Restriction map for
pFN21AE3428
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# NO CUT ENZYMES ( 111 enzymes) #
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AatII AccIII AclI AflII AflIII
AgeI Alw44I AocI Aor51HI ApaI
ApaLI AscI AspI AviII AvrII
BalI BamHI BbrPI BcgI BcnI
BfrI BlnI BlpI Bpu1102I BsaAI
BsaBI BsaWI BseAI BsiEI BsiWI
BsmBI BspDI BspEI BspLU11I BspMI
BsrFI BssHII BssSI BstAPI BstEII
BstPI BstXI Bsu36I CelII Cfr10I
ClaI CpoI DraIII EagI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT14I EcoT22I FseI
FspI HgaI HpaI HphI KspI
MamI MluI MluNI MroI MscI
NaeI NciI NcoI NgoMIV NheI
NotI NruI NsiI NspI PacI
PflFI PinAI PmaCI PmeI PmlI
PshAI Psp1406I PstI PvuI RsrII
SacII SalI SbfI ScaI SexAI
SfiI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspI StuI StyI
SwaI Tth111I XbaI XcmI XmaCI
XmaI
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# ONE CUT ENZYMES ( 67 enzymes) #
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Acc65I 1656 GGTACC G^GTACC
AccI 305 GTATAC GT^[AC][GT]AC
AccII 87 CGCG CG^CG
AcyI 128 GGCGCC G[GA]^CG[CT]C
AhdI 856 GACATTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AseI 477 ATTAAT AT^TAAT
AsnI 477 ATTAAT AT^TAAT
Asp700I 548 GAAATGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1656 GGTACC G^GTACC
AspEI 856 GACATTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 980 CTCGAG C^[CT]CG[GA]G
BbeI 128 GGCGCC GGCGC^C
BbvCI 1243 CCTCAGC CCTCAGC(-5/-2)
BciVI 584 GTATCC GTATCC(6/5)
BclI 1575 TGATCA T^GATCA
BglI 1048 GCCTTGTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 866 CTGGAG CTGGAG(16/14)
BsaHI 128 GGCGCC G[GA]^CG[CT]C
BsaI 200 GAGACC GGTCTC(1/5)
BsgI 1028 GTGCAG GTGCAG(16/14)
BsiYI 1738 CCAGACACTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 1738 CCAGACACTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsoBI 980 CTCGAG C^[CT]CG[GA]G
Bsp1407I 1227 TGTACA T^GTACA
BspHI 1578 TCATGA T^CATGA
BsrDI 1603 GCAATG GCAATG(2/0)
BsrGI 1227 TGTACA T^GTACA
BssKI 1544 CCAGG ^CC[ACGT]GG
Bst1107I 305 GTATAC GTA^TAC
BstBI 419 TTCGAA TT^CGAA
BstNI 1544 CCAGG CC^[AT]GG
BstUI 87 CGCG CG^CG
BstZ17I 305 GTATAC GTA^TAC
DrdI 240 GACTGACTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 187 CCACGG C^C[GA][CT]GG
Eam1105I 856 GACATTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRII 1544 CCAGG ^CC[AT]GG
FauI 111 GCGGG CCCGC(4/6)
FbaI 1575 TGATCA T^GATCA
HaeII 128 GGCGCC [GA]GCGC^[CT]
Hin1I 128 GGCGCC G[GA]^CG[CT]C
HindIII 845 AAGCTT A^AGCTT
KasI 128 GGCGCC G^GCGCC
KpnI 1656 GGTACC GGTAC^C
MfeI 557 CAATTG C^AATTG
MunI 557 CAATTG C^AATTG
MvaI 1544 CCAGG CC^[AT]GG
MvnI 87 CGCG CG^CG
NarI 128 GGCGCC GG^CGCC
NdeI 741 CATATG CA^TATG
NspV 419 TTCGAA TT^CGAA
PaeR7I 980 CTCGAG C^TCGAG
PflMI 1738 CCAGACACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 1445 GGGACCT [GA]G^G[AT]CC[CT]
PshBI 477 ATTAAT AT^TAAT
PvuII 1779 CAGCTG CAG^CTG
RcaI 1578 TCATGA T^CATGA
SacI 1061 GAGCTC GAGCT^C
ScrFI 1544 CCAGG CC^[ACGT]GG
SfaNI 961 GCATC GCATC(5/9)
SfoI 128 GGCGCC GGC^GCC
SfuI 419 TTCGAA TT^CGAA
SspBI 1227 TGTACA T^GTACA
Tsp45I 1171 GTCAC ^GT[GC]AC
Van91I 1738 CCAGACACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 980 CTCGAG C^TCGAG
XmnI 548 GAAATGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 25 enzymes) #
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AspHI 869 GAGCAC G[AT]GC[AT]^C
1061 GAGCTC
BanI 128 GGCGCC G^G[CT][GA]CC
1656 GGTACC
BanII 75 GAGCCC G[GA]GC[CT]^C
1061 GAGCTC
BbsI 217 GAAGAC GAAGAC(2/6)
1707 GAAGAC
BfaI 463 CTAG C^TAG
1336 CTAG
BpuAI 217 GAAGAC GAAGAC(2/6)
1707 GAAGAC
BseRI 1712 CTCCTC GAGGAG(10/8)
343 GAGGAG
BsiHKAI 869 GAGCAC G[AT]GC[AT]^C
1061 GAGCTC
BsmFI 1445 GGGAC GGGAC(10/14)
1771 GGGAC
BsmI 385 GAATGC GAATGC(1/-1)
656 GAATGC
BsrBI 62 CCGCTC CCGCTC(-3/-3)
1614 GAGCGG
DraI 967 TTTAAA TTT^AAA
1180 TTTAAA
DraII 630 AGGCCCC [GA]G^G[ACGT]CC[CT]
1445 GGGACCT
EaeI 23 TGGCCG [CT]^GGCC[GA]
1557 TGGCCG
EcoO109I 630 AGGCCCC [GA]G^G[ACGT]CC[CT]
1445 GGGACCT
HapII 72 CCGG C^CGG
108 CCGG
HincII 330 GTCAAC GT[CT]^[GA]AC
1423 GTTGAC
HindII 330 GTCAAC GT[CT]^[GA]AC
1423 GTTGAC
HpaII 72 CCGG C^CGG
108 CCGG
MaeI 463 CTAG C^TAG
1336 CTAG
MaeII 495 ACGT A^CGT
1554 ACGT
MslI 737 CATCCATATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1414 CACTCAAGTG
MspI 72 CCGG C^CGG
108 CCGG
SapI 232 GAAGAGC GCTCTTC(1/4)
619 GAAGAGC
SfcI 280 CTACAG C^T[GA][CT]AG
1159 CTACAG
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# 3 OR MORE CUT ENZYMES ( 55 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AvaII BbvI BglII
BmyI BsaJI BsmAI Bsp1286I BsrI
BstF5I BstYI Cac8I CfoI Cfr13I
DdeI DpnII EarI Fnu4HI FokI
HaeIII HhaI HinP1I HinfI ItaI
Ksp632I MaeIII MboI MboII MflI
MnlI MseI MspA1I MwoI NdeII
NlaIII NlaIV PleI RsaI Sau3AI
Sau96I SmlI TaqI TfiI Tru9I
TseI Tsp509I TspRI TthHB8I XhoII