(Circular) (Six-base) MAPSORT of: pFN21AE3427.seq Check: 7947 from: 1 to: 6511 pFN21AE3427 6511 bp With 182 enzymes: SgfI * March 28, 2012 12:54 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4321 278 Size: 53 83 186 936 2785 2468 Fragments arranged by size: 2785 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3854 1937 Size: 1917 4594 Acc65I G'GTAC_C Cuts at: 3832 3832 Size: 6511 AclI AA'CG_TT Cuts at: 1563 2810 4638 5011 1563 Size: 1247 1828 373 3063 Fragments arranged by size: 3063 1828 1247 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2254 2740 3260 3276 4574 5958 6226 1632 Size: 622 486 520 16 1298 1384 268 1917 Fragments arranged by size: 1917 1384 1298 622 520 486 268 16 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6314 AflIII A'CryG_T Cuts at: 1203 3486 5410 6387 1203 Size: 2283 1924 977 1327 Fragments arranged by size: 2283 1924 1327 977 AgeI A'CCGG_T Cuts at: 5342 5342 Size: 6511 AhdI GACnn_n'nnGTC Cuts at: 2642 5240 2642 Size: 2598 3913 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6511 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3521 1184 Size: 2337 4174 AlwNI CAG_nnn'CTG Cuts at: 2237 2309 3676 4254 5826 2237 Size: 72 1367 578 1572 2922 Fragments arranged by size: 2922 1572 1367 578 72 ApaI G_GGCC'C Cuts at: 2159 2159 Size: 6511 ApaLI G'TGCA_C Cuts at: 3800 4568 5724 3800 Size: 768 1156 4587 Fragments arranged by size: 4587 1156 768 ApoI r'AATT_y Cuts at: 1493 3820 4046 4198 6307 1493 Size: 2327 226 152 2109 1697 Fragments arranged by size: 2327 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5065 160 Size: 872 4033 1606 Fragments arranged by size: 4033 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6511 AvaI C'yCGr_G Cuts at: 1870 1943 2730 3836 1870 Size: 73 787 1106 4545 Fragments arranged by size: 4545 1106 787 73 AvrII C'CTAG_G Cuts at: 3581 3581 Size: 6511 BamHI G'GATC_C Cuts at: 3841 4208 3841 Size: 367 6144 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3284 3832 5287 618 Size: 325 217 627 1497 548 1455 1842 Fragments arranged by size: 1842 1497 1455 627 548 325 217 BanII G_rGCy'C Cuts at: 1391 2042 2150 2159 3518 3830 1391 Size: 651 108 9 1359 312 4072 Fragments arranged by size: 4072 1359 651 312 108 9 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2319 3006 928 Size: 971 420 687 4433 Fragments arranged by size: 4433 971 687 420 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4702 4736 1903 Size: 34 2765 34 3678 Fragments arranged by size: 3678 2765 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3554 4403 5613 3554 Size: 849 1210 4452 Fragments arranged by size: 4452 1210 849 BclI T'GATC_A Cuts at: 1548 2403 2686 1548 Size: 855 283 5373 Fragments arranged by size: 5373 855 283 BfrBI ATG'CAT Cuts at: 2963 2963 Size: 6511 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2154 5122 136 Size: 107 122 71 631 605 482 2968 1525 Fragments arranged by size: 2968 1525 631 605 482 122 107 71 BglII A'GATC_T Cuts at: 2021 6506 2021 Size: 4485 2026 BlpI GC'TnA_GC Cuts at: 2144 3928 2144 Size: 1784 4727 Bme1580I G_kGCm'C Cuts at: 1790 2159 2583 3214 3804 4572 5728 1790 Size: 369 424 631 590 768 1156 2573 Fragments arranged by size: 2573 1156 768 631 590 424 369 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6511 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2116 3075 3424 Size: 416 53 83 399 24 699 959 349 Cuts at: 3424 4242 5200 442 Size: 818 958 1753 Fragments arranged by size: 1753 959 958 818 699 416 399 349 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3590 1056 Size: 2534 3977 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3633 3633 Size: 6511 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2216 