Restriction map for
pFN21AE4180
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# NO CUT ENZYMES ( 104 enzymes) #
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Acc65I AccI AclI AflII AgeI
AhdI Alw44I AocI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AviII AvrII BamHI BbeI BcgI
BciVI BclI BfaI BfrI BglII
BlnI BsaBI BsiWI BsmI Bsp1407I
BspDI BspHI BspLU11I BsrGI BssHII
Bst1107I BstBI BstZ17I Bsu36I ClaI
CpoI DraI DrdI EagI Eam1105I
EclXI Eco52I Eco81I EcoRV EcoT22I
FbaI FseI FspI HincII HindII
HindIII HpaI KasI KpnI KspI
MaeI MamI MfeI MluI MseI
MunI NarI NdeI NheI NotI
NruI NsiI NspI NspV PacI
PinAI PmeI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI SapI ScaI SexAI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI SspBI SspI SwaI Tru9I
XbaI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 33 enzymes) #
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AccIII 68 TCCGGA T^CCGGA
AfaI 1002 GTAC GT^AC
AflIII 1727 ACGTGT A^C[GA][CT]GT
Aor51HI 1079 AGCGCT AGC^GCT
AspI 706 GACGACGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbsI 1462 GTCTTC GAAGAC(2/6)
BbvCI 549 CCTCAGC CCTCAGC(-5/-2)
BlpI 1674 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1674 GCTCAGC GC^T[ACGT]AGC
BpuAI 1462 GTCTTC GAAGAC(2/6)
BsaWI 68 TCCGGA [AT]^CCGG[AT]
BseAI 68 TCCGGA T^CCGGA
BsiEI 51 CGACCG CG[GA][CT]^CG
BsmBI 178 GAGACG CGTCTC(1/5)
BspEI 68 TCCGGA T^CCGGA
BspMI 209 ACCTGC ACCTGC(4/8)
BsrDI 1641 GCAATG GCAATG(2/0)
BssSI 1210 CACGAG CACGAG(-5/-1)
BstEII 1842 GGTGACC G^GT[ACGT]ACC
BstPI 1842 GGTGACC G^GT[ACGT]ACC
CelII 1674 GCTCAGC GC^T[ACGT]AGC
Eco47III 1079 AGCGCT AGC^GCT
EcoO65I 1842 GGTGACC G^GT[ACGT]ACC
MroI 68 TCCGGA T^CCGGA
NcoI 1853 CCATGG C^CATGG
PflFI 706 GACGACGTC GAC[ACGT]^[ACGT][ACGT]GTC
RsaI 1002 GTAC GT^AC
SbfI 1785 CCTGCAGG CCTGCA^GG
SfiI 1105 GGCCTACGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
Sse8387I 1785 CCTGCAGG CCTGCA^GG
StuI 1104 AGGCCT AGG^CCT
TfiI 411 GAATC G^A[AT]TC
Tth111I 706 GACGACGTC GAC[ACGT]^[ACGT][ACGT]GTC
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# TWO CUT ENZYMES ( 27 enzymes) #
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AatII 709 GACGTC GACGT^C
1741 GACGTC
AcsI 1288 GAATTC [GA]^AATT[CT]
1456 GAATTC
ApoI 1288 GAATTC [GA]^AATT[CT]
1456 GAATTC
BbrPI 942 CACGTG CAC^GTG
1132 CACGTG
BsaAI 942 CACGTG [CT]AC^GT[GA]
1132 CACGTG
BsaI 874 GAGACC GGTCTC(1/5)
1213 GAGACC
BsgI 826 CTGCAC GTGCAG(16/14)
1129 CTGCAC
BsrBI 1043 CCGCTC CCGCTC(-3/-3)
1545 GAGCGG
BstAPI 828 GCACTTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1556 GCACCATCTGC
BstXI 1652 CCACCGAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1847 CCAAGACCATGG
BstYI 65 AGATCC [GA]^GATC[CT]
1802 AGATCC
DraIII 561 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
1188 CACCCTGTG
EarI 1222 CTCTTC CTCTTC(1/4)
475 GAAGAG
EcoNI 778 CCTGTGGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1830 CCTTGGCCAGG
EcoRI 1288 GAATTC G^AATTC
1456 GAATTC
HaeII 1079 AGCGCT [GA]GCGC^[CT]
1678 AGCGCC
Ksp632I 1222 CTCTTC CTCTTC(1/4)
475 GAAGAG
MflI 65 AGATCC [GA]^GATC[CT]
1802 AGATCC
NlaIII 456 CATG CATG^
1854 CATG
PaeR7I 277 CTCGAG C^TCGAG
871 CTCGAG
PflMI 649 CCAGAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
930 CCAGACCGTGG
PmaCI 942 CACGTG CAC^GTG
1132 CACGTG
PmlI 942 CACGTG CAC^GTG
1132 CACGTG
SmlI 277 CTCGAG C^T[CT][GA]AG
871 CTCGAG
Van91I 649 CCAGAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
930 CCAGACCGTGG
XhoI 277 CTCGAG C^TCGAG
871 CTCGAG
XhoII 65 AGATCC [GA]^GATC[CT]
1802 AGATCC
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# 3 OR MORE CUT ENZYMES ( 94 enzymes) #
#########################################
AccII AciI AcyI AluI AlwI
AlwNI ApaI AspHI AvaI AvaII
BalI BanI BanII BbvI BcnI
BglI BmyI BpmI BsaHI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BsrFI BsrI
BssKI BstF5I BstNI BstUI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI MaeII
MaeIII MboI MboII MluNI MnlI
MscI MslI MspA1I MspI MvaI
MvnI MwoI NaeI NciI NdeII
NgoMIV NlaIV PleI PpuMI PstI
PvuII Sau3AI Sau96I ScrFI SfaNI
SfcI StyI TaqI TseI Tsp45I
Tsp509I TspRI TthHB8I XcmI