(Circular) (Six-base) MAPSORT of: pFN21AE4030.seq Check: 8953 from: 1 to: 5416 pFN21AE4030 5416 bp With 182 enzymes: SgfI * March 28, 2012 13:03 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2108 2182 2622 2108 Size: 74 440 4902 Fragments arranged by size: 4902 440 74 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3226 278 Size: 53 83 186 936 1690 2468 Fragments arranged by size: 2468 1690 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2759 1937 Size: 822 4594 Acc65I G'GTAC_C Cuts at: 2737 2737 Size: 5416 AclI AA'CG_TT Cuts at: 1563 2715 3543 3916 1563 Size: 1152 828 373 3063 Fragments arranged by size: 3063 1152 828 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2378 3479 4863 5131 1632 Size: 746 1101 1384 268 1917 Fragments arranged by size: 1917 1384 1101 746 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5219 AflIII A'CryG_T Cuts at: 1203 2157 4315 5292 1203 Size: 954 2158 977 1327 Fragments arranged by size: 2158 1327 977 954 AgeI A'CCGG_T Cuts at: 4247 4247 Size: 5416 AhdI GACnn_n'nnGTC Cuts at: 4145 4145 Size: 5416 AleI CACnn'nnGTG Cuts at: 1166 2261 2648 1166 Size: 1095 387 3934 Fragments arranged by size: 3934 1095 387 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5416 AlwNI CAG_nnn'CTG Cuts at: 2497 2614 3159 4731 2497 Size: 117 545 1572 3182 Fragments arranged by size: 3182 1572 545 117 ApaLI G'TGCA_C Cuts at: 2339 3473 4629 2339 Size: 1134 1156 3126 Fragments arranged by size: 3126 1156 1134 ApoI r'AATT_y Cuts at: 1493 2725 2951 3103 5212 1493 Size: 1232 226 152 2109 1697 Fragments arranged by size: 2109 1697 1232 226 152 AseI AT'TA_AT Cuts at: 160 1032 3970 160 Size: 872 2938 1606 Fragments arranged by size: 2938 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5416 AvaI C'yCGr_G Cuts at: 1870 1943 2741 1870 Size: 73 798 4545 Fragments arranged by size: 4545 798 73 BamHI G'GATC_C Cuts at: 2746 3113 2746 Size: 367 5049 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2194 2404 2471 2737 4192 Size: 325 217 627 407 210 67 266 1455 Cuts at: 4192 618 Size: 1842 Fragments arranged by size: 1842 1455 627 407 325 266 217 210 67 BanII G_rGCy'C Cuts at: 1391 2735 1391 Size: 1344 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4445 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3607 3641 1903 Size: 34 1670 34 3678 Fragments arranged by size: 3678 1670 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3308 4518 3308 Size: 1210 4206 BclI T'GATC_A Cuts at: 1548 1548 Size: 5416 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2059 2063 2684 Size: 107 122 71 631 605 387 4 621 Cuts at: 2684 4027 136 Size: 1343 1525 Fragments arranged by size: 1525 1343 631 621 605 387 122 107 71 4 BglII A'GATC_T Cuts at: 5411 5411 Size: 5416 BlpI GC'TnA_GC Cuts at: 2037 2833 2037 Size: 796 4620 Bme1580I G_kGCm'C Cuts at: 1790 2213 2343 2409 2476 2552 3477 4633 1790 Size: 423 130 66 67 76 925 1156 2573 Fragments arranged by size: 2573 1156 925 423 130 76 67 66 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5416 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3147 4105 442 Size: 416 53 83 399 24 1730 958 1753 Fragments arranged by size: 1753 1730 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5416 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2105 2135 2162 4076 1239 Size: 866 30 27 1914 2579 Fragments arranged by size: 2579 1914 866 30 27 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3541 4406 4704 3541 Size: 865 298 4253 Fragments arranged by size: 4253 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4079 882 Size: 617 2580 2219 Fragments arranged by size: 2580 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2263 3207 493 Size: 711 1059 944 2702 Fragments arranged by size: 2702 1059 944 711 BsaBI GATnn'nnATC Cuts at: 5410 5410 Size: 5416 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3223 3605 4240 275 Size: 53 83 186 936 1690 382 635 1451 Fragments arranged by size: 1690 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3849 4247 4521 4668 5079 3849 Size: 398 274 147 411 4186 Fragments arranged by size: 4186 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2078 2108 2618 2648 1178 Size: 30 165 30 675 30 510 30 3946 Fragments arranged by size: 3946 675 510 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2684 1186 Size: 1498 3918 BseYI C'CCAG_C Cuts at: 2426 2582 4619 5115 2426 Size: 156 2037 496 2727 Fragments arranged by size: 2727 2037 496 156 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4843 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2058 2866 3627 4655 1540 Size: 275 186 57 808 761 1028 2301 Fragments arranged by size: 2301 1028 808 761 275 186 57 BsiHKAI G_wGCw'C Cuts at: 2343 3477 3562 4633 2343 Size: 1134 85 1071 3126 Fragments arranged by size: 3126 1134 1071 85 BsmI GAATG_Cn' Cuts at: 2927 3020 2927 Size: 93 5323 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2213 2343 2409 2476 2552 2735 3477 Size: 399 423 130 66 67 76 183 742 Cuts at: 3477 3562 4633 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 423 399 183 130 85 76 67 66 BspEI T'CCGG_A Cuts at: 5079 5079 Size: 5416 BspHI T'CATG_A Cuts at: 3305 5035 3305 Size: 1730 3686 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2108 2182 2622 2771 844 Size: 1264 74 440 149 3489 Fragments arranged by size: 3489 1264 440 149 74 BsrBI CCG'CTC Cuts at: 1394 1823 3303 1394 Size: 429 1480 3507 Fragments arranged by size: 3507 1480 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2571 3911 4085 65 Size: 1183 1323 1340 174 1396 Fragments arranged by size: 1396 1340 1323 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2053 2062 2127 4060 4247 1700 Size: 110 243 9 65 1933 187 2869 Fragments arranged by size: 2869 1933 243 187 110 65 9 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5416 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5416 BssSI C'ACGA_G Cuts at: 3476 4488 3476 Size: 1012 4404 BstAPI GCAn_nnn'nTGC Cuts at: 794 2614 794 Size: 1820 3596 BstBI TT'CG_AA Cuts at: 4220 4220 Size: 5416 BstEII G'GTnAC_C Cuts at: 1184 2317 1184 Size: 1133 4283 BstXI CCAn_nnnn'nTGG Cuts at: 2113 2245 2263 2542 2113 Size: 132 18 279 4987 Fragments arranged by size: 4987 279 132 18 BstYI r'GATC_y Cuts at: 1972 2746 3113 3498 3515 4967 5053 5065 5411 Size: 774 367 385 17 1452 86 12 346 Cuts at: 5411 1972 Size: 1977 Fragments arranged by size: 1977 1452 774 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2133 2400 513 Size: 552 939 129 267 3529 Fragments arranged by size: 3529 939 552 267 129 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4677 Fragments arranged by size: 4677 683 56 BtsI GCAGTG_nn' Cuts at: 2932 3726 3746 2932 Size: 794 20 4602 Fragments arranged by size: 4602 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5416 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5381 DraI TTT'AAA Cuts at: 2720 2720 Size: 5416 DraIII CAC_nnn'GTG Cuts at: 2512 2512 Size: 5416 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4423 809 Size: 507 3107 1802 Fragments arranged by size: 3107 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2055 2136 2152 Size: 54 1425 50 275 18 225 81 16 Cuts at: 2152 2376 2863 3752 5381 8 Size: 224 487 889 1629 43 Fragments arranged by size: 1629 1425 889 487 275 225 224 81 54 50 43 18 16 EagI C'GGCC_G Cuts at: 1537 1812 2055 2863 1537 Size: 275 243 808 4090 Fragments arranged by size: 4090 808 275 243 EarI CTCTTCn'nnn_ Cuts at: 1353 3346 1353 Size: 1993 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4003 4377 4523 387 Size: 1064 138 2414 374 146 1280 Fragments arranged by size: 2414 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2105 2135 2162 2378 3479 4076 4863 Size: 393 473 30 27 216 1101 597 787 Cuts at: 4863 5131 1239 Size: 268 1524 Fragments arranged by size: 1524 1101 787 597 473 393 268 216 30 27 EcoO109I rG'GnC_Cy Cuts at: 2578 2578 Size: 5416 EcoRI G'AATT_C Cuts at: 2725 3103 2725 Size: 378 5038 FspI TGC'GCA Cuts at: 3922 3922 Size: 5416 HaeII r_GCGC'y Cuts at: 1678 2294 2598 4563 1678 Size: 616 304 1965 2531 Fragments arranged by size: 2531 1965 616 304 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4057 4089 4131 4163 1373 Size: 32 135 32 2485 32 42 32 2626 Fragments arranged by size: 2626 2485 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2760 3006 669 Size: 988 281 822 246 3079 Fragments arranged by size: 3079 988 822 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5416 HpaI GTT'AAC Cuts at: 3006 3006 Size: 5416 KpnI G_GTAC'C Cuts at: 2741 2741 Size: 5416 MfeI C'AATT_G Cuts at: 3015 3015 Size: 5416 MluI A'CGCG_T Cuts at: 5292 5292 Size: 5416 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4530 4714 1305 Size: 3225 184 2007 Fragments arranged by size: 3225 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2138 2378 10 Size: 54 1425 343 306 240 3048 Fragments arranged by size: 3048 1425 343 306 240 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2261 2648 3374 3733 3892 518 Size: 648 843 252 387 726 359 159 2042 Fragments arranged by size: 2042 843 726 648 387 359 252 159 MspA1I CmG'CkG Cuts at: 1606 2044 2079 2148 2206 2832 3509 4657 4902 Size: 438 35 69 58 626 677 1148 245 Cuts at: 4902 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 626 438 245 69 58 35 NaeI GCC'GGC Cuts at: 2055 2064 2055 Size: 9 5407 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3925 Fragments arranged by size: 3925 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5416 NgoMIV G'CCGG_C Cuts at: 2053 2062 2053 Size: 9 5407 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5416 NotI GC'GGCC_GC Cuts at: 2863 2863 Size: 5416 NspI r_CATG'y Cuts at: 1132 1229 2774 4319 1132 Size: 97 1545 1545 2229 Fragments arranged by size: 2229 1545 1545 97 PasI CC'CwG_GG Cuts at: 1348 2571 1348 Size: 1223 4193 PciI A'CATG_T Cuts at: 4315 4315 Size: 5416 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5416 PfoI T'CCnGG_A Cuts at: 2364 2364 Size: 5416 PmeI GTTT'AAAC Cuts at: 2720 2720 Size: 5416 PmlI CAC'GTG Cuts at: 2263 2263 Size: 5416 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3485 3517 4998 5030 3485 Size: 32 1481 32 3871 Fragments arranged by size: 3871 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2578 2578 Size: 5416 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5416 PsiI TTA'TAA Cuts at: 2986 2986 Size: 5416 PstI C_TGCA'G Cuts at: 830 2193 2610 2768 830 Size: 1363 417 158 3478 Fragments arranged by size: 3478 1363 417 158 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5416 PvuII CAG'CTG Cuts at: 2148 2148 Size: 5416 SalI G'TCGA_C Cuts at: 1936 2758 1936 Size: 822 4594 SanDI GG'GwC_CC Cuts at: 2578 2578 Size: 5416 SbfI CC_TGCA'GG Cuts at: 2193 2768 2193 Size: 575 4841 ScaI AGT'ACT Cuts at: 3664 5376 3664 Size: 1712 3704 SfcI C'TryA_G Cuts at: 826 1045 2189 2606 2656 2764 3899 4580 4771 Size: 219 1144 417 50 108 1135 681 191 Cuts at: 4771 826 Size: 1471 Fragments arranged by size: 1471 1144 1135 681 417 219 191 108 50 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5416 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5416 SmaI CCC'GGG Cuts at: 1872 2743 1872 Size: 871 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3520 4421 4683 820 Size: 197 926 1577 901 262 1553 Fragments arranged by size: 1577 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3207 493 Size: 2714 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5416 SphI G_CATG'C Cuts at: 1132 2774 1132 Size: 1642 3774 SspI AAT'ATT Cuts at: 5 52 3340 5 Size: 47 3288 2081 Fragments arranged by size: 3288 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5416 StyI C'CwwG_G Cuts at: 513 1065 2004 2855 513 Size: 552 939 851 3074 Fragments arranged by size: 3074 939 851 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2273 3443 3460 3613 3798 4243 325 Size: 1255 693 1170 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1170 693 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2094 3662 5374 Size: 274 80 33 51 1443 117 1568 1712 Cuts at: 5374 96 Size: 138 Fragments arranged by size: 1712 1568 1443 274 138 117 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2333 2365 2406 2438 2618 2650 5376 5408 2333 Size: 32 41 32 180 32 2726 32 2341 Fragments arranged by size: 2726 2341 180 41 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5416 XbaI T'CTAG_A Cuts at: 2752 2752 Size: 5416 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5416 XmaI C'CCGG_G Cuts at: 1870 2741 1870 Size: 871 4545 XmnI GAAnn'nnTTC Cuts at: 3545 3545 Size: 5416 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3224 276 Size: 53 83 186 936 1690 2468 Fragments arranged by size: 2468 1690 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PspOMI PspXI PsrI RsrII SacI SacII SapI SexAI SfiI SfoI SrfI SwaI XcmI