Restriction map for
pFN21AE3435
##################################
# NO CUT ENZYMES ( 96 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AhdI Aor51HI ApaI AscI Asp718I
AspEI AspI AvaI AviII AvrII
BamHI BanII BbrPI BbvCI BcgI
BglI BlnI BlpI Bpu1102I BsaAI
BseAI BsiWI BsmBI BsoBI BspDI
BspEI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstZ17I CelII ClaI
CpoI DraI DraIII DrdI Eam1105I
Eco47III EcoNI EcoO65I FseI FspI
HincII HindII HindIII HpaI KpnI
KspI MluI MroI NaeI NgoMIV
NheI NruI NspV PacI PaeR7I
PflFI PflMI PmaCI PmeI PmlI
PshAI Psp1406I PvuI PvuII RsrII
SacI SacII SalI SapI SbfI
SexAI SfiI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SwaI
Tth111I Van91I XbaI XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 58 enzymes) #
###################################
AcyI 361 GGCGCC G[GA]^CG[CT]C
AflII 2301 CTTAAG C^TTAAG
AgeI 2396 ACCGGT A^CCGGT
AocI 202 CCTCAGG CC^T[ACGT]AGG
AseI 661 ATTAAT AT^TAAT
AsnI 661 ATTAAT AT^TAAT
Asp700I 484 GAAGTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII 271 GGTCC G^G[AT]CC
BalI 1773 TGGCCA TGG^CCA
BanI 361 GGCGCC G^G[CT][GA]CC
BbeI 361 GGCGCC GGCGC^C
BciVI 1901 GGATAC GTATCC(6/5)
BcnI 54 CCCGG CC^[GC]GG
BfrI 2301 CTTAAG C^TTAAG
BglII 432 AGATCT A^GATCT
BsaBI 2149 GATAGTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 361 GGCGCC G[GA]^CG[CT]C
BseRI 1646 GAGGAG GAGGAG(10/8)
BsiEI 46 CGGCCG CG[GA][CT]^CG
BsmI 2256 GAATGC GAATGC(1/-1)
BspHI 1767 TCATGA T^CATGA
BsrFI 2396 ACCGGT [GA]^CCGG[CT]
Bsu36I 202 CCTCAGG CC^T[ACGT]AGG
Cfr10I 2396 ACCGGT [GA]^CCGG[CT]
Cfr13I 271 GGTCC G^G[ACGT]CC
DraII 270 AGGTCCT [GA]G^G[ACGT]CC[CT]
DsaI 1494 CCATGG C^C[GA][CT]GG
EagI 46 CGGCCG C^GGCCG
EclXI 46 CGGCCG C^GGCCG
Eco52I 46 CGGCCG C^GGCCG
Eco81I 202 CCTCAGG CC^T[ACGT]AGG
EcoO109I 270 AGGTCCT [GA]G^G[ACGT]CC[CT]
EcoRV 1969 GATATC GAT^ATC
HaeII 361 GGCGCC [GA]GCGC^[CT]
HgaI 2030 GACGC GACGC(5/10)
Hin1I 361 GGCGCC G[GA]^CG[CT]C
KasI 361 GGCGCC G^GCGCC
MamI 2149 GATAGTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1745 CAATTG C^AATTG
MluNI 1773 TGGCCA TGG^CCA
MscI 1773 TGGCCA TGG^CCA
MspA1I 196 CCGCTG C[AC]G^C[GT]G
MunI 1745 CAATTG C^AATTG
NarI 361 GGCGCC GG^CGCC
NciI 54 CCCGG CC^[GC]GG
NcoI 1494 CCATGG C^CATGG
NlaIV 361 GGCGCC GG[ACGT]^[ACGT]CC
NotI 45 GCGGCCGC GC^GGCCGC
PinAI 2396 ACCGGT A^CCGGT
PpuMI 270 AGGTCCT [GA]G^G[AT]CC[CT]
PshBI 661 ATTAAT AT^TAAT
RcaI 1767 TCATGA T^CATGA
Sau96I 271 GGTCC G^G[ACGT]CC
ScaI 1884 AGTACT AGT^ACT
SfoI 361 GGCGCC GGC^GCC
StuI 1674 AGGCCT AGG^CCT
XcmI 1494 CCATGGGCAGACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 484 GAAGTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 38 enzymes) #
###################################
AccII 44 CGCG CG^CG
50 CGCG
AflIII 729 ACATGT A^C[GA][CT]GT
1144 ACATGT
Alw44I 278 GTGCAC G^TGCAC
725 GTGCAC
AlwI 574 GATCC GGATC(4/5)
522 GGATC
ApaLI 278 GTGCAC G^TGCAC
725 GTGCAC
AspHI 278 GTGCAC G[AT]GC[AT]^C
725 GTGCAC
BbsI 1960 GAAGAC GAAGAC(2/6)
2098 GAAGAC
BclI 1122 TGATCA T^GATCA
1386 TGATCA
BmyI 278 GTGCAC G[AGT]GC[ACT]^C
725 GTGCAC
BpuAI 1960 GAAGAC GAAGAC(2/6)
2098 GAAGAC
BsaI 27 GAGACC GGTCTC(1/5)
2227 GGTCTC
BsiHKAI 278 GTGCAC G[AT]GC[AT]^C
725 GTGCAC
BsmAI 27 GAGAC GTCTC(1/5)
2228 GTCTC
Bsp1286I 278 GTGCAC G[AGT]GC[ACT]^C
725 GTGCAC
Bsp1407I 1147 TGTACA T^GTACA
2695 TGTACA
BspLU11I 729 ACATGT A^CATGT
1144 ACATGT
BsrBI 208 GAGCGG CCGCTC(-3/-3)
226 GAGCGG
BsrGI 1147 TGTACA T^GTACA
2695 TGTACA
BstAPI 400 GCAAAACGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
442 GCAGCACCTGC
BstUI 44 CGCG CG^CG
50 CGCG
BstYI 432 AGATCT [GA]^GATC[CT]
522 GGATCT
CfoI 51 GCGC GCG^C
362 GCGC
EaeI 46 CGGCCG [CT]^GGCC[GA]
1773 TGGCCA
EcoRI 1718 GAATTC G^AATTC
2063 GAATTC
EcoT14I 825 CCTTGG C^C[AT][AT]GG
1494 CCATGG
FauI 81 CCCGC CCCGC(4/6)
228 GCGGG
FbaI 1122 TGATCA T^GATCA
1386 TGATCA
HhaI 51 GCGC GCG^C
362 GCGC
HinP1I 51 GCGC G^CGC
362 GCGC
MflI 432 AGATCT [GA]^GATC[CT]
522 GGATCT
MvnI 44 CGCG CG^CG
50 CGCG
NdeI 1981 CATATG CA^TATG
2589 CATATG
PstI 245 CTGCAG CTGCA^G
1160 CTGCAG
SmlI 2261 CTCAAG C^T[CT][GA]AG
2301 CTTAAG
SspBI 1147 TGTACA T^GTACA
2695 TGTACA
SspI 2109 AATATT AAT^ATT
2316 AATATT
StyI 825 CCTTGG C^C[AT][AT]GG
1494 CCATGG
XhoII 432 AGATCT [GA]^GATC[CT]
522 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 66 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwNI
ApoI BbvI BfaI BpmI BsaJI
BsaWI BsgI BsiYI BslI BsmFI
BspMI BsrDI BsrI BssKI BstF5I
BstNI BstXI Cac8I DdeI DpnII
EarI EcoRII EcoT22I Fnu4HI FokI
HaeIII HapII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MnlI MseI MslI
MspI MvaI MwoI NdeII NlaIII
NsiI NspI PleI RsaI Sau3AI
ScrFI SfaNI SfcI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I