(Linear) (Six-base) MAPSORT of: KIEE4030.seq Check: 9147 from: 1 to: 711 KIEE4030.orf With 182 enzymes: SgfI * March 22, 2012 17:12 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 103 177 617 711 Size: 103 74 440 94 Fragments arranged by size: 440 103 94 74 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 373 711 Size: 373 338 AflIII A'CryG_T Cuts at: 0 152 711 Size: 152 559 AleI CACnn'nnGTG Cuts at: 0 256 643 711 Size: 256 387 68 Fragments arranged by size: 387 256 68 AlwNI CAG_nnn'CTG Cuts at: 0 492 609 711 Size: 492 117 102 Fragments arranged by size: 492 117 102 ApaLI G'TGCA_C Cuts at: 0 334 711 Size: 334 377 BanI G'GyrC_C Cuts at: 0 189 399 466 711 Size: 189 210 67 245 Fragments arranged by size: 245 210 189 67 BglI GCCn_nnn'nGGC Cuts at: 0 54 58 679 711 Size: 54 4 621 32 Fragments arranged by size: 621 54 32 4 BlpI GC'TnA_GC Cuts at: 0 32 711 Size: 32 679 Bme1580I G_kGCm'C Cuts at: 0 208 338 404 471 547 711 Size: 208 130 66 67 76 164 Fragments arranged by size: 208 164 130 76 67 66 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 100 130 157 711 Size: 100 30 27 554 Fragments arranged by size: 554 100 30 27 BsaAI yAC'GTr Cuts at: 0 258 711 Size: 258 453 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 73 103 613 643 711 Size: 73 30 510 30 68 Fragments arranged by size: 510 73 68 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 679 711 Size: 679 32 BseYI C'CCAG_C Cuts at: 0 421 577 711 Size: 421 156 134 Fragments arranged by size: 421 156 134 BsiEI CG_ry'CG Cuts at: 0 53 711 Size: 53 658 BsiHKAI G_wGCw'C Cuts at: 0 338 711 Size: 338 373 Bsp1286I G_dGCh'C Cuts at: 0 208 338 404 471 547 711 Size: 208 130 66 67 76 164 Fragments arranged by size: 208 164 130 76 67 66 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 103 177 617 711 Size: 103 74 440 94 Fragments arranged by size: 440 103 94 74 BsrDI GCAATG_nn' Cuts at: 0 566 711 Size: 566 145 BsrFI r'CCGG_y Cuts at: 0 48 57 122 711 Size: 48 9 65 589 Fragments arranged by size: 589 65 48 9 BstAPI GCAn_nnn'nTGC Cuts at: 0 609 711 Size: 609 102 BstEII G'GTnAC_C Cuts at: 0 312 711 Size: 312 399 BstXI CCAn_nnnn'nTGG Cuts at: 0 108 240 258 537 711 Size: 108 132 18 279 174 Fragments arranged by size: 279 174 132 108 18 BtgI C'CryG_G Cuts at: 0 128 395 711 Size: 128 267 316 Fragments arranged by size: 316 267 128 DraIII CAC_nnn'GTG Cuts at: 0 507 711 Size: 507 204 EaeI y'GGCC_r Cuts at: 0 50 131 147 371 711 Size: 50 81 16 224 340 Fragments arranged by size: 340 224 81 50 16 EagI C'GGCC_G Cuts at: 0 50 711 Size: 50 661 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 100 130 157 373 711 Size: 100 30 27 216 338 Fragments arranged by size: 338 216 100 30 27 EcoO109I rG'GnC_Cy Cuts at: 0 573 711 Size: 573 138 HaeII r_GCGC'y Cuts at: 0 289 593 711 Size: 289 304 118 Fragments arranged by size: 304 289 118 MscI TGG'CCA Cuts at: 0 133 373 711 Size: 133 240 338 Fragments arranged by size: 338 240 133 MslI CAynn'nnrTG Cuts at: 0 256 643 711 Size: 256 387 68 Fragments arranged by size: 387 256 68 MspA1I CmG'CkG Cuts at: 0 39 74 143 201 711 Size: 39 35 69 58 510 Fragments arranged by size: 510 69 58 39 35 NaeI GCC'GGC Cuts at: 0 50 59 711 Size: 50 9 652 Fragments arranged by size: 652 50 9 NgoMIV G'CCGG_C Cuts at: 0 48 57 711 Size: 48 9 654 Fragments arranged by size: 654 48 9 PasI CC'CwG_GG Cuts at: 0 566 711 Size: 566 145 PfoI T'CCnGG_A Cuts at: 0 359 711 Size: 359 352 PmlI CAC'GTG Cuts at: 0 258 711 Size: 258 453 PpuMI rG'GwC_Cy Cuts at: 0 573 711 Size: 573 138 PstI C_TGCA'G Cuts at: 0 188 605 711 Size: 188 417 106 Fragments arranged by size: 417 188 106 PvuII CAG'CTG Cuts at: 0 143 711 Size: 143 568 SanDI GG'GwC_CC Cuts at: 0 573 711 Size: 573 138 SbfI CC_TGCA'GG Cuts at: 0 188 711 Size: 188 523 SfcI C'TryA_G Cuts at: 0 184 601 651 711 Size: 184 417 50 60 Fragments arranged by size: 417 184 60 50 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 268 711 Size: 268 443 TatI w'GTAC_w Cuts at: 0 89 711 Size: 89 622 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 328 360 401 433 613 645 711 Size: 328 32 41 32 180 32 66 Fragments arranged by size: 328 180 66 41 32 32 32 Enzymes that do cut: AarI AcuI AflIII AleI AlwNI ApaLI BanI BglI BlpI Bme1580I BpmI BsaAI BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspMI BsrDI BsrFI BstAPI BstEII BstXI BtgI DraIII EaeI EagI Eco57MI EcoO109I HaeII MscI MslI MspA1I NaeI NgoMIV PasI PfoI PmlI PpuMI PstI PvuII SanDI SbfI SfcI TaqII TatI TstI Enzymes that do not cut: AatII AccI Acc65I AclI AfeI AflII AgeI AhdI AlfI AloI ApaI ApoI AscI AseI AsiSI AvaI AvrII BaeI BamHI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglII BmgBI BmrI BmtI BplI Bpu10I BpuEI BsaI BsaBI BsaHI BsaWI BsgI BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrGI BssHII BssSI BstBI BstYI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI DrdI EarI EciI EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI FspAI Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI NarI NcoI NdeI NheI NotI NruI NsiI NspI PacI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacI SacII SalI SapI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI SwaI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI