################################## # NO CUT ENZYMES ( 111 enzymes) # ################################## AatII AclI AcyI AflII AgeI AhdI Alw44I Aor51HI ApaI ApaLI AscI AseI AsnI AspEI AspI AviII AvrII BamHI BbeI BbrPI BbsI BciVI BclI BfaI BfrI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaHI BsiWI BsmI Bsp1407I BspDI BspLU11I BsrBI BsrGI BssSI Bst1107I BstAPI BstBI BstXI BstZ17I CelII ClaI CpoI DraI DraIII EagI Eam1105I EclXI Eco47III Eco52I EcoRV EcoT22I FbaI FseI FspI HgaI Hin1I HincII HindII HindIII HpaI KasI KspI MaeI MfeI MluI MslI MunI NarI NdeI NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RsrII SacII SalI ScaI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI SspBI SspI StuI SwaI Tth111I XbaI XcmI XhoI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### Acc65I 827 GGTACC G^GTACC AccIII 992 TCCGGA T^CCGGA AflIII 1595 ACGTGT A^C[GA][CT]GT AocI 513 CCTGAGG CC^T[ACGT]AGG Asp700I 1510 GAATTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 827 GGTACC G^GTACC BbvCI 159 CCTCAGC CCTCAGC(-5/-2) BcgI 740 GCAGGTTCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BglI 454 GCCACCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaBI 798 GATGACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BseAI 992 TCCGGA T^CCGGA BsgI 922 CTGCAC GTGCAG(16/14) BsiEI 632 CGACCG CG[GA][CT]^CG BsmBI 1570 GAGACG CGTCTC(1/5) BspEI 992 TCCGGA T^CCGGA BspHI 806 TCATGA T^CATGA BsrDI 986 GCAATG GCAATG(2/0) BssHII 876 GCGCGC G^CGCGC BstEII 1137 GGTCACC G^GT[ACGT]ACC BstPI 1137 GGTCACC G^GT[ACGT]ACC Bsu36I 513 CCTGAGG CC^T[ACGT]AGG Eco81I 513 CCTGAGG CC^T[ACGT]AGG EcoO65I 1137 GGTCACC G^GT[ACGT]ACC EcoRI 1510 GAATTC G^AATTC FauI 126 CCCGC CCCGC(4/6) KpnI 827 GGTACC GGTAC^C MamI 798 GATGACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MroI 992 TCCGGA T^CCGGA NaeI 249 GCCGGC GCC^GGC NcoI 551 CCATGG C^CATGG NgoMIV 249 GCCGGC G^CCGGC PshAI 784 GACCTCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PvuII 1384 CAGCTG CAG^CTG RcaI 806 TCATGA T^CATGA SapI 432 GCTCTTC GCTCTTC(1/4) SbfI 1089 CCTGCAGG CCTGCA^GG SmaI 89 CCCGGG CCC^GGG Sse8387I 1089 CCTGCAGG CCTGCA^GG TfiI 720 GATTC G^A[AT]TC XmaCI 89 CCCGGG C^CCGGG XmaI 89 CCCGGG C^CCGGG XmnI 1510 GAATTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AccI 940 GTCTAC GT^[AC][GT]AC 1435 GTCTAC AcsI 1510 GAATTC [GA]^AATT[CT] 1522 GAATTT AlwNI 337 CAGTACCTG CAG[ACGT][ACGT][ACGT]^CTG 439 CAGCCCCTG ApoI 1510 GAATTC [GA]^AATT[CT] 1522 GAATTT BalI 29 TGGCCA TGG^CCA 107 TGGCCA BanI 827 GGTACC G^G[CT][GA]CC 1462 GGTGCC BsaI 19 GAGACC GGTCTC(1/5) 1330 GAGACC BsaWI 992 TCCGGA [AT]^CCGG[AT] 1217 ACCGGA BsrFI 249 GCCGGC [GA]^CCGG[CT] 634 ACCGGC BstYI 419 AGATCC [GA]^GATC[CT] 995 GGATCT Cfr10I 249 GCCGGC [GA]^CCGG[CT] 634 ACCGGC DrdI 574 GACTTCAGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 982 GACGGCAATGTC MaeIII 818 GTGAC ^GT[ACGT]AC 1138 GTCAC MflI 419 AGATCC [GA]^GATC[CT] 995 GGATCT MluNI 29 TGGCCA TGG^CCA 107 TGGCCA MscI 29 TGGCCA TGG^CCA 107 TGGCCA MseI 401 TTAA T^TAA 1373 TTAA MspA1I 127 CCGCTG C[AC]G^C[GT]G 1384 CAGCTG PleI 1423 GACTC GAGTC(4/5) 142 GAGTC SacI 259 GAGCTC GAGCT^C 1084 GAGCTC SmlI 115 CTCAAG C^T[CT][GA]AG 952 CTCAAG TaqI 275 TCGA T^CGA 1568 TCGA Tru9I 401 TTAA T^TAA 1373 TTAA Tsp45I 818 GTGAC ^GT[GC]AC 1138 GTCAC Tsp509I 1511 AATT ^AATT 1523 AATT TspRI 526 CAGTG CA[GC]TG[ACGT][ACGT]^ 1530 CACTG TthHB8I 275 TCGA T^CGA 1568 TCGA XhoII 419 AGATCC [GA]^GATC[CT] 995 GGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 77 enzymes) # ######################################### AccII AciI AfaI AluI AlwI AspHI AvaI AvaII BanII BbvI BcnI BmyI BpmI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoNI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeII MboI MboII MnlI MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PflMI PpuMI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TseI Van91I