(Linear) (Six-base) MAPSORT of: KIEE3427.seq Check: 1399 from: 1 to: 1806 KIEE3427.orf With 182 enzymes: SgfI * March 22, 2012 16:33 .. AclI AA'CG_TT Cuts at: 0 805 1806 Size: 805 1001 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 249 735 1255 1271 1806 Size: 249 486 520 16 535 Fragments arranged by size: 535 520 486 249 16 AflIII A'CryG_T Cuts at: 0 1481 1806 Size: 1481 325 AhdI GACnn_n'nnGTC Cuts at: 0 637 1806 Size: 637 1169 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1516 1806 Size: 1516 290 AlwNI CAG_nnn'CTG Cuts at: 0 232 304 1671 1806 Size: 232 72 1367 135 Fragments arranged by size: 1367 232 135 72 ApaI G_GGCC'C Cuts at: 0 154 1806 Size: 154 1652 ApaLI G'TGCA_C Cuts at: 0 1795 1806 Size: 1795 11 AvaI C'yCGr_G Cuts at: 0 725 1806 Size: 725 1081 AvrII C'CTAG_G Cuts at: 0 1576 1806 Size: 1576 230 BanI G'GyrC_C Cuts at: 0 1279 1806 Size: 1279 527 BanII G_rGCy'C Cuts at: 0 37 145 154 1513 1806 Size: 37 108 9 1359 293 Fragments arranged by size: 1359 293 108 37 9 BbsI GAAGACnn'nnnn_ Cuts at: 0 314 1001 1806 Size: 314 687 805 Fragments arranged by size: 805 687 314 BciVI GTATCCnnnnn_n' Cuts at: 0 1549 1806 Size: 1549 257 BclI T'GATC_A Cuts at: 0 398 681 1806 Size: 398 283 1125 Fragments arranged by size: 1125 398 283 BfrBI ATG'CAT Cuts at: 0 958 1806 Size: 958 848 BglI GCCn_nnn'nGGC Cuts at: 0 149 1806 Size: 149 1657 BglII A'GATC_T Cuts at: 0 16 1806 Size: 16 1790 BlpI GC'TnA_GC Cuts at: 0 139 1806 Size: 139 1667 Bme1580I G_kGCm'C Cuts at: 0 154 578 1209 1799 1806 Size: 154 424 631 590 7 Fragments arranged by size: 631 590 424 154 7 BmrI ACTGGGnnnn_n' Cuts at: 0 111 1070 1419 1806 Size: 111 959 349 387 Fragments arranged by size: 959 387 349 111 BmtI G_CTAG'C Cuts at: 0 1585 1806 Size: 1585 221 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1628 1806 Size: 1628 178 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 211 635 984 1038 1446 1806 Size: 211 424 349 54 408 360 Fragments arranged by size: 424 408 360 349 211 54 Bpu10I CC'TnA_GC Cuts at: 0 31 1806 Size: 31 1775 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 640 1806 Size: 640 1166 BsaI GGTCTCn'nnnn_ Cuts at: 0 960 1806 Size: 960 846 BsaBI GATnn'nnATC Cuts at: 0 464 1806 Size: 464 1342 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 121 346 1103 1346 1458 1767 1770 1806 Size: 121 225 757 243 112 309 3 36 Fragments arranged by size: 757 309 243 225 121 112 36 3 BseYI C'CCAG_C Cuts at: 0 1404 1806 Size: 1404 402 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 685 1806 Size: 685 1121 BsiHKAI G_wGCw'C Cuts at: 0 37 45 220 1513 1691 1799 1806 Size: 37 8 175 1293 178 108 7 Fragments arranged by size: 1293 178 175 108 37 8 7 BsmI GAATG_Cn' Cuts at: 0 958 1806 Size: 958 848 Bsp1286I G_dGCh'C Cuts at: 0 37 45 145 154 220 578 1209 1513 Size: 37 8 100 9 66 358 631 304 Cuts at: 