(Circular) (Six-base) MAPSORT of: pFN21AE3102.seq Check: 3800 from: 1 to: 5512 pFN21AE3102 5512 bp With 182 enzymes: SgfI * March 28, 2012 12:51 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3322 278 Size: 53 83 186 936 1786 2468 Fragments arranged by size: 2468 1786 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2855 1937 Size: 918 4594 Acc65I G'GTAC_C Cuts at: 2833 2833 Size: 5512 AclI AA'CG_TT Cuts at: 1563 3639 4012 1563 Size: 2076 373 3063 Fragments arranged by size: 3063 2076 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2700 3575 4959 5227 1632 Size: 1068 875 1384 268 1917 Fragments arranged by size: 1917 1384 1068 875 268 AflII C'TTAA_G Cuts at: 820 1017 2327 820 Size: 197 1310 4005 Fragments arranged by size: 4005 1310 197 AflIII A'CryG_T Cuts at: 1203 4411 5388 1203 Size: 3208 977 1327 Fragments arranged by size: 3208 1327 977 AgeI A'CCGG_T Cuts at: 4343 4343 Size: 5512 AhdI GACnn_n'nnGTC Cuts at: 4241 4241 Size: 5512 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5512 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5512 AlwNI CAG_nnn'CTG Cuts at: 3255 4827 3255 Size: 1572 3940 ApaLI G'TGCA_C Cuts at: 3569 4725 3569 Size: 1156 4356 ApoI r'AATT_y Cuts at: 1493 2821 3047 3199 5308 1493 Size: 1328 226 152 2109 1697 Fragments arranged by size: 2109 1697 1328 226 152 AseI AT'TA_AT Cuts at: 160 1032 4066 160 Size: 872 3034 1606 Fragments arranged by size: 3034 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5512 AvaI C'yCGr_G Cuts at: 1870 1943 2837 1870 Size: 73 894 4545 Fragments arranged by size: 4545 894 73 BamHI G'GATC_C Cuts at: 2842 3209 2842 Size: 367 5145 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2833 4288 618 Size: 325 217 627 1046 1455 1842 Fragments arranged by size: 1842 1455 1046 627 325 217 BanII G_rGCy'C Cuts at: 1391 2047 2831 1391 Size: 656 784 4072 Fragments arranged by size: 4072 784 656 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4541 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3703 3737 1903 Size: 34 50 34 1682 34 3678 Fragments arranged by size: 3678 1682 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2609 3404 4614 2609 Size: 795 1210 3507 Fragments arranged by size: 3507 1210 795 BclI T'GATC_A Cuts at: 1548 1548 Size: 5512 BfrBI ATG'CAT Cuts at: 2008 2008 Size: 5512 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2237 4123 136 Size: 107 122 71 631 605 565 1886 1525 Fragments arranged by size: 1886 1525 631 605 565 122 107 71 BglII A'GATC_T Cuts at: 5507 5507 Size: 5512 BlpI GC'TnA_GC Cuts at: 2929 2929 Size: 5512 Bme1580I G_kGCm'C Cuts at: 1790 3573 4729 1790 Size: 1783 1156 2573 Fragments arranged by size: 2573 1783 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5512 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2502 3243 4201 Size: 416 53 83 399 24 1085 741 958 Cuts at: 4201 442 Size: 1753 Fragments arranged by size: 1753 1085 958 741 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5512 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4172 1239 Size: 2933 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3637 4502 4800 3637 Size: 865 298 4349 Fragments arranged by size: 4349 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2028 2188 4175 882 Size: 617 529 160 1987 2219 Fragments arranged by size: 2219 1987 617 529 160 BsaAI yAC'GTr Cuts at: 493 1204 3303 493 Size: 711 2099 2702 Fragments arranged by size: 2702 2099 711 BsaBI GATnn'nnATC Cuts at: 5506 5506 Size: 5512 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3319 3701 4336 275 Size: 53 83 186 936 1786 382 635 1451 Fragments arranged by size: 1786 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2224 3945 4343 4617 4764 5175 2224 Size: 1721 398 274 147 411 2561 Fragments arranged by size: 2561 1721 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5287 Fragments arranged by size: 5287 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2333 1186 Size: 1147 4365 BseYI C'CCAG_C Cuts at: 4715 5211 4715 Size: 496 5016 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4939 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2199 2962 3723 4751 1540 Size: 275 186 198 763 761 1028 2301 Fragments arranged by size: 2301 1028 763 761 275 198 186 BsiHKAI G_wGCw'C Cuts at: 3573 3658 4729 3573 Size: 85 1071 4356 Fragments arranged by size: 4356 1071 85 BsmI GAATG_Cn' Cuts at: 3023 3116 3023 Size: 93 5419 BsmBI CGTCTCn'nnnn_ Cuts at: 2064 2272 2064 Size: 208 5304 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2047 2831 3573 3658 4729 1391 Size: 399 257 784 742 85 1071 2174 Fragments arranged by size: 2174 1071 784 742 399 257 85 BspEI T'CCGG_A Cuts at: 2224 5175 2224 Size: 2951 2561 BspHI T'CATG_A Cuts at: 3401 5131 3401 Size: 1730 3782 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2867 844 Size: 2023 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2140 3399 1394 Size: 429 317 1259 3507 Fragments arranged by size: 3507 1259 429 317 BsrDI GCAATG_nn' Cuts at: 65 1248 2482 4007 4181 65 Size: 1183 1234 1525 174 1396 Fragments arranged by size: 1525 1396 1234 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4156 4343 1700 Size: 110 2346 187 2869 Fragments arranged by size: 2869 2346 187 110 BsrGI T'GTAC_A Cuts at: 96 2408 96 Size: 2312 3200 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5512 BssSI C'ACGA_G Cuts at: 3572 4584 3572 Size: 1012 4500 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5512 BstBI TT'CG_AA Cuts at: 4316 4316 Size: 5512 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5512 BstYI r'GATC_y Cuts at: 1972 2368 2842 3209 3594 3611 5063 5149 5161 Size: 396 474 367 385 17 1452 86 12 Cuts at: 5161 5507 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 474 396 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2039 513 Size: 552 974 3986 Fragments arranged by size: 3986 974 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4773 Fragments arranged by size: 4773 683 56 BtsI GCAGTG_nn' Cuts at: 3028 3822 3842 3028 Size: 794 20 4698 Fragments arranged by size: 4698 794 20 ClaI AT'CG_AT Cuts at: 1554 2602 1554 Size: 1048 4464 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5477 DraI TTT'AAA Cuts at: 2016 2816 2016 Size: 800 4712 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4519 809 Size: 507 3203 1802 Fragments arranged by size: 3203 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2196 2204 2238 Size: 54 1425 50 275 18 366 8 34 Cuts at: 2238 2959 3848 5477 8 Size: 721 889 1629 43 Fragments arranged by size: 1629 1425 889 721 366 275 54 50 43 34 18 8 EagI C'GGCC_G Cuts at: 1537 1812 2196 2959 1537 Size: 275 384 763 4090 Fragments arranged by size: 4090 763 384 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2062 3442 1353 Size: 709 1380 3423 Fragments arranged by size: 3423 1380 709 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2163 2169 2296 4099 4473 4619 Size: 1064 138 574 6 127 1803 374 146 Cuts at: 4619 387 Size: 1280 Fragments arranged by size: 1803 1280 1064 574 374 146 138 127 6 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2700 3575 4172 4959 5227 1239 Size: 393 1068 875 597 787 268 1524 Fragments arranged by size: 1524 1068 875 787 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2094 2094 Size: 5512 EcoO109I rG'GnC_Cy Cuts at: 2099 2282 2338 2099 Size: 183 56 5273 Fragments arranged by size: 5273 183 56 EcoRI G'AATT_C Cuts at: 2821 3199 2821 Size: 378 5134 FspI TGC'GCA Cuts at: 4018 4018 Size: 5512 HaeII r_GCGC'y Cuts at: 1678 4659 1678 Size: 2981 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2162 2194 4153 4185 4227 Size: 32 135 32 590 32 1959 32 42 Cuts at: 4227 4259 1373 Size: 32 2626 Fragments arranged by size: 2626 1959 590 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2404 2856 3102 669 Size: 988 281 466 452 246 3079 Fragments arranged by size: 3079 988 466 452 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5512 HpaI GTT'AAC Cuts at: 2404 3102 2404 Size: 698 4814 KpnI G_GTAC'C Cuts at: 2837 2837 Size: 5512 MfeI C'AATT_G Cuts at: 3111 3111 Size: 5512 MluI A'CGCG_T Cuts at: 5388 5388 Size: 5512 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2689 4626 4810 1305 Size: 1384 1937 184 2007 Fragments arranged by size: 2007 1937 1384 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3690 Fragments arranged by size: 3690 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3470 3829 3988 518 Size: 648 2304 359 159 2042 Fragments arranged by size: 2304 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2928 3605 4753 4998 1606 Size: 1322 677 1148 245 2120 Fragments arranged by size: 2120 1322 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4960 NdeI CA'TA_TG Cuts at: 387 387 Size: 5512 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5512 NotI GC'GGCC_GC Cuts at: 2196 2959 2196 Size: 763 4749 NsiI A_TGCA'T Cuts at: 2010 2010 Size: 5512 NspI r_CATG'y Cuts at: 1132 1229 2870 4415 1132 Size: 97 1641 1545 2229 Fragments arranged by size: 2229 1641 1545 97 PasI CC'CwG_GG Cuts at: 1348 2286 1348 Size: 938 4574 PciI A'CATG_T Cuts at: 4411 4411 Size: 5512 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5512 PmeI GTTT'AAAC Cuts at: 2816 2816 Size: 5512 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3581 3613 5094 5126 3581 Size: 32 1481 32 3967 Fragments arranged by size: 3967 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2099 2282 2338 2099 Size: 183 56 5273 Fragments arranged by size: 5273 183 56 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5512 PsiI TTA'TAA Cuts at: 3082 3082 Size: 5512 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2743 2775 2743 Size: 32 5480 PstI C_TGCA'G Cuts at: 830 2864 830 Size: 2034 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5512 SalI G'TCGA_C Cuts at: 1936 2854 1936 Size: 918 4594 SbfI CC_TGCA'GG Cuts at: 2864 2864 Size: 5512 ScaI AGT'ACT Cuts at: 3760 5472 3760 Size: 1712 3800 SfcI C'TryA_G Cuts at: 826 1045 2277 2649 2860 3995 4676 4867 826 Size: 219 1232 372 211 1135 681 191 1471 Fragments arranged by size: 1471 1232 1135 681 372 219 211 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2237 2237 Size: 5512 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5512 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5512 SmaI CCC'GGG Cuts at: 1872 2839 1872 Size: 967 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2327 3616 4517 4779 820 Size: 197 926 384 1289 901 262 1553 Fragments arranged by size: 1553 1289 926 901 384 262 197 SnaBI TAC'GTA Cuts at: 493 3303 493 Size: 2810 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5512 SphI G_CATG'C Cuts at: 1132 2870 1132 Size: 1738 3774 SspI AAT'ATT Cuts at: 5 52 3436 5 Size: 47 3384 2081 Fragments arranged by size: 3384 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5512 StyI C'CwwG_G Cuts at: 513 1065 2611 2951 513 Size: 552 1546 340 3074 Fragments arranged by size: 3074 1546 552 340 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2170 2461 3539 3556 3709 3894 4339 Size: 1255 590 291 1078 17 153 185 445 Cuts at: 4339 325 Size: 1498 Fragments arranged by size: 1498 1255 1078 590 445 291 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2408 3758 5470 Size: 274 80 33 51 1443 431 1350 1712 Cuts at: 5470 96 Size: 138 Fragments arranged by size: 1712 1443 1350 431 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5472 5504 5472 Size: 32 5480 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5512 XbaI T'CTAG_A Cuts at: 2848 2848 Size: 5512 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5512 XmaI C'CCGG_G Cuts at: 1870 2837 1870 Size: 967 4545 XmnI GAAnn'nnTTC Cuts at: 3641 3641 Size: 5512 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3320 276 Size: 53 83 186 936 1786 2468 Fragments arranged by size: 2468 1786 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI SalI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PspOMI PspXI PvuII RsrII SacI SacII SanDI SapI SexAI SfoI SrfI SwaI XcmI