'C Cuts at: 3884 3884 Size: 6559 MfeI C'AATT_G Cuts at: 4158 4158 Size: 6559 MluI A'CGCG_T Cuts at: 6435 6435 Size: 6559 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5673 5857 1305 Size: 4368 184 2007 Fragments arranged by size: 4368 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3440 10 Size: 54 1425 343 1608 3129 Fragments arranged by size: 3129 1608 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2348 4517 4876 5035 518 Size: 648 1182 2169 359 159 2042 Fragments arranged by size: 2169 2042 1182 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2135 3244 3321 3975 4652 5800 6045 1606 Size: 529 1109 77 654 677 1148 245 2120 Fragments arranged by size: 2120 1148 1109 677 654 529 245 77 NaeI GCC'GGC Cuts at: 2792 3064 2792 Size: 272 6287 NarI GG'CG_CC Cuts at: 2970 2970 Size: 6559 NcoI C'CATG_G Cuts at: 513 1065 2385 3720 513 Size: 552 1320 1335 3352 Fragments arranged by size: 3352 1335 1320 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6559 NgoMIV G'CCGG_C Cuts at: 2790 3062 2790 Size: 272 6287 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6559 NotI GC'GGCC_GC Cuts at: 4006 4006 Size: 6559 NspI r_CATG'y Cuts at: 1132 1229 3917 5462 1132 Size: 97 2688 1545 2229 Fragments arranged by size: 2688 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2331 3024 1348 Size: 983 693 4883 Fragments arranged by size: 4883 983 693 PciI A'CATG_T Cuts at: 5458 5458 Size: 6559 PflMI CCAn_nnn'nTGG Cuts at: 1108 2170 3541 1108 Size: 1062 1371 4126 Fragments arranged by size: 4126 1371 1062 PmeI GTTT'AAAC Cuts at: 3863 3863 Size: 6559 PmlI CAC'GTG Cuts at: 2881 2881 Size: 6559 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4628 4660 6141 6173 4628 Size: 32 1481 32 5014 Fragments arranged by size: 5014 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2101 2599 3841 2101 Size: 498 1242 4819 Fragments arranged by size: 4819 1242 498 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6559 PsiI TTA'TAA Cuts at: 4129 4129 Size: 6559 PspOMI G'GGCC_C Cuts at: 2327 3028 3273 2327 Size: 701 245 5613 Fragments arranged by size: 5613 701 245 PstI C_TGCA'G Cuts at: 830 2897 3306 3911 830 Size: 2067 409 605 3478 Fragments arranged by size: 3478 2067 605 409 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6559 SacI G_AGCT'C Cuts at: 2384 2631 2907 3039 2384 Size: 247 276 132 5904 Fragments arranged by size: 5904 276 247 132 SacII CC_GC'GG Cuts at: 2136 3322 2136 Size: 1186 5373 SalI G'TCGA_C Cuts at: 1936 3901 1936 Size: 1965 4594 SapI GCTCTTCn'nnn_ Cuts at: 2619 2780 3845 2619 Size: 161 1065 5333 Fragments arranged by size: 5333 1065 161 SbfI CC_TGCA'GG Cuts at: 3306 3911 3306 Size: 605 5954 ScaI AGT'ACT Cuts at: 3401 4807 6519 3401 Size: 1406 1712 3441 Fragments arranged by size: 3441 1712 1406 SfcI C'TryA_G Cuts at: 826 1045 2830 2893 3302 3835 3907 5042 5723 Size: 219 1785 63 409 533 72 1135 681 Cuts at: 5723 5914 826 Size: 191 1471 Fragments arranged by size: 1785 1471 1135 681 533 409 219 191 72 63 SfiI GGCCn_nnn'nGGCC Cuts at: 2675 3809 2675 Size: 1134 5425 SfoI GGC'GCC Cuts at: 2971 2971 Size: 6559 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6559 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6559 SmaI CCC'GGG Cuts at: 1872 3886 1872 Size: 2014 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4663 5564 5826 820 Size: 197 926 2720 901 262 1553 Fragments arranged by size: 2720 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4350 493 Size: 3857 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6559 SphI G_CATG'C Cuts at: 1132 3917 1132 Size: 2785 3774 SspI AAT'ATT Cuts at: 5 52 4483 5 Size: 47 4431 2081 Fragments arranged by size: 4431 2081 47 StuI AGG'CCT Cuts at: 1517 3308 1517 Size: 1791 4768 StyI C'CwwG_G Cuts at: 513 1065 2385 2914 3133 3142 3576 3720 3998 Size: 552 1320 529 219 9 434 144 278 Cuts at: 3998 513 Size: 3074 Fragments arranged by size: 3074 1320 552 529 434 278 219 144 9 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2177 3722 4586 4603 4756 4941 5386 Size: 1255 597 1545 864 17 153 185 445 Cuts at: 5386 325 Size: 1498 Fragments arranged by size: 1545 1498 1255 864 597 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3399 4805 6517 Size: 274 80 33 51 1443 1422 1406 1712 Cuts at: 6517 96 Size: 138 Fragments arranged by size: 1712 1443 1422 1406 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3033 3065 3726 3758 6519 6551 3033 Size: 32 661 32 2761 32 3041 Fragments arranged by size: 3041 2761 661 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6559 XbaI T'CTAG_A Cuts at: 3895 3895 Size: 6559 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2167 2347 3021 2167 Size: 180 674 5705 Fragments arranged by size: 5705 674 180 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6559 XmaI C'CCGG_G Cuts at: 1870 3884 1870 Size: 2014 4545 XmnI GAAnn'nnTTC Cuts at: 4688 4688 Size: 6559 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2058 2140 2254 3121 Size: 53 83 186 936 524 82 114 867 Cuts at: 3121 4367 276 Size: 1246 2468 Fragments arranged by size: 2468 1246 936 867 524 186 114 83 82 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacI SacII SalI SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI BaeI BfrBI BplI BsiWI BstZ17I EcoRV NruI NsiI PacI PfoI PspXI PsrI PvuII RsrII SanDI SexAI SrfI SwaI