################################## # NO CUT ENZYMES ( 117 enzymes) # ################################## AatII Acc65I AccII AccIII AflIII AgeI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspHI AspI AvaI AviII AvrII BalI BanII BbrPI BcgI BciVI BglII BlnI BlpI Bpu1102I BsaAI BseAI BsiEI BsiHKAI BsiWI BsoBI BspDI BspEI BspHI BspLU11I BsrBI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstZ17I Bsu36I CelII ClaI CpoI DraII DraIII DrdI EagI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I FseI FspI HindIII HpaI KpnI KspI MluI MluNI MroI MscI MspA1I MvnI NaeI NdeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI PvuI PvuII RcaI RsrII SacI SacII SalI SbfI ScaI SexAI SfiI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SwaI Tth111I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 58 enzymes) # ################################### AccI 667 GTAGAC GT^[AC][GT]AC AclI 1563 AACGTT AA^CGTT AcyI 1199 GGCGCC G[GA]^CG[CT]C AflII 1114 CTTAAG C^TTAAG BamHI 1896 GGATCC G^GATCC BanI 1199 GGCGCC G^G[CT][GA]CC BbeI 1199 GGCGCC GGCGC^C BbvCI 1582 GCTGAGG CCTCAGC(-5/-2) BclI 261 TGATCA T^GATCA BcnI 2298 CCGGG CC^[GC]GG BfaI 722 CTAG C^TAG BfrI 1114 CTTAAG C^TTAAG BglI 2147 GCCCAGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BmyI 56 GGGCAC G[AGT]GC[ACT]^C BsaHI 1199 GGCGCC G[GA]^CG[CT]C BsaI 1517 GAGACC GGTCTC(1/5) BsaWI 243 ACCGGA [AT]^CCGG[AT] BsgI 1847 GTGCAG GTGCAG(16/14) BsmBI 1503 GAGACG CGTCTC(1/5) BsmI 627 GAATGC GAATGC(1/-1) Bsp1286I 56 GGGCAC G[AGT]GC[ACT]^C Bsp1407I 2464 TGTACA T^GTACA BspMI 2119 ACCTGC ACCTGC(4/8) BsrFI 538 ACCGGC [GA]^CCGG[CT] BsrGI 2464 TGTACA T^GTACA BstAPI 1485 GCAGTCTATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 2436 CCAAGAGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 1896 GGATCC [GA]^GATC[CT] CfoI 1200 GCGC GCG^C Cfr10I 538 ACCGGC [GA]^CCGG[CT] EcoRV 25 GATATC GAT^ATC EcoT22I 760 ATGCAT ATGCA^T FauI 52 GCGGG CCCGC(4/6) FbaI 261 TGATCA T^GATCA HaeII 1199 GGCGCC [GA]GCGC^[CT] HhaI 1200 GCGC GCG^C Hin1I 1199 GGCGCC G[GA]^CG[CT]C HinP1I 1200 GCGC G^CGC HincII 1420 GTTGAC GT[CT]^[GA]AC HindII 1420 GTTGAC GT[CT]^[GA]AC KasI 1199 GGCGCC G^GCGCC MaeI 722 CTAG C^TAG MflI 1896 GGATCC [GA]^GATC[CT] NarI 1199 GGCGCC GG^CGCC NciI 2298 CCGGG CC^[GC]GG NsiI 760 ATGCAT ATGCA^T NspI 1767 GCATGC [GA]CATG^[CT] PflMI 64 CCAGGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Psp1406I 1563 AACGTT AA^CGTT SapI 1108 GAAGAGC GCTCTTC(1/4) SfoI 1199 GGCGCC GGC^GCC SmlI 1114 CTTAAG C^T[CT][GA]AG SphI 1767 GCATGC GCATG^C SspBI 2464 TGTACA T^GTACA SspI 218 AATATT AAT^ATT StuI 1916 AGGCCT AGG^CCT Van91I 64 CCAGGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 1896 GGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AhdI 688 GACGAGTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1505 GACGTAGGGTC AlwNI 828 CAGGGGCTG CAG[ACGT][ACGT][ACGT]^CTG 1477 CAGCGTCTG AspEI 688 GACGAGTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1505 GACGTAGGGTC BbvI 1849 GCAGC GCAGC(8/12) 2270 GCAGC BpmI 1019 CTCCAG CTGGAG(16/14) 2040 CTCCAG BsaBI 103 GATCACTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 1984 GATCCTAATC BseRI 1585 GAGGAG GAGGAG(10/8) 2045 GAGGAG BsiYI 64 CCAGGAAGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 2206 CCTCAGTGGGG BslI 64 CCAGGAAGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 2206 CCTCAGTGGGG BsrDI 786 CATTGC GCAATG(2/0) 905 GCAATG BsrI 1532 ACTGG ACTGG(1/-1) 344 CCAGT Cac8I 396 GCTGGC GC[ACGT]^[ACGT]GC 1767 GCATGC DraI 487 TTTAAA TTT^AAA 1635 TTTAAA DsaI 1522 CCATGG C^C[GA][CT]GG 2279 CCATGG EaeI 408 TGGCCG [CT]^GGCC[GA] 540 CGGCCA Eam1105I 688 GACGAGTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1505 GACGTAGGGTC HgaI 1479 GCGTC GACGC(5/10) 2273 GCGTC MamI 103 GATCACTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 1984 GATCCTAATC MfeI 923 CAATTG C^AATTG 2367 CAATTG MunI 923 CAATTG C^AATTG 2367 CAATTG NcoI 1522 CCATGG C^CATGG 2279 CCATGG PleI 2349 GACTC GAGTC(4/5) 294 GAGTC PstI 298 CTGCAG CTGCA^G 1483 CTGCAG TseI 1849 GCAGC G^C[AT]GC 2270 GCAGC Tsp45I 911 GTGAC ^GT[GC]AC 2362 GTGAC XcmI 1522 CCATGGGGAAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 2366 CCAATTGCTTTATGG ######################################### # 3 OR MORE CUT ENZYMES ( 57 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI ApoI AvaII BbsI BpuAI BsaJI BsmAI BsmFI BssKI BstF5I BstNI Cfr13I DdeI DpnII EarI EcoRI EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI Ksp632I MaeII MaeIII MboI MboII MnlI MseI MslI MspI MvaI MwoI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TaqI TfiI Tru9I Tsp509I TspRI TthHB8I