1513 1691 1799 1806 Size: 178 108 7 Fragments arranged by size: 631 358 304 178 108 100 66 37 9 8 7 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1679 1806 Size: 1679 127 BsrBI CCG'CTC Cuts at: 0 249 1806 Size: 249 1557 BsrDI GCAATG_nn' Cuts at: 0 358 491 564 1458 1806 Size: 358 133 73 894 348 Fragments arranged by size: 894 358 348 133 73 BsrFI r'CCGG_y Cuts at: 0 1751 1806 Size: 1751 55 BstAPI GCAn_nnn'nTGC Cuts at: 0 708 1453 1671 1695 1806 Size: 708 745 218 24 111 Fragments arranged by size: 745 708 218 111 24 BstBI TT'CG_AA Cuts at: 0 992 1806 Size: 992 814 BstXI CCAn_nnnn'nTGG Cuts at: 0 385 546 1806 Size: 385 161 1260 Fragments arranged by size: 1260 385 161 BstYI r'GATC_y Cuts at: 0 16 459 1806 Size: 16 443 1347 Fragments arranged by size: 1347 443 16 BtgI C'CryG_G Cuts at: 0 146 326 1806 Size: 146 180 1480 Fragments arranged by size: 1480 180 146 BtsI GCAGTG_nn' Cuts at: 0 762 1806 Size: 762 1044 DraIII CAC_nnn'GTG Cuts at: 0 1204 1806 Size: 1204 602 EaeI y'GGCC_r Cuts at: 0 1398 1749 1806 Size: 1398 351 57 Fragments arranged by size: 1398 351 57 EcoICRI GAG'CTC Cuts at: 0 35 1511 1806 Size: 35 1476 295 Fragments arranged by size: 1476 295 35 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 211 249 635 735 984 1038 1255 1271 Size: 211 38 386 100 249 54 217 16 Cuts at: 1271 1446 1806 Size: 175 360 Fragments arranged by size: 386 360 249 217 211 175 100 54 38 16 EcoO109I rG'GnC_Cy Cuts at: 0 237 301 1409 1649 1806 Size: 237 64 1108 240 157 Fragments arranged by size: 1108 240 237 157 64 FspI TGC'GCA Cuts at: 0 979 1806 Size: 979 827 FspAI rTGC'GCAy Cuts at: 0 979 1806 Size: 979 827 HaeII r_GCGC'y Cuts at: 0 139 1610 1806 Size: 139 1471 196 Fragments arranged by size: 1471 196 139 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 8 40 271 303 431 463 623 655 Size: 8 32 231 32 128 32 160 32 Cuts at: 655 1806 Size: 1151 Fragments arranged by size: 1151 231 160 128 32 32 32 32 8 HindIII A'AGCT_T Cuts at: 0 658 1806 Size: 658 1148 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 656 1141 1667 1806 Size: 656 485 526 139 Fragments arranged by size: 656 526 485 139 MscI TGG'CCA Cuts at: 0 1400 1806 Size: 1400 406 MslI CAynn'nnrTG Cuts at: 0 439 484 625 1006 1699 1806 Size: 439 45 141 381 693 107 Fragments arranged by size: 693 439 381 141 107 45 MspA1I CmG'CkG Cuts at: 0 148 1170 1404 1806 Size: 148 1022 234 402 Fragments arranged by size: 1022 402 234 148 NaeI GCC'GGC Cuts at: 0 1753 1806 Size: 1753 53 NcoI C'CATG_G Cuts at: 0 326 1806 Size: 326 1480 NdeI CA'TA_TG Cuts at: 0 954 1806 Size: 954 852 NgoMIV G'CCGG_C Cuts at: 0 1751 1806 Size: 1751 55 NheI G'CTAG_C Cuts at: 0 1581 1806 Size: 1581 225 NruI TCG'CGA Cuts at: 0 39 1806 Size: 39 1767 NsiI A_TGCA'T Cuts at: 0 960 1806 Size: 960 846 NspI r_CATG'y Cuts at: 0 49 1485 1503 1698 1806 Size: 49 1436 18 195 108 Fragments arranged by size: 1436 195 108 49 18 PasI CC'CwG_GG Cuts at: 0 1240 1806 Size: 1240 566 PciI A'CATG_T Cuts at: 0 1481 1806 Size: 1481 325 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 207 239 811 843 1806 Size: 207 32 572 32 963 Fragments arranged by size: 963 572 207 32 32 PpuMI rG'GwC_Cy Cuts at: 0 237 1409 1806 Size: 237 1172 397 Fragments arranged by size: 1172 397 237 PspOMI G'GGCC_C Cuts at: 0 150 1806 Size: 150 1656 PspXI vC'TCGA_Gb Cuts at: 0 725 1806 Size: 725 1081 PstI C_TGCA'G Cuts at: 0 24 300 1806 Size: 24 276 1506 Fragments arranged by size: 1506 276 24 PvuII CAG'CTG Cuts at: 0 1170 1404 1806 Size: 1170 234 402 Fragments arranged by size: 1170 402 234 SacI G_AGCT'C Cuts at: 0 37 1513 1806 Size: 37 1476 293 Fragments arranged by size: 1476 293 37 SacII CC_GC'GG Cuts at: 0 149 1806 Size: 149 1657 ScaI AGT'ACT Cuts at: 0 270 475 1806 Size: 270 205 1331 Fragments arranged by size: 1331 270 205 SfcI C'TryA_G Cuts at: 0 20 296 1806 Size: 20 276 1510 Fragments arranged by size: 1510 276 20 SmlI C'TyrA_G Cuts at: 0 655 725 1806 Size: 655 70 1081 Fragments arranged by size: 1081 655 70 SphI G_CATG'C Cuts at: 0 1698 1806 Size: 1698 108 StyI C'CwwG_G Cuts at: 0 326 1576 1806 Size: 326 1250 230 Fragments arranged by size: 1250 326 230 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 783 896 1731 1806 Size: 783 113 835 75 Fragments arranged by size: 835 783 113 75 TatI w'GTAC_w Cuts at: 0 268 473 1806 Size: 268 205 1333 Fragments arranged by size: 1333 268 205 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 324 546 672 1806 Size: 324 222 126 1134 Fragments arranged by size: 1134 324 222 126 XhoI C'TCGA_G Cuts at: 0 725 1806 Size: 725 1081 XmnI GAAnn'nnTTC Cuts at: 0 373 805 1806 Size: 373 432 1001 Fragments arranged by size: 1001 432 373 Enzymes that do cut: AclI AcuI AflIII AhdI AlfI AlwNI ApaI ApaLI AvaI AvrII BanI BanII BbsI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaBI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspMI BsrBI BsrDI BsrFI BstAPI BstBI BstXI BstYI BtgI BtsI DraIII EaeI EcoICRI Eco57MI EcoO109I FspI FspAI HaeII Hin4I HindIII MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NruI NsiI NspI PasI PciI PpiI PpuMI PspOMI PspXI PstI PvuII SacI SacII ScaI SfcI SmlI SphI StyI TaqII TatI XcmI XhoI XmnI Enzymes that do not cut: AarI AatII AccI Acc65I AfeI AflII AgeI AleI AloI ApoI AscI AseI AsiSI BaeI BamHI BbeI BbvCI BcgI BmgBI BsaAI BsaHI BsaWI BsaXI BsiEI BsiWI BsmBI BspEI BspHI BsrGI BssHII BssSI BstEII BstZ17I Bsu36I BtgZI ClaI CspCI DraI DrdI EagI EarI EciI EcoNI EcoRI EcoRV FalI FseI HincII HpaI KasI KpnI MfeI MluI NarI NotI PacI PflMI PfoI PmeI PmlI PshAI PsiI PsrI PvuI RsrII SalI SanDI SapI SbfI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI SspI StuI SwaI TstI Tth111I XbaI XmaI ZraI