################################## # NO CUT ENZYMES ( 178 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwNI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AviII AvrII BalI BanI BanII BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BfrI BglI BglII BlnI BmyI BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsmFI BsmI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I Cfr10I ClaI CpoI DraI DraIII DrdI DsaI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I EcoNI EcoO65I EcoRI EcoRV EcoT14I EcoT22I FauI FbaI FseI FspI Hin1I HincII HindII HindIII HpaI HphI KasI KpnI Ksp632I KspI MaeII MaeIII MamI MfeI MluI MluNI MnlI MroI MscI MslI MunI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfaNI SfiI SfoI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspBI StuI StyI SwaI TaqI Tsp45I TspRI Tth111I TthHB8I Van91I XbaI XcmI XhoI XmnI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### AfaI 111 GTAC GT^AC AocI 114 CCTAAGG CC^T[ACGT]AGG AvaI 37 CCCGGG C^[CT]CG[GA]G AvaII 119 GGACC G^G[AT]CC BamHI 219 GGATCC G^GATCC BfaI 193 CTAG C^TAG BlpI 45 GCTTAGC GC^T[ACGT]AGC BpmI 61 CTGGAG CTGGAG(16/14) Bpu1102I 45 GCTTAGC GC^T[ACGT]AGC BsmAI 4 GAGAC GTCTC(1/5) BsmBI 4 GAGACG CGTCTC(1/5) BsoBI 37 CCCGGG C^[CT]CG[GA]G BstF5I 189 CATCC GGATG(2/0) BstNI 13 CCAGG CC^[AT]GG BstYI 219 GGATCC [GA]^GATC[CT] Bsu36I 114 CCTAAGG CC^T[ACGT]AGG Cac8I 17 GCTTGC GC[ACGT]^[ACGT]GC CelII 45 GCTTAGC GC^T[ACGT]AGC DpnII 220 GATC ^GATC DraII 118 AGGACCC [GA]G^G[ACGT]CC[CT] Eco81I 114 CCTAAGG CC^T[ACGT]AGG EcoO109I 118 AGGACCC [GA]G^G[ACGT]CC[CT] EcoRII 13 CCAGG ^CC[AT]GG FokI 189 CATCC GGATG(9/13) HaeII 42 GGCGCT [GA]GCGC^[CT] HaeIII 29 GGCC GG^CC HapII 38 CCGG C^CGG HgaI 6 GACGC GACGC(5/10) HpaII 38 CCGG C^CGG MaeI 193 CTAG C^TAG MboI 220 GATC ^GATC MboII 155 TCTTC GAAGA(8/7) MflI 219 GGATCC [GA]^GATC[CT] MseI 257 TTAA T^TAA MspI 38 CCGG C^CGG MvaI 13 CCAGG CC^[AT]GG NdeII 220 GATC ^GATC NlaIII 246 CATG CATG^ NspI 245 ACATGC [GA]CATG^[CT] PleI 90 GACTC GAGTC(4/5) PpuMI 118 AGGACCC [GA]G^G[AT]CC[CT] RsaI 111 GTAC GT^AC Sau3AI 220 GATC ^GATC SmaI 37 CCCGGG CCC^GGG SspI 165 AATATT AAT^ATT Tru9I 257 TTAA T^TAA XhoII 219 GGATCC [GA]^GATC[CT] XmaCI 37 CCCGGG C^CCGGG XmaI 37 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 16 enzymes) # ################################### AciI 27 GCGG CCGC(-3/-1) 271 GCGG AcsI 76 AAATTC [GA]^AATT[CT] 196 GAATTT AlwI 220 GATCC GGATC(4/5) 219 GGATC ApoI 76 AAATTC [GA]^AATT[CT] 196 GAATTT BcnI 37 CCCGG CC^[GC]GG 38 CCGGG BsiYI 31 CCCGACCCCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 32 CCGACCCCGGG BslI 31 CCCGACCCCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 32 CCGACCCCGGG Cfr13I 29 GGCCC G^G[ACGT]CC 119 GGACC DdeI 46 CTTAG C^T[ACGT]AG 115 CTAAG NciI 37 CCCGG CC^[GC]GG 38 CCGGG NlaIV 119 GGACCC GG[ACGT]^[ACGT]CC 219 GGATCC PstI 22 CTGCAG CTGCA^G 203 CTGCAG PvuII 58 CAGCTG CAG^CTG 67 CAGCTG Sau96I 29 GGCCC G^G[ACGT]CC 119 GGACC SfcI 22 CTGCAG C^T[GA][CT]AG 203 CTGCAG TfiI 84 GATTC G^A[AT]TC 240 GATTC ######################################### # 3 OR MORE CUT ENZYMES ( 15 enzymes) # ######################################### AluI BbvI BsaJI BssKI CfoI Fnu4HI HhaI HinP1I HinfI ItaI MspA1I MwoI ScrFI TseI Tsp509I