(Circular) (Six-base) MAPSORT of: pFN21AE1195.seq Check: 4983 from: 1 to: 7189 pFN21AE1195 7189 bp With 182 enzymes: SgfI * March 28, 2012 12:47 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4999 278 Size: 53 83 186 936 3463 2468 Fragments arranged by size: 3463 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2673 4532 1937 Size: 736 1859 4594 Fragments arranged by size: 4594 1859 736 Acc65I G'GTAC_C Cuts at: 4510 4510 Size: 7189 AclI AA'CG_TT Cuts at: 1563 3569 5316 5689 1563 Size: 2006 1747 373 3063 Fragments arranged by size: 3063 2006 1747 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2034 3335 3729 3934 4407 5252 6636 6904 Size: 402 1301 394 205 473 845 1384 268 Cuts at: 6904 1632 Size: 1917 Fragments arranged by size: 1917 1384 1301 845 473 402 394 268 205 AflII C'TTAA_G Cuts at: 820 1017 3119 820 Size: 197 2102 4890 Fragments arranged by size: 4890 2102 197 AflIII A'CryG_T Cuts at: 1203 6088 7065 1203 Size: 4885 977 1327 Fragments arranged by size: 4885 1327 977 AgeI A'CCGG_T Cuts at: 6020 6020 Size: 7189 AhdI GACnn_n'nnGTC Cuts at: 2698 3515 5918 2698 Size: 817 2403 3969 Fragments arranged by size: 3969 2403 817 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7189 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3600 1184 Size: 2416 4773 AlwNI CAG_nnn'CTG Cuts at: 2838 3487 4932 6504 2838 Size: 649 1445 1572 3523 Fragments arranged by size: 3523 1572 1445 649 ApaLI G'TGCA_C Cuts at: 5246 6402 5246 Size: 1156 6033 ApoI r'AATT_y Cuts at: 1493 2408 2430 2722 2786 2948 3039 3049 4181 Size: 915 22 292 64 162 91 10 1132 Cuts at: 4181 4391 4411 4498 4724 4876 6985 1493 Size: 210 20 87 226 152 2109 1697 Fragments arranged by size: 2109 1697 1132 915 292 226 210 162 152 91 87 64 22 20 10 AseI AT'TA_AT Cuts at: 160 1032 5743 160 Size: 872 4711 1606 Fragments arranged by size: 4711 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7189 AvaI C'yCGr_G Cuts at: 1870 1943 4514 1870 Size: 73 2571 4545 Fragments arranged by size: 4545 2571 73 BamHI G'GATC_C Cuts at: 3901 4519 4886 3901 Size: 618 367 6204 Fragments arranged by size: 6204 618 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3204 4510 5965 618 Size: 325 217 627 1417 1306 1455 1842 Fragments arranged by size: 1842 1455 1417 1306 627 325 217 BanII G_rGCy'C Cuts at: 1391 4508 1391 Size: 3117 4072 BbeI G_GCGC'C Cuts at: 3208 3208 Size: 7189 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2546 2694 2820 2834 4358 928 Size: 971 647 148 126 14 1524 3759 Fragments arranged by size: 3759 1524 971 647 148 126 14 BbvCI CC'TCA_GC Cuts at: 3588 3588 Size: 7189 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5380 5414 1903 Size: 34 3443 34 3678 Fragments arranged by size: 3678 3443 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5081 6291 5081 Size: 1210 5979 BclI T'GATC_A Cuts at: 1548 2266 1548 Size: 718 6471 BfrBI ATG'CAT Cuts at: 2767 2767 Size: 7189 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 4158 5800 Size: 107 122 71 631 605 337 2149 1642 Cuts at: 5800 136 Size: 1525 Fragments arranged by size: 2149 1642 1525 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 7184 7184 Size: 7189 BlpI GC'TnA_GC Cuts at: 4606 4606 Size: 7189 Bme1580I G_kGCm'C Cuts at: 1790 2065 5250 6406 1790 Size: 275 3185 1156 2573 Fragments arranged by size: 3185 2573 1156 275 BmgBI CAC'GTC Cuts at: 1318 2044 2615 1318 Size: 726 571 5892 Fragments arranged by size: 5892 726 571 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4920 5878 442 Size: 416 53 83 399 24 3503 