################################## # NO CUT ENZYMES ( 159 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AcyI AflII AgeI AhdI AlwI AocI Aor51HI ApaI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BamHI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfaI BfrI BglII BlnI BpuAI BsaBI BsaHI BsaI BsaWI BseAI BsgI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrGI BsrI BssHII BssSI Bst1107I BstBI BstYI BstZ17I Bsu36I ClaI CpoI DpnII DraI DrdI Eam1105I EarI Eco47III Eco81I EcoNI EcoRI EcoRV EcoT14I EcoT22I FbaI FseI FspI HgaI Hin1I HincII HindII HindIII HinfI HpaI KasI KpnI Ksp632I KspI MaeI MamI MboI MboII MfeI MflI MluI MroI MseI MunI NarI NcoI NdeI NdeII NheI NlaIII NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmeI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI Sau3AI ScaI SexAI SfaNI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI SspBI SspI StuI StyI SwaI TfiI Tru9I Tsp509I Tth111I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 37 enzymes) # ################################### AccII 535 CGCG CG^CG AflIII 152 ACACGT A^C[GA][CT]GT Alw44I 334 GTGCAC G^TGCAC ApaLI 334 GTGCAC G^TGCAC AspHI 334 GTGCAC G[AT]GC[AT]^C BbrPI 256 CACGTG CAC^GTG BcnI 53 CCGGG CC^[GC]GG BlpI 31 GCTCAGC GC^T[ACGT]AGC Bpu1102I 31 GCTCAGC GC^T[ACGT]AGC BsaAI 256 CACGTG [CT]AC^GT[GA] BseRI 664 GAGGAG GAGGAG(10/8) BsiEI 50 CGGCCG CG[GA][CT]^CG BsiHKAI 334 GTGCAC G[AT]GC[AT]^C BsrDI 559 GCAATG GCAATG(2/0) BstAPI 603 GCAGCACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 312 GGTGACC G^GT[ACGT]ACC BstPI 312 GGTGACC G^GT[ACGT]ACC BstUI 535 CGCG CG^CG CelII 31 GCTCAGC GC^T[ACGT]AGC DraII 572 GGGACCC [GA]G^G[ACGT]CC[CT] DraIII 502 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG EagI 50 CGGCCG C^GGCCG EclXI 50 CGGCCG C^GGCCG Eco52I 50 CGGCCG C^GGCCG EcoO109I 572 GGGACCC [GA]G^G[ACGT]CC[CT] EcoO65I 312 GGTGACC G^GT[ACGT]ACC MaeIII 313 GTGAC ^GT[ACGT]AC MvnI 535 CGCG CG^CG NciI 53 CCGGG CC^[GC]GG PmaCI 256 CACGTG CAC^GTG PmlI 256 CACGTG CAC^GTG PpuMI 572 GGGACCC [GA]G^G[AT]CC[CT] PvuII 141 CAGCTG CAG^CTG SbfI 183 CCTGCAGG CCTGCA^GG Sse8387I 183 CCTGCAGG CCTGCA^GG Tsp45I 313 GTGAC ^GT[GC]AC TspRI 502 CACTG CA[GC]TG[ACGT][ACGT]^ ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### AfaI 90 GTAC GT^AC 459 GTAC AlwNI 487 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG 604 CAGCACCTG BalI 131 TGGCCA TGG^CCA 371 TGGCCA BsmAI 170 GTCTC GTCTC(1/5) 696 GTCTC BsmFI 572 GGGAC GGGAC(10/14) 391 GTCCC DdeI 32 CTCAG C^T[ACGT]AG 704 CTGAG DsaI 128 CCGTGG C^C[GA][CT]GG 395 CCGTGG HaeII 285 AGCGCC [GA]GCGC^[CT] 589 AGCGCC MaeII 154 ACGT A^CGT 257 ACGT MluNI 131 TGGCCA TGG^CCA 371 TGGCCA MscI 131 TGGCCA TGG^CCA 371 TGGCCA MslI 252 CACCCACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 639 CACCTTTGTG NaeI 48 GCCGGC GCC^GGC 57 GCCGGC NgoMIV 48 GCCGGC G^CCGGC 57 GCCGGC PstI 184 CTGCAG CTGCA^G 601 CTGCAG RsaI 90 GTAC GT^AC 459 GTAC TaqI 227 TCGA T^CGA 294 TCGA TthHB8I 227 TCGA T^CGA 294 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 44 enzymes) # ######################################### AciI AluI AvaII BanI BbvI BglI BmyI BpmI BsaJI BsiYI BslI Bsp1286I BspMI BsrFI BssKI BstF5I BstNI BstXI Cac8I CfoI Cfr10I Cfr13I EaeI EcoRII FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HpaII HphI ItaI MnlI MspA1I MspI MvaI MwoI NlaIV Sau96I ScrFI SfcI TseI