(Circular) (Six-base) MAPSORT of: pFN21AE3101.seq Check: 1556 from: 1 to: 5506 pFN21AE3101 5506 bp With 182 enzymes: SgfI * March 28, 2012 12:50 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3316 278 Size: 53 83 186 936 1780 2468 Fragments arranged by size: 2468 1780 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2849 1937 Size: 912 4594 Acc65I G'GTAC_C Cuts at: 2827 2827 Size: 5506 AclI AA'CG_TT Cuts at: 1563 3633 4006 1563 Size: 2070 373 3063 Fragments arranged by size: 3063 2070 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2488 3569 4953 5221 1632 Size: 856 1081 1384 268 1917 Fragments arranged by size: 1917 1384 1081 856 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5309 AflIII A'CryG_T Cuts at: 1203 4405 5382 1203 Size: 3202 977 1327 Fragments arranged by size: 3202 1327 977 AgeI A'CCGG_T Cuts at: 4337 4337 Size: 5506 AhdI GACnn_n'nnGTC Cuts at: 4235 4235 Size: 5506 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5506 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2513 1184 Size: 1329 4177 AlwNI CAG_nnn'CTG Cuts at: 2029 2262 3249 4821 2029 Size: 233 987 1572 2714 Fragments arranged by size: 2714 1572 987 233 ApaLI G'TGCA_C Cuts at: 3563 4719 3563 Size: 1156 4350 ApoI r'AATT_y Cuts at: 1493 2399 2815 3041 3193 5302 1493 Size: 906 416 226 152 2109 1697 Fragments arranged by size: 2109 1697 906 416 226 152 AseI AT'TA_AT Cuts at: 160 1032 4060 160 Size: 872 3028 1606 Fragments arranged by size: 3028 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5506 AvaI C'yCGr_G Cuts at: 1870 1943 2831 1870 Size: 73 888 4545 Fragments arranged by size: 4545 888 73 BamHI G'GATC_C Cuts at: 2836 3203 2836 Size: 367 5139 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2061 2198 2406 2827 4282 Size: 325 217 627 274 137 208 421 1455 Cuts at: 4282 618 Size: 1842 Fragments arranged by size: 1842 1455 627 421 325 274 217 208 137 BanII G_rGCy'C Cuts at: 1391 2316 2825 1391 Size: 925 509 4072 Fragments arranged by size: 4072 925 509 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2741 928 Size: 971 842 3693 Fragments arranged by size: 3693 971 842 BbvCI CC'TCA_GC Cuts at: 2154 2154 Size: 5506 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2641 2675 3697 3731 1903 Size: 34 50 34 620 34 1022 34 3678 Fragments arranged by size: 3678 1022 620 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3398 4608 3398 Size: 1210 4296 BclI T'GATC_A Cuts at: 1548 2691 1548 Size: 1143 4363 BfrBI ATG'CAT Cuts at: 2008 2287 2008 Size: 279 5227 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4117 136 Size: 107 122 71 631 605 2445 1525 Fragments arranged by size: 2445 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2167 5501 2167 Size: 3334 2172 BlpI GC'TnA_GC Cuts at: 2923 2923 Size: 5506 Bme1580I G_kGCm'C Cuts at: 1790 2502 3567 4723 1790 Size: 712 1065 1156 2573 Fragments arranged by size: 2573 1156 1065 712 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5506 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2326 3237 4195 Size: 416 53 83 399 24 909 911 958 Cuts at: 4195 442 Size: 1753 Fragments arranged by size: 1753 958 911 909 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5506 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2613 4166 1239 Size: 1374 1553 2579 Fragments arranged by size: 2579 1553 1374 Bpu10I CC'TnA_GC Cuts at: 2154 2154 Size: 5506 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3631 4496 4794 3631 Size: 865 298 4343 Fragments arranged by size: 4343 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2477 4169 882 Size: 617 978 1692 2219 Fragments arranged by size: 2219 1692 978 617 BsaAI yAC'GTr Cuts at: 493 1204 3297 493 Size: 711 2093 2702 Fragments arranged by size: 2702 2093 711 BsaBI GATnn'nnATC Cuts at: 5500 5500 Size: 5506 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3313 3695 4330 275 Size: 53 83 186 936 1780 382 635 1451 Fragments arranged by size: 1780 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3939 4337 4611 4758 5169 3939 Size: 398 274 147 411 4276 Fragments arranged by size: 4276 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2708 2738 1178 Size: 30 165 30 1305 30 3946 Fragments arranged by size: 3946 1305 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2003 2171 2367 2729 1186 Size: 817 168 196 362 3963 Fragments arranged by size: 3963 817 362 196 168 BseYI C'CCAG_C Cuts at: 4709 5205 4709 Size: 496 5010 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2656 1163 Size: 573 920 4013 Fragments arranged by size: 4013 920 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2956 3717 4745 1540 Size: 275 186 955 761 1028 2301 Fragments arranged by size: 2301 1028 955 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3567 3652 4723 3567 Size: 85 1071 4350 Fragments arranged by size: 4350 1071 85 BsmI GAATG_Cn' Cuts at: 3017 3110 3017 Size: 93 5413 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2316 2502 2825 3567 3652 4723 1391 Size: 399 526 186 323 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 526 399 323 186 85 BspEI T'CCGG_A Cuts at: 5169 5169 Size: 5506 BspHI T'CATG_A Cuts at: 3395 5125 3395 Size: 1730 3776 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2247 2495 2861 844 Size: 1403 248 366 3489 Fragments arranged by size: 3489 1403 366 248 BsrBI CCG'CTC Cuts at: 1394 1823 3393 1394 Size: 429 1570 3507 Fragments arranged by size: 3507 1570 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4001 4175 65 Size: 1183 2753 174 1396 Fragments arranged by size: 2753 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4150 4337 1700 Size: 110 2340 187 2869 Fragments arranged by size: 2869 2340 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5506 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5506 BssSI C'ACGA_G Cuts at: 3566 4578 3566 Size: 1012 4494 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5506 BstBI TT'CG_AA Cuts at: 4310 4310 Size: 5506 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5506 BstYI r'GATC_y Cuts at: 1972 2167 2836 3203 3588 3605 5057 5143 5155 Size: 195 669 367 385 17 1452 86 12 Cuts at: 5155 5501 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 669 385 367 346 195 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 4954 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4767 Fragments arranged by size: 4767 683 56 BtsI GCAGTG_nn' Cuts at: 2187 3022 3816 3836 2187 Size: 835 794 20 3857 Fragments arranged by size: 3857 835 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5506 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5471 DraI TTT'AAA Cuts at: 2810 2810 Size: 5506 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4513 809 Size: 507 3197 1802 Fragments arranged by size: 3197 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2071 2953 3842 Size: 54 1425 50 275 18 241 882 889 Cuts at: 3842 5471 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 882 275 241 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2953 1537 Size: 275 1141 4090 Fragments arranged by size: 4090 1141 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2385 3436 1353 Size: 1032 1051 3423 Fragments arranged by size: 3423 1051 1032 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4093 4467 4613 387 Size: 1064 138 2504 374 146 1280 Fragments arranged by size: 2504 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2488 2613 3569 4166 4953 5221 1239 Size: 393 856 125 956 597 787 268 1524 Fragments arranged by size: 1524 956 856 787 597 393 268 125 EcoO109I rG'GnC_Cy Cuts at: 2328 2328 Size: 5506 EcoRI G'AATT_C Cuts at: 2815 3193 2815 Size: 378 5128 FspI TGC'GCA Cuts at: 4012 4012 Size: 5506 HaeII r_GCGC'y Cuts at: 1678 4653 1678 Size: 2975 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4147 4179 4221 4253 1373 Size: 32 135 32 2575 32 42 32 2626 Fragments arranged by size: 2626 2575 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2850 3096 669 Size: 988 281 912 246 3079 Fragments arranged by size: 3079 988 912 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5506 HpaI GTT'AAC Cuts at: 3096 3096 Size: 5506 KpnI G_GTAC'C Cuts at: 2831 2831 Size: 5506 MfeI C'AATT_G Cuts at: 3105 3105 Size: 5506 MluI A'CGCG_T Cuts at: 5382 5382 Size: 5506 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4620 4804 1305 Size: 3315 184 2007 Fragments arranged by size: 3315 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3684 Fragments arranged by size: 3684 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2067 2651 2748 3464 3823 3982 518 Size: 648 901 584 97 716 359 159 2042 Fragments arranged by size: 2042 901 716 648 584 359 159 97 MspA1I CmG'CkG Cuts at: 1606 2026 2804 2922 3599 4747 4992 1606 Size: 420 778 118 677 1148 245 2120 Fragments arranged by size: 2120 1148 778 677 420 245 118 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4954 NdeI CA'TA_TG Cuts at: 387 387 Size: 5506 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5506 NotI GC'GGCC_GC Cuts at: 2953 2953 Size: 5506 NsiI A_TGCA'T Cuts at: 2010 2289 2010 Size: 279 5227 NspI r_CATG'y Cuts at: 1132 1229 2768 2864 4409 1132 Size: 97 1539 96 1545 2229 Fragments arranged by size: 2229 1545 1539 97 96 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5506 PciI A'CATG_T Cuts at: 4405 4405 Size: 5506 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5506 PfoI T'CCnGG_A Cuts at: 2590 2590 Size: 5506 PmeI GTTT'AAAC Cuts at: 2810 2810 Size: 5506 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3575 3607 5088 5120 3575 Size: 32 1481 32 3961 Fragments arranged by size: 3961 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2328 2328 Size: 5506 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5506 PsiI TTA'TAA Cuts at: 3076 3076 Size: 5506 PstI C_TGCA'G Cuts at: 830 2212 2492 2858 830 Size: 1382 280 366 3478 Fragments arranged by size: 3478 1382 366 280 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5506 PvuII CAG'CTG Cuts at: 2026 2804 2026 Size: 778 4728 SalI G'TCGA_C Cuts at: 1936 2848 1936 Size: 912 4594 SanDI GG'GwC_CC Cuts at: 2328 2328 Size: 5506 SbfI CC_TGCA'GG Cuts at: 2212 2492 2858 2212 Size: 280 366 4860 Fragments arranged by size: 4860 366 280 ScaI AGT'ACT Cuts at: 3754 5466 3754 Size: 1712 3794 SfcI C'TryA_G Cuts at: 826 1045 2208 2488 2854 3989 4670 4861 826 Size: 219 1163 280 366 1135 681 191 1471 Fragments arranged by size: 1471 1163 1135 681 366 280 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5506 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5506 SmaI CCC'GGG Cuts at: 1872 2833 1872 Size: 961 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3610 4511 4773 820 Size: 197 926 1667 901 262 1553 Fragments arranged by size: 1667 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3297 493 Size: 2804 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5506 SphI G_CATG'C Cuts at: 1132 2864 1132 Size: 1732 3774 SspI AAT'ATT Cuts at: 5 52 3430 5 Size: 47 3378 2081 Fragments arranged by size: 3378 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5506 StyI C'CwwG_G Cuts at: 513 1065 2111 2945 513 Size: 552 1046 834 3074 Fragments arranged by size: 3074 1046 834 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3533 3550 3703 3888 4333 325 Size: 1255 1953 17 153 185 445 1498 Fragments arranged by size: 1953 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2473 3752 5464 Size: 274 80 33 51 1443 496 1279 1712 Cuts at: 5464 96 Size: 138 Fragments arranged by size: 1712 1443 1279 496 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2708 2740 5466 5498 2708 Size: 32 2726 32 2716 Fragments arranged by size: 2726 2716 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2332 1316 Size: 1016 4490 XbaI T'CTAG_A Cuts at: 2842 2842 Size: 5506 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2351 2351 Size: 5506 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5506 XmaI C'CCGG_G Cuts at: 1870 2831 1870 Size: 961 4545 XmnI GAAnn'nnTTC Cuts at: 3635 3635 Size: 5506 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3314 276 Size: 53 83 186 936 1780 2468 Fragments arranged by size: 2468 1780 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SapI SexAI SfiI SfoI SrfI SwaI