958 1753 Fragments arranged by size: 3503 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7189 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3598 4371 3598 Size: 773 6416 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3009 4030 5849 1239 Size: 1770 1021 1819 2579 Fragments arranged by size: 2579 1819 1770 1021 Bpu10I CC'TnA_GC Cuts at: 3588 3588 Size: 7189 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5314 6179 6477 5314 Size: 865 298 6026 Fragments arranged by size: 6026 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3516 5852 882 Size: 617 2017 2336 2219 Fragments arranged by size: 2336 2219 2017 617 BsaAI yAC'GTr Cuts at: 493 1204 4980 493 Size: 711 3776 2702 Fragments arranged by size: 3776 2702 711 BsaBI GATnn'nnATC Cuts at: 2112 3993 7183 2112 Size: 1881 3190 2118 Fragments arranged by size: 3190 2118 1881 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3205 4996 5378 6013 Size: 53 83 186 936 1672 1791 382 635 Cuts at: 6013 275 Size: 1451 Fragments arranged by size: 1791 1672 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2248 5622 6020 6294 6441 6852 2248 Size: 3374 398 274 147 411 2585 Fragments arranged by size: 3374 2585 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 4250 4280 1178 Size: 30 165 30 2847 30 4087 Fragments arranged by size: 4087 2847 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3605 4065 1186 Size: 2419 460 4310 Fragments arranged by size: 4310 2419 460 BseYI C'CCAG_C Cuts at: 3480 6392 6888 3480 Size: 2912 496 3781 Fragments arranged by size: 3781 2912 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3873 1163 Size: 573 2137 4479 Fragments arranged by size: 4479 2137 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4639 5400 6428 1540 Size: 275 186 2638 761 1028 2301 Fragments arranged by size: 2638 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 5250 5335 6406 5250 Size: 85 1071 6033 Fragments arranged by size: 6033 1071 85 BsmI GAATG_Cn' Cuts at: 2638 4700 4793 2638 Size: 2062 93 5034 Fragments arranged by size: 5034 2062 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3502 3502 Size: 7189 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2065 4508 5250 5335 6406 1391 Size: 399 275 2443 742 85 1071 2174 Fragments arranged by size: 2443 2174 1071 742 399 275 85 BspEI T'CCGG_A Cuts at: 6852 6852 Size: 7189 BspHI T'CATG_A Cuts at: 5078 6808 5078 Size: 1730 5459 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4133 4544 844 Size: 3289 411 3489 Fragments arranged by size: 3489 3289 411 BsrBI CCG'CTC Cuts at: 1394 1823 5076 1394 Size: 429 3253 3507 Fragments arranged by size: 3507 3253 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2790 2917 5684 5858 65 Size: 1183 1542 127 2767 174 1396 Fragments arranged by size: 2767 1542 1396 1183 174 127 BsrFI r'CCGG_y Cuts at: 1700 1810 2543 5833 6020 1700 Size: 110 733 3290 187 2869 Fragments arranged by size: 3290 2869 733 187 110 BsrGI T'GTAC_A Cuts at: 96 4469 96 Size: 4373 2816 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7189 BssSI C'ACGA_G Cuts at: 5249 6261 5249 Size: 1012 6177 BstAPI GCAn_nnn'nTGC Cuts at: 794 3496 794 Size: 2702 4487 BstBI TT'CG_AA Cuts at: 5993 5993 Size: 7189 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7189 BstXI CCAn_nnnn'nTGG Cuts at: 4448 4448 Size: 7189 BstYI r'GATC_y Cuts at: 1972 3901 4519 4886 5271 5288 6740 6826 6838 Size: 1929 618 367 385 17 1452 86 12 Cuts at: 6838 7184 1972 Size: 346 1977 Fragments arranged by size: 1977 1929 1452 618 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 3527 4284 513 Size: 552 939 1523 757 3418 Fragments arranged by size: 3418 1523 939 757 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6450 Fragments arranged by size: 6450 683 56 BtsI GCAGTG_nn' Cuts at: 2737 4705 5499 5519 2737 Size: 1968 794 20 4407 Fragments arranged by size: 4407 1968 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7189 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7154 DraI TTT'AAA Cuts at: 2494 3642 4493 2494 Size: 1148 851 5190 Fragments arranged by size: 5190 1148 851 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6196 809 Size: 507 4880 1802 Fragments arranged by size: 4880 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2413 2545 4636 Size: 54 1425 50 275 18 583 132 2091 Cuts at: 4636 5525 7154 8 Size: 889 1629 43 Fragments arranged by size: 2091 1629 1425 889 583 275 132 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4636 1537 Size: 275 2824 4090 Fragments arranged by size: 4090 2824 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2010 2175 2291 2465 3108 3601 3606 4455 Size: 657 165 116 174 643 493 5 849 Cuts at: 4455 5119 1353 Size: 664 3423 Fragments arranged by size: 3423 849 664 657 643 493 174 165 116 5 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2036 5776 6150 6296 387 Size: 1064 138 447 3740 374 146 1280 Fragments arranged by size: 3740 1280 1064 447 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2034 3009 3335 3729 3934 4030 4407 Size: 393 402 975 326 394 205 96 377 Cuts at: 4407 5252 5849 6636 6904 1239 Size: 845 597 787 268 1524 Fragments arranged by size: 1524 975 845 787 597 402 394 393 377 326 268 205 96 EcoRI G'AATT_C Cuts at: 2722 2786 4391 4498 4876 2722 Size: 64 1605 107 378 5035 Fragments arranged by size: 5035 1605 378 107 64 EcoRV GAT'ATC Cuts at: 2032 2032 Size: 7189 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2279 3134 2279 Size: 855 6334 FspI TGC'GCA Cuts at: 5695 5695 Size: 7189 HaeII r_GCGC'y Cuts at: 1678 3208 6336 1678 Size: 1530 3128 2531 Fragments arranged by size: 3128 2531 1530 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2684 2716 2909 2941 3501 Size: 32 135 32 1112 32 193 32 560 Cuts at: 3501 3533 3893 3925 4066 4098 5830 5862 5904 Size: 32 360 32 141 32 1732 32 42 Cuts at: 5904 5936 1373 Size: 32 2626 Fragments arranged by size: 2626 1732 1112 560 360 193 141 135 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3427 4533 4779 669 Size: 988 281 1489 1106 246 3079 Fragments arranged by size: 3079 1489 1106 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7189 HpaI GTT'AAC Cuts at: 4779 4779 Size: 7189 KasI G'GCGC_C Cuts at: 3204 3204 Size: 7189 KpnI G_GTAC'C Cuts at: 4514 4514 Size: 7189 MfeI C'AATT_G Cuts at: 2928 4372 4788 2928 Size: 1444 416 5329 Fragments arranged by size: 5329 1444 416 MluI A'CGCG_T Cuts at: 7065 7065 Size: 7189 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2193 3706 6303 6487 1305 Size: 888 1513 2597 184 2007 Fragments arranged by size: 2597 2007 1513 888 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5367 Fragments arranged by size: 5367 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2599 3777 4070 5147 5506 5665 518 Size: 648 1433 1178 293 1077 359 159 2042 Fragments arranged by size: 2042 1433 1178 1077 648 359 293 159 MspA1I CmG'CkG Cuts at: 1606 4605 5282 6430 6675 1606 Size: 2999 677 1148 245 2120 Fragments arranged by size: 2999 2120 1148 677 245 NarI GG'CG_CC Cuts at: 3205 3205 Size: 7189 NcoI C'CATG_G Cuts at: 513 1065 2004 3527 4284 513 Size: 552 939 1523 757 3418 Fragments arranged by size: 3418 1523 939 757 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7189 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7189 NotI GC'GGCC_GC Cuts at: 4636 4636 Size: 7189 NsiI A_TGCA'T Cuts at: 2769 2769 Size: 7189 NspI r_CATG'y Cuts at: 1132 1229 3776 4547 6092 1132 Size: 97 2547 771 1545 2229 Fragments arranged by size: 2547 2229 1545 771 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7189 PciI A'CATG_T Cuts at: 6088 6088 Size: 7189 PflMI CCAn_nnn'nTGG Cuts at: 1108 2075 1108 Size: 967 6222 PmeI GTTT'AAAC Cuts at: 4493 4493 Size: 7189 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5258 5290 6771 6803 5258 Size: 32 1481 32 5644 Fragments arranged by size: 5644 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7189 PsiI TTA'TAA Cuts at: 4136 4431 4759 4136 Size: 295 328 6566 Fragments arranged by size: 6566 328 295 PstI C_TGCA'G Cuts at: 830 2307 3492 4541 830 Size: 1477 1185 1049 3478 Fragments arranged by size: 3478 1477 1185 1049 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7189 SalI G'TCGA_C Cuts at: 1936 4531 1936 Size: 2595 4594 SapI GCTCTTCn'nnn_ Cuts at: 3108 3108 Size: 7189 SbfI CC_TGCA'GG Cuts at: 4541 4541 Size: 7189 ScaI AGT'ACT Cuts at: 5437 7149 5437 Size: 1712 5477 SfcI C'TryA_G Cuts at: 826 1045 2303 3488 3887 4537 5672 6353 6544 Size: 219 1258 1185 399 650 1135 681 191 Cuts at: 6544 826 Size: 1471 Fragments arranged by size: 1471 1258 1185 1135 681 650 399 219 191 SfoI GGC'GCC Cuts at: 3206 3206 Size: 7189 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7189 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7189 SmaI CCC'GGG Cuts at: 1872 4516 1872 Size: 2644 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3119 5293 6194 6456 820 Size: 197 926 1176 2174 901 262 1553 Fragments arranged by size: 2174 1553 1176 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4980 493 Size: 4487 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7189 SphI G_CATG'C Cuts at: 1132 3776 4547 1132 Size: 2644 771 3774 Fragments arranged by size: 3774 2644 771 SspI AAT'ATT Cuts at: 5 52 2225 5113 5 Size: 47 2173 2888 2081 Fragments arranged by size: 2888 2173 2081 47 StuI AGG'CCT Cuts at: 1517 3923 1517 Size: 2406 4783 StyI C'CwwG_G Cuts at: 513 1065 2004 2153 2160 3527 3933 4284 4628 Size: 552 939 149 7 1367 406 351 344 Cuts at: 4628 513 Size: 3074 Fragments arranged by size: 3074 1367 939 552 406 351 344 149 7 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 5216 5233 5386 5571 6016 325 Size: 1255 3636 17 153 185 445 1498 Fragments arranged by size: 3636 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3878 4189 4469 Size: 274 80 33 51 1443 1901 311 280 Cuts at: 4469 5435 7147 96 Size: 966 1712 138 Fragments arranged by size: 1901 1712 1443 966 311 280 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7149 7181 7149 Size: 32 7157 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7189 XbaI T'CTAG_A Cuts at: 4525 4525 Size: 7189 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3534 4378 3534 Size: 844 6345 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7189 XmaI C'CCGG_G Cuts at: 1870 4514 1870 Size: 2644 4545 XmnI GAAnn'nnTTC Cuts at: 5318 5318 Size: 7189 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4997 276 Size: 53 83 186 936 3463 2468 Fragments arranged by size: 3463 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I FseI FspAI NaeI NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI SfiI SrfI SwaI