################################## # NO CUT ENZYMES ( 154 enzymes) # ################################## AatII Acc65I AccI AclI AcsI AcyI AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AviII AvrII BalI BamHI BanI BbeI BbrPI BbsI BbvCI BbvI BcgI BclI BfaI BglII BlnI BlpI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsgI BsiHKAI BsiWI BsmI BsoBI BspHI BspLU11I BspMI BsrFI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I Bsu36I Cac8I CelII CfoI Cfr10I CpoI DraIII DrdI Eam1105I Eco47III Eco81I EcoO65I EcoRI EcoRV FbaI FseI FspI HaeII HhaI Hin1I HinP1I HindIII HphI KasI KpnI KspI MaeI MaeII MamI MfeI MluI MluNI MscI MslI MspA1I MunI NaeI NarI NcoI NdeI NgoMIV NheI NruI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfaNI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI StuI SwaI TseI Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 58 enzymes) # ################################### AccII 59 CGCG CG^CG AccIII 219 TCCGGA T^CCGGA AfaI 404 GTAC GT^AC AflII 322 CTTAAG C^TTAAG AluI 417 AGCT AG^CT AlwI 364 GATCC GGATC(4/5) BanII 38 GGGCTC G[GA]GC[CT]^C BciVI 610 GGATAC GTATCC(6/5) BcnI 305 CCGGG CC^[GC]GG BfrI 322 CTTAAG C^TTAAG BglI 226 GCCGTGACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BmyI 38 GGGCTC G[AGT]GC[ACT]^C BsaWI 219 TCCGGA [AT]^CCGG[AT] BseAI 219 TCCGGA T^CCGGA BseRI 313 GAGGAG GAGGAG(10/8) BsiEI 191 CGGCCG CG[GA][CT]^CG Bsp1286I 38 GGGCTC G[AGT]GC[ACT]^C Bsp1407I 403 TGTACA T^GTACA BspDI 596 ATCGAT AT^CGAT BspEI 219 TCCGGA T^CCGGA BsrBI 133 CCGCTC CCGCTC(-3/-3) BsrDI 470 GCAATG GCAATG(2/0) BsrGI 403 TGTACA T^GTACA BstF5I 580 GGATG GGATG(2/0) BstUI 59 CGCG CG^CG BstYI 363 AGATCC [GA]^GATC[CT] ClaI 596 ATCGAT AT^CGAT DraI 9 TTTAAA TTT^AAA DsaI 34 CCACGG C^C[GA][CT]GG EagI 191 CGGCCG C^GGCCG EarI 62 GAAGAG CTCTTC(1/4) EclXI 191 CGGCCG C^GGCCG Eco52I 191 CGGCCG C^GGCCG EcoNI 85 CCTATGCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoT14I 606 CCTTGG C^C[AT][AT]GG EcoT22I 1 ATGCAT ATGCA^T FauI 132 CCCGC CCCGC(4/6) FokI 580 GGATG GGATG(9/13) HgaI 379 GACGC GACGC(5/10) HincII 397 GTTAAC GT[CT]^[GA]AC HindII 397 GTTAAC GT[CT]^[GA]AC HpaI 397 GTTAAC GTT^AAC Ksp632I 62 GAAGAG CTCTTC(1/4) MflI 363 AGATCC [GA]^GATC[CT] MroI 219 TCCGGA T^CCGGA MvnI 59 CGCG CG^CG NciI 305 CCGGG CC^[GC]GG NlaIII 421 CATG CATG^ NotI 190 GCGGCCGC GC^GGCCGC NsiI 1 ATGCAT ATGCA^T RsaI 404 GTAC GT^AC SfiI 225 GGCCGTGACGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SmlI 322 CTTAAG C^T[CT][GA]AG SspBI 403 TGTACA T^GTACA StyI 606 CCTTGG C^C[AT][AT]GG TaqI 597 TCGA T^CGA TthHB8I 597 TCGA T^CGA XhoII 363 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### BsaI 17 GGTCTC GGTCTC(1/5) 177 GGTCTC BsmBI 261 CGTCTC CGTCTC(1/5) 65 GAGACG BsmFI 345 GGGAC GGGAC(10/14) 95 GTCCC DdeI 264 CTCAG C^T[ACGT]AG 763 CTGAG DpnII 364 GATC ^GATC 595 GATC Fnu4HI 190 GCGGC GC^[ACGT]GC 193 GCCGC HapII 220 CCGG C^CGG 305 CCGG HpaII 220 CCGG C^CGG 305 CCGG ItaI 190 GCGGC GC^[ACGT]GC 193 GCCGC MboI 364 GATC ^GATC 595 GATC MboII 51 GAAGA GAAGA(8/7) 62 GAAGA MspI 220 CCGG C^CGG 305 CCGG NdeII 364 GATC ^GATC 595 GATC PleI 239 GACTC GAGTC(4/5) 316 GAGTC Sau3AI 364 GATC ^GATC 595 GATC SfcI 272 CTACAG C^T[GA][CT]AG 644 CTGTAG TfiI 30 GATTC G^A[AT]TC 460 GATTC Tsp45I 229 GTGAC ^GT[GC]AC 268 GTCAC TspRI 465 CAGTG CA[GC]TG[ACGT][ACGT]^ 642 CACTG ######################################### # 3 OR MORE CUT ENZYMES ( 27 enzymes) # ######################################### AciI AvaII BsaJI BsiYI BslI BsmAI BsrI BssKI BstNI Cfr13I DraII EaeI EcoO109I EcoRII HaeIII HinfI MaeIII MnlI MseI MvaI MwoI NlaIV PpuMI Sau96I ScrFI Tru9I Tsp509I