Restriction map for
pFN21AE4016
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# NO CUT ENZYMES ( 170 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AfaI AflII AflIII
AgeI AhdI Alw44I AlwNI AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AviII AvrII BalI BamHI BanI
BbeI BbrPI BbsI BbvCI BciVI
BfaI BfrI BglI BlnI BlpI
BpmI Bpu1102I BpuAI BsaAI BsaBI
BsaHI BseAI BsiEI BsiWI BsmBI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrDI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstXI BstZ17I
Bsu36I Cac8I CelII CfoI ClaI
CpoI DraI DraII DraIII DrdI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRI EcoRV EcoT14I EcoT22I FseI
FspI HaeII HgaI HhaI Hin1I
HinP1I HincII HindII HindIII HpaI
KasI KpnI KspI MaeI MamI
MfeI MluI MluNI MroI MscI
MslI MunI MvnI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI RcaI RsaI RsrII
SacII SalI SbfI ScaI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI StyI SwaI Tsp45I Tth111I
Van91I XbaI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AcsI 523 GAATTT [GA]^AATT[CT]
AlwI 194 GGATC GGATC(4/5)
ApoI 523 GAATTT [GA]^AATT[CT]
AspHI 37 GAGCTC G[AT]GC[AT]^C
BanII 37 GAGCTC G[GA]GC[CT]^C
BcgI 419 GCATGATGGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 489 TGATCA T^GATCA
BcnI 141 CCGGG CC^[GC]GG
BglII 386 AGATCT A^GATCT
BmyI 37 GAGCTC G[AGT]GC[ACT]^C
BsaI 58 GAGACC GGTCTC(1/5)
BsaJI 240 CCGTGG C^C[ACGT][ACGT]GG
BsaWI 191 ACCGGA [AT]^CCGG[AT]
BseRI 43 GAGGAG GAGGAG(10/8)
BsgI 343 CTGCAC GTGCAG(16/14)
BsiHKAI 37 GAGCTC G[AT]GC[AT]^C
BsmAI 58 GAGAC GTCTC(1/5)
Bsp1286I 37 GAGCTC G[AGT]GC[ACT]^C
BsrI 183 ACTGG ACTGG(1/-1)
BstYI 386 AGATCT [GA]^GATC[CT]
DsaI 240 CCGTGG C^C[GA][CT]GG
EarI 34 GAAGAG CTCTTC(1/4)
FauI 27 GCGGG CCCGC(4/6)
FbaI 489 TGATCA T^GATCA
HaeIII 12 GGCC GG^CC
HphI 104 TCACC GGTGA(8/7)
Ksp632I 34 GAAGAG CTCTTC(1/4)
MaeII 19 ACGT A^CGT
MflI 386 AGATCT [GA]^GATC[CT]
MseI 530 TTAA T^TAA
MspA1I 451 CAGCTG C[AC]G^C[GT]G
MwoI 89 GCCTCTACAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NciI 141 CCGGG CC^[GC]GG
PaeR7I 40 CTCGAG C^TCGAG
PleI 132 GACTC GAGTC(4/5)
PvuII 451 CAGCTG CAG^CTG
SacI 37 GAGCTC GAGCT^C
SapI 34 GAAGAGC GCTCTTC(1/4)
SexAI 227 ACCAGGT A^CC[AT]GGT
SfcI 93 CTACAG C^T[GA][CT]AG
SmlI 40 CTCGAG C^T[CT][GA]AG
Tru9I 530 TTAA T^TAA
Tsp509I 524 AATT ^AATT
XcmI 173 CCATCAACCCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 40 CTCGAG C^TCGAG
XhoII 386 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 23 enzymes) #
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AciI 270 CCGC CCGC(-3/-1)
27 GCGG
AvaI 8 CTCGGG C^[CT]CG[GA]G
40 CTCGAG
BbvI 450 GCAGC GCAGC(8/12)
458 GCAGC
BsiYI 180 CCCACTGGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
273 CCCCATTGAGG
BslI 180 CCCACTGGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
273 CCCCATTGAGG
BsoBI 8 CTCGGG C^[CT]CG[GA]G
40 CTCGAG
BsrFI 61 ACCGGC [GA]^CCGG[CT]
98 GCCGGT
BstNI 111 CCTGG CC^[AT]GG
228 CCAGG
Cfr10I 61 ACCGGC [GA]^CCGG[CT]
98 GCCGGT
DdeI 136 CTCAG C^T[ACGT]AG
441 CTCAG
EcoRII 111 CCTGG ^CC[AT]GG
228 CCAGG
Fnu4HI 450 GCAGC GC^[ACGT]GC
458 GCAGC
ItaI 450 GCAGC GC^[ACGT]GC
458 GCAGC
MaeIII 21 GTTAC ^GT[ACGT]AC
405 GTTAC
MvaI 111 CCTGG CC^[AT]GG
228 CCAGG
NlaIII 249 CATG CATG^
420 CATG
NlaIV 188 GGGACC GG[ACGT]^[ACGT]CC
310 GGACCC
SfaNI 461 GCATC GCATC(5/9)
569 GCATC
TaqI 41 TCGA T^CGA
357 TCGA
TfiI 159 GATTC G^A[AT]TC
245 GATTC
TseI 450 GCAGC G^C[AT]GC
458 GCAGC
TspRI 182 CACTG CA[GC]TG[ACGT][ACGT]^
504 CAGTG
TthHB8I 41 TCGA T^CGA
357 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 19 enzymes) #
#########################################
AluI AvaII BsmFI BssKI BstF5I
Cfr13I DpnII FokI HapII HinfI
HpaII MboI MboII MnlI MspI
NdeII Sau3AI Sau96I ScrFI