(Circular) (Six-base) MAPSORT of: pFN21AE3435.seq Check: 7574 from: 1 to: 7486 pFN21AE3435 7486 bp With 182 enzymes: SgfI * March 28, 2012 12:45 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2461 3572 4262 2461 Size: 1111 690 5685 Fragments arranged by size: 5685 1111 690 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5296 278 Size: 53 83 186 936 3760 2468 Fragments arranged by size: 3760 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4829 1937 Size: 2892 4594 Acc65I G'GTAC_C Cuts at: 4807 4807 Size: 7486 AclI AA'CG_TT Cuts at: 1563 4785 5613 5986 1563 Size: 3222 828 373 3063 Fragments arranged by size: 3222 3063 828 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2453 2545 3033 5549 6933 7201 1632 Size: 821 92 488 2516 1384 268 1917 Fragments arranged by size: 2516 1917 1384 821 488 268 92 AflII C'TTAA_G Cuts at: 820 1017 4306 820 Size: 197 3289 4000 Fragments arranged by size: 4000 3289 197 AflIII A'CryG_T Cuts at: 1203 2734 3149 6385 7362 1203 Size: 1531 415 3236 977 1327 Fragments arranged by size: 3236 1531 1327 977 415 AgeI A'CCGG_T Cuts at: 4401 6317 4401 Size: 1916 5570 AhdI GACnn_n'nnGTC Cuts at: 6215 6215 Size: 7486 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7486 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3106 1184 Size: 1922 5564 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2326 2358 2326 Size: 32 7454 AlwNI CAG_nnn'CTG Cuts at: 2279 2453 2942 3225 5229 6801 2279 Size: 174 489 283 2004 1572 2964 Fragments arranged by size: 2964 2004 1572 489 283 174 ApaLI G'TGCA_C Cuts at: 2283 2730 5543 6699 2283 Size: 447 2813 1156 3070 Fragments arranged by size: 3070 2813 1156 447 ApoI r'AATT_y Cuts at: 1493 2654 3285 3492 3625 3723 3768 4068 4795 Size: 1161 631 207 133 98 45 300 727 Cuts at: 4795 5021 5173 7282 1493 Size: 226 152 2109 1697 Fragments arranged by size: 2109 1697 1161 727 631 300 226 207 152 133 98 45 AseI AT'TA_AT Cuts at: 160 1032 2667 6040 160 Size: 872 1635 3373 1606 Fragments arranged by size: 3373 1635 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7486 AvaI C'yCGr_G Cuts at: 1870 1943 4811 1870 Size: 73 2868 4545 Fragments arranged by size: 4545 2868 73 BamHI G'GATC_C Cuts at: 4816 5183 4816 Size: 367 7119 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2366 4807 6262 618 Size: 325 217 627 579 2441 1455 1842 Fragments arranged by size: 2441 1842 1455 627 579 325 217 BanII G_rGCy'C Cuts at: 1391 4805 1391 Size: 3414 4072 BbeI G_GCGC'C Cuts at: 2370 2370 Size: 7486 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3972 4110 928 Size: 971 2073 138 4304 Fragments arranged by size: 4304 2073 971 138 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5677 5711 1903 Size: 34 3740 34 3678 Fragments arranged by size: 3740 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3900 5378 6588 3900 Size: 1478 1210 4798 Fragments arranged by size: 4798 1478 1210 BclI T'GATC_A Cuts at: 1548 3127 3391 1548 Size: 1579 264 5643 Fragments arranged by size: 5643 1579 264 BfrBI ATG'CAT Cuts at: 2638 3713 4546 2638 Size: 1075 833 5578 Fragments arranged by size: 5578 1075 833 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6097 136 Size: 107 122 71 631 605 4425 1525 Fragments arranged by size: 4425 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2437 7481 2437 Size: 5044 2442 BlpI GC'TnA_GC Cuts at: 4903 4903 Size: 7486 Bme1580I G_kGCm'C Cuts at: 1790 2287 2734 5547 6703 1790 Size: 497 447 2813 1156 2573 Fragments arranged by size: 2813 2573 1156 497 447 BmgBI CAC'GTC Cuts at: 1318 3368 1318 Size: 2050 5436 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 5217 6175 442 Size: 416 53 83 399 24 3800 958 1753 Fragments arranged by size: 3800 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7486 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2386 2386 Size: 7486 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3531 3870 3879 4155 6146 1239 Size: 2292 339 9 276 1991 2579 Fragments arranged by size: 2579 2292 1991 339 276 9 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4251 5611 6476 6774 4251 Size: 1360 865 298 4963 Fragments arranged by size: 4963 1360 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2026 4238 6149 882 Size: 617 527 2212 1911 2219 Fragments arranged by size: 2219 2212 1911 617 527 BsaAI yAC'GTr Cuts at: 493 1204 5277 493 Size: 711 4073 2702 Fragments arranged by size: 4073 2702 711 BsaBI GATnn'nnATC Cuts at: 4158 7480 4158 Size: 3322 4164 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2367 5293 5675 6310 Size: 53 83 186 936 834 2926 382 635 Cuts at: 6310 275 Size: 1451 Fragments arranged by size: 2926 1451 936 834 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2028 3903 4401 5919 6317 6591 6738 7149 2028 Size: 1875 498 1518 398 274 147 411 2365 Fragments arranged by size: 2365 1875 1518 498 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3852 3882 1178 Size: 30 165 30 2449 30 4782 Fragments arranged by size: 4782 2449 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3666 1186 Size: 2480 5006 BseYI C'CCAG_C Cuts at: 2112 2127 3305 6689 7185 2112 Size: 15 1178 3384 496 2413 Fragments arranged by size: 3384 2413 1178 496 15 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2084 2955 3051 1163 Size: 573 348 871 96 5598 Fragments arranged by size: 5598 871 573 348 96 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2054 4936 5697 6725 1540 Size: 275 186 53 2882 761 1028 2301 Fragments arranged by size: 2882 2301 1028 761 275 186 53 BsiHKAI G_wGCw'C Cuts at: 2287 2734 5547 5632 6703 2287 Size: 447 2813 85 1071 3070 Fragments arranged by size: 3070 2813 1071 447 85 BsmI GAATG_Cn' Cuts at: 4267 4997 5090 4267 Size: 730 93 6663 Fragments arranged by size: 6663 730 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2287 2734 4805 5547 5632 6703 1391 Size: 399 497 447 2071 742 85 1071 2174 Fragments arranged by size: 2174 2071 1071 742 497 447 399 85 BspEI T'CCGG_A Cuts at: 7149 7149 Size: 7486 BspHI T'CATG_A Cuts at: 3772 5375 7105 3772 Size: 1603 1730 4153 Fragments arranged by size: 4153 1730 1603 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2461 3572 3913 4262 4331 4841 844 Size: 1617 1111 341 349 69 510 3489 Fragments arranged by size: 3489 1617 1111 510 349 341 69 BsrBI CCG'CTC Cuts at: 1394 1823 2215 2233 5373 1394 Size: 429 392 18 3140 3507 Fragments arranged by size: 3507 3140 429 392 18 BsrDI GCAATG_nn' Cuts at: 65 1248 3715 4134 4772 5981 6155 65 Size: 1183 2467 419 638 1209 174 1396 Fragments arranged by size: 2467 1396 1209 1183 638 419 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4401 6130 6317 1700 Size: 110 2591 1729 187 2869 Fragments arranged by size: 2869 2591 1729 187 110 BsrGI T'GTAC_A Cuts at: 96 3152 4700 96 Size: 3056 1548 2882 Fragments arranged by size: 3056 2882 1548 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7486 BssSI C'ACGA_G Cuts at: 5546 6558 5546 Size: 1012 6474 BstAPI GCAn_nnn'nTGC Cuts at: 794 2411 2453 794 Size: 1617 42 5827 Fragments arranged by size: 5827 1617 42 BstBI TT'CG_AA Cuts at: 6290 6290 Size: 7486 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7486 BstXI CCAn_nnnn'nTGG Cuts at: 2589 2697 3788 2589 Size: 108 1091 6287 Fragments arranged by size: 6287 1091 108 BstYI r'GATC_y Cuts at: 1972 2437 2527 4816 5183 5568 5585 7037 7123 Size: 465 90 2289 367 385 17 1452 86 Cuts at: 7123 7135 7481 1972 Size: 12 346 1977 Fragments arranged by size: 2289 1977 1452 465 385 367 346 90 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2208 2208 Size: 7486 BtgI C'CryG_G Cuts at: 513 1065 3499 513 Size: 552 2434 4500 Fragments arranged by size: 4500 2434 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6747 Fragments arranged by size: 6747 683 56 BtsI GCAGTG_nn' Cuts at: 3088 5002 5796 5816 3088 Size: 1914 794 20 4758 Fragments arranged by size: 4758 1914 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7486 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7451 DraI TTT'AAA Cuts at: 4790 4790 Size: 7486 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6493 809 Size: 507 5177 1802 Fragments arranged by size: 5177 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2051 3778 4933 Size: 54 1425 50 275 18 221 1727 1155 Cuts at: 4933 5822 7451 8 Size: 889 1629 43 Fragments arranged by size: 1727 1629 1425 1155 889 275 221 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2051 4933 1537 Size: 275 239 2882 4090 Fragments arranged by size: 4090 2882 275 239 EarI CTCTTCn'nnn_ Cuts at: 1353 2670 2785 3532 3822 4189 5416 1353 Size: 1317 115 747 290 367 1227 3423 Fragments arranged by size: 3423 1317 1227 747 367 290 115 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2130 2166 2187 6073 6447 6593 Size: 1064 138 541 36 21 3886 374 146 Cuts at: 6593 387 Size: 1280 Fragments arranged by size: 3886 1280 1064 541 374 146 138 36 21 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2453 2545 3033 3531 3870 3879 4155 Size: 393 821 92 488 498 339 9 276 Cuts at: 4155 5549 6146 6933 7201 1239 Size: 1394 597 787 268 1524 Fragments arranged by size: 1524 1394 821 787 597 498 488 393 339 276 268 92 9 EcoO109I rG'GnC_Cy Cuts at: 2276 2276 Size: 7486 EcoRI G'AATT_C Cuts at: 3723 4068 4795 5173 3723 Size: 345 727 378 6036 Fragments arranged by size: 6036 727 378 345 EcoRV GAT'ATC Cuts at: 3976 3976 Size: 7486 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2846 2846 Size: 7486 FspI TGC'GCA Cuts at: 5992 5992 Size: 7486 HaeII r_GCGC'y Cuts at: 1678 2370 6633 1678 Size: 692 4263 2531 Fragments arranged by size: 4263 2531 692 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3047 3079 3645 3677 6127 Size: 32 135 32 1475 32 566 32 2450 Cuts at: 6127 6159 6201 6233 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2450 1475 566 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4830 5076 669 Size: 988 281 2892 246 3079 Fragments arranged by size: 3079 2892 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7486 HpaI GTT'AAC Cuts at: 5076 5076 Size: 7486 KasI G'GCGC_C Cuts at: 2366 2366 Size: 7486 KpnI G_GTAC'C Cuts at: 4811 4811 Size: 7486 MfeI C'AATT_G Cuts at: 3750 5085 3750 Size: 1335 6151 MluI A'CGCG_T Cuts at: 7362 7362 Size: 7486 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3007 3217 6600 6784 1305 Size: 1702 210 3383 184 2007 Fragments arranged by size: 3383 2007 1702 210 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3780 10 Size: 54 1425 343 1948 3716 Fragments arranged by size: 3716 1948 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2856 3078 3366 4598 5444 5803 5962 Size: 648 1690 222 288 1232 846 359 159 Cuts at: 5962 518 Size: 2042 Fragments arranged by size: 2042 1690 1232 846 648 359 288 222 159 MspA1I CmG'CkG Cuts at: 1606 2203 4902 5579 6727 6972 1606 Size: 597 2699 677 1148 245 2120 Fragments arranged by size: 2699 2120 1148 677 597 245 NarI GG'CG_CC Cuts at: 2367 2367 Size: 7486 NcoI C'CATG_G Cuts at: 513 1065 3499 513 Size: 552 2434 4500 Fragments arranged by size: 4500 2434 552 NdeI CA'TA_TG Cuts at: 387 3987 4595 387 Size: 3600 608 3278 Fragments arranged by size: 3600 3278 608 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7486 NotI GC'GGCC_GC Cuts at: 2051 4933 2051 Size: 2882 4604 NsiI A_TGCA'T Cuts at: 2640 3715 4548 2640 Size: 1075 833 5578 Fragments arranged by size: 5578 1075 833 NspI r_CATG'y Cuts at: 1132 1229 2738 3153 3195 4844 6389 1132 Size: 97 1509 415 42 1649 1545 2229 Fragments arranged by size: 2229 1649 1545 1509 415 97 42 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7486 PciI A'CATG_T Cuts at: 2734 3149 6385 2734 Size: 415 3236 3835 Fragments arranged by size: 3835 3236 415 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7486 PmeI GTTT'AAAC Cuts at: 4790 4790 Size: 7486 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5555 5587 7068 7100 5555 Size: 32 1481 32 5941 Fragments arranged by size: 5941 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2276 2276 Size: 7486 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7486 PsiI TTA'TAA Cuts at: 2966 4080 5056 2966 Size: 1114 976 5396 Fragments arranged by size: 5396 1114 976 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3145 3177 3145 Size: 32 7454 PstI C_TGCA'G Cuts at: 830 2254 3169 4838 830 Size: 1424 915 1669 3478 Fragments arranged by size: 3478 1669 1424 915 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7486 SalI G'TCGA_C Cuts at: 1936 4828 1936 Size: 2892 4594 SbfI CC_TGCA'GG Cuts at: 4838 4838 Size: 7486 ScaI AGT'ACT Cuts at: 3891 5734 7446 3891 Size: 1843 1712 3931 Fragments arranged by size: 3931 1843 1712 SfcI C'TryA_G Cuts at: 826 1045 2250 2382 2764 3165 3474 4555 4758 Size: 219 1205 132 382 401 309 1081 203 Cuts at: 4758 4834 5969 6650 6841 826 Size: 76 1135 681 191 1471 Fragments arranged by size: 1471 1205 1135 1081 681 401 382 309 219 203 191 132 76 SfoI GGC'GCC Cuts at: 2368 2368 Size: 7486 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7486 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7486 SmaI CCC'GGG Cuts at: 1872 4813 1872 Size: 2941 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4266 4306 5590 6491 6753 820 Size: 197 926 2323 40 1284 901 262 1553 Fragments arranged by size: 2323 1553 1284 926 901 262 197 40 SnaBI TAC'GTA Cuts at: 493 5277 493 Size: 4784 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7486 SphI G_CATG'C Cuts at: 1132 4844 1132 Size: 3712 3774 SspI AAT'ATT Cuts at: 5 52 4116 4323 5410 5 Size: 47 4064 207 1087 2081 Fragments arranged by size: 4064 2081 1087 207 47 StuI AGG'CCT Cuts at: 1517 3681 1517 Size: 2164 5322 StyI C'CwwG_G Cuts at: 513 1065 2830 3499 4925 513 Size: 552 1765 669 1426 3074 Fragments arranged by size: 3074 1765 1426 669 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3856 5513 5530 5683 5868 6313 325 Size: 1255 2276 1657 17 153 185 445 1498 Fragments arranged by size: 2276 1657 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3146 3152 3889 Size: 274 80 33 51 1443 1169 6 737 Cuts at: 3889 4700 5732 7444 96 Size: 811 1032 1712 138 Fragments arranged by size: 1712 1443 1169 1032 811 737 274 138 80 51 33 6 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3852 3884 3898 3930 3942 3974 7446 7478 3852 Size: 32 14 32 12 32 3472 32 3860 Fragments arranged by size: 3860 3472 32 32 32 32 14 12 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7486 XbaI T'CTAG_A Cuts at: 4822 4822 Size: 7486 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3506 3506 Size: 7486 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7486 XmaI C'CCGG_G Cuts at: 1870 4811 1870 Size: 2941 4545 XmnI GAAnn'nnTTC Cuts at: 2493 5615 2493 Size: 3122 4364 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5294 276 Size: 53 83 186 936 3760 2468 Fragments arranged by size: 3760 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI AvrII BaeI BbvCI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI FseI FspAI NaeI NgoMIV NruI PacI PfoI PmlI PspOMI PspXI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI