(Circular) (Six-base) MAPSORT of: pFN21AE3428.seq Check: 6866 from: 1 to: 6511 pFN21AE3428 6511 bp With 182 enzymes: SgfI * March 28, 2012 12:44 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4321 278 Size: 53 83 186 936 2785 2468 Fragments arranged by size: 2785 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2311 3854 1937 Size: 374 1543 4594 Fragments arranged by size: 4594 1543 374 Acc65I G'GTAC_C Cuts at: 3661 3832 3661 Size: 171 6340 AclI AA'CG_TT Cuts at: 1563 4638 5011 1563 Size: 3075 373 3063 Fragments arranged by size: 3075 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2603 2754 3318 3534 3731 4574 5958 6226 Size: 971 151 564 216 197 843 1384 268 Cuts at: 6226 1632 Size: 1917 Fragments arranged by size: 1917 1384 971 843 564 268 216 197 151 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6314 AflIII A'CryG_T Cuts at: 1203 5410 6387 1203 Size: 4207 977 1327 Fragments arranged by size: 4207 1327 977 AgeI A'CCGG_T Cuts at: 5342 5342 Size: 6511 AhdI GACnn_n'nnGTC Cuts at: 2866 5240 2866 Size: 2374 4137 AleI CACnn'nnGTG Cuts at: 1166 3423 1166 Size: 2257 4254 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6511 AlwNI CAG_nnn'CTG Cuts at: 2777 3749 3779 4254 5826 2777 Size: 972 30 475 1572 3462 Fragments arranged by size: 3462 1572 972 475 30 ApaLI G'TGCA_C Cuts at: 4568 5724 4568 Size: 1156 5355 ApoI r'AATT_y Cuts at: 1493 2767 3125 3243 3651 3820 4046 4198 6307 Size: 1274 358 118 408 169 226 152 2109 Cuts at: 6307 1493 Size: 1697 Fragments arranged by size: 2109 1697 1274 408 358 226 169 152 118 AseI AT'TA_AT Cuts at: 160 1032 2483 5065 160 Size: 872 1451 2582 1606 Fragments arranged by size: 2582 1606 1451 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6511 AvaI C'yCGr_G Cuts at: 1870 1943 2985 3836 1870 Size: 73 1042 851 4545 Fragments arranged by size: 4545 1042 851 73 BamHI G'GATC_C Cuts at: 3841 4208 3841 Size: 367 6144 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2133 3661 3832 5287 618 Size: 325 217 627 346 1528 171 1455 1842 Fragments arranged by size: 1842 1528 1455 627 346 325 217 171 BanII G_rGCy'C Cuts at: 1391 2084 3070 3830 1391 Size: 693 986 760 4072 Fragments arranged by size: 4072 986 760 693 BbeI G_GCGC'C Cuts at: 2137 2137 Size: 6511 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2229 3719 928 Size: 971 330 1490 3720 Fragments arranged by size: 3720 1490 971 330 BbvCI CC'TCA_GC Cuts at: 3249 3249 Size: 6511 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4702 4736 1903 Size: 34 2765 34 3678 Fragments arranged by size: 3678 2765 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2600 4403 5613 2600 Size: 1803 1210 3498 Fragments arranged by size: 3498 1803 1210 BclI T'GATC_A Cuts at: 1548 3580 1548 Size: 2032 4479 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3059 5122 136 Size: 107 122 71 631 605 1387 2063 1525 Fragments arranged by size: 2063 1525 1387 631 605 122 107 71 BglII A'GATC_T Cuts at: 2434 2701 3698 6506 2434 Size: 267 997 2808 2439 Fragments arranged by size: 2808 2439 997 267 BlpI GC'TnA_GC Cuts at: 3928 3928 Size: 6511 Bme1580I G_kGCm'C Cuts at: 1790 4572 5728 1790 Size: 2782 1156 2573 Fragments arranged by size: 2782 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6511 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3759 4242 5200 Size: 416 53 83 399 24 2342 483 958 Cuts at: 5200 442 Size: 1753 Fragments arranged by size: 2342 1753 958 483 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6511 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2892 5171 1239 Size: 1653 2279 2579 Fragments arranged by size: 2579 2279 1653 Bpu10I CC'TnA_GC Cuts at: 2475 3249 2475 Size: 774 5737 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2526 3054 3406 4636 5501 5799 2526 Size: 528 352 1230 865 298 3238 Fragments arranged by size: 3238 1230 865 528 352 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2199 5174 882 Size: 617 700 2975 2219 Fragments arranged by size: 2975 2219 700 617 BsaAI yAC'GTr Cuts at: 493 1204 4302 493 Size: 711 3098 2702 Fragments arranged by size: 3098 2702 711 BsaBI GATnn'nnATC Cuts at: 6505 6505 Size: 6511 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2134 4318 4700 5335 Size: 53 83 186 936 601 2184 382 635 Cuts at: 5335 275 Size: 1451 Fragments arranged by size: 2184 1451 936 635 601 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4944 5342 5616 5763 6174 4944 Size: 398 274 147 411 5281 Fragments arranged by size: 5281 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6286 Fragments arranged by size: 6286 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2363 3708 1186 Size: 1177 1345 3989 Fragments arranged by size: 3989 1345 1177 BseYI C'CCAG_C Cuts at: 2777 5714 6210 2777 Size: 2937 496 3078 Fragments arranged by size: 3078 2937 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3054 1163 Size: 573 1318 4620 Fragments arranged by size: 4620 1318 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3961 4722 5750 1540 Size: 275 186 1960 761 1028 2301 Fragments arranged by size: 2301 1960 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2878 3070 4572 4657 5728 2878 Size: 192 1502 85 1071 3661 Fragments arranged by size: 3661 1502 1071 192 85 BsmI GAATG_Cn' Cuts at: 2396 2667 4022 4115 2396 Size: 271 1355 93 4792 Fragments arranged by size: 4792 1355 271 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2084 2878 3070 3830 4572 4657 5728 Size: 399 294 794 192 760 742 85 1071 Cuts at: 5728 1391 Size: 2174 Fragments arranged by size: 2174 1071 794 760 742 399 294 192 85 BspEI T'CCGG_A Cuts at: 6174 6174 Size: 6511 BspHI T'CATG_A Cuts at: 3583 4400 6130 3583 Size: 817 1730 3964 Fragments arranged by size: 3964 1730 817 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3866 844 Size: 3022 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2069 3621 4398 1394 Size: 429 246 1552 777 3507 Fragments arranged by size: 3507 1552 777 429 246 BsrDI GCAATG_nn' Cuts at: 65 1248 3615 5006 5180 65 Size: 1183 2367 1391 174 1396 Fragments arranged by size: 2367 1396 1391 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5155 5342 1700 Size: 110 3345 187 2869 Fragments arranged by size: 3345 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3232 96 Size: 3136 3375 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6511 BssSI C'ACGA_G Cuts at: 4571 5583 4571 Size: 1012 5499 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6511 BstBI TT'CG_AA Cuts at: 2425 5315 2425 Size: 2890 3621 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6511 BstYI r'GATC_y Cuts at: 1972 2434 2701 3698 3841 4208 4593 4610 6062 Size: 462 267 997 143 367 385 17 1452 Cuts at: 6062 6148 6160 6506 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 997 462 385 367 346 267 143 86 17 12 BstZ17I GTA'TAC Cuts at: 2312 2312 Size: 6511 BtgI C'CryG_G Cuts at: 513 1065 2004 2192 513 Size: 552 939 188 4832 Fragments arranged by size: 4832 939 552 188 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5772 Fragments arranged by size: 5772 683 56 BtsI GCAGTG_nn' Cuts at: 3042 4027 4821 4841 3042 Size: 985 794 20 4712 Fragments arranged by size: 4712 985 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6511 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6476 DraI TTT'AAA Cuts at: 2974 3187 3815 2974 Size: 213 628 5670 Fragments arranged by size: 5670 628 213 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2251 5518 809 Size: 507 935 3267 1802 Fragments arranged by size: 3267 1802 935 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2028 3562 3958 Size: 54 1425 50 275 18 198 1534 396 Cuts at: 3958 4847 6476 8 Size: 889 1629 43 Fragments arranged by size: 1629 1534 1425 889 396 275 198 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3958 1537 Size: 275 2146 4090 Fragments arranged by size: 4090 2146 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2011 2098 2197 2232 2619 2730 3337 4441 Size: 658 87 99 35 387 111 607 1104 Cuts at: 4441 1353 Size: 3423 Fragments arranged by size: 3423 1104 658 607 387 111 99 87 35 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2111 5098 5472 5618 387 Size: 1064 138 522 2987 374 146 1280 Fragments arranged by size: 2987 1280 1064 522 374 146 138 EcoICRI GAG'CTC Cuts at: 3068 3068 Size: 6511 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2603 2754 2892 3318 3534 3731 4574 Size: 393 971 151 138 426 216 197 843 Cuts at: 4574 5171 5958 6226 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 971 843 787 597 426 393 268 216 197 151 138 EcoO109I rG'GnC_Cy Cuts at: 2636 3451 2636 Size: 815 5696 EcoRI G'AATT_C Cuts at: 3820 4198 3820 Size: 378 6133 FspI TGC'GCA Cuts at: 5017 5017 Size: 6511 HaeII r_GCGC'y Cuts at: 1678 2137 5658 1678 Size: 459 3521 2531 Fragments arranged by size: 3521 2531 459 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2283 2315 2339 2342 2371 Size: 32 135 32 711 32 24 3 29 Cuts at: 2371 2374 2485 2517 2852 2884 3339 3371 3485 Size: 3 111 32 335 32 455 32 114 Cuts at: 3485 3517 5152 5184 5226 5258 1373 Size: 32 1635 32 42 32 2626 Fragments arranged by size: 2626 1635 711 455 335 135 114 111 42 32 32 32 32 32 32 32 32 32 29 24 3 3 HincII GTy'rAC Cuts at: 669 1657 1938 2337 3430 3855 4101 669 Size: 988 281 399 1093 425 246 3079 Fragments arranged by size: 3079 1093 988 425 399 281 246 HindIII A'AGCT_T Cuts at: 748 2850 748 Size: 2102 4409 HpaI GTT'AAC Cuts at: 4101 4101 Size: 6511 KasI G'GCGC_C Cuts at: 2133 2133 Size: 6511 KpnI G_GTAC'C Cuts at: 3665 3836 3665 Size: 171 6340 MfeI C'AATT_G Cuts at: 2562 4110 2562 Size: 1548 4963 MluI A'CGCG_T Cuts at: 6387 6387 Size: 6511 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2397 3282 5625 5809 1305 Size: 1092 885 2343 184 2007 Fragments arranged by size: 2343 2007 1092 885 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4689 Fragments arranged by size: 4689 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2746 3423 4469 4828 4987 518 Size: 648 1580 677 1046 359 159 2042 Fragments arranged by size: 2042 1580 1046 677 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2033 2045 3786 3927 4604 5752 5997 1606 Size: 427 12 1741 141 677 1148 245 2120 Fragments arranged by size: 2120 1741 1148 677 427 245 141 12 NarI GG'CG_CC Cuts at: 2134 2134 Size: 6511 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5020 Fragments arranged by size: 5020 939 552 NdeI CA'TA_TG Cuts at: 387 2747 387 Size: 2360 4151 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6511 NotI GC'GGCC_GC Cuts at: 3958 3958 Size: 6511 NspI r_CATG'y Cuts at: 1132 1229 3869 5414 1132 Size: 97 2640 1545 2229 Fragments arranged by size: 2640 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6511 PciI A'CATG_T Cuts at: 5410 5410 Size: 6511 PflMI CCAn_nnn'nTGG Cuts at: 1108 3749 1108 Size: 2641 3870 PmeI GTTT'AAAC Cuts at: 3815 3815 Size: 6511 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4580 4612 6093 6125 4580 Size: 32 1481 32 4966 Fragments arranged by size: 4966 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3451 3451 Size: 6511 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6511 PsiI TTA'TAA Cuts at: 4081 4081 Size: 6511 PstI C_TGCA'G Cuts at: 830 3863 830 Size: 3033 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6511 PvuII CAG'CTG Cuts at: 3786 3786 Size: 6511 SacI G_AGCT'C Cuts at: 3070 3070 Size: 6511 SalI G'TCGA_C Cuts at: 1936 3853 1936 Size: 1917 4594 SapI GCTCTTCn'nnn_ Cuts at: 2232 2619 2232 Size: 387 6124 SbfI CC_TGCA'GG Cuts at: 3863 3863 Size: 6511 ScaI AGT'ACT Cuts at: 4759 6471 4759 Size: 1712 4799 SfcI C'TryA_G Cuts at: 826 1045 2285 3164 3859 4994 5675 5866 826 Size: 219 1240 879 695 1135 681 191 1471 Fragments arranged by size: 1471 1240 1135 879 695 681 219 191 SfoI GGC'GCC Cuts at: 2135 2135 Size: 6511 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6511 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6511 SmaI CCC'GGG Cuts at: 1872 3838 1872 Size: 1966 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2541 2985 3069 3421 4615 5516 Size: 197 926 598 444 84 352 1194 901 Cuts at: 5516 5778 820 Size: 262 1553 Fragments arranged by size: 1553 1194 926 901 598 444 352 262 197 84 SnaBI TAC'GTA Cuts at: 493 4302 493 Size: 3809 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6511 SphI G_CATG'C Cuts at: 1132 3869 1132 Size: 2737 3774 SspI AAT'ATT Cuts at: 5 52 4435 5 Size: 47 4383 2081 Fragments arranged by size: 4383 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6511 StyI C'CwwG_G Cuts at: 513 1065 2004 3950 513 Size: 552 939 1946 3074 Fragments arranged by size: 3074 1946 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4538 4555 4708 4893 5338 325 Size: 1255 2958 17 153 185 445 1498 Fragments arranged by size: 2958 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2517 3232 4757 Size: 274 80 33 51 1443 540 715 1525 Cuts at: 4757 6469 96 Size: 1712 138 Fragments arranged by size: 1712 1525 1443 715 540 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3544 3576 6471 6503 3544 Size: 32 2895 32 3552 Fragments arranged by size: 3552 2895 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6511 XbaI T'CTAG_A Cuts at: 3847 3847 Size: 6511 XhoI C'TCGA_G Cuts at: 1943 2985 1943 Size: 1042 5469 XmaI C'CCGG_G Cuts at: 1870 3836 1870 Size: 1966 4545 XmnI GAAnn'nnTTC Cuts at: 2557 4640 2557 Size: 2083 4428 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4319 276 Size: 53 83 186 936 2785 2468 Fragments arranged by size: 2785 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI BstXI Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SrfI SwaI XcmI