(Circular) (Six-base) MAPSORT of: pFN21AE4192.seq Check: 5451 from: 1 to: 6784 pFN21AE4192 6784 bp With 182 enzymes: SgfI * March 28, 2012 13:06 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4594 278 Size: 53 83 186 936 3058 2468 Fragments arranged by size: 3058 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2800 4127 1937 Size: 863 1327 4594 Fragments arranged by size: 4594 1327 863 Acc65I G'GTAC_C Cuts at: 4105 4105 Size: 6784 AclI AA'CG_TT Cuts at: 1563 4911 5284 1563 Size: 3348 373 3063 Fragments arranged by size: 3348 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2220 4847 6231 6499 1632 Size: 588 2627 1384 268 1917 Fragments arranged by size: 2627 1917 1384 588 268 AfeI AGC'GCT Cuts at: 2619 2619 Size: 6784 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6587 AflIII A'CryG_T Cuts at: 1203 5683 6660 1203 Size: 4480 977 1327 Fragments arranged by size: 4480 1327 977 AgeI A'CCGG_T Cuts at: 2951 5615 2951 Size: 2664 4120 AhdI GACnn_n'nnGTC Cuts at: 5513 5513 Size: 6784 AleI CACnn'nnGTG Cuts at: 1166 3933 1166 Size: 2767 4017 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2961 1184 Size: 1777 5007 AlwNI CAG_nnn'CTG Cuts at: 3746 4527 6099 3746 Size: 781 1572 4431 Fragments arranged by size: 4431 1572 781 ApaLI G'TGCA_C Cuts at: 4841 5997 4841 Size: 1156 5628 ApoI r'AATT_y Cuts at: 1493 2276 2415 2808 3243 3403 3542 3823 4093 Size: 783 139 393 435 160 139 281 270 Cuts at: 4093 4319 4471 6580 1493 Size: 226 152 2109 1697 Fragments arranged by size: 2109 1697 783 435 393 281 270 226 160 152 139 139 AseI AT'TA_AT Cuts at: 160 1032 3615 5338 160 Size: 872 2583 1723 1606 Fragments arranged by size: 2583 1723 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6784 AvaI C'yCGr_G Cuts at: 1870 1943 3312 4109 1870 Size: 73 1369 797 4545 Fragments arranged by size: 4545 1369 797 73 BamHI G'GATC_C Cuts at: 4114 4481 4114 Size: 367 6417 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4105 5560 618 Size: 325 217 627 2318 1455 1842 Fragments arranged by size: 2318 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 4103 1391 Size: 2712 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2461 2919 928 Size: 971 562 458 4793 Fragments arranged by size: 4793 971 562 458 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4975 5009 1903 Size: 34 3038 34 3678 Fragments arranged by size: 3678 3038 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2036 4676 5886 2036 Size: 2640 1210 2934 Fragments arranged by size: 2934 2640 1210 BclI T'GATC_A Cuts at: 1548 2188 1548 Size: 640 6144 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5395 136 Size: 107 122 71 631 605 3723 1525 Fragments arranged by size: 3723 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6779 6779 Size: 6784 BlpI GC'TnA_GC Cuts at: 3267 4201 3267 Size: 934 5850 Bme1580I G_kGCm'C Cuts at: 1790 4845 6001 1790 Size: 3055 1156 2573 Fragments arranged by size: 3055 2573 1156 BmgBI CAC'GTC Cuts at: 1318 2753 1318 Size: 1435 5349 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4515 5473 442 Size: 416 53 83 399 24 3098 958 1753 Fragments arranged by size: 3098 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6784 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 5444 1239 Size: 4205 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3795 4909 5774 6072 3795 Size: 1114 865 298 4507 Fragments arranged by size: 4507 1114 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5447 882 Size: 617 3948 2219 Fragments arranged by size: 3948 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4575 493 Size: 711 3371 2702 Fragments arranged by size: 3371 2702 711 BsaBI GATnn'nnATC Cuts at: 6778 6778 Size: 6784 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4591 4973 5608 275 Size: 53 83 186 936 3058 382 635 1451 Fragments arranged by size: 3058 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2951 5217 5615 5889 6036 6447 2951 Size: 2266 398 274 147 411 3288 Fragments arranged by size: 3288 2266 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6559 Fragments arranged by size: 6559 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2004 1186 Size: 818 5966 BseYI C'CCAG_C Cuts at: 5987 6483 5987 Size: 496 6288 