(Circular) (Six-base) MAPSORT of: pFN21AE3192.seq Check: 1287 from: 1 to: 6046 pFN21AE3192 6046 bp With 182 enzymes: SgfI * March 28, 2012 12:52 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3856 278 Size: 53 83 186 936 2320 2468 Fragments arranged by size: 2468 2320 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3389 1937 Size: 1452 4594 Acc65I G'GTAC_C Cuts at: 3367 3367 Size: 6046 AclI AA'CG_TT Cuts at: 1563 4173 4546 1563 Size: 2610 373 3063 Fragments arranged by size: 3063 2610 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2967 4109 5493 5761 1632 Size: 1335 1142 1384 268 1917 Fragments arranged by size: 1917 1384 1335 1142 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5849 AflIII A'CryG_T Cuts at: 1203 2757 4945 5922 1203 Size: 1554 2188 977 1327 Fragments arranged by size: 2188 1554 1327 977 AgeI A'CCGG_T Cuts at: 4877 4877 Size: 6046 AhdI GACnn_n'nnGTC Cuts at: 4775 4775 Size: 6046 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6046 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6046 AlwNI CAG_nnn'CTG Cuts at: 2045 2736 3789 5361 2045 Size: 691 1053 1572 2730 Fragments arranged by size: 2730 1572 1053 691 ApaLI G'TGCA_C Cuts at: 4103 5259 4103 Size: 1156 4890 ApoI r'AATT_y Cuts at: 1493 3355 3581 3733 5842 1493 Size: 1862 226 152 2109 1697 Fragments arranged by size: 2109 1862 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4600 160 Size: 872 3568 1606 Fragments arranged by size: 3568 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6046 AvaI C'yCGr_G Cuts at: 1870 1943 2286 3371 1870 Size: 73 343 1085 4545 Fragments arranged by size: 4545 1085 343 73 BamHI G'GATC_C Cuts at: 3376 3743 3376 Size: 367 5679 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2877 3367 4822 618 Size: 325 217 627 1090 490 1455 1842 Fragments arranged by size: 1842 1455 1090 627 490 325 217 BanII G_rGCy'C Cuts at: 1391 2149 2332 3365 1391 Size: 758 183 1033 4072 Fragments arranged by size: 4072 1033 758 183 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5075 BbvCI CC'TCA_GC Cuts at: 2046 2046 Size: 6046 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4237 4271 1903 Size: 34 50 34 2216 34 3678 Fragments arranged by size: 3678 2216 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3204 3938 5148 3204 Size: 734 1210 4102 Fragments arranged by size: 4102 1210 734 BclI T'GATC_A Cuts at: 1548 1548 Size: 6046 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4657 136 Size: 107 122 71 631 605 2985 1525 Fragments arranged by size: 2985 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2116 2476 6041 2116 Size: 360 3565 2121 Fragments arranged by size: 3565 2121 360 BlpI GC'TnA_GC Cuts at: 3463 3463 Size: 6046 Bme1580I G_kGCm'C Cuts at: 1790 4107 5263 1790 Size: 2317 1156 2573 Fragments arranged by size: 2573 2317 1156 BmgBI CAC'GTC Cuts at: 1318 2760 1318 Size: 1442 4604 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3020 3777 4735 Size: 416 53 83 399 24 1603 757 958 Cuts at: 4735 442 Size: 1753 Fragments arranged by size: 1753 1603 958 757 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6046 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2243 2243 Size: 6046 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2117 2154 2213 2397 2785 4706 1239 Size: 878 37 59 184 388 1921 2579 Fragments arranged by size: 2579 1921 878 388 184 59 37 Bpu10I CC'TnA_GC Cuts at: 2046 2541 2046 Size: 495 5551 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2567 4171 5036 5334 2567 Size: 1604 865 298 3279 Fragments arranged by size: 3279 1604 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2415 2682 4709 882 Size: 617 916 267 2027 2219 Fragments arranged by size: 2219 2027 916 617 267 BsaAI yAC'GTr Cuts at: 493 1204 3837 493 Size: 711 2633 2702 Fragments arranged by size: 2702 2633 711 BsaBI GATnn'nnATC Cuts at: 2597 6040 2597 Size: 3443 2603 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2704 3853 4235 4870 Size: 53 83 186 936 1171 1149 382 635 Cuts at: 4870 275 Size: 1451 Fragments arranged by size: 1451 1171 1149 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4479 4877 5151 5298 5709 4479 Size: 398 274 147 411 4816 Fragments arranged by size: 4816 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2395 2425 2636 2666 1178 Size: 30 165 30 992 30 211 30 4558 Fragments arranged by size: 4558 992 211 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6046 BseYI C'CCAG_C Cuts at: 5249 5745 5249 Size: 496 5550 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2683 1163 Size: 573 947 4526 Fragments arranged by size: 4526 947 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3496 4257 5285 1540 Size: 275 186 1495 761 1028 2301 Fragments arranged by size: 2301 1495 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2149 2332 4107 4192 5263 2149 Size: 183 1775 85 1071 2932 Fragments arranged by size: 2932 1775 1071 183 85 BsmI GAATG_Cn' Cuts at: 3557 3650 3557 Size: 93 5953 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2149 2332 3365 4107 4192 5263 1391 Size: 399 359 183 1033 742 85 1071 2174 Fragments arranged by size: 2174 1071 1033 742 399 359 183 85 BspEI T'CCGG_A Cuts at: 5709 5709 Size: 6046 BspHI T'CATG_A Cuts at: 3935 5665 3935 Size: 1730 4316 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3401 844 Size: 2557 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3933 1394 Size: 429 2110 3507 Fragments arranged by size: 3507 2110 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4541 4715 65 Size: 1183 3293 174 1396 Fragments arranged by size: 3293 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4690 4877 1700 Size: 110 2880 187 2869 Fragments arranged by size: 2880 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2595 3130 3285 96 Size: 2499 535 155 2857 Fragments arranged by size: 2857 2499 535 155 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6046 BssSI C'ACGA_G Cuts at: 2790 4106 5118 2790 Size: 1316 1012 3718 Fragments arranged by size: 3718 1316 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3263 794 Size: 2469 3577 BstBI TT'CG_AA Cuts at: 4850 4850 Size: 6046 BstEII G'GTnAC_C Cuts at: 1184 3182 1184 Size: 1998 4048 BstYI r'GATC_y Cuts at: 1972 2116 2476 3376 3743 4128 4145 5597 5683 Size: 144 360 900 367 385 17 1452 86 Cuts at: 5683 5695 6041 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 900 385 367 360 346 144 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2034 2034 Size: 6046 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5494 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5307 Fragments arranged by size: 5307 683 56 BtsI GCAGTG_nn' Cuts at: 3562 4356 4376 3562 Size: 794 20 5232 Fragments arranged by size: 5232 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6046 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6011 DraI TTT'AAA Cuts at: 3350 3350 Size: 6046 DraIII CAC_nnn'GTG Cuts at: 2884 2884 Size: 6046 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5053 809 Size: 507 3737 1802 Fragments arranged by size: 3737 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2920 3493 4382 Size: 54 1425 50 275 18 1090 573 889 Cuts at: 4382 6011 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1090 889 573 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3493 1537 Size: 275 1681 4090 Fragments arranged by size: 4090 1681 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2300 2943 3976 1353 Size: 947 643 1033 3423 Fragments arranged by size: 3423 1033 947 643 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4633 5007 5153 387 Size: 1064 138 3044 374 146 1280 Fragments arranged by size: 3044 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2147 2330 2147 Size: 183 5863 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2117 2154 2213 2397 2785 2967 4109 Size: 393 485 37 59 184 388 182 1142 Cuts at: 4109 4706 5493 5761 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1142 787 597 485 393 388 268 184 182 59 37 EcoO109I rG'GnC_Cy Cuts at: 2587 2587 Size: 6046 EcoRI G'AATT_C Cuts at: 3355 3733 3355 Size: 378 5668 FspI TGC'GCA Cuts at: 4552 4552 Size: 6046 HaeII r_GCGC'y Cuts at: 1678 5193 1678 Size: 3515 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2468 2500 2621 2636 2653 Size: 32 135 32 896 32 121 15 17 Cuts at: 2653 2661 2668 2693 2942 2974 4687 4719 4761 Size: 8 7 25 249 32 1713 32 42 Cuts at: 4761 4793 1373 Size: 32 2626 Fragments arranged by size: 2626 1713 896 249 135 121 42 32 32 32 32 32 32 25 17 15 8 7 HincII GTy'rAC Cuts at: 669 1657 1938 2937 3390 3636 669 Size: 988 281 999 453 246 3079 Fragments arranged by size: 3079 999 988 453 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6046 HpaI GTT'AAC Cuts at: 3636 3636 Size: 6046 KpnI G_GTAC'C Cuts at: 3371 3371 Size: 6046 MfeI C'AATT_G Cuts at: 3645 3645 Size: 6046 MluI A'CGCG_T Cuts at: 5922 5922 Size: 6046 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2410 5160 5344 1305 Size: 1105 2750 184 2007 Fragments arranged by size: 2750 2007 1105 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2922 10 Size: 54 1425 343 1090 3134 Fragments arranged by size: 3134 1425 1090 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2570 4004 4363 4522 518 Size: 648 1404 1434 359 159 2042 Fragments arranged by size: 2042 1434 1404 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3462 4139 5287 5532 1606 Size: 1856 677 1148 245 2120 Fragments arranged by size: 2120 1856 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5494 NdeI CA'TA_TG Cuts at: 387 387 Size: 6046 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6046 NotI GC'GGCC_GC Cuts at: 3493 3493 Size: 6046 NspI r_CATG'y Cuts at: 1132 1229 3404 4949 1132 Size: 97 2175 1545 2229 Fragments arranged by size: 2229 2175 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6046 PciI A'CATG_T Cuts at: 4945 4945 Size: 6046 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6046 PfoI T'CCnGG_A Cuts at: 2762 2762 Size: 6046 PmeI GTTT'AAAC Cuts at: 3350 3350 Size: 6046 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4115 4147 5628 5660 4115 Size: 32 1481 32 4501 Fragments arranged by size: 4501 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2587 2587 Size: 6046 PshAI GACnn'nnGTC Cuts at: 1613 3257 1613 Size: 1644 4402 PsiI TTA'TAA Cuts at: 3616 3616 Size: 6046 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2971 3003 2971 Size: 32 6014 PstI C_TGCA'G Cuts at: 830 2124 3259 3398 830 Size: 1294 1135 139 3478 Fragments arranged by size: 3478 1294 1135 139 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6046 SacI G_AGCT'C Cuts at: 2149 2332 2149 Size: 183 5863 SalI G'TCGA_C Cuts at: 1936 3388 1936 Size: 1452 4594 SapI GCTCTTCn'nnn_ Cuts at: 2943 2943 Size: 6046 SbfI CC_TGCA'GG Cuts at: 3398 3398 Size: 6046 ScaI AGT'ACT Cuts at: 4294 6006 4294 Size: 1712 4334 SfcI C'TryA_G Cuts at: 826 1045 2120 2866 3164 3255 3394 4529 5210 Size: 219 1075 746 298 91 139 1135 681 Cuts at: 5210 5401 826 Size: 191 1471 Fragments arranged by size: 1471 1135 1075 746 681 298 219 191 139 91 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6046 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6046 SmaI CCC'GGG Cuts at: 1872 3373 1872 Size: 1501 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2286 2546 4150 5051 5313 820 Size: 197 926 343 260 1604 901 262 1553 Fragments arranged by size: 1604 1553 926 901 343 262 260 197 SnaBI TAC'GTA Cuts at: 493 3837 493 Size: 3344 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6046 SphI G_CATG'C Cuts at: 1132 3404 1132 Size: 2272 3774 SspI AAT'ATT Cuts at: 5 52 2891 3970 5 Size: 47 2839 1079 2081 Fragments arranged by size: 2839 2081 1079 47 StuI AGG'CCT Cuts at: 1517 3340 1517 Size: 1823 4223 StyI C'CwwG_G Cuts at: 513 1065 3485 513 Size: 552 2420 3074 Fragments arranged by size: 3074 2420 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2599 4073 4090 4243 4428 4873 325 Size: 1255 1019 1474 17 153 185 445 1498 Fragments arranged by size: 1498 1474 1255 1019 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2595 3130 3285 Size: 274 80 33 51 1443 618 535 155 Cuts at: 3285 4292 6004 96 Size: 1007 1712 138 Fragments arranged by size: 1712 1443 1007 618 535 274 155 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6006 6038 6006 Size: 32 6014 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6046 XbaI T'CTAG_A Cuts at: 3382 3382 Size: 6046 XhoI C'TCGA_G Cuts at: 1943 2286 1943 Size: 343 5703 XmaI C'CCGG_G Cuts at: 1870 3371 1870 Size: 1501 4545 XmnI GAAnn'nnTTC Cuts at: 4175 4175 Size: 6046 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3854 276 Size: 53 83 186 936 2320 2468 Fragments arranged by size: 2468 2320 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstXI BstZ17I EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PvuII RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI