################################## # NO CUT ENZYMES ( 102 enzymes) # ################################## AatII Acc65I AccI AccIII AcsI AcyI AflII AgeI AocI Aor51HI ApoI AscI AseI AsnI Asp718I AspI BamHI BbeI BbrPI BbvCI BcgI BfrI BsaAI BsaHI BsaWI BseAI BsiEI BsiWI BsmBI Bsp1407I BspDI BspEI BspHI BsrGI BssHII BssSI Bst1107I BstEII BstPI BstZ17I Bsu36I ClaI CpoI DraI DrdI EagI EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV FseI Hin1I HincII HindII HpaI KasI KpnI Ksp632I MfeI MluI MroI MunI NarI NotI PacI PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI PvuI RcaI RsrII SalI SapI SbfI SexAI SfaNI SfiI SfoI SgrAI SmaI SnaBI SpeI Sse8387I SspBI SspI StuI SwaI Tth111I Van91I XbaI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 60 enzymes) # ################################### AclI 804 AACGTT AA^CGTT AflIII 1481 ACATGT A^C[GA][CT]GT AhdI 632 GACAGGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 1795 GTGCAC G^TGCAC AlwI 460 GATCC GGATC(4/5) ApaI 150 GGGCCC GGGCC^C ApaLI 1795 GTGCAC G^TGCAC AspEI 632 GACAGGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvaI 725 CTCGAG C^[CT]CG[GA]G AviII 977 TGCGCA TGC^GCA AvrII 1576 CCTAGG C^CTAGG BalI 1398 TGGCCA TGG^CCA BanI 1279 GGCACC G^G[CT][GA]CC BciVI 1555 GGATAC GTATCC(6/5) BglI 143 GCCCCGCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 16 AGATCT A^GATCT BlnI 1576 CCTAGG C^CTAGG BlpI 138 GCTGAGC GC^T[ACGT]AGC Bpu1102I 138 GCTGAGC GC^T[ACGT]AGC BsaBI 460 GATCCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 966 GAGACC GGTCTC(1/5) BsgI 700 CTGCAC GTGCAG(16/14) BsmFI 1063 GGGAC GGGAC(10/14) BsmI 958 GCATTC GAATGC(1/-1) BsoBI 725 CTCGAG C^[CT]CG[GA]G BspLU11I 1481 ACATGT A^CATGT BspMI 1670 ACCTGC ACCTGC(4/8) BsrBI 247 GAGCGG CCGCTC(-3/-3) BsrFI 1751 GCCGGC [GA]^CCGG[CT] BstBI 991 TTCGAA TT^CGAA CelII 138 GCTGAGC GC^T[ACGT]AGC Cfr10I 1751 GCCGGC [GA]^CCGG[CT] DraIII 1199 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG Eam1105I 632 GACAGGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoT22I 956 ATGCAT ATGCA^T FspI 977 TGCGCA TGC^GCA HgaI 172 GACGC GACGC(5/10) HindIII 658 AAGCTT A^AGCTT KspI 146 CCGCGG CCGC^GG MaeII 805 ACGT A^CGT MamI 460 GATCCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluNI 1398 TGGCCA TGG^CCA MscI 1398 TGGCCA TGG^CCA MseI 871 TTAA T^TAA NaeI 1751 GCCGGC GCC^GGC NcoI 326 CCATGG C^CATGG NdeI 953 CATATG CA^TATG NgoMIV 1751 GCCGGC G^CCGGC NheI 1581 GCTAGC G^CTAGC NruI 37 TCGCGA TCG^CGA NsiI 956 ATGCAT ATGCA^T NspV 991 TTCGAA TT^CGAA PaeR7I 725 CTCGAG C^TCGAG PleI 1326 GACTC GAGTC(4/5) Psp1406I 804 AACGTT AA^CGTT SacII 146 CCGCGG CCGC^GG SfuI 991 TTCGAA TT^CGAA SphI 1694 GCATGC GCATG^C Tru9I 871 TTAA T^TAA XhoI 725 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### Asp700I 369 GAAGCGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 801 GAAAACGTTC BbsI 994 GAAGAC GAAGAC(2/6) 321 GTCTTC BclI 398 TGATCA T^GATCA 681 TGATCA BcnI 201 CCCGG CC^[GC]GG 1593 CCCGG BpuAI 994 GAAGAC GAAGAC(2/6) 321 GTCTTC BstXI 378 CCATGATATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 539 CCAAGTGGATGG BstYI 16 AGATCT [GA]^GATC[CT] 459 AGATCC DsaI 146 CCGCGG C^C[GA][CT]GG 326 CCATGG EaeI 1398 TGGCCA [CT]^GGCC[GA] 1749 TGGCCG EcoT14I 326 CCATGG C^C[AT][AT]GG 1576 CCTAGG FauI 145 CCCGC CCCGC(4/6) 148 GCGGG FbaI 398 TGATCA T^GATCA 681 TGATCA HaeII 135 GGCGCT [GA]GCGC^[CT] 1606 AGCGCC MflI 16 AGATCT [GA]^GATC[CT] 459 AGATCC NciI 201 CCCGG CC^[GC]GG 1593 CCCGG PpuMI 236 AGGTCCT [GA]G^G[AT]CC[CT] 1408 GGGTCCT PstI 20 CTGCAG CTGCA^G 296 CTGCAG PvuII 1168 CAGCTG CAG^CTG 1402 CAGCTG SacI 33 GAGCTC GAGCT^C 1509 GAGCTC ScaI 268 AGTACT AGT^ACT 473 AGTACT SfcI 20 CTGCAG C^T[GA][CT]AG 296 CTGCAG SmlI 655 CTCAAG C^T[CT][GA]AG 725 CTCGAG StyI 326 CCATGG C^C[AT][AT]GG 1576 CCTAGG TaqI 726 TCGA T^CGA 992 TCGA Tsp45I 758 GTGAC ^GT[GC]AC 1250 GTGAC Tsp509I 876 AATT ^AATT 1561 AATT TthHB8I 726 TCGA T^CGA 992 TCGA XhoII 16 AGATCT [GA]^GATC[CT] 459 AGATCC XmnI 369 GAAGCGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 801 GAAAACGTTC ######################################### # 3 OR MORE CUT ENZYMES ( 67 enzymes) # ######################################### AccII AciI AfaI AluI AlwNI AspHI AvaII BanII BbvI BfaI BmyI BpmI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI Bsp1286I BsrDI BsrI BssKI BstAPI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DdeI DpnII DraII EcoO109I EcoRII Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MaeI MaeIII MboI MboII MnlI MslI MspA1I MspI MvaI MvnI MwoI NdeII NlaIII NlaIV NspI RsaI Sau3AI Sau96I ScrFI TfiI TseI TspRI XcmI