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3674 3915 3981 1163 Size: 573 1938 241 66 3966 Fragments arranged by size: 3966 1938 573 241 66 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4234 4995 6023 1540 Size: 275 186 2233 761 1028 2301 Fragments arranged by size: 2301 2233 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3268 4845 4930 6001 3268 Size: 1577 85 1071 4051 Fragments arranged by size: 4051 1577 1071 85 BsmI GAATG_Cn' Cuts at: 4295 4388 4295 Size: 93 6691 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3268 4103 4845 4930 6001 1391 Size: 399 1478 835 742 85 1071 2174 Fragments arranged by size: 2174 1478 1071 835 742 399 85 BspEI T'CCGG_A Cuts at: 6447 6447 Size: 6784 BspHI T'CATG_A Cuts at: 4673 6403 4673 Size: 1730 5054 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4139 844 Size: 3295 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4671 1394 Size: 429 2848 3507 Fragments arranged by size: 3507 2848 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2102 3882 5279 5453 65 Size: 1183 854 1780 1397 174 1396 Fragments arranged by size: 1780 1397 1396 1183 854 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2951 5428 5615 1700 Size: 110 1141 2477 187 2869 Fragments arranged by size: 2869 2477 1141 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6784 BssHII G'CGCG_C Cuts at: 1925 3673 1925 Size: 1748 5036 BssSI C'ACGA_G Cuts at: 4844 5856 4844 Size: 1012 5772 BstAPI GCAn_nnn'nTGC Cuts at: 794 2390 3434 3893 794 Size: 1596 1044 459 3685 Fragments arranged by size: 3685 1596 1044 459 BstBI TT'CG_AA Cuts at: 2452 5588 2452 Size: 3136 3648 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6784 BstYI r'GATC_y Cuts at: 1972 2113 2764 4114 4481 4866 4883 6335 6421 Size: 141 651 1350 367 385 17 1452 86 Cuts at: 6421 6433 6779 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1350 651 385 367 346 141 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3662 3662 Size: 6784 BtgI C'CryG_G Cuts at: 513 1065 2109 513 Size: 552 1044 5188 Fragments arranged by size: 5188 1044 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2829 487 Size: 683 56 1603 4442 Fragments arranged by size: 4442 1603 683 56 BtsI GCAGTG_nn' Cuts at: 2825 3054 3115 3649 3903 4300 5094 5114 2825 Size: 229 61 534 254 397 794 20 4495 Fragments arranged by size: 4495 794 534 397 254 229 61 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6784 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6749 DraI TTT'AAA Cuts at: 4088 4088 Size: 6784 DraIII CAC_nnn'GTG Cuts at: 3596 3596 Size: 6784 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5791 809 Size: 507 4475 1802 Fragments arranged by size: 4475 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4231 5120 6749 Size: 54 1425 50 275 18 2401 889 1629 Cuts at: 6749 8 Size: 43 Fragments arranged by size: 2401 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4231 1537 Size: 275 2419 4090 Fragments arranged by size: 4090 2419 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2022 2781 4714 1353 Size: 669 759 1933 3423 Fragments arranged by size: 3423 1933 759 669 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5371 5745 5891 387 Size: 1064 138 3782 374 146 1280 Fragments arranged by size: 3782 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2220 4847 5444 6231 6499 1239 Size: 393 588 2627 597 787 268 1524 Fragments arranged by size: 2627 1524 787 597 588 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 3866 3866 Size: 6784 EcoRI G'AATT_C Cuts at: 4093 4471 4093 Size: 378 6406 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2153 2279 2807 2153 Size: 126 528 6130 Fragments arranged by size: 6130 528 126 FspI TGC'GCA Cuts at: 5290 5290 Size: 6784 HaeII r_GCGC'y Cuts at: 1678 2621 5931 1678 Size: 943 3310 2531 Fragments arranged by size: 3310 2531 943 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 1999 2031 3503 3535 3785 Size: 32 135 32 427 32 1472 32 250 Cuts at: 3785 3817 5425 5457 5499 5531 1373 Size: 32 1608 32 42 32 2626 Fragments arranged by size: 2626 1608 1472 427 250 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4128 4374 669 Size: 988 281 2190 246 3079 Fragments arranged by size: 3079 2190 988 281 246 HindIII A'AGCT_T