################################## # NO CUT ENZYMES ( 136 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AfaI AflII AflIII AgeI AhdI Alw44I AlwNI AocI ApaLI AseI AsnI Asp718I AspEI AspHI AviII AvrII BalI BbrPI BbsI BbvCI BcgI BciVI BclI BfrI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaWI BseAI BsiHKAI BsiWI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrGI BsrI BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I Bsu36I CelII ClaI CpoI DraI DraIII DrdI Eam1105I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI KpnI MaeII MaeIII MamI MfeI MluI MluNI MroI MscI MseI MslI MunI NcoI NdeI NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflMI PinAI PleI PmaCI PmeI PmlI PshBI Psp1406I PstI PvuI RcaI RsaI RsrII SacI SalI SapI SbfI ScaI SexAI SfcI SfiI SfuI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tru9I Tsp45I Van91I XbaI XhoI ################################### # ONE CUT ENZYMES ( 31 enzymes) # ################################### AcsI 88 AAATTC [GA]^AATT[CT] Aor51HI 102 AGCGCT AGC^GCT ApaI 222 GGGCCC GGGCC^C ApoI 88 AAATTC [GA]^AATT[CT] AscI 700 GGCGCGCC GG^CGCGCC Asp700I 537 GAACAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspI 187 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC BfaI 340 CTAG C^TAG BsaI 205 GAGACC GGTCTC(1/5) BsgI 781 CTGCAC GTGCAG(16/14) BsiEI 362 CGGCCG CG[GA][CT]^CG BsmBI 610 GAGACG CGTCTC(1/5) BsrBI 313 GAGCGG CCGCTC(-3/-3) DdeI 598 CTCAG C^T[ACGT]AG EagI 362 CGGCCG C^GGCCG EclXI 362 CGGCCG C^GGCCG Eco47III 102 AGCGCT AGC^GCT Eco52I 362 CGGCCG C^GGCCG HinfI 636 GAATC G^A[ACGT]TC MaeI 340 CTAG C^TAG NlaIII 8 CATG CATG^ PflFI 187 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC PshAI 612 GACGCAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC TaqI 158 TCGA T^CGA TfiI 636 GAATC G^A[AT]TC Tsp509I 89 AATT ^AATT TspRI 566 CAGTG CA[GC]TG[ACGT][ACGT]^ Tth111I 187 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC TthHB8I 158 TCGA T^CGA XcmI 137 CCAGGAAAAGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmnI 537 GAACAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### BamHI 288 GGATCC G^GATCC 430 GGATCC BspMI 615 GCAGGT ACCTGC(4/8) 687 GCAGGT BstF5I 72 CATCC GGATG(2/0) 279 GGATG BstYI 288 GGATCC [GA]^GATC[CT] 430 GGATCC DpnII 289 GATC ^GATC 431 GATC DraII 185 GGGACCC [GA]G^G[ACGT]CC[CT] 418 AGGTCCC EarI 823 CTCTTC CTCTTC(1/4) 268 GAAGAG EcoO109I 185 GGGACCC [GA]G^G[ACGT]CC[CT] 418 AGGTCCC FokI 72 CATCC GGATG(9/13) 279 GGATG Ksp632I 823 CTCTTC CTCTTC(1/4) 268 GAAGAG MboI 289 GATC ^GATC 431 GATC MflI 288 GGATCC [GA]^GATC[CT] 430 GGATCC NaeI 232 GCCGGC GCC^GGC 458 GCCGGC NdeII 289 GATC ^GATC 431 GATC NgoMIV 232 GCCGGC G^CCGGC 458 GCCGGC PpuMI 185 GGGACCC [GA]G^G[AT]CC[CT] 418 AGGTCCC PvuII 199 CAGCTG CAG^CTG 520 CAGCTG Sau3AI 289 GATC ^GATC 431 GATC SgrAI 457 CGCCGGCG C[GA]^CCGG[CT]G 771 CACCGGCG XhoII 288 GGATCC [GA]^GATC[CT] 430 GGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 71 enzymes) # ######################################### AccII AciI AcyI AluI AlwI AvaI AvaII BanI BanII BbeI BbvI BcnI BglI BmyI BpmI BsaHI BsaJI BseRI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrFI BssHII BssKI BstNI BstUI Cac8I CfoI Cfr10I Cfr13I DsaI EaeI EcoRII EcoT14I FauI Fnu4HI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HpaII HphI ItaI KasI KspI MboII MnlI MspA1I MspI MvaI MvnI MwoI NarI NciI NlaIV SacII Sau96I ScrFI SfaNI SfoI SmaI StyI TseI XmaCI XmaI