(Circular) (Six-base) MAPSORT of: pFN21AE4180.seq Check: 7114 from: 1 to: 6646 pFN21AE4180 6646 bp With 182 enzymes: SgfI * March 28, 2012 13:06 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2223 2223 Size: 6646 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2718 3750 4456 278 Size: 53 83 186 936 1182 1032 706 2468 Fragments arranged by size: 2468 1182 1032 936 706 186 83 53 AccI GT'mk_AC Cuts at: 1937 3989 1937 Size: 2052 4594 Acc65I G'GTAC_C Cuts at: 3967 3967 Size: 6646 AclI AA'CG_TT Cuts at: 1563 4773 5146 1563 Size: 3210 373 3063 Fragments arranged by size: 3210 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2499 3187 3782 3833 4709 6093 6361 1632 Size: 867 688 595 51 876 1384 268 1917 Fragments arranged by size: 1917 1384 876 867 688 595 268 51 AfeI AGC'GCT Cuts at: 3086 3086 Size: 6646 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6449 AflIII A'CryG_T Cuts at: 1203 3732 5545 6522 1203 Size: 2529 1813 977 1327 Fragments arranged by size: 2529 1813 1327 977 AgeI A'CCGG_T Cuts at: 5477 5477 Size: 6646 AhdI GACnn_n'nnGTC Cuts at: 5375 5375 Size: 6646 AleI CACnn'nnGTG Cuts at: 1166 2217 3141 3872 1166 Size: 1051 924 731 3940 Fragments arranged by size: 3940 1051 924 731 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3159 1184 Size: 1975 4671 AlwNI CAG_nnn'CTG Cuts at: 2660 3687 3811 4389 5961 2660 Size: 1027 124 578 1572 3345 Fragments arranged by size: 3345 1572 1027 578 124 ApaI G_GGCC'C Cuts at: 2012 2547 3835 2012 Size: 535 1288 4823 Fragments arranged by size: 4823 1288 535 ApaLI G'TGCA_C Cuts at: 4703 5859 4703 Size: 1156 5490 ApoI r'AATT_y Cuts at: 1493 3293 3461 3955 4181 4333 6442 1493 Size: 1800 168 494 226 152 2109 1697 Fragments arranged by size: 2109 1800 1697 494 226 168 152 AseI AT'TA_AT Cuts at: 160 1032 5200 160 Size: 872 4168 1606 Fragments arranged by size: 4168 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6646 AvaI C'yCGr_G Cuts at: 1870 1943 2282 2546 2876 3971 1870 Size: 73 339 264 330 1095 4545 Fragments arranged by size: 4545 1095 339 330 264 73 BamHI G'GATC_C Cuts at: 3976 4343 3976 Size: 367 6279 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3013 3274 3560 3967 5422 Size: 325 217 627 1226 261 286 407 1455 Cuts at: 5422 618 Size: 1842 Fragments arranged by size: 1842 1455 1226 627 407 325 286 261 217 BanII G_rGCy'C Cuts at: 1391 2012 2547 2829 3175 3482 3835 3965 1391 Size: 621 535 282 346 307 353 130 4072 Fragments arranged by size: 4072 621 535 353 346 307 282 130 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3460 928 Size: 971 1561 4114 Fragments arranged by size: 4114 1561 971 BbvCI CC'TCA_GC Cuts at: 2555 2555 Size: 6646 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4837 4871 1903 Size: 34 2900 34 3678 Fragments arranged by size: 3678 2900 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4538 5748 4538 Size: 1210 5436 BclI T'GATC_A Cuts at: 1548 1548 Size: 6646 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2301 2690 2988 Size: 107 122 71 631 605 629 389 298 Cuts at: 2988 3117 3794 5257 136 Size: 129 677 1463 1525 Fragments arranged by size: 1525 1463 677 631 629 605 389 298 129 122 107 71 BglII A'GATC_T Cuts at: 6641 6641 Size: 6646 BlpI GC'TnA_GC Cuts at: 3680 4063 3680 Size: 383 6263 Bme1580I G_kGCm'C Cuts at: 1790 2012 2547 3279 3563 3835 4707 5863 1790 Size: 222 535 732 284 272 872 1156 2573 Fragments arranged by size: 2573 1156 872 732 535 284 272 222 BmgBI CAC'GTC Cuts at: 1318 3418 3591 1318 Size: 2100 173 4373 Fragments arranged by size: 4373 2100 173 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2458 2480 2563 Size: 416 53 83 399 24 1041 22 83 Cuts at: 2563 4377 5335 442 Size: 1814 958 1753 Fragments arranged by size: 1814 1753 1041 958 416 399 83 83 53 24 22 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6646 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2201 2318 2682 2843 3109 3601 5306 1239 Size: 962 117 364 161 266 492 1705 2579 Fragments arranged by size: 