Restriction map for
pFN21AE4166
(Linear) (Six-base) MAPSORT of: KIEE4166.seq Check: 3534 from: 1 to: 1854
KIEE4166.orf
With 182 enzymes: SgfI *
July 16, 2019 14:21 ..
AatII G_ACGT'C
Cuts at: 0 55 137 251 1118 1854
Size: 55 82 114 867 736
Fragments arranged by size:
867 736 114 82 55
AccI GT'mk_AC
Cuts at: 0 1520 1854
Size: 1520 334
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 561 1854
Size: 561 1293
AhdI GACnn_n'nnGTC
Cuts at: 0 366 1494 1854
Size: 366 1128 360
Fragments arranged by size:
1128 366 360
AlwNI CAG_nnn'CTG
Cuts at: 0 564 597 675 1854
Size: 564 33 78 1179
Fragments arranged by size:
1179 564 78 33
ApaI G_GGCC'C
Cuts at: 0 326 1027 1272 1854
Size: 326 701 245 582
Fragments arranged by size:
701 582 326 245
AvaI C'yCGr_G
Cuts at: 0 88 415 1027 1854
Size: 88 327 612 827
Fragments arranged by size:
827 612 327 88
AvrII C'CTAG_G
Cuts at: 0 1137 1854
Size: 1137 717
BamHI G'GATC_C
Cuts at: 0 290 1854
Size: 290 1564
BanI G'GyrC_C
Cuts at: 0 150 330 653 964 1325 1854
Size: 150 180 323 311 361 529
Fragments arranged by size:
529 361 323 311 180 150
BanII G_rGCy'C
Cuts at: 0 326 379 626 902 1027 1034 1272 1685
Size: 326 53 247 276 125 7 238 413
Cuts at: 1685 1854
Size: 169
Fragments arranged by size:
413 326 276 247 238 169 125 53
7
BbeI G_GCGC'C
Cuts at: 0 968 1854
Size: 968 886
BbsI GAAGACnn'nnnn_
Cuts at: 0 55 694 1854
Size: 55 639 1160
Fragments arranged by size:
1160 639 55
BbvCI CC'TCA_GC
Cuts at: 0 1816 1854
Size: 1816 38
BciVI GTATCCnnnnn_n'
Cuts at: 0 1072 1854
Size: 1072 782
BglI GCCn_nnn'nGGC
Cuts at: 0 490 670 1717 1804 1854
Size: 490 180 1047 87 50
Fragments arranged by size:
1047 490 180 87 50
Bme1580I G_kGCm'C
Cuts at: 0 155 326 335 656 881 1027 1272 1328
Size: 155 171 9 321 225 146 245 56
Cuts at: 1328 1420 1854
Size: 92 434
Fragments arranged by size:
434 321 245 225 171 155 146 92
56 9
BmgBI CAC'GTC
Cuts at: 0 12 684 1854
Size: 12 672 1170
Fragments arranged by size:
1170 672 12
BmrI ACTGGGnnnn_n'
Cuts at: 0 37 1854
Size: 37 1817
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 697 859 1854
Size: 697 162 995
Fragments arranged by size:
995 697 162
Bpu10I CC'TnA_GC
Cuts at: 0 1816 1854
Size: 1816 38
BsaI GGTCTCn'nnnn_
Cuts at: 0 418 787 1854
Size: 418 369 1067
Fragments arranged by size:
1067 418 369
BsaAI yAC'GTr
Cuts at: 0 876 1854
Size: 876 978
BsaBI GATnn'nnATC
Cuts at: 0 121 1854
Size: 121 1733
BsaHI Gr'CG_yC
Cuts at: 0 52 134 248 965 1115 1448 1854
Size: 52 82 114 717 150 333 406
Fragments arranged by size:
717 406 333 150 114 82 52
BsaWI w'CCGG_w
Cuts at: 0 1118 1854
Size: 1118 736
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 844 874 1854
Size: 844 30 980
Fragments arranged by size:
980 844 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 94 106 127 211 223 271 1557 1854
Size: 94 12 21 84 12 48 1286 297
Fragments arranged by size:
1286 297 94 84 48 21 12 12
BseYI C'CCAG_C
Cuts at: 0 1008 1674 1684 1703 1782 1854
Size: 1008 666 10 19 79 72
Fragments arranged by size:
1008 666 79 72 19 10
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 720 946 1057 1393 1854
Size: 720 226 111 336 461
Fragments arranged by size:
720 461 336 226 111
BsiEI CG_ry'CG
Cuts at: 0 370 786 1062 1314 1619 1854
Size: 370 416 276 252 305 235
Fragments arranged by size:
416 370 305 276 252 235
BsiHKAI G_wGCw'C
Cuts at: 0 379 395 626 902 1034 1854
Size: 379 16 231 276 132 820
Fragments arranged by size:
820 379 276 231 132 16
BsmBI CGTCTCn'nnnn_
Cuts at: 0 255 1854
