################################## # NO CUT ENZYMES ( 144 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AciI AclI AcsI AflII AgeI AhdI Alw44I AlwI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BamHI BbeI BbrPI BbsI BcgI BclI BfrI BglI BlnI BlpI Bpu1102I BpuAI BsaAI BsaJI BsaWI BseAI BseRI BsiEI BsiWI BsmBI BsmI BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BssHII Bst1107I BstBI BstUI BstXI BstZ17I CelII CfoI Cfr10I ClaI CpoI DraI DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoRI EcoRV EcoT14I EcoT22I FauI FbaI FseI FspI HaeII HhaI HinP1I HindIII HpaI KasI KpnI KspI MfeI MluI MroI MspA1I MunI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PflFI PflMI PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI PvuII RcaI RsrII SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I StyI SwaI Tth111I Van91I XbaI XcmI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### AcyI 698 GGCGTC G[GA]^CG[CT]C AflIII 752 ACACGT A^C[GA][CT]GT AocI 28 CCTCAGG CC^T[ACGT]AGG AvaI 281 CTCGAG C^[CT]CG[GA]G BalI 915 TGGCCA TGG^CCA BanI 872 GGCACC G^G[CT][GA]CC BbvCI 40 GCTGAGG CCTCAGC(-5/-2) BciVI 1205 GGATAC GTATCC(6/5) BcnI 401 CCCGG CC^[GC]GG BsaBI 588 GATGTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 698 GGCGTC G[GA]^CG[CT]C BsgI 657 GTGCAG GTGCAG(16/14) BsmFI 1120 GTCCC GGGAC(10/14) BsoBI 281 CTCGAG C^[CT]CG[GA]G BssSI 785 CTCGTG CACGAG(-5/-1) BstAPI 1252 GCAGTCAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 1177 GGTCACC G^GT[ACGT]ACC BstF5I 149 GGATG GGATG(2/0) BstPI 1177 GGTCACC G^GT[ACGT]ACC Bsu36I 28 CCTCAGG CC^T[ACGT]AGG DraII 581 AGGTCCT [GA]G^G[ACGT]CC[CT] DraIII 874 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG EaeI 915 TGGCCA [CT]^GGCC[GA] Eco81I 28 CCTCAGG CC^T[ACGT]AGG EcoO109I 581 AGGTCCT [GA]G^G[ACGT]CC[CT] EcoO65I 1177 GGTCACC G^GT[ACGT]ACC FokI 149 GGATG GGATG(9/13) HapII 402 CCGG C^CGG HgaI 699 GCGTC GACGC(5/10) Hin1I 698 GGCGTC G[GA]^CG[CT]C HincII 930 GTTGAC GT[CT]^[GA]AC HindII 930 GTTGAC GT[CT]^[GA]AC HpaII 402 CCGG C^CGG MamI 588 GATGTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluNI 915 TGGCCA TGG^CCA MscI 915 TGGCCA TGG^CCA MslI 561 CATATCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspI 402 CCGG C^CGG NciI 401 CCCGG CC^[GC]GG NlaIV 872 GGCACC GG[ACGT]^[ACGT]CC PaeR7I 281 CTCGAG C^TCGAG PleI 511 GAGTC GAGTC(4/5) PpuMI 581 AGGTCCT [GA]G^G[AT]CC[CT] PshAI 1248 GACTGCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SapI 943 GAAGAGC GCTCTTC(1/4) SfaNI 994 GATGC GCATC(5/9) SspI 884 AATATT AAT^ATT StuI 1333 AGGCCT AGG^CCT XhoI 281 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AlwNI 35 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG 726 CAGAAGCTG AspHI 140 GAGCTC G[AT]GC[AT]^C 323 GAGCTC AvaII 582 GGTCC G^G[AT]CC 624 GGACC BanII 140 GAGCTC G[GA]GC[CT]^C 323 GAGCTC BglII 111 AGATCT A^GATCT 471 AGATCT BmyI 140 GAGCTC G[AGT]GC[ACT]^C 323 GAGCTC BsaI 683 GAGACC GGTCTC(1/5) 404 GGTCTC BsiHKAI 140 GAGCTC G[AT]GC[AT]^C 323 GAGCTC Bsp1286I 140 GAGCTC G[AGT]GC[ACT]^C 323 GAGCTC BstYI 111 AGATCT [GA]^GATC[CT] 471 AGATCT Cac8I 117 GCAGGC GC[ACGT]^[ACGT]GC 706 GCCTGC Cfr13I 582 GGTCC G^G[ACGT]CC 624 GGACC EarI 289 CTCTTC CTCTTC(1/4) 943 GAAGAG Ksp632I 289 CTCTTC CTCTTC(1/4) 943 GAAGAG MaeII 754 ACGT A^CGT 966 ACGT MflI 111 AGATCT [GA]^GATC[CT] 471 AGATCT MwoI 642 GCAGTTGTCGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 1252 GCAGTCAGTGC NlaIII 919 CATG CATG^ 1150 CATG PstI 115 CTGCAG CTGCA^G 1250 CTGCAG SacI 140 GAGCTC GAGCT^C 323 GAGCTC Sau96I 582 GGTCC G^G[ACGT]CC 624 GGACC SmlI 281 CTCGAG C^T[CT][GA]AG 541 CTTGAG TaqI 195 TCGA T^CGA 282 TCGA Tsp509I 184 AATT ^AATT 999 AATT TthHB8I 195 TCGA T^CGA 282 TCGA XhoII 111 AGATCT [GA]^GATC[CT] 471 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 39 enzymes) # ######################################### AfaI AluI BbvI BfaI BpmI BsiYI BslI BsmAI Bsp1407I BsrGI BsrI BssKI BstNI DdeI DpnII EcoRII Fnu4HI HaeIII HinfI HphI ItaI MaeI MaeIII MboI MboII MnlI MseI MvaI NdeII RsaI Sau3AI ScrFI SfcI SspBI TfiI Tru9I TseI Tsp45I TspRI