2640 2989 3043 3451 5171 1239 Size: 977 424 349 54 408 1720 2579 Fragments arranged by size: 2579 1720 977 424 408 349 54 Bpu10I CC'TnA_GC Cuts at: 2036 2036 Size: 6511 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2645 4636 5501 5799 2645 Size: 1991 865 298 3357 Fragments arranged by size: 3357 1991 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2965 5174 882 Size: 617 1466 2209 2219 Fragments arranged by size: 2219 2209 1466 617 BsaAI yAC'GTr Cuts at: 493 1204 4302 493 Size: 711 3098 2702 Fragments arranged by size: 3098 2702 711 BsaBI GATnn'nnATC Cuts at: 2469 6505 2469 Size: 4036 2475 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4318 4700 5335 275 Size: 53 83 186 936 2785 382 635 1451 Fragments arranged by size: 2785 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4944 5342 5616 5763 6174 4944 Size: 398 274 147 411 5281 Fragments arranged by size: 5281 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6286 Fragments arranged by size: 6286 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2126 2351 3108 3351 3463 3772 3775 1186 Size: 940 225 757 243 112 309 3 3922 Fragments arranged by size: 3922 940 757 309 243 225 112 3 BseYI C'CCAG_C Cuts at: 3409 5714 6210 3409 Size: 2305 496 3710 Fragments arranged by size: 3710 2305 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2690 1163 Size: 573 954 4984 Fragments arranged by size: 4984 954 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3961 4722 5750 1540 Size: 275 186 1960 761 1028 2301 Fragments arranged by size: 2301 1960 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2042 2050 2225 3518 3696 3804 4572 4657 5728 Size: 8 175 1293 178 108 768 85 1071 Cuts at: 5728 2042 Size: 2825 Fragments arranged by size: 2825 1293 1071 768 178 175 108 85 8 BsmI GAATG_Cn' Cuts at: 2963 4022 4115 2963 Size: 1059 93 5359 Fragments arranged by size: 5359 1059 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2042 2050 2150 2159 2225 2583 3214 Size: 399 252 8 100 9 66 358 631 Cuts at: 3214 3518 3696 3804 3830 4572 4657 5728 1391 Size: 304 178 108 26 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 631 399 358 304 252 178 108 100 85 66 26 9 8 BspEI T'CCGG_A Cuts at: 6174 6174 Size: 6511 BspHI T'CATG_A Cuts at: 4400 6130 4400 Size: 1730 4781 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3684 3866 844 Size: 2840 182 3489 Fragments arranged by size: 3489 2840 182 BsrBI CCG'CTC Cuts at: 1394 1823 2254 4398 1394 Size: 429 431 2144 3507 Fragments arranged by size: 3507 2144 431 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2363 2496 2569 3463 5006 5180 65 Size: 1183 1115 133 73 894 1543 174 1396 Fragments arranged by size: 1543 1396 1183 1115 894 174 133 73 BsrFI r'CCGG_y Cuts at: 1700 1810 3756 5155 5342 1700 Size: 110 1946 1399 187 2869 Fragments arranged by size: 2869 1946 1399 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6511 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6511 BssSI C'ACGA_G Cuts at: 4571 5583 4571 Size: 1012 5499 BstAPI GCAn_nnn'nTGC Cuts at: 794 2713 3458 3676 3700 794 Size: 1919 745 218 24 3605 Fragments arranged by size: 3605 1919 745 218 24 BstBI TT'CG_AA Cuts at: 2997 5315 2997 Size: 2318 4193 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6511 BstXI CCAn_nnnn'nTGG Cuts at: 2390 2551 2390 Size: 161 6350 BstYI r'GATC_y Cuts at: 1972 2021 2464 3841 4208 4593 4610 6062 6148 Size: 49 443 1377 367 385 17 1452 86 Cuts at: 6148 6160 6506 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1377 443 385 367 346 86 49 17 12 BtgI C'CryG_G Cuts at: 513 1065 2151 2331 513 Size: 552 1086 180 4693 Fragments arranged by size: 4693 1086 552 180 