Restriction map for
pFN21AE4180
(Linear) (Six-base) MAPSORT of: KIEE4180.seq Check: 3567 from: 1 to: 1941
KIEE4180.orf
With 182 enzymes: SgfI *
March 22, 2012 17:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 218 1941
Size: 218 1723
AatII G_ACGT'C
Cuts at: 0 713 1745 1941
Size: 713 1032 196
Fragments arranged by size:
1032 713 196
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 494 1182 1777 1828 1941
Size: 494 688 595 51 113
Fragments arranged by size:
688 595 494 113 51
AfeI AGC'GCT
Cuts at: 0 1081 1941
Size: 1081 860
AflIII A'CryG_T
Cuts at: 0 1727 1941
Size: 1727 214
AleI CACnn'nnGTG
Cuts at: 0 212 1136 1867 1941
Size: 212 924 731 74
Fragments arranged by size:
924 731 212 74
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1154 1941
Size: 1154 787
AlwNI CAG_nnn'CTG
Cuts at: 0 655 1682 1806 1941
Size: 655 1027 124 135
Fragments arranged by size:
1027 655 135 124
ApaI G_GGCC'C
Cuts at: 0 7 542 1830 1941
Size: 7 535 1288 111
Fragments arranged by size:
1288 535 111 7
ApoI r'AATT_y
Cuts at: 0 1288 1456 1941
Size: 1288 168 485
Fragments arranged by size:
1288 485 168
AvaI C'yCGr_G
Cuts at: 0 277 541 871 1941
Size: 277 264 330 1070
Fragments arranged by size:
1070 330 277 264
BanI G'GyrC_C
Cuts at: 0 1008 1269 1555 1941
Size: 1008 261 286 386
Fragments arranged by size:
1008 386 286 261
BanII G_rGCy'C
Cuts at: 0 7 542 824 1170 1477 1830 1941
Size: 7 535 282 346 307 353 111
Fragments arranged by size:
535 353 346 307 282 111 7
BbsI GAAGACnn'nnnn_
Cuts at: 0 1455 1941
Size: 1455 486
BbvCI CC'TCA_GC
Cuts at: 0 550 1941
Size: 550 1391
BglI GCCn_nnn'nGGC
Cuts at: 0 296 685 983 1112 1789 1941
Size: 296 389 298 129 677 152
Fragments arranged by size:
677 389 298 296 152 129
BlpI GC'TnA_GC
Cuts at: 0 1675 1941
Size: 1675 266
Bme1580I G_kGCm'C
Cuts at: 0 7 542 1274 1558 1830 1941
Size: 7 535 732 284 272 111
Fragments arranged by size:
732 535 284 272 111 7
BmgBI CAC'GTC
Cuts at: 0 1413 1586 1941
Size: 1413 173 355
Fragments arranged by size:
1413 355 173
BmrI ACTGGGnnnn_n'
Cuts at: 0 453 475 558 1941
Size: 453 22 83 1383
Fragments arranged by size:
1383 453 83 22
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 196 313 677 838 1104 1596 1941
Size: 196 117 364 161 266 492 345
Fragments arranged by size:
492 364 345 266 196 161 117
Bpu10I CC'TnA_GC
Cuts at: 0 550 1941
Size: 550 1391
BsaI GGTCTCn'nnnn_
Cuts at: 0 868 1207 1941
Size: 868 339 734
Fragments arranged by size:
868 734 339
BsaAI yAC'GTr
Cuts at: 0 944 1134 1941
Size: 944 190 807
Fragments arranged by size:
944 807 190
BsaHI Gr'CG_yC
Cuts at: 0 39 710 1367 1742 1941
Size: 39 671 657 375 199
Fragments arranged by size:
671 657 375 199 39
BsaWI w'CCGG_w
Cuts at: 0 68 1941
Size: 68 1873
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 946 976 1431 1461 1941
Size: 946 30 455 30 480
Fragments arranged by size:
946 480 455 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 493 604 928 1033 1452 1732 1941
Size: 493 111 324 105 419 280 209
Fragments arranged by size:
493 419 324 280 209 111 105
BseYI C'CCAG_C
Cuts at: 0 57 240 730 878 1406 1817 1941
Size: 57 183 490 148 528 411 124
Fragments arranged by size:
528 490 411 183 148 124 57
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 811 1114 1941
Size: 811 303 827
Fragments arranged by size:
827 811 303
BsiEI CG_ry'CG
Cuts at: 0 54 1941
Size: 54 1887
BsiHKAI G_wGCw'C
Cuts at: 0 110 995 1706 1941
Size: 110 885 711 235
Fragments arranged by size:
885 711 235 110
BsmBI CGTCTCn'nnnn_
Cuts at: 0 172 1941
Size: 172 1769
Bsp1286I G_dGCh'C
Cuts at: 0 7 110 542 824 995 1170 1274 1477
Size: 7 103 432 282 171 175 104 203
Cuts at: 1477 1558 1706 1830 1941
Size: 81 148 124 111
Fragments arranged by size:
432 282 203 175 171 148 124 111
104 103 81 7
BspEI T'CCGG_A
Cuts at: 0 68 1941
Size: 68 1873
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 218 1941
Size: 218 1723
BsrBI CCG'CTC
Cuts at: 0 1045 1547 1941
Size: 1045 502 394
Fragments arranged by size:
1045 502 394
BsrDI GCAATG_nn'
Cuts at: 0 1648 1941
Size: 1648 293
BsrFI r'CCGG_y
Cuts at: 0 53 903 1141 1381 1776 1941
Size: 53 850 238 240 395 165
Fragments arranged by size:
850 395 240 238 165 53
BssSI C'ACGA_G
Cuts at: 0 1210 1941
Size: 1210 731
BstAPI GCAn_nnn'nTGC
Cuts at: 0 834 1562 1941
Size: 834 728 379
Fragments arranged by size:
834 728 379
BstEII G'GTnAC_C
Cuts at: 0 1842 1941
Size: 1842 99
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1659 1854 1941
Size: 1659 195 87
Fragments arranged by size:
1659 195 87
BstYI r'GATC_y
Cuts at: 0 65 1802 1941
Size: 65 1737 139
Fragments arranged by size:
1737 139 65
BtgI C'CryG_G
Cuts at: 0 935 1610 1853 1941
Size: 935 675 243 88
Fragments arranged by size:
935 675 243 88
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1167 1941
Size: 1167 774
BtsI GCAGTG_nn'
Cuts at: 0 844 1625 1941
Size: 844 781 316
Fragments arranged by size:
844 781 316
DraIII CAC_nnn'GTG
Cuts at: 0 566 1193 1941
Size: 566 627 748
Fragments arranged by size:
748 627 566
EaeI y'GGCC_r
Cuts at: 0 458 599 700 938 1068 1379 1833 1871
Size: 458 141 101 238 130 311 454 38
Cuts at: 1871 1941
Size: 70
Fragments arranged by size:
458 454 311 238 141 130 101 70
38
EarI CTCTTCn'nnn_
Cuts at: 0 470 1228 1941
Size: 470 758 713
Fragments arranged by size:
758 713 470
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 514 1941
Size: 514 1427
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 196 313 494 677 838 1104 1182 1596
Size: 196 117 181 183 161 266 78 414
Cuts at: 1596 1777 1828 1941
Size: 181 51 113
Fragments arranged by size:
414 266 196 183 181 181 161 117
113 78 51
EcoNI CCTnn'n_nnAGG
Cuts at: 0 782 1834 1941
Size: 782 1052 107
Fragments arranged by size:
1052 782 107
EcoO109I rG'GnC_Cy
Cuts at: 0 4 469 611 867 1113 1791 1827 1941
Size: 4 465 142 256 246 678 36 114
Fragments arranged by size:
678 465 256 246 142 114 36 4
EcoRI G'AATT_C
Cuts at: 0 1288 1456 1941
Size: 1288 168 485
Fragments arranged by size:
1288 485 168
HaeII r_GCGC'y
Cuts at: 0 1083 1682 1941
Size: 1083 599 259
Fragments arranged by size:
1083 599 259
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 57 89 367 399 1309 1341 1941
Size: 57 32 278 32 910 32 600
Fragments arranged by size:
910 600 278 57 32 32 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1937 1941
Size: 1937 4
MscI TGG'CCA
Cuts at: 0 460 940 1070 1835 1873 1941
Size: 460 480 130 765 38 68
Fragments arranged by size:
765 480 460 130 68 38
MslI CAynn'nnrTG
Cuts at: 0 212 1136 1267 1867 1941
Size: 212 924 131 600 74
Fragments arranged by size:
924 600 212 131 74
MspA1I CmG'CkG
Cuts at: 0 144 224 240 882 1305 1941
Size: 144 80 16 642 423 636
Fragments arranged by size:
642 636 423 144 80 16
NaeI GCC'GGC
Cuts at: 0 905 1143 1383 1778 1941
Size: 905 238 240 395 163
Fragments arranged by size:
905 395 240 238 163
NcoI C'CATG_G
Cuts at: 0 1853 1941
Size: 1853 88
NgoMIV G'CCGG_C
Cuts at: 0 903 1141 1381 1776 1941
Size: 903 238 240 395 165
Fragments arranged by size:
903 395 240 238 165
PasI CC'CwG_GG
Cuts at: 0 674 1823 1941
Size: 674 1149 118
Fragments arranged by size:
1149 674 118
PflMI CCAn_nnn'nTGG
Cuts at: 0 655 936 1941
Size: 655 281 1005
Fragments arranged by size:
1005 655 281
PmlI CAC'GTG
Cuts at: 0 944 1134 1941
Size: 944 190 807
Fragments arranged by size:
944 807 190
PpuMI rG'GwC_Cy
Cuts at: 0 469 611 867 1941
Size: 469 142 256 1074
Fragments arranged by size:
1074 469 256 142
PspOMI G'GGCC_C
Cuts at: 0 3 538 1826 1941
Size: 3 535 1288 115
Fragments arranged by size:
1288 535 115 3
PspXI vC'TCGA_Gb
Cuts at: 0 277 1941
Size: 277 1664
PstI C_TGCA'G
Cuts at: 0 149 839 1104 1790 1941
Size: 149 690 265 686 151
Fragments arranged by size:
690 686 265 151 149
PvuII CAG'CTG
Cuts at: 0 144 240 882 1305 1941
Size: 144 96 642 423 636
Fragments arranged by size:
642 636 423 144 96
SbfI CC_TGCA'GG
Cuts at: 0 1790 1941
Size: 1790 151
SfcI C'TryA_G
Cuts at: 0 145 835 1100 1786 1941
Size: 145 690 265 686 155
Fragments arranged by size:
690 686 265 155 145
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1112 1941
Size: 1112 829
SmlI C'TyrA_G
Cuts at: 0 277 871 1941
Size: 277 594 1070
Fragments arranged by size:
1070 594 277
StuI AGG'CCT
Cuts at: 0 1106 1941
Size: 1106 835
StyI C'CwwG_G
Cuts at: 0 1538 1830 1853 1941
Size: 1538 292 23 88
Fragments arranged by size:
1538 292 88 23
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 577 1941
Size: 577 1364
TatI w'GTAC_w
Cuts at: 0 1001 1941
Size: 1001 940
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 453 485 946 978 1431 1463 1481 1513
Size: 453 32 461 32 453 32 18 32
Cuts at: 1513 1941
Size: 428
Fragments arranged by size:
461 453 453 428 32 32 32 32
18
Tth111I GACn'n_nGTC
Cuts at: 0 709 1941
Size: 709 1232
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 237 681 1617 1941
Size: 237 444 936 324
Fragments arranged by size:
936 444 324 237
XhoI C'TCGA_G
Cuts at: 0 277 871 1941
Size: 277 594 1070
Fragments arranged by size:
1070 594 277
ZraI GAC'GTC
Cuts at: 0 711 1743 1941
Size: 711 1032 198
Fragments arranged by size:
1032 711 198
Enzymes that do cut:
AarI AatII AcuI AfeI AflIII AleI AlfI AlwNI
ApaI ApoI AvaI BanI BanII BbsI BbvCI BglI
BlpI Bme1580I BmgBI BmrI BpmI Bpu10I BsaI BsaAI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmBI Bsp1286I BspEI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstEII BstXI BstYI BtgI BtgZI BtsI DraIII
EaeI EarI EciI Eco57MI EcoNI EcoO109I EcoRI HaeII
Hin4I MmeI MscI MslI MspA1I NaeI NcoI NgoMIV
PasI PflMI PmlI PpuMI PspOMI PspXI PstI PvuII
SbfI SfcI SfiI SmlI StuI StyI TaqII TatI
TstI Tth111I XcmI XhoI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AflII AgeI AhdI AloI ApaLI
AscI AseI AsiSI AvrII BaeI BamHI BbeI BcgI
BciVI BclI BfrBI BglII BmtI BplI BpuEI BsaBI
BsiWI BsmI BspHI BsrGI BssHII BstBI BstZ17I Bsu36I
ClaI CspCI DraI DrdI EagI EcoICRI EcoRV FalI
FseI FspI FspAI HincII HindIII HpaI KasI KpnI
MfeI MluI NarI NdeI NheI NotI NruI NsiI
NspI PacI PciI PfoI PmeI PpiI PshAI PsiI
PsrI PvuI RsrII SacI SacII SalI SanDI SapI
ScaI SexAI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI SwaI XbaI XmaI XmnI