(Linear) (Six-base) MAPSORT of: KIEE3435.seq Check: 1151 from: 1 to: 2781 KIEE3435.orf With 182 enzymes: SgfI * March 22, 2012 16:34 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 456 1567 2257 2781 Size: 456 1111 690 524 Fragments arranged by size: 1111 690 524 456 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 448 540 1028 2781 Size: 448 92 488 1753 Fragments arranged by size: 1753 488 448 92 AflII C'TTAA_G Cuts at: 0 2301 2781 Size: 2301 480 AflIII A'CryG_T Cuts at: 0 729 1144 2781 Size: 729 415 1637 Fragments arranged by size: 1637 729 415 AgeI A'CCGG_T Cuts at: 0 2396 2781 Size: 2396 385 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1101 2781 Size: 1101 1680 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 321 353 2781 Size: 321 32 2428 Fragments arranged by size: 2428 321 32 AlwNI CAG_nnn'CTG Cuts at: 0 274 448 937 1220 2781 Size: 274 174 489 283 1561 Fragments arranged by size: 1561 489 283 274 174 ApaLI G'TGCA_C Cuts at: 0 278 725 2781 Size: 278 447 2056 Fragments arranged by size: 2056 447 278 ApoI r'AATT_y Cuts at: 0 649 1280 1487 1620 1718 1763 2063 2781 Size: 649 631 207 133 98 45 300 718 Fragments arranged by size: 718 649 631 300 207 133 98 45 AseI AT'TA_AT Cuts at: 0 662 2781 Size: 662 2119 BanI G'GyrC_C Cuts at: 0 361 2781 Size: 361 2420 BbeI G_GCGC'C Cuts at: 0 365 2781 Size: 365 2416 BbsI GAAGACnn'nnnn_ Cuts at: 0 1967 2105 2781 Size: 1967 138 676 Fragments arranged by size: 1967 676 138 BciVI GTATCCnnnnn_n' Cuts at: 0 1895 2781 Size: 1895 886 BclI T'GATC_A Cuts at: 0 1122 1386 2781 Size: 1122 264 1395 Fragments arranged by size: 1395 1122 264 BfrBI ATG'CAT Cuts at: 0 633 1708 2541 2781 Size: 633 1075 833 240 Fragments arranged by size: 1075 833 633 240 BglII A'GATC_T Cuts at: 0 432 2781 Size: 432 2349 Bme1580I G_kGCm'C Cuts at: 0 282 729 2781 Size: 282 447 2052 Fragments arranged by size: 2052 447 282 BmgBI CAC'GTC Cuts at: 0 1363 2781 Size: 1363 1418 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 381 2781 Size: 381 2400 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1526 1865 1874 2150 2781 Size: 1526 339 9 276 631 Fragments arranged by size: 1526 631 339 276 9 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 2246 2781 Size: 2246 535 BsaI GGTCTCn'nnnn_ Cuts at: 0 21 2233 2781 Size: 21 2212 548 Fragments arranged by size: 2212 548 21 BsaBI GATnn'nnATC Cuts at: 0 2153 2781 Size: 2153 628 BsaHI Gr'CG_yC Cuts at: 0 362 2781 Size: 362 2419 BsaWI w'CCGG_w Cuts at: 0 23 1898 2396 2781 Size: 23 1875 498 385 Fragments arranged by size: 1875 498 385 23 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1847 1877 2781 Size: 1847 30 904 Fragments arranged by size: 1847 904 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1661 2781 Size: 1661 1120 BseYI C'CCAG_C Cuts at: 0 107 122 1300 2781 Size: 107 15 1178 1481 Fragments arranged by size: 1481 1178 107 15 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 79 950 1046 2781 Size: 79 871 96 1735 Fragments arranged by size: 1735 871 96 79 BsiEI CG_ry'CG Cuts at: 0 49 2781 Size: 49 2732 BsiHKAI G_wGCw'C Cuts at: 0 282 729 2781 Size: 282 447 2052 Fragments arranged by size: 2052 447 282 BsmI GAATG_Cn' Cuts at: 0 2262 2781 Size: 2262 519 Bsp1286I G_dGCh'C Cuts at: 0 282 729 2781 Size: 282 447 2052 Fragments arranged by size: 2052 447 282 BspHI T'CATG_A Cuts at: 0 1767 2781 Size: 1767 1014 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 456 1567 1908 2257 2326 2781 Size: 456 1111 341 349 69 455 Fragments arranged by size: 1111 456 455 349 341 69 BsrBI CCG'CTC Cuts at: 0 210 228 2781 Size: 210 18 2553 Fragments arranged by size: 2553 210 18 BsrDI GCAATG_nn' Cuts at: 0 1710 2129 2767 2781 Size: 1710 419 638 14 Fragments arranged by size: 1710 638 419 14 BsrFI r'CCGG_y Cuts at: 0 2396 2781 Size: 2396 385 BsrGI T'GTAC_A Cuts at: 0 1147 2695 2781 Size: 1147 1548 86 Fragments arranged by size: 1548 1147 86 BstAPI GCAn_nnn'nTGC Cuts at: 0 406 448 2781 Size: 406 42 2333 Fragments arranged by size: 2333 406 42 BstXI CCAn_nnnn'nTGG Cuts at: 0 584 692 1783 2781 Size: 584 108 1091 998 Fragments arranged by size: 1091 998 584 108 BstYI r'GATC_y Cuts at: 0 432 522 2781 Size: 432 90 2259 Fragments arranged by size: 2259 432 90 Bsu36I CC'TnA_GG Cuts at: 0 203 2781 Size: 203 2578 BtgI C'CryG_G Cuts at: 0 1494 2781 Size: 1494 1287 BtsI GCAGTG_nn' Cuts at: 0 1083 2781 Size: 1083 1698 EaeI y'GGCC_r Cuts at: 0 46 1773 2781 Size: 46 1727 1008 Fragments arranged by size: 1727 1008 46 EagI C'GGCC_G Cuts at: 0 46 2781 Size: 46 2735 EarI CTCTTCn'nnn_ Cuts at: 0 665 780 1527 1817 2184 2781 Size: 665 115 747 290 367 597 Fragments arranged by size: 747 665 597 367 290 115 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 125 161 182 2781 Size: 125 36 21 2599 Fragments arranged by size: 2599 125 36 21 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 448 540 1028 1526 1865 1874 2150 2781 Size: 448 92 488 498 339 9 276 631 Fragments arranged by size: 631 498 488 448 339 276 92 9 EcoO109I rG'GnC_Cy Cuts at: 0 271 2781 Size: 271 2510 EcoRI G'AATT_C Cuts at: 0 1718 2063 2781 Size: 1718 345 718 Fragments arranged by size: 1718 718 345 EcoRV GAT'ATC Cuts at: 0 1971 2781 Size: 1971 810 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 841 2781 Size: 841 1940 HaeII r_GCGC'y Cuts at: 0 365 2781 Size: 365 2416 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1042 1074 1640 1672 2781 Size: 1042 32 566 32 1109 Fragments arranged by size: 1109 1042 566 32 32 KasI G'GCGC_C Cuts at: 0 361 2781 Size: 361 2420 MfeI C'AATT_G Cuts at: 0 1745 2781 Size: 1745 1036 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1002 1212 2781 Size: 1002 210 1569 Fragments arranged by size: 1569 1002 210 MscI TGG'CCA Cuts at: 0 1775 2781 Size: 1775 1006 MslI CAynn'nnrTG Cuts at: 0 851 1073 1361 2593 2781 Size: 851 222 288 1232 188 Fragments arranged by size: 1232 851 288 222 188 MspA1I CmG'CkG Cuts at: 0 198 2781 Size: 198 2583 NarI GG'CG_CC Cuts at: 0 362 2781 Size: 362 2419 NcoI C'CATG_G Cuts at: 0 1494 2781 Size: 1494 1287 NdeI CA'TA_TG Cuts at: 0 1982 2590 2781 Size: 1982 608 191 Fragments arranged by size: 1982 608 191 NotI GC'GGCC_GC Cuts at: 0 46 2781 Size: 46 2735 NsiI A_TGCA'T Cuts at: 0 635 1710 2543 2781 Size: 635 1075 833 238 Fragments arranged by size: 1075 833 635 238 NspI r_CATG'y Cuts at: 0 733 1148 1190 2781 Size: 733 415 42 1591 Fragments arranged by size: 1591 733 415 42 PciI A'CATG_T Cuts at: 0 729 1144 2781 Size: 729 415 1637 Fragments arranged by size: 1637 729 415 PpuMI rG'GwC_Cy Cuts at: 0 271 2781 Size: 271 2510 PsiI TTA'TAA Cuts at: 0 961 2075 2781 Size: 961 1114 706 Fragments arranged by size: 1114 961 706 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 1140 1172 2781 Size: 1140 32 1609 Fragments arranged by size: 1609 1140 32 PstI C_TGCA'G Cuts at: 0 249 1164 2781 Size: 249 915 1617 Fragments arranged by size: 1617 915 249 ScaI AGT'ACT Cuts at: 0 1886 2781 Size: 1886 895 SfcI C'TryA_G Cuts at: 0 245 377 759 1160 1469 2550 2753 2781 Size: 245 132 382 401 309 1081 203 28 Fragments arranged by size: 1081 401 382 309 245 203 132 28 SfoI GGC'GCC Cuts at: 0 363 2781 Size: 363 2418 SmlI C'TyrA_G Cuts at: 0 2261 2301 2781 Size: 2261 40 480 Fragments arranged by size: 2261 480 40 SspI AAT'ATT Cuts at: 0 2111 2318 2781 Size: 2111 207 463 Fragments arranged by size: 2111 463 207 StuI AGG'CCT Cuts at: 0 1676 2781 Size: 1676 1105 StyI C'CwwG_G Cuts at: 0 825 1494 2781 Size: 825 669 1287 Fragments arranged by size: 1287 825 669 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1851 2781 Size: 1851 930 TatI w'GTAC_w Cuts at: 0 1141 1147 1884 2695 2781 Size: 1141 6 737 811 86 Fragments arranged by size: 1141 811 737 86 6 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1847 1879 1893 1925 1937 1969 2781 Size: 1847 32 14 32 12 32 812 Fragments arranged by size: 1847 812 32 32 32 14 12 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1501 2781 Size: 1501 1280 XmnI GAAnn'nnTTC Cuts at: 0 488 2781 Size: 488 2293 Enzymes that do cut: AarI AcuI AflII AflIII AgeI AlfI AloI AlwNI ApaLI ApoI AseI BanI BbeI BbsI BciVI BclI BfrBI BglII Bme1580I BmgBI BplI BpmI BpuEI BsaI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrGI BstAPI BstXI BstYI Bsu36I BtgI BtsI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI HaeII Hin4I KasI MfeI MmeI MscI MslI MspA1I NarI NcoI NdeI NotI NsiI NspI PciI PpuMI PsiI PsrI PstI ScaI SfcI SfoI SmlI SspI StuI StyI TaqII TatI TstI XcmI XmnI Enzymes that do not cut: AatII AccI Acc65I AclI AfeI AhdI AleI ApaI AscI AsiSI AvaI AvrII BaeI BamHI BanII BbvCI BcgI BglI BlpI BmrI BmtI Bpu10I BsaAI BsiWI BsmBI BspEI BssHII BssSI BstBI BstEII BstZ17I BtgZI ClaI CspCI DraI DraIII DrdI EcoICRI EcoNI FseI FspI FspAI HincII HindIII HpaI KpnI MluI NaeI NgoMIV NheI NruI PacI PasI PflMI PfoI PmeI PmlI PpiI PshAI PspOMI PspXI PvuI PvuII RsrII SacI SacII SalI SanDI SapI SbfI SexAI SfiI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SwaI Tth111I XbaI XhoI XmaI ZraI