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5772 Fragments arranged by size: 5772 683 56 BtsI GCAGTG_nn' Cuts at: 2767 4027 4821 4841 2767 Size: 1260 794 20 4437 Fragments arranged by size: 4437 1260 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6511 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6476 DraI TTT'AAA Cuts at: 3815 3815 Size: 6511 DraIII CAC_nnn'GTG Cuts at: 3209 3209 Size: 6511 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5518 809 Size: 507 4202 1802 Fragments arranged by size: 4202 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3403 3754 3958 Size: 54 1425 50 275 18 1573 351 204 Cuts at: 3958 4847 6476 8 Size: 889 1629 43 Fragments arranged by size: 1629 1573 1425 889 351 275 204 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3958 1537 Size: 275 2146 4090 Fragments arranged by size: 4090 2146 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4441 1353 Size: 3088 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5098 5472 5618 387 Size: 1064 138 3509 374 146 1280 Fragments arranged by size: 3509 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2040 3516 2040 Size: 1476 5035 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2216 2254 2640 2740 2989 3043 3260 Size: 393 584 38 386 100 249 54 217 Cuts at: 3260 3276 3451 4574 5171 5958 6226 1239 Size: 16 175 1123 597 787 268 1524 Fragments arranged by size: 1524 1123 787 597 584 393 386 268 249 217 175 100 54 38 16 EcoO109I rG'GnC_Cy Cuts at: 2242 2306 3414 3654 2242 Size: 64 1108 240 5099 Fragments arranged by size: 5099 1108 240 64 EcoRI G'AATT_C Cuts at: 3820 4198 3820 Size: 378 6133 FspI TGC'GCA Cuts at: 2984 5017 2984 Size: 2033 4478 FspAI rTGC'GCAy Cuts at: 2984 2984 Size: 6511 HaeII r_GCGC'y Cuts at: 1678 2144 3615 5658 1678 Size: 466 1471 2043 2531 Fragments arranged by size: 2531 2043 1471 466 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2013 2045 2276 2308 2436 Size: 32 135 32 441 32 231 32 128 Cuts at: 2436 2468 2628 2660 5152 5184 5226 5258 1373 Size: 32 160 32 2492 32 42 32 2626 Fragments arranged by size: 2626 2492 441 231 160 135 128 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3855 4101 669 Size: 988 281 1917 246 3079 Fragments arranged by size: 3079 1917 988 281 246 HindIII A'AGCT_T Cuts at: 748 2663 748 Size: 1915 4596 HpaI GTT'AAC Cuts at: 4101 4101 Size: 6511 KpnI G_GTAC'C Cuts at: 3836 3836 Size: 6511 MfeI C'AATT_G Cuts at: 4110 4110 Size: 6511 MluI A'CGCG_T Cuts at: 6387 6387 Size: 6511 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2661 3146 3672 5625 5809 1305 Size: 1356 485 526 1953 184 2007 Fragments arranged by size: 2007 1953 1356 526 485 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3405 10 Size: 54 1425 343 1573 3116 Fragments arranged by size: 3116 1573 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2444 2489 2630 3011 3704 4469 4828 Size: 648 1278 45 141 381 693 765 359 Cuts at: 4828 4987 518 Size: 159 2042 Fragments arranged by size: 2042 1278 765 693 648 381 359 159 141 45 MspA1I CmG'CkG Cuts at: 1606 2153 3175 3409 3927 4604 5752 5997 1606 Size: 547 1022 234 518 677 1148 245 2120 Fragments arranged by size: 2120 1148 1022 677 547 518 245 234 NaeI GCC'GGC Cuts at: 3758 3758 Size: 6511 NcoI C'CATG_G Cuts at: 513 1065 2331 513 Size: 552 1266 4693 Fragments arranged by size: 4693 1266 552 NdeI CA'TA_TG Cuts at: 387 2959 387 Size: 2572 3939 NgoMIV G'CCGG_C Cuts at: 3756 3756 Size: 6511 NheI G'CTAG_C Cuts at: 1052 3586 1052 Size: 2534 3977 NotI GC'GGCC_GC Cuts at: 3958 3958 Size: 6511 NruI TCG'CGA Cuts at: 2044 2044 Size: 6511 NsiI A_TGCA'T Cuts at: 2965 2965 Size: 6511 NspI r_CATG'y Cuts at: 1132 1229 2054 3490 3508 3703 