################################## # NO CUT ENZYMES ( 135 enzymes) # ################################## AatII Acc65I AccIII AclI AcyI AflII AflIII AhdI Alw44I ApaI ApaLI AscI Asp700I Asp718I AspEI AspI AvaII AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbvCI BcgI BcnI BfrI BglI BglII BlnI BpmI BsaAI BsaBI BsaHI BsaI BseAI BsiEI BsiWI BsmAI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrGI BssSI Bst1107I BstEII BstPI BstXI BstZ17I ClaI CpoI DraI DraII DrdI EaeI EagI Eam1105I EclXI Eco52I EcoO109I EcoO65I EcoRI EcoRV EcoT22I FauI FseI FspI HgaI Hin1I HincII HindII HpaI KasI KpnI KspI MamI MfeI MluI MluNI MroI MscI MunI NaeI NarI NciI NgoMIV NheI NotI NruI NsiI PacI PflFI PflMI PleI PmaCI PmeI PmlI PpuMI PshAI Psp1406I PstI PvuI RcaI RsrII SacI SacII SalI SbfI SexAI SfiI SfoI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI StuI SwaI Tth111I Van91I XbaI XcmI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 61 enzymes) # ################################### AccI 794 GTAGAC GT^[AC][GT]AC AccII 1669 CGCG CG^CG AgeI 946 ACCGGT A^CCGGT AlwNI 1736 CAGTTACTG CAG[ACGT][ACGT][ACGT]^CTG AocI 1656 CCTAAGG CC^T[ACGT]AGG Aor51HI 612 AGCGCT AGC^GCT AseI 1609 ATTAAT AT^TAAT AsnI 1609 ATTAAT AT^TAAT AspHI 1259 GTGCTC G[AT]GC[AT]^C AvaI 1307 CTCGAG C^[CT]CG[GA]G BciVI 20 GTATCC GTATCC(6/5) BclI 183 TGATCA T^GATCA BlpI 1261 GCTCAGC GC^T[ACGT]AGC BmyI 1259 GTGCTC G[AGT]GC[ACT]^C Bpu1102I 1261 GCTCAGC GC^T[ACGT]AGC BsaWI 946 ACCGGT [AT]^CCGG[AT] BseRI 8 CTCCTC GAGGAG(10/8) BsiHKAI 1259 GTGCTC G[AT]GC[AT]^C BsiYI 1857 CCTAATTCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 1857 CCTAATTCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmFI 1769 GGGAC GGGAC(10/14) BsoBI 1307 CTCGAG C^[CT]CG[GA]G Bsp1286I 1259 GTGCTC G[AGT]GC[ACT]^C BsrFI 946 ACCGGT [GA]^CCGG[CT] BssHII 1668 GCGCGC G^CGCGC BstBI 446 TTCGAA TT^CGAA BstUI 1669 CGCG CG^CG Bsu36I 1656 CCTAAGG CC^T[ACGT]AGG CelII 1261 GCTCAGC GC^T[ACGT]AGC Cfr10I 946 ACCGGT [GA]^CCGG[CT] Cfr13I 1544 GGGCC G^G[ACGT]CC DraIII 1586 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 104 CCATGG C^C[GA][CT]GG Eco47III 612 AGCGCT AGC^GCT Eco81I 1656 CCTAAGG CC^T[ACGT]AGG EcoNI 1857 CCTAATTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoT14I 104 CCATGG C^C[AT][AT]GG FbaI 183 TGATCA T^GATCA HaeII 612 AGCGCT [GA]GCGC^[CT] HaeIII 1545 GGCC GG^CC HapII 947 CCGG C^CGG HpaII 947 CCGG C^CGG MaeII 747 ACGT A^CGT MslI 1924 CACCTGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 839 CAGCTG C[AC]G^C[GT]G MspI 947 CCGG C^CGG MvnI 1669 CGCG CG^CG NcoI 104 CCATGG C^CATGG NdeI 1999 CATATG CA^TATG NlaIV 1089 GGCTCC GG[ACGT]^[ACGT]CC NspV 446 TTCGAA TT^CGAA PaeR7I 1307 CTCGAG C^TCGAG PinAI 946 ACCGGT A^CCGGT PshBI 1609 ATTAAT AT^TAAT PvuII 839 CAGCTG CAG^CTG SapI 781 GAAGAGC GCTCTTC(1/4) Sau96I 1544 GGGCC G^G[ACGT]CC ScaI 736 AGTACT AGT^ACT SfuI 446 TTCGAA TT^CGAA StyI 104 CCATGG C^C[AT][AT]GG XhoI 1307 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AciI 681 GCGG CCGC(-3/-1) 2040 GCGG AlwI 108 GGATC GGATC(4/5) 759 GGATC BbsI 449 GAAGAC GAAGAC(2/6) 907 GAAGAC BpuAI 449 GAAGAC GAAGAC(2/6) 907 GAAGAC BsaJI 104 CCATGG C^C[ACGT][ACGT]GG 263 CCAGGG BsrDI 98 CATTGC GCAATG(2/0) 1878 CATTGC BsrI 1134 ACTGG ACTGG(1/-1) 1103 CCAGT BssKI 263 CCAGG ^CC[ACGT]GG 1179 CCAGG BstF5I 683 GGATG GGATG(2/0) 1047 GGATG BstNI 263 CCAGG CC^[AT]GG 1179 CCAGG BstYI 108 GGATCT [GA]^GATC[CT] 759 GGATCT Cac8I 1668 GCGCGC GC[ACGT]^[ACGT]GC 1887 GCGTGC EarI 11 CTCTTC CTCTTC(1/4) 781 GAAGAG EcoRII 263 CCAGG ^CC[AT]GG 1179 CCAGG FokI 683 GGATG GGATG(9/13) 1047 GGATG HindIII 442 AAGCTT A^AGCTT 495 AAGCTT Ksp632I 11 CTCTTC CTCTTC(1/4) 781 GAAGAG MflI 108 GGATCT [GA]^GATC[CT] 759 GGATCT MvaI 263 CCAGG CC^[AT]GG 1179 CCAGG NspI 468 ACATGC [GA]CATG^[CT] 1128 ACATGC ScrFI 263 CCAGG CC^[ACGT]GG 1179 CCAGG SmlI 1307 CTCGAG C^T[CT][GA]AG 1805 CTCAAG XhoII 108 GGATCT [GA]^GATC[CT] 759 GGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 39 enzymes) # ######################################### AcsI AfaI AluI ApoI BbvI BfaI BsgI BstAPI CfoI DdeI DpnII Fnu4HI HhaI HinP1I HinfI HphI ItaI MaeI MaeIII MboI MboII MnlI MseI MwoI NdeII NlaIII RsaI Sau3AI SfaNI SfcI SspI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I