Size: 255 1599
Bsp1286I G_dGCh'C
Cuts at: 0 155 326 335 379 395 626 656 881
Size: 155 171 9 44 16 231 30 225
Cuts at: 881 902 1027 1034 1272 1328 1420 1685 1854
Size: 21 125 7 238 56 92 265 169
Fragments arranged by size:
265 238 231 225 171 169 155 125
92 56 44 30 21 16 9 7
BspEI T'CCGG_A
Cuts at: 0 1118 1854
Size: 1118 736
BspHI T'CATG_A
Cuts at: 0 401 1854
Size: 401 1453
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 371 436 1365 1589 1854
Size: 371 65 929 224 265
Fragments arranged by size:
929 371 265 224 65
BsrBI CCG'CTC
Cuts at: 0 1648 1854
Size: 1648 206
BsrFI r'CCGG_y
Cuts at: 0 731 785 1057 1061 1614 1637 1854
Size: 731 54 272 4 553 23 217
Fragments arranged by size:
731 553 272 217 54 23 4
BssSI C'ACGA_G
Cuts at: 0 790 1199 1335 1854
Size: 790 409 136 519
Fragments arranged by size:
790 519 409 136
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1510 1854
Size: 1510 344
BstBI TT'CG_AA
Cuts at: 0 279 1854
Size: 279 1575
BstEII G'GTnAC_C
Cuts at: 0 1101 1854
Size: 1101 753
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1443 1854
Size: 1443 411
BstYI r'GATC_y
Cuts at: 0 212 290 1854
Size: 212 78 1564
Fragments arranged by size:
1564 212 78
Bsu36I CC'TnA_GG
Cuts at: 0 454 527 1854
Size: 454 73 1327
Fragments arranged by size:
1327 454 73
BtgI C'CryG_G
Cuts at: 0 128 380 977 1272 1314 1715 1802 1854
Size: 128 252 597 295 42 401 87 52
Fragments arranged by size:
597 401 295 252 128 87 52 42
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 845 1424 1854
Size: 845 579 430
Fragments arranged by size:
845 579 430
BtsI GCAGTG_nn'
Cuts at: 0 1491 1854
Size: 1491 363
DraIII CAC_nnn'GTG
Cuts at: 0 165 1510 1854
Size: 165 1345 344
Fragments arranged by size:
1345 344 165
EaeI y'GGCC_r
Cuts at: 0 443 783 1059 1311 1433 1616 1718 1805
Size: 443 340 276 252 122 183 102 87
Cuts at: 1805 1854
Size: 49
Fragments arranged by size:
443 340 276 252 183 122 102 87
49
EagI C'GGCC_G
Cuts at: 0 783 1059 1311 1616 1854
Size: 783 276 252 305 238
Fragments arranged by size:
783 305 276 252 238
EarI CTCTTCn'nnn_
Cuts at: 0 614 760 775 1201 1764 1840 1854
Size: 614 146 15 426 563 76 14
Fragments arranged by size:
614 563 426 146 76 15 14
EcoICRI GAG'CTC
Cuts at: 0 377 624 900 1032 1854
Size: 377 247 276 132 822
Fragments arranged by size:
822 377 276 247 132
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 561 697 859 1854
Size: 561 136 162 995
Fragments arranged by size:
995 561 162 136
EcoNI CCTnn'n_nnAGG
Cuts at: 0 601 1255 1408 1854
Size: 601 654 153 446
Fragments arranged by size:
654 601 446 153
EcoO109I rG'GnC_Cy
Cuts at: 0 96 322 594 672 1023 1024 1268 1836
Size: 96 226 272 78 351 1 244 568
Cuts at: 1836 1854
Size: 18
Fragments arranged by size:
568 351 272 244 226 96 78 18
1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1308 1854
Size: 1308 546
FseI GG_CCGG'CC
Cuts at: 0 1061 1854
Size: 1061 793
FspI TGC'GCA
Cuts at: 0 16 1854
Size: 16 1838
FspAI rTGC'GCAy
Cuts at: 0 16 1854
Size: 16 1838
HaeII r_GCGC'y
Cuts at: 0 565 610 819 968 1018 1854
Size: 565 45 209 149 50 836
Fragments arranged by size:
836 565 209 149 50 45
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 352 384 844 876 1480 1512 1854
Size: 352 32 460 32 604 32 342
Fragments arranged by size:
604 460 352 342 32 32 32
KasI G'GCGC_C
Cuts at: 0 964 1854
Size: 964 890
MscI TGG'CCA
Cuts at: 0 1435 1854
Size: 1435 419
MslI CAynn'nnrTG
Cuts at: 0 343 1854
Size: 343 1511
MspA1I CmG'CkG
Cuts at: 0 130 1239 1316 1854
Size: 130 1109 77 538
Fragments arranged by size:
1109 538 130 77
NaeI GCC'GGC
Cuts at: 0 787 1059 1854
Size: 787 272 795
Fragments arranged by size:
795 787 272
NarI GG'CG_CC
Cuts at: 0 965 1854
Size: 965 889
NcoI C'CATG_G
Cuts at: 0 380 1715 1854
Size: 380 1335 139
Fragments arranged by size:
1335 380 139
NgoMIV G'CCGG_C
Cuts at: 0 785 1057 1854
Size: 785 272 797
Fragments arranged by size:
797 785 272
PasI CC'CwG_GG
Cuts at: 0 326 1019 1854
Size: 326 693 835
Fragments arranged by size:
835 693 326
PflMI CCAn_nnn'nTGG
Cuts at: 0 165 1536 1854
Size: 165 1371 318
Fragments arranged by size:
1371 318 165
PmlI CAC'GTG
Cuts at: 0 876 1854
Size: 876 978
PpuMI rG'GwC_Cy
Cuts at: 0 96 594 1836 1854
Size: 96 498 1242 18
Fragments arranged by size:
1242 498 96 18
PspOMI G'GGCC_C
Cuts at: 0 322 1023 1268 1854
Size: 322 701 245 586
Fragments arranged by size:
701 586 322 245
PstI C_TGCA'G
Cuts at: 0 892 1301 1854
Size: 892 409 553
Fragments arranged by size:
892 553 409
SacI G_AGCT'C
Cuts at: 0 379 626 902 1034 1854
Size: 379 247 276 132 820
Fragments arranged by size:
820 379 276 247 132
SacII CC_GC'GG
Cuts at: 0 131 1317 1854
Size: 131 1186 537
Fragments arranged by size:
1186 537 131
SapI GCTCTTCn'nnn_
Cuts at: 0 614 775 1840 1854
Size: 614 161 1065 14
Fragments arranged by size:
1065 614 161 14
SbfI CC_TGCA'GG
Cuts at: 0 1301 1854
Size: 1301 553
ScaI AGT'ACT
Cuts at: 0 1396 1854
Size: 1396 458
SfcI C'TryA_G
Cuts at: 0 825 888 1297 1830 1854
Size: 825 63 409 533 24
Fragments arranged by size:
825 533 409 63 24
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 670 1804 1854
Size: 670 1134 50
Fragments arranged by size:
1134 670 50
SfoI GGC'GCC
Cuts at: 0 966 1854
Size: 966 888
StuI AGG'CCT
Cuts at: 0 1303 1854
Size: 1303 551
StyI C'CwwG_G
Cuts at: 0 380 909 1128 1137 1571 1715 1854
Size: 380 529 219 9 434 144 139
Fragments arranged by size:
529 434 380 219 144 139 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 172 1717 1854
Size: 172 1545 137
Fragments arranged by size:
1545 172 137
TatI w'GTAC_w
Cuts at: 0 1394 1854
Size: 1394 460
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1028 1060 1721 1753 1854
Size: 1028 32 661 32 101
Fragments arranged by size:
1028 661 101 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 162 342 1016 1854
Size: 162 180 674 838
Fragments arranged by size:
838 674 180 162
ZraI GAC'GTC
Cuts at: 0 53 135 249 1116 1854
Size: 53 82 114 867 738
Fragments arranged by size:
867 738 114 82 53
Enzymes that do cut:
AatII AccI AcuI AhdI AlwNI ApaI AvaI AvrII
BamHI BanI BanII BbeI BbsI BbvCI BciVI BglI
Bme1580I BmgBI BmrI BpmI Bpu10I BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrFI BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI DraIII EaeI EagI EarI EcoICRI Eco57MI EcoNI
EcoO109I FalI FseI FspI FspAI HaeII Hin4I KasI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV PasI
PflMI PmlI PpuMI PspOMI PstI SacI SacII SapI
SbfI ScaI SfcI SfiI SfoI StuI StyI TaqII
TatI TstI XcmI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AfeI AflII AflIII AgeI AleI
AlfI AloI ApaLI ApoI AscI AseI AsiSI BaeI
BcgI BclI BfrBI BglII BlpI BmtI BplI BpuEI
BsiWI BsmI BsrDI BsrGI BssHII BstZ17I ClaI CspCI
DraI DrdI EciI EcoRI EcoRV HincII HindIII HpaI
KpnI MfeI MluI MmeI NdeI NheI NotI NruI
NsiI NspI PacI PciI PfoI PmeI PpiI PshAI
PsiI PspXI PsrI PvuI PvuII RsrII SalI SanDI
SexAI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI SspI SwaI Tth111I XbaI XhoI XmaI XmnI