################################## # NO CUT ENZYMES ( 159 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AvaI AviII AvrII BalI BamHI BbeI BbrPI BciVI BcnI BfrI BglI BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaWI BseAI BsiEI BsiHKAI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV FseI FspI HaeII HgaI Hin1I HincII HindII HindIII HpaI KasI KpnI KspI MaeII MaeIII MamI MfeI MluI MluNI MroI MscI MseI MunI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI SspBI SspI StuI SwaI TaqI Tru9I Tsp45I TthHB8I Van91I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### AccII 594 CGCG CG^CG AcsI 394 AAATTC [GA]^AATT[CT] ApoI 394 AAATTC [GA]^AATT[CT] AspI 324 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaII 323 GGACC G^G[AT]CC BanII 307 GAGCCC G[GA]GC[CT]^C BbsI 729 GAAGAC GAAGAC(2/6) BbvCI 148 GCTGAGG CCTCAGC(-5/-2) BcgI 647 CGATGCACTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 686 TGATCA T^GATCA BfaI 40 CTAG C^TAG BglII 162 AGATCT A^GATCT BpmI 587 CTGGAG CTGGAG(16/14) BpuAI 729 GAAGAC GAAGAC(2/6) BsaI 478 GAGACC GGTCTC(1/5) BsgI 666 CTGCAC GTGCAG(16/14) BsmAI 478 GAGAC GTCTC(1/5) BstUI 594 CGCG CG^CG BstYI 162 AGATCT [GA]^GATC[CT] CfoI 595 GCGC GCG^C Cfr13I 323 GGACC G^G[ACGT]CC DraII 322 GGGACCC [GA]G^G[ACGT]CC[CT] EaeI 66 TGGCCG [CT]^GGCC[GA] EarI 374 CTCTTC CTCTTC(1/4) EcoO109I 322 GGGACCC [GA]G^G[ACGT]CC[CT] EcoT14I 106 CCAAGG C^C[AT][AT]GG FauI 569 GCGGG CCCGC(4/6) FbaI 686 TGATCA T^GATCA HapII 427 CCGG C^CGG HhaI 595 GCGC GCG^C HinP1I 595 GCGC G^CGC HpaII 427 CCGG C^CGG Ksp632I 374 CTCTTC CTCTTC(1/4) MaeI 40 CTAG C^TAG MflI 162 AGATCT [GA]^GATC[CT] MspA1I 19 CAGCTG C[AC]G^C[GT]G MspI 427 CCGG C^CGG MvnI 594 CGCG CG^CG MwoI 159 GCCAGATCTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NspI 759 ACATGC [GA]CATG^[CT] PflFI 324 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC PleI 295 GACTC GAGTC(4/5) PpuMI 322 GGGACCC [GA]G^G[AT]CC[CT] PvuII 19 CAGCTG CAG^CTG Sau96I 323 GGACC G^G[ACGT]CC StyI 106 CCAAGG C^C[AT][AT]GG Tsp509I 395 AATT ^AATT Tth111I 324 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC XcmI 339 CCAGCCGATAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 162 AGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AlwNI 19 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG 252 CAGGTTCTG BbvI 597 GCAGC GCAGC(8/12) 21 GCTGC BmyI 307 GAGCCC G[AGT]GC[ACT]^C 493 GTGCCC Bsp1286I 307 GAGCCC G[AGT]GC[ACT]^C 493 GTGCCC BspMI 481 ACCTGC ACCTGC(4/8) 251 GCAGGT BsrI 327 CCAGT ACTGG(1/-1) 717 CCAGT Cac8I 630 GCCTGC GC[ACGT]^[ACGT]GC 763 GCTGGC DpnII 163 GATC ^GATC 687 GATC EcoT22I 1 ATGCAT ATGCA^T 280 ATGCAT HaeIII 67 GGCC GG^CC 214 GGCC MboI 163 GATC ^GATC 687 GATC NdeII 163 GATC ^GATC 687 GATC NsiI 1 ATGCAT ATGCA^T 280 ATGCAT PstI 203 CTGCAG CTGCA^G 483 CTGCAG Sau3AI 163 GATC ^GATC 687 GATC SbfI 202 CCTGCAGG CCTGCA^GG 482 CCTGCAGG SfcI 203 CTGCAG C^T[GA][CT]AG 483 CTGCAG Sse8387I 202 CCTGCAGG CCTGCA^GG 482 CCTGCAGG TseI 21 GCTGC G^C[AT]GC 597 GCAGC ######################################### # 3 OR MORE CUT ENZYMES ( 30 enzymes) # ######################################### AciI AfaI AluI BanI BsaJI BseRI BsiYI BslI BsmFI BssKI BstF5I BstNI DdeI EcoRII Fnu4HI FokI HinfI HphI ItaI MboII MnlI MslI MvaI NlaIII NlaIV RsaI ScrFI SfaNI TfiI TspRI