2579 1705 962 492 364 266 161 117 Bpu10I CC'TnA_GC Cuts at: 2555 2555 Size: 6646 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4771 5636 5934 4771 Size: 865 298 5483 Fragments arranged by size: 5483 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2873 3212 5309 882 Size: 617 1374 339 2097 2219 Fragments arranged by size: 2219 2097 1374 617 339 BsaAI yAC'GTr Cuts at: 493 1204 2949 3139 4437 493 Size: 711 1745 190 1298 2702 Fragments arranged by size: 2702 1745 1298 711 190 BsaBI GATnn'nnATC Cuts at: 6640 6640 Size: 6646 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2044 2715 3372 3747 Size: 53 83 186 936 511 671 657 375 Cuts at: 3747 4453 4835 5470 275 Size: 706 382 635 1451 Fragments arranged by size: 1451 936 706 671 657 635 511 382 375 186 83 53 BsaWI w'CCGG_w Cuts at: 2073 5079 5477 5751 5898 6309 2073 Size: 3006 398 274 147 411 2410 Fragments arranged by size: 3006 2410 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2951 2981 3436 3466 1178 Size: 30 165 30 1548 30 455 30 4358 Fragments arranged by size: 4358 1548 455 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2498 2609 2933 3038 3457 3737 1186 Size: 1312 111 324 105 419 280 4095 Fragments arranged by size: 4095 1312 419 324 280 111 105 BseYI C'CCAG_C Cuts at: 2062 2245 2735 2883 3411 3822 5849 6345 2062 Size: 183 490 148 528 411 2027 496 2363 Fragments arranged by size: 2363 2027 528 496 490 411 183 148 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2816 3119 1163 Size: 573 1080 303 4690 Fragments arranged by size: 4690 1080 573 303 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2059 4096 4857 5885 1540 Size: 275 186 58 2037 761 1028 2301 Fragments arranged by size: 2301 2037 1028 761 275 186 58 BsiHKAI G_wGCw'C Cuts at: 2115 3000 3711 4707 4792 5863 2115 Size: 885 711 996 85 1071 2898 Fragments arranged by size: 2898 1071 996 885 711 85 BsmI GAATG_Cn' Cuts at: 4157 4250 4157 Size: 93 6553 BsmBI CGTCTCn'nnnn_ Cuts at: 2177 2177 Size: 6646 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2012 2115 2547 2829 3000 3175 3279 Size: 399 222 103 432 282 171 175 104 Cuts at: 3279 3482 3563 3711 3835 3965 4707 4792 5863 Size: 203 81 148 124 130 742 85 1071 Cuts at: 5863 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 432 399 282 222 203 175 171 148 130 124 104 103 85 81 BspEI T'CCGG_A Cuts at: 2073 6309 2073 Size: 4236 2410 BspHI T'CATG_A Cuts at: 4535 6265 4535 Size: 1730 4916 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2223 4001 844 Size: 1379 1778 3489 Fragments arranged by size: 3489 1778 1379 BsrBI CCG'CTC Cuts at: 1394 1823 3050 3552 4533 1394 Size: 429 1227 502 981 3507 Fragments arranged by size: 3507 1227 981 502 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3653 5141 5315 65 Size: 1183 2405 1488 174 1396 Fragments arranged by size: 2405 1488 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2058 2908 3146 3386 3781 5290 5477 Size: 110 248 850 238 240 395 1509 187 Cuts at: 5477 1700 Size: 2869 Fragments arranged by size: 2869 1509 850 395 248 240 238 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6646 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6646 BssSI C'ACGA_G Cuts at: 3215 4706 5718 3215 Size: 1491 1012 4143 Fragments arranged by size: 4143 1491 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2839 3567 794 Size: 2045 728 3873 Fragments arranged by size: 3873 2045 728 BstBI TT'CG_AA Cuts at: 5450 5450 Size: 6646 BstEII G'GTnAC_C Cuts at: 1184 3847 1184 Size: 2663 3983 BstXI CCAn_nnnn'nTGG Cuts at: 3664 3859 3664 Size: 195 6451 BstYI r'GATC_y Cuts at: 1972 2070 3807 3976 4343 4728 4745 6197 6283 Size: 98 1737 169 367 385 17 1452 86 Cuts at: 6283 6295 6641 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1737 1452 385 367 346 169 98 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2940 3615 3858 513 Size: 552 939 936 675 243 3301 Fragments arranged by size: 3301 939 936 675 552 243 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3172 487 Size: 683 56 1946 3961 Fragments arranged by size: 3961 1946 683 56 BtsI GCAGTG_nn' Cuts at: 2849 3630 4162 4956 4976 2849 Size: 781 532 794 20 4519 Fragments arranged by size: 4519 794 781 532 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6646 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6611 DraI TTT'AAA Cuts at: 3950 3950 Size: 6646 DraIII CAC_nnn'GTG Cuts at: 2571 3198 2571 Size: 627 6019 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5653 809 Size: 507 4337 1802 Fragments arranged by size: 4337 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2463 2604 2705 Size: 54 1425 50 275 18 633 141 101 Cuts at: 2705 2943 3073 3384 3838 3876 4093 4982 6611 Size: 238 130 311 454 38 217 889 1629 Cuts at: 6611 8 Size: 43 Fragments arranged by size: 1629 1425 889 633 454 311 275 238 217 141 130 101 54 50 43 38 18 EagI C'GGCC_G Cuts at: 1537 1812 4093 1537 Size: 275 2281 4090 Fragments arranged by size: 4090 2281 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2475 3233 4576 1353 Size: 1122 758 1343 3423 Fragments arranged by size: 3423 1343 1122 758 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2519 5233 5607 5753 387 Size: 1064 138 930 2714 374 146 1280 Fragments arranged by size: 2714 1280 1064 930 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2201 2318 2499 2682 2843 3109 3187 Size: 393 569 117 181 183 161 266 78 Cuts at: 3187 3601 3782 3833 4709 5306 6093 6361 1239 Size: 414 181 51 876 597 787 268 1524 Fragments arranged by size: 1524 876 787 597 569 414 393 268 266 183 181 181 161 117 78 51 EcoNI CCTnn'n_nnAGG Cuts at: 2787 3839 2787 Size: 1052 5594 EcoO109I rG'GnC_Cy Cuts at: 2009 2474 2616 2872 3118 3796 3832 2009 Size: 465 142 256 246 678 36 4823 Fragments arranged by size: 4823 678 465 256 246 142 36 EcoRI G'AATT_C Cuts at: 3293 3461 3955 4333 3293 Size: 168 494 378 5606 Fragments arranged by size: 5606 494 378 168 FspI TGC'GCA Cuts at: 5152 5152 Size: 6646 HaeII r_GCGC'y Cuts at: 1678 3088 3687 5793 1678 Size: 1410 599 2106 2531 Fragments arranged by size: 2531 2106 1410 599 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2062 2094 2372 2404 3314 Size: 32 135 32 490 32 278 32 910 Cuts at: 3314 3346 5287 5319 5361 5393 1373 Size: 32 1941 32 42 32 2626 Fragments arranged by size: 2626 1941 910 490 278 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3990 4236 669 Size: 988 281 2052 246 3079 Fragments arranged by size: 3079 2052 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6646 HpaI GTT'AAC Cuts at: 4236 4236 Size: 6646 KpnI G_GTAC'C Cuts at: 3971 3971 Size: 6646 MfeI C'AATT_G Cuts at: 4245 4245 Size: 6646 MluI A'CGCG_T Cuts at: 6522 6522 Size: 6646 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3942 5760 5944 1305 Size: 2637 1818 184 2007 Fragments arranged by size: 2637 2007 1818 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2465 2945 3075 3840 3878 Size: 54 1425 343 633 480 130 765 38 Cuts at: 3878 10 Size: 2778 Fragments arranged by size: 2778 1425 765 633 480 343 130 54 38 MslI CAynn'nnrTG Cuts at: 518 1166 2217 3141 3272 3872 4604 4963 5122 Size: 648 1051 924 131 600 732 359 159 Cuts at: 5122 518 Size: 2042 Fragments arranged by size: 2042 1051 924 732 648 600 359 159 131 MspA1I CmG'CkG Cuts at: 1606 2149 2229 2245 2887 3310 4062 4739 5887 Size: 543 80 16 642 423 752 677 1148 Cuts at: 5887 6132 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 752 677 642 543 423 245 80 16 NaeI GCC'GGC Cuts at: 2910 3148 3388 3783 2910 Size: 238 240 395 5773 Fragments arranged by size: 5773 395 240 238 NcoI C'CATG_G Cuts at: 513 1065 2004 3858 513 Size: 552 939 1854 3301 Fragments arranged by size: 3301 1854 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6646 NgoMIV G'CCGG_C Cuts at: 2908 3146 3386 3781 2908 Size: 238 240 395 5773 Fragments arranged by size: 5773 395 240 238 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6646 NotI GC'GGCC_GC Cuts at: 4093 4093 Size: 6646 NspI r_CATG'y Cuts at: 1132 1229 4004 5549 1132 Size: 97 2775 1545 2229 Fragments arranged by size: 2775 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2679 3828 1348 Size: 1331 1149 4166 Fragments arranged by size: 4166 1331 1149 PciI A'CATG_T Cuts at: 5545 5545 Size: 6646 PflMI CCAn_nnn'nTGG Cuts at: 1108 2660 2941 1108 Size: 1552 281 4813 Fragments arranged by size: 4813 1552 281 PmeI GTTT'AAAC Cuts at: 3950 3950 Size: 6646 PmlI CAC'GTG Cuts at: 2949 3139 2949 Size: 190 6456 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4715 4747 6228 6260 4715 Size: 32 1481 32 5101 Fragments arranged by size: 5101 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2474 2616 2872 2474 Size: 142 256 6248 Fragments arranged by size: 6248 256 142 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6646 PsiI TTA'TAA Cuts at: 4216 4216 Size: 6646 PspOMI G'GGCC_C Cuts at: 2008 2543 3831 2008 Size: 535 1288 4823 Fragments arranged by size: 4823 1288 535 PspXI vC'TCGA_Gb Cuts at: 2282 2282 Size: 6646 PstI C_TGCA'G Cuts at: 830 2154 2844 3109 3795 3998 830 Size: 1324 690 265 686 203 3478 Fragments arranged by size: 3478 1324 690 686 265 203 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6646 PvuII CAG'CTG Cuts at: 2149 2245 2887 3310 2149 Size: 96 642 423 5485 Fragments arranged by size: 5485 642 423 96 SalI G'TCGA_C Cuts at: 1936 3988 1936 Size: 2052 4594 SbfI CC_TGCA'GG Cuts at: 3795 3998 3795 Size: 203 6443 ScaI AGT'ACT Cuts at: 4894 6606 4894 Size: 1712 4934 SfcI C'TryA_G Cuts at: 826 1045 2150 2840 3105 3791 3994 5129 5810 Size: 219 1105 690 265 686 203 1135 681 Cuts at: 5810 6001 826 Size: 191 1471 Fragments arranged by size: 1471 1135 1105 690 686 681 265 219 203 191 SfiI GGCCn_nnn'nGGCC Cuts at: 3117 3117 Size: 6646 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6646 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6646 SmaI CCC'GGG Cuts at: 1872 3973 1872 Size: 2101 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2282 2876 4750 5651 5913 820 Size: 197 926 339 594 1874 901 262 1553 Fragments arranged by size: 1874 1553 926 901 594 339 262 197 SnaBI TAC'GTA Cuts at: 493 4437 493 Size: 3944 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6646 SphI G_CATG'C Cuts at: 1132 4004 1132 Size: 2872 3774 SspI AAT'ATT Cuts at: 5 52 4570 5 Size: 47 4518 2081 Fragments arranged by size: 4518 2081 47 StuI AGG'CCT Cuts at: 1517 3111 1517 Size: 1594 5052 StyI C'CwwG_G Cuts at: 513 1065 2004 3543 3835 3858 4085 513 Size: 552 939 1539 292 23 227 3074 Fragments arranged by size: 3074 1539 939 552 292 227 23 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2582 4673 4690 4843 5028 5473 325 Size: 1255 1002 2091 17 153 185 445 1498 Fragments arranged by size: 2091 1498 1255 1002 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3006 4892 6604 Size: 274 80 33 51 1443 1029 1886 1712 Cuts at: 6604 96 Size: 138 Fragments arranged by size: 1886 1712 1443 1029 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2458 2490 2951 2983 3436 3468 3486 3518 6606 Size: 32 461 32 453 32 18 32 3088 Cuts at: 6606 6638 2458 Size: 32 2466 Fragments arranged by size: 3088 2466 461 453 32 32 32 32 32 18 Tth111I GACn'n_nGTC Cuts at: 1316 2714 1316 Size: 1398 5248 XbaI T'CTAG_A Cuts at: 3982 3982 Size: 6646 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2242 2686 3622 2242 Size: 444 936 5266 Fragments arranged by size: 5266 936 444 XhoI C'TCGA_G Cuts at: 1943 2282 2876 1943 Size: 339 594 5713 Fragments arranged by size: 5713 594 339 XmaI C'CCGG_G Cuts at: 1870 3971 1870 Size: 2101 4545 XmnI GAAnn'nnTTC Cuts at: 4775 4775 Size: 6646 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2716 3748 4454 276 Size: 53 83 186 936 1182 1032 706 2468 Fragments arranged by size: 2468 1182 1032 936 706 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SalI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BstZ17I Bsu36I EcoICRI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PfoI PsrI RsrII SacI SacII SanDI SapI SexAI SfoI SrfI SwaI