Restriction map for
pFN21AE1195
(Linear) (Six-base) MAPSORT of: KIEE1195.seq Check: 2591 from: 1 to: 2484
KIEE1195.orf
With 182 enzymes: SgfI *
March 22, 2012 15:50 ..
AccI GT'mk_AC
Cuts at: 0 668 2484
Size: 668 1816
AclI AA'CG_TT
Cuts at: 0 1564 2484
Size: 1564 920
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 29 1330 1724 1929 2402 2484
Size: 29 1301 394 205 473 82
Fragments arranged by size:
1301 473 394 205 82 29
AflII C'TTAA_G
Cuts at: 0 1114 2484
Size: 1114 1370
AhdI GACnn_n'nnGTC
Cuts at: 0 693 1510 2484
Size: 693 817 974
Fragments arranged by size:
974 817 693
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1595 2484
Size: 1595 889
AlwNI CAG_nnn'CTG
Cuts at: 0 833 1482 2484
Size: 833 649 1002
Fragments arranged by size:
1002 833 649
ApoI r'AATT_y
Cuts at: 0 403 425 717 781 943 1034 1044 2176
Size: 403 22 292 64 162 91 10 1132
Cuts at: 2176 2386 2406 2484
Size: 210 20 78
Fragments arranged by size:
1132 403 292 210 162 91 78 64
22 20 10
BamHI G'GATC_C
Cuts at: 0 1896 2484
Size: 1896 588
BanI G'GyrC_C
Cuts at: 0 1199 2484
Size: 1199 1285
BbeI G_GCGC'C
Cuts at: 0 1203 2484
Size: 1203 1281
BbsI GAAGACnn'nnnn_
Cuts at: 0 541 689 815 829 2353 2484
Size: 541 148 126 14 1524 131
Fragments arranged by size:
1524 541 148 131 126 14
BbvCI CC'TCA_GC
Cuts at: 0 1583 2484
Size: 1583 901
BclI T'GATC_A
Cuts at: 0 261 2484
Size: 261 2223
BfrBI ATG'CAT
Cuts at: 0 762 2484
Size: 762 1722
BglI GCCn_nnn'nGGC
Cuts at: 0 2153 2484
Size: 2153 331
Bme1580I G_kGCm'C
Cuts at: 0 60 2484
Size: 60 2424
BmgBI CAC'GTC
Cuts at: 0 39 610 2484
Size: 39 571 1874
Fragments arranged by size:
1874 571 39
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1593 2366 2484
Size: 1593 773 118
Fragments arranged by size:
1593 773 118
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1004 2025 2484
Size: 1004 1021 459
Fragments arranged by size:
1021 1004 459
Bpu10I CC'TnA_GC
Cuts at: 0 1583 2484
Size: 1583 901
BsaI GGTCTCn'nnnn_
Cuts at: 0 1511 2484
Size: 1511 973
BsaBI GATnn'nnATC
Cuts at: 0 107 1988 2484
Size: 107 1881 496
Fragments arranged by size:
1881 496 107
BsaHI Gr'CG_yC
Cuts at: 0 1200 2484
Size: 1200 1284
BsaWI w'CCGG_w
Cuts at: 0 243 2484
Size: 243 2241
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2245 2275 2484
Size: 2245 30 209
Fragments arranged by size:
2245 209 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1600 2060 2484
Size: 1600 460 424
Fragments arranged by size:
1600 460 424
BseYI C'CCAG_C
Cuts at: 0 1475 2484
Size: 1475 1009
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1868 2484
Size: 1868 616
BsmI GAATG_Cn'
Cuts at: 0 633 2484
Size: 633 1851
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1497 2484
Size: 1497 987
Bsp1286I G_dGCh'C
Cuts at: 0 60 2484
Size: 60 2424
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2128 2484
Size: 2128 356
BsrDI GCAATG_nn'
Cuts at: 0 785 912 2484
Size: 785 127 1572
Fragments arranged by size:
1572 785 127
BsrFI r'CCGG_y
Cuts at: 0 538 2484
Size: 538 1946
BsrGI T'GTAC_A
Cuts at: 0 2464 2484
Size: 2464 20
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1491 2484
Size: 1491 993
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2443 2484
Size: 2443 41
BstYI r'GATC_y
Cuts at: 0 1896 2484
Size: 1896 588
BtgI C'CryG_G
Cuts at: 0 1522 2279 2484
Size: 1522 757 205
Fragments arranged by size:
1522 757 205
BtsI GCAGTG_nn'
Cuts at: 0 732 2484
Size: 732 1752
DraI TTT'AAA
Cuts at: 0 489 1637 2484
Size: 489 1148 847
Fragments arranged by size:
1148 847 489
EaeI y'GGCC_r
Cuts at: 0 408 540 2484
Size: 408 132 1944
Fragments arranged by size:
1944 408 132
EarI CTCTTCn'nnn_
Cuts at: 0 5 170 286 460 1103 1596 1601 2450
Size: 5 165 116 174 643 493 5 849
Cuts at: 2450 2484
Size: 34
Fragments arranged by size:
849 643 493 174 165 116 34 5
5
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 31 2484
Size: 31 2453
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 29 1004 1330 1724 1929 2025 2402 2484
Size: 29 975 326 394 205 96 377 82
Fragments arranged by size:
975 394 377 326 205 96 82 29
EcoRI G'AATT_C
Cuts at: 0 717 781 2386 2484
Size: 717 64 1605 98
Fragments arranged by size:
1605 717 98 64
EcoRV GAT'ATC
Cuts at: 0 27 2484
Size: 27 2457
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 274 1129 2484
Size: 274 855 1355
Fragments arranged by size:
1355 855 274
HaeII r_GCGC'y
Cuts at: 0 1203 2484
Size: 1203 1281
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 679 711 904 936 1496 1528 1888 1920
Size: 679 32 193 32 560 32 360 32
Cuts at: 1920 2061 2093 2484
Size: 141 32 391
Fragments arranged by size:
679 560 391 360 193 141 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 1422 2484
Size: 1422 1062
KasI G'GCGC_C
Cuts at: 0 1199 2484
Size: 1199 1285
MfeI C'AATT_G
Cuts at: 0 923 2367 2484
Size: 923 1444 117
Fragments arranged by size:
1444 923 117
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 188 1701 2484
Size: 188 1513 783
Fragments arranged by size:
1513 783 188
MslI CAynn'nnrTG
Cuts at: 0 594 1772 2065 2484
Size: 594 1178 293 419
Fragments arranged by size:
1178 594 419 293
NarI GG'CG_CC
Cuts at: 0 1200 2484
Size: 1200 1284
NcoI C'CATG_G
Cuts at: 0 1522 2279 2484
Size: 1522 757 205
Fragments arranged by size:
1522 757 205
NsiI A_TGCA'T
Cuts at: 0 764 2484
Size: 764 1720
NspI r_CATG'y
Cuts at: 0 1771 2484
Size: 1771 713
PflMI CCAn_nnn'nTGG
Cuts at: 0 70 2484
Size: 70 2414
PsiI TTA'TAA
Cuts at: 0 2131 2426 2484
Size: 2131 295 58
Fragments arranged by size:
2131 295 58
PstI C_TGCA'G
Cuts at: 0 302 1487 2484
Size: 302 1185 997
Fragments arranged by size:
1185 997 302
SapI GCTCTTCn'nnn_
Cuts at: 0 1103 2484
Size: 1103 1381
SfcI C'TryA_G
Cuts at: 0 298 1483 1882 2484
Size: 298 1185 399 602
Fragments arranged by size:
1185 602 399 298
SfoI GGC'GCC
Cuts at: 0 1201 2484
Size: 1201 1283
SmlI C'TyrA_G
Cuts at: 0 1114 2484
Size: 1114 1370
SphI G_CATG'C
Cuts at: 0 1771 2484
Size: 1771 713
SspI AAT'ATT
Cuts at: 0 220 2484
Size: 220 2264
StuI AGG'CCT
Cuts at: 0 1918 2484
Size: 1918 566
StyI C'CwwG_G
Cuts at: 0 148 155 1522 1928 2279 2484
Size: 148 7 1367 406 351 205
Fragments arranged by size:
1367 406 351 205 148 7
TatI w'GTAC_w
Cuts at: 0 1873 2184 2464 2484
Size: 1873 311 280 20
Fragments arranged by size:
1873 311 280 20
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1529 2373 2484
Size: 1529 844 111
Fragments arranged by size:
1529 844 111
Enzymes that do cut:
AccI AclI AcuI AflII AhdI AlfI AlwNI ApoI
BamHI BanI BbeI BbsI BbvCI BclI BfrBI BglI
Bme1580I BmgBI BplI BpmI Bpu10I BsaI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsmI BsmBI Bsp1286I
BspMI BsrDI BsrFI BsrGI BstAPI BstXI BstYI BtgI
BtsI DraI EaeI EarI EciI Eco57MI EcoRI EcoRV
FalI HaeII Hin4I HincII KasI MfeI MmeI MslI
NarI NcoI NsiI NspI PflMI PsiI PstI SapI
SfcI SfoI SmlI SphI SspI StuI StyI TatI
XcmI
Enzymes that do not cut:
AarI AatII Acc65I AfeI AflIII AgeI AleI AloI
ApaI ApaLI AscI AseI AsiSI AvaI AvrII BaeI
BanII BcgI BciVI BglII BlpI BmrI BmtI BpuEI
BsaAI BsiEI BsiHKAI BsiWI BspEI BspHI BsrBI BssHII
BssSI BstBI BstEII BstZ17I Bsu36I BtgZI ClaI CspCI
DraIII DrdI EagI EcoICRI EcoNI EcoO109I FseI FspI
FspAI HindIII HpaI KpnI MluI MscI MspA1I NaeI
NdeI NgoMIV NheI NotI NruI PacI PasI PciI
PfoI PmeI PmlI PpiI PpuMI PshAI PspOMI PspXI
PsrI PvuI PvuII RsrII SacI SacII SalI SanDI
SbfI ScaI SexAI SfiI SgfI SgrAI SmaI SnaBI
SpeI SrfI SwaI TaqII TstI Tth111I XbaI XhoI
XmaI XmnI ZraI