################################## # NO CUT ENZYMES ( 164 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AfaI AflII AgeI AhdI Alw44I AlwI Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AviII AvrII BamHI BanI BbeI BbsI BcgI BciVI BclI BfaI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaBI BsaWI BseAI BsgI BsiEI BsiHKAI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspLU11I BsrDI BsrFI BsrGI BsrI BssHII BssSI Bst1107I BstBI BstEII BstF5I BstPI BstXI BstYI BstZ17I CelII Cfr10I ClaI CpoI DpnII DraI DraIII DrdI DsaI EagI Eam1105I EarI EclXI Eco47III Eco52I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FokI FseI FspI HincII HindII HindIII HpaI HphI KasI KpnI Ksp632I KspI MaeI MamI MboI MfeI MflI MluI MroI MseI MslI MunI NaeI NarI NcoI NdeI NdeII NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmeI PshAI PshBI Psp1406I PvuI RsaI RsrII SacI SacII SalI SapI Sau3AI ScaI SexAI SfiI SfoI SfuI SgrAI SmlI SnaBI SpeI SspBI SspI StuI StyI SwaI TaqI TfiI Tru9I Tsp509I Tth111I TthHB8I Van91I XbaI XcmI XhoI XhoII XmnI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### AcyI 106 GACGCC G[GA]^CG[CT]C AflIII 159 ACACGT A^C[GA][CT]GT AlwNI 137 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 271 CCTGAGG CC^T[ACGT]AGG ApaI 9 GGGCCC GGGCC^C BalI 123 TGGCCA TGG^CCA BbrPI 160 CACGTG CAC^GTG BbvCI 360 CCTCAGC CCTCAGC(-5/-2) BsaAI 160 CACGTG [CT]AC^GT[GA] BsaHI 106 GACGCC G[GA]^CG[CT]C BsaI 324 GGTCTC GGTCTC(1/5) BsmAI 325 GTCTC GTCTC(1/5) BspHI 86 TCATGA T^CATGA BspMI 116 ACCTGC ACCTGC(4/8) BsrBI 298 GAGCGG CCGCTC(-3/-3) BstAPI 234 GCATTTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC Bsu36I 271 CCTGAGG CC^T[ACGT]AGG EaeI 123 TGGCCA [CT]^GGCC[GA] Eco81I 271 CCTGAGG CC^T[ACGT]AGG EcoNI 240 CCTGCAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG HaeII 48 GGCGCT [GA]GCGC^[CT] HapII 111 CCGG C^CGG HgaI 106 GACGC GACGC(5/10) Hin1I 106 GACGCC G[GA]^CG[CT]C HinfI 133 GAGTC G^A[ACGT]TC HpaII 111 CCGG C^CGG MaeII 161 ACGT A^CGT MaeIII 309 GTCAC ^GT[ACGT]AC MluNI 123 TGGCCA TGG^CCA MscI 123 TGGCCA TGG^CCA MspA1I 140 CAGCTG C[AC]G^C[GT]G MspI 111 CCGG C^CGG NspI 365 GCATGC [GA]CATG^[CT] PleI 133 GAGTC GAGTC(4/5) PmaCI 160 CACGTG CAC^GTG PmlI 160 CACGTG CAC^GTG PpuMI 113 GGGACCT [GA]G^G[AT]CC[CT] PvuII 140 CAGCTG CAG^CTG RcaI 86 TCATGA T^CATGA SbfI 240 CCTGCAGG CCTGCA^GG SfaNI 345 GCATC GCATC(5/9) SmaI 110 CCCGGG CCC^GGG SphI 365 GCATGC GCATG^C Sse8387I 240 CCTGCAGG CCTGCA^GG Tsp45I 309 GTCAC ^GT[GC]AC XmaCI 110 CCCGGG C^CCGGG XmaI 110 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AccII 35 CGCG CG^CG 55 CGCG AvaII 114 GGACC G^G[AT]CC 168 GGACC BanII 9 GGGCCC G[GA]GC[CT]^C 265 GGGCTC BcnI 110 CCCGG CC^[GC]GG 111 CCGGG BmyI 9 GGGCCC G[AGT]GC[ACT]^C 265 GGGCTC BpmI 39 CTGGAG CTGGAG(16/14) 334 CTGGAG BsaJI 110 CCCGGG C^C[ACGT][ACGT]GG 354 CCTGGG BsiYI 240 CCTGCAGGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 293 CCACTGAGCGG BslI 240 CCTGCAGGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 293 CCACTGAGCGG BsmFI 113 GGGAC GGGAC(10/14) 156 GGGAC Bsp1286I 9 GGGCCC G[AGT]GC[ACT]^C 265 GGGCTC BstNI 126 CCAGG CC^[AT]GG 354 CCTGG BstUI 35 CGCG CG^CG 55 CGCG DraII 9 GGGCCCC [GA]G^G[ACGT]CC[CT] 113 GGGACCT EcoO109I 9 GGGCCCC [GA]G^G[ACGT]CC[CT] 113 GGGACCT EcoRII 126 CCAGG ^CC[AT]GG 354 CCTGG FauI 7 GCGGG CCCGC(4/6) 207 GCGGG MboII 177 GAAGA GAAGA(8/7) 339 GAAGA MvaI 126 CCAGG CC^[AT]GG 354 CCTGG MvnI 35 CGCG CG^CG 55 CGCG NciI 110 CCCGG CC^[GC]GG 111 CCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 26 enzymes) # ######################################### AciI AluI AvaI BbvI BseRI BsoBI BssKI Cac8I CfoI Cfr13I DdeI Fnu4HI HaeIII HhaI HinP1I ItaI MnlI MwoI NlaIII NlaIV PstI Sau96I ScrFI SfcI TseI TspRI