3869 5414 1132 Size: 97 825 1436 18 195 166 1545 2229 Fragments arranged by size: 2229 1545 1436 825 195 166 97 18 PasI CC'CwG_GG Cuts at: 1348 3245 1348 Size: 1897 4614 PciI A'CATG_T Cuts at: 3486 5410 3486 Size: 1924 4587 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6511 PmeI GTTT'AAAC Cuts at: 3815 3815 Size: 6511 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2212 2244 2816 2848 4580 4612 6093 6125 2212 Size: 32 572 32 1732 32 1481 32 2598 Fragments arranged by size: 2598 1732 1481 572 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2242 3414 2242 Size: 1172 5339 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6511 PsiI TTA'TAA Cuts at: 4081 4081 Size: 6511 PspOMI G'GGCC_C Cuts at: 2155 2155 Size: 6511 PspXI vC'TCGA_Gb Cuts at: 2730 2730 Size: 6511 PstI C_TGCA'G Cuts at: 830 2029 2305 3863 830 Size: 1199 276 1558 3478 Fragments arranged by size: 3478 1558 1199 276 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6511 PvuII CAG'CTG Cuts at: 3175 3409 3175 Size: 234 6277 SacI G_AGCT'C Cuts at: 2042 3518 2042 Size: 1476 5035 SacII CC_GC'GG Cuts at: 2154 2154 Size: 6511 SalI G'TCGA_C Cuts at: 1936 3853 1936 Size: 1917 4594 SbfI CC_TGCA'GG Cuts at: 3863 3863 Size: 6511 ScaI AGT'ACT Cuts at: 2275 2480 4759 6471 2275 Size: 205 2279 1712 2315 Fragments arranged by size: 2315 2279 1712 205 SfcI C'TryA_G Cuts at: 826 1045 2025 2301 3859 4994 5675 5866 826 Size: 219 980 276 1558 1135 681 191 1471 Fragments arranged by size: 1558 1471 1135 980 681 276 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6511 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6511 SmaI CCC'GGG Cuts at: 1872 3838 1872 Size: 1966 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2660 2730 4615 5516 5778 820 Size: 197 926 717 70 1885 901 262 1553 Fragments arranged by size: 1885 1553 926 901 717 262 197 70 SnaBI TAC'GTA Cuts at: 493 4302 493 Size: 3809 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6511 SphI G_CATG'C Cuts at: 1132 3703 3869 1132 Size: 2571 166 3774 Fragments arranged by size: 3774 2571 166 SspI AAT'ATT Cuts at: 5 52 4435 5 Size: 47 4383 2081 Fragments arranged by size: 4383 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6511 StyI C'CwwG_G Cuts at: 513 1065 2331 3581 3950 513 Size: 552 1266 1250 369 3074 Fragments arranged by size: 3074 1266 1250 552 369 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2788 2901 3736 4538 4555 4708 4893 Size: 1255 1208 113 835 802 17 153 185 Cuts at: 4893 5338 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1208 835 802 445 185 153 113 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2273 2478 4757 Size: 274 80 33 51 1443 296 205 2279 Cuts at: 4757 6469 96 Size: 1712 138 Fragments arranged by size: 2279 1712 1443 296 274 205 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6471 6503 6471 Size: 32 6479 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6511 XbaI T'CTAG_A Cuts at: 3847 3847 Size: 6511 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2329 2551 2677 2329 Size: 222 126 6163 Fragments arranged by size: 6163 222 126 XhoI C'TCGA_G Cuts at: 1943 2730 1943 Size: 787 5724 XmaI C'CCGG_G Cuts at: 1870 3836 1870 Size: 1966 4545 XmnI GAAnn'nnTTC Cuts at: 2378 2810 4640 2378 Size: 432 1830 4249 Fragments arranged by size: 4249 1830 432 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4319 276 Size: 53 83 186 936 2785 2468 Fragments arranged by size: 2785 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI FspAI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI BaeI BbeI BbvCI BsiWI BsmBI BstZ17I Bsu36I EcoNI EcoRV FalI FseI KasI NarI PacI PfoI PmlI PsrI RsrII SanDI SapI SexAI SfiI SfoI SrfI SwaI