Cuts at: 748 2447 2500 748 Size: 1699 53 5032 Fragments arranged by size: 5032 1699 53 HpaI GTT'AAC Cuts at: 4374 4374 Size: 6784 KpnI G_GTAC'C Cuts at: 4109 4109 Size: 6784 MfeI C'AATT_G Cuts at: 4383 4383 Size: 6784 MluI A'CGCG_T Cuts at: 6660 6660 Size: 6784 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2179 2316 4039 5898 6082 1305 Size: 874 137 1723 1859 184 2007 Fragments arranged by size: 2007 1859 1723 874 184 137 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4962 Fragments arranged by size: 4962 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3933 4742 5101 5260 518 Size: 648 2767 809 359 159 2042 Fragments arranged by size: 2767 2042 809 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2846 4200 4877 6025 6270 1606 Size: 1240 1354 677 1148 245 2120 Fragments arranged by size: 2120 1354 1240 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2109 513 Size: 552 1044 5188 Fragments arranged by size: 5188 1044 552 NdeI CA'TA_TG Cuts at: 387 4005 387 Size: 3618 3166 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6784 NotI GC'GGCC_GC Cuts at: 4231 4231 Size: 6784 NspI r_CATG'y Cuts at: 1132 1229 2477 3137 4142 5687 1132 Size: 97 1248 660 1005 1545 2229 Fragments arranged by size: 2229 1545 1248 1005 660 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6784 PciI A'CATG_T Cuts at: 5683 5683 Size: 6784 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6784 PmeI GTTT'AAAC Cuts at: 4088 4088 Size: 6784 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3306 3338 4853 4885 6366 6398 3306 Size: 32 1515 32 1481 32 3692 Fragments arranged by size: 3692 1515 1481 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6784 PsiI TTA'TAA Cuts at: 2037 4354 2037 Size: 2317 4467 PstI C_TGCA'G Cuts at: 830 4136 830 Size: 3306 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6784 PvuII CAG'CTG Cuts at: 2846 2846 Size: 6784 SalI G'TCGA_C Cuts at: 1936 4126 1936 Size: 2190 4594 SapI GCTCTTCn'nnn_ Cuts at: 2781 2781 Size: 6784 SbfI CC_TGCA'GG Cuts at: 4136 4136 Size: 6784 ScaI AGT'ACT Cuts at: 2743 5032 6744 2743 Size: 2289 1712 2783 Fragments arranged by size: 2783 2289 1712 SfcI C'TryA_G Cuts at: 826 1045 2583 3558 3621 4132 5267 5948 6139 Size: 219 1538 975 63 511 1135 681 191 Cuts at: 6139 826 Size: 1471 Fragments arranged by size: 1538 1471 1135 975 681 511 219 191 63 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6784 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6784 SmaI CCC'GGG Cuts at: 1872 4111 1872 Size: 2239 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3312 3810 4888 5789 6051 820 Size: 197 926 1369 498 1078 901 262 1553 Fragments arranged by size: 1553 1369 1078 926 901 498 262 197 SnaBI TAC'GTA Cuts at: 493 4575 493 Size: 4082 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6784 SphI G_CATG'C Cuts at: 1132 4142 1132 Size: 3010 3774 SspI AAT'ATT Cuts at: 5 52 2205 2964 4027 4039 4708 5 Size: 47 2153 759 1063 12 669 2081 Fragments arranged by size: 2153 2081 1063 759 669 47 12 StuI AGG'CCT Cuts at: 1517 1517 Size: 6784 StyI C'CwwG_G Cuts at: 513 1065 2109 4223 513 Size: 552 1044 2114 3074 Fragments arranged by size: 3074 2114 1044 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3251 4811 4828 4981 5166 5611 325 Size: 1255 1671 1560 17 153 185 445 1498 Fragments arranged by size: 1671 1560 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2725 2741 3190 Size: 274 80 33 51 1443 748 16 449 Cuts at: 3190 3904 5030 6742 96 Size: 714 1126 1712 138 Fragments arranged by size: 1712 1443 1126 748 714 449 274 138 80 51 33 16 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2850 2882 3474 3506 6744 6776 2850 Size: 32 592 32 3238 32 2858 Fragments arranged by size: 3238 2858 592 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6784 XbaI T'CTAG_A Cuts at: 4120 4120 Size: 6784 XhoI C'TCGA_G Cuts at: 1943 3312 1943 Size: 1369 5415 XmaI C'CCGG_G Cuts at: 1870 4109 1870 Size: 2239 4545 XmnI GAAnn'nnTTC Cuts at: 4913 4913 Size: 6784 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4592 276 Size: 53 83 186 936 3058 2468 Fragments arranged by size: 3058 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I EcoICRI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI