(Circular) (Six-base) MAPSORT of: pFN21AE3241.seq Check: 6457 from: 1 to: 6394 pFN21AE3241 6394 bp With 182 enzymes: SgfI * March 28, 2012 12:52 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2259 2934 2259 Size: 675 5719 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4204 278 Size: 53 83 186 936 2668 2468 Fragments arranged by size: 2668 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2946 3441 3737 1937 Size: 1009 495 296 4594 Fragments arranged by size: 4594 1009 495 296 Acc65I G'GTAC_C Cuts at: 2832 3715 2832 Size: 883 5511 AclI AA'CG_TT Cuts at: 1563 4521 4894 1563 Size: 2958 373 3063 Fragments arranged by size: 3063 2958 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2566 2702 3236 4457 5841 6109 1632 Size: 934 136 534 1221 1384 268 1917 Fragments arranged by size: 1917 1384 1221 934 534 268 136 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6197 AflIII A'CryG_T Cuts at: 1203 3600 5293 6270 1203 Size: 2397 1693 977 1327 Fragments arranged by size: 2397 1693 1327 977 AgeI A'CCGG_T Cuts at: 5225 5225 Size: 6394 AhdI GACnn_n'nnGTC Cuts at: 5123 5123 Size: 6394 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6394 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6394 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2555 2587 2555 Size: 32 6362 AlwNI CAG_nnn'CTG Cuts at: 2347 2449 4137 5709 2347 Size: 102 1688 1572 3032 Fragments arranged by size: 3032 1688 1572 102 ApaLI G'TGCA_C Cuts at: 4451 5607 4451 Size: 1156 5238 ApoI r'AATT_y Cuts at: 1493 3515 3527 3703 3929 4081 6190 1493 Size: 2022 12 176 226 152 2109 1697 Fragments arranged by size: 2109 2022 1697 226 176 152 12 AseI AT'TA_AT Cuts at: 160 1032 4948 160 Size: 872 3916 1606 Fragments arranged by size: 3916 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6394 AvaI C'yCGr_G Cuts at: 1870 1943 2058 2094 2963 3086 3719 1870 Size: 73 115 36 869 123 633 4545 Fragments arranged by size: 4545 869 633 123 115 73 36 BamHI G'GATC_C Cuts at: 3724 4091 3724 Size: 367 6027 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2832 3467 3715 5170 618 Size: 325 217 627 1045 635 248 1455 1842 Fragments arranged by size: 1842 1455 1045 635 627 325 248 217 BanII G_rGCy'C Cuts at: 1391 2268 2439 3093 3187 3240 3656 3713 1391 Size: 877 171 654 94 53 416 57 4072 Fragments arranged by size: 4072 877 654 416 171 94 57 53 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5423 BbvCI CC'TCA_GC Cuts at: 2165 2165 Size: 6394 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2734 2768 4585 4619 1903 Size: 34 797 34 1817 34 3678 Fragments arranged by size: 3678 1817 797 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4286 5496 4286 Size: 1210 5184 BclI T'GATC_A Cuts at: 1548 1548 Size: 6394 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2465 5005 136 Size: 107 122 71 631 605 793 2540 1525 Fragments arranged by size: 2540 1525 793 631 605 122 107 71 BglII A'GATC_T Cuts at: 6389 6389 Size: 6394 BlpI GC'TnA_GC Cuts at: 3811 3811 Size: 6394 Bme1580I G_kGCm'C Cuts at: 1790 3358 4455 5611 1790 Size: 1568 1097 1156 2573 Fragments arranged by size: 2573 1568 1156 1097 BmgBI CAC'GTC Cuts at: 1318 3463 1318 Size: 2145 4249 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2335 4125 5083 Size: 416 53 83 399 24 918 1790 958 Cuts at: 5083 442 Size: 1753 Fragments arranged by size: 1790 1753 958 918 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6394 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2192 2282 2294 2939 3317 3403 3413 3640 Size: 953 90 12 645 378 86 10 227 Cuts at: 3640 5054 1239 Size: 1414 2579 Fragments arranged by size: 2579 1414 953 645 378 227 90 86 12 10 Bpu10I CC'TnA_GC Cuts at: 2165 2165 Size: 6394 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2105 2942 4519 5384 5682 2105 Size: 837 1577 865 298 2817 Fragments arranged by size: 2817 1577 865 837 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2018 3329 5057 882 Size: 617 519 1311 1728 2219 Fragments arranged by size: 2219 1728 1311 617 519 BsaAI yAC'GTr Cuts at: 493 1204 4185 493 Size: 711 2981 2702 Fragments arranged by size: 2981 2702 711 BsaBI GATnn'nnATC Cuts at: 2807 6388 2807 Size: 3581 2813 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4201 4583 5218 275 Size: 53 83 186 936 2668 382 635 1451 Fragments arranged by size: 2668 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2997 3222 4827 5225 5499 5646 6057 2997 Size: 225 1605 398 274 147 411 3334 Fragments arranged by size: 3334 1605 411 398 274 225 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2555 2585 3101 3131 3183 Size: 30 165 30 1152 30 516 30 52 Cuts at: 3183 3213 1178 Size: 30 4359 Fragments arranged by size: 4359 1152 516 165 52 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2870 3119 3578 3652 1186 Size: 1684 249 459 74 3928 Fragments arranged by size: 3928 1684 459 249 74 BseYI C'CCAG_C Cuts at: 2550 3398 5597 6093 2550 Size: 848 2199 496 2851 Fragments arranged by size: 2851 2199 848 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2912 1163 Size: 573 1176 4645 Fragments arranged by size: 4645 1176 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2640 3844 4605 5633 1540 Size: 275 186 639 1204 761 1028 2301 Fragments arranged by size: 2301 1204 1028 761 639 275 186 BsiHKAI G_wGCw'C Cuts at: 2268 2925 3093 4455 4540 5611 2268 Size: 657 168 1362 85 1071 3051 Fragments arranged by size: 3051 1362 1071 657 168 85 BsmI GAATG_Cn' Cuts at: 3905 3998 3905 Size: 93 6301 BsmBI CGTCTCn'nnnn_ Cuts at: 3569 3569 Size: 6394 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2268 2439 2925 3093 3187 3240 3358 Size: 399 478 171 486 168 94 53 118 Cuts at: 3358 3656 3713 4455 4540 5611 1391 Size: 298 57 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 486 478 399 298 171 168 118 94 85 57 53 BspEI T'CCGG_A Cuts at: 2997 6057 2997 Size: 3060 3334 BspHI T'CATG_A Cuts at: 2811 4283 6013 2811 Size: 1472 1730 3192 Fragments arranged by size: 3192 1730 1472 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2259 2736 2934 3347 3749 844 Size: 1415 477 198 413 402 3489 Fragments arranged by size: 3489 1415 477 413 402 198 BsrBI CCG'CTC Cuts at: 1394 1823 4281 1394 Size: 429 2458 3507 Fragments arranged by size: 3507 2458 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2998 4889 5063 65 Size: 1183 1750 1891 174 1396 Fragments arranged by size: 1891 1750 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2254 2639 5038 5225 1700 Size: 110 444 385 2399 187 2869 Fragments arranged by size: 2869 2399 444 385 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6394 BssHII G'CGCG_C Cuts at: 1925 2881 1925 Size: 956 5438 BssSI C'ACGA_G Cuts at: 4454 5466 4454 Size: 1012 5382 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6394 BstBI TT'CG_AA Cuts at: 5198 5198 Size: 6394 BstEII G'GTnAC_C Cuts at: 1184 3142 1184 Size: 1958 4436 BstYI r'GATC_y Cuts at: 1972 2424 3000 3724 4091 4476 4493 5945 6031 Size: 452 576 724 367 385 17 1452 86 Cuts at: 6031 6043 6389 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 724 576 452 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2519 2519 Size: 6394 BtgI C'CryG_G Cuts at: 513 1065 2004 2028 2556 3021 3120 3187 513 Size: 552 939 24 528 465 99 67 3720 Fragments arranged by size: 3720 939 552 528 465 99 67 24 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2035 487 Size: 683 56 809 4846 Fragments arranged by size: 4846 809 683 56 BtsI GCAGTG_nn' Cuts at: 2537 3910 4704 4724 2537 Size: 1373 794 20 4207 Fragments arranged by size: 4207 1373 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6394 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6359 DraI TTT'AAA Cuts at: 3698 3698 Size: 6394 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2585 2993 5401 809 Size: 507 1269 408 2408 1802 Fragments arranged by size: 2408 1802 1269 507 408 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2034 2112 3018 Size: 54 1425 50 275 18 204 78 906 Cuts at: 3018 3165 3841 4730 6359 8 Size: 147 676 889 1629 43 Fragments arranged by size: 1629 1425 906 889 676 275 204 147 78 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3841 1537 Size: 275 2029 4090 Fragments arranged by size: 4090 2029 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2372 2444 2681 2894 3371 4324 1353 Size: 1019 72 237 213 477 953 3423 Fragments arranged by size: 3423 1019 953 477 237 213 72 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2953 4981 5355 5501 387 Size: 1064 138 1364 2028 374 146 1280 Fragments arranged by size: 2028 1364 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2266 3091 2266 Size: 825 5569 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2192 2282 2294 2566 2702 2939 3236 Size: 393 560 90 12 272 136 237 297 Cuts at: 3236 3317 3403 3413 3640 4457 5054 5841 6109 Size: 81 86 10 227 817 597 787 268 Cuts at: 6109 1239 Size: 1524 Fragments arranged by size: 1524 817 787 597 560 393 297 272 268 237 227 136 90 86 81 12 10 EcoNI CCTnn'n_nnAGG Cuts at: 2384 2453 3368 2384 Size: 69 915 5410 Fragments arranged by size: 5410 915 69 EcoO109I rG'GnC_Cy Cuts at: 2414 2587 3066 3173 3304 3373 2414 Size: 173 479 107 131 69 5435 Fragments arranged by size: 5435 479 173 131 107 69 EcoRI G'AATT_C Cuts at: 3515 3703 4081 3515 Size: 188 378 5828 Fragments arranged by size: 5828 378 188 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2233 2233 Size: 6394 FspI TGC'GCA Cuts at: 4900 4900 Size: 6394 HaeII r_GCGC'y Cuts at: 1678 3293 3319 3610 5541 1678 Size: 1615 26 291 1931 2531 Fragments arranged by size: 2531 1931 1615 291 26 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2000 2032 2811 2843 5035 Size: 32 135 32 428 32 779 32 2192 Cuts at: 5035 5067 5109 5141 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2192 779 428 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3738 3984 669 Size: 988 281 1800 246 3079 Fragments arranged by size: 3079 1800 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6394 HpaI GTT'AAC Cuts at: 3984 3984 Size: 6394 KpnI G_GTAC'C Cuts at: 2836 3719 2836 Size: 883 5511 MfeI C'AATT_G Cuts at: 3993 3993 Size: 6394 MluI A'CGCG_T Cuts at: 6270 6270 Size: 6394 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5508 5692 1305 Size: 4203 184 2007 Fragments arranged by size: 4203 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2036 2114 10 Size: 54 1425 343 204 78 4290 Fragments arranged by size: 4290 1425 343 204 78 54 MslI CAynn'nnrTG Cuts at: 518 1166 4352 4711 4870 518 Size: 648 3186 359 159 2042 Fragments arranged by size: 3186 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2134 3391 3810 4487 5635 5880 1606 Size: 528 1257 419 677 1148 245 2120 Fragments arranged by size: 2120 1257 1148 677 528 419 245 NaeI GCC'GGC Cuts at: 2256 2256 Size: 6394 NcoI C'CATG_G Cuts at: 513 1065 2004 2556 513 Size: 552 939 552 4351 Fragments arranged by size: 4351 939 552 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6394 NgoMIV G'CCGG_C Cuts at: 2254 2254 Size: 6394 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6394 NotI GC'GGCC_GC Cuts at: 3841 3841 Size: 6394 NspI r_CATG'y Cuts at: 1132 1229 3752 5297 1132 Size: 97 2523 1545 2229 Fragments arranged by size: 2523 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 3136 1348 Size: 1788 4606 PciI A'CATG_T Cuts at: 5293 5293 Size: 6394 PflMI CCAn_nnn'nTGG Cuts at: 1108 2347 2557 3555 1108 Size: 1239 210 998 3947 Fragments arranged by size: 3947 1239 998 210 PfoI T'CCnGG_A Cuts at: 2379 2427 2490 2574 2871 2379 Size: 48 63 84 297 5902 Fragments arranged by size: 5902 297 84 63 48 PmeI GTTT'AAAC Cuts at: 3698 3698 Size: 6394 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2555 2587 4463 4495 5976 6008 2555 Size: 32 1876 32 1481 32 2941 Fragments arranged by size: 2941 1876 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2587 3066 3173 3304 3373 2587 Size: 479 107 131 69 5608 Fragments arranged by size: 5608 479 131 107 69 PshAI GACnn'nnGTC Cuts at: 1613 2793 1613 Size: 1180 5214 PsiI TTA'TAA Cuts at: 3964 3964 Size: 6394 PstI C_TGCA'G Cuts at: 830 2375 2457 2532 3099 3746 830 Size: 1545 82 75 567 647 3478 Fragments arranged by size: 3478 1545 647 567 82 75 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6394 PvuII CAG'CTG Cuts at: 3391 3391 Size: 6394 SacI G_AGCT'C Cuts at: 2268 3093 2268 Size: 825 5569 SalI G'TCGA_C Cuts at: 1936 3736 1936 Size: 1800 4594 SanDI GG'GwC_CC Cuts at: 3066 3066 Size: 6394 SapI GCTCTTCn'nnn_ Cuts at: 2444 2444 Size: 6394 SbfI CC_TGCA'GG Cuts at: 3099 3746 3099 Size: 647 5747 ScaI AGT'ACT Cuts at: 4642 6354 4642 Size: 1712 4682 SfcI C'TryA_G Cuts at: 826 1045 2371 2453 2528 3095 3742 4877 5558 Size: 219 1326 82 75 567 647 1135 681 Cuts at: 5558 5749 826 Size: 191 1471 Fragments arranged by size: 1471 1326 1135 681 647 567 219 191 82 75 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6394 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6394 SmaI CCC'GGG Cuts at: 1872 2096 3721 1872 Size: 224 1625 4545 Fragments arranged by size: 4545 1625 224 SmlI C'TyrA_G Cuts at: 820 1017 1943 2120 2957 4498 5399 5661 820 Size: 197 926 177 837 1541 901 262 1553 Fragments arranged by size: 1553 1541 926 901 837 262 197 177 SnaBI TAC'GTA Cuts at: 493 4185 493 Size: 3692 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6394 SphI G_CATG'C Cuts at: 1132 3752 1132 Size: 2620 3774 SspI AAT'ATT Cuts at: 5 52 4318 5 Size: 47 4266 2081 Fragments arranged by size: 4266 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6394 StyI C'CwwG_G Cuts at: 513 1065 2004 2556 3010 3051 3833 513 Size: 552 939 552 454 41 782 3074 Fragments arranged by size: 3074 939 782 552 552 454 41 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3455 4421 4438 4591 4776 5221 325 Size: 1255 1875 966 17 153 185 445 1498 Fragments arranged by size: 1875 1498 1255 966 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2511 2737 4640 Size: 274 80 33 51 1443 534 226 1903 Cuts at: 4640 6352 96 Size: 1712 138 Fragments arranged by size: 1903 1712 1443 534 274 226 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3183 3215 6354 6386 3183 Size: 32 3139 32 3191 Fragments arranged by size: 3191 3139 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6394 XbaI T'CTAG_A Cuts at: 3730 3730 Size: 6394 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6394 XmaI C'CCGG_G Cuts at: 1870 2094 3719 1870 Size: 224 1625 4545 Fragments arranged by size: 4545 1625 224 XmnI GAAnn'nnTTC Cuts at: 3519 4523 3519 Size: 1004 5390 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4202 276 Size: 53 83 186 936 2668 2468 Fragments arranged by size: 2668 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BstXI BstZ17I DraIII EcoRV FseI FspAI KasI NarI NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacII SexAI SfiI SfoI SrfI SwaI XcmI