(Linear) (Six-base) MAPSORT of: KIEE3428.seq Check: 3209 from: 1 to: 1806 KIEE3428.orf With 182 enzymes: SgfI * March 22, 2012 16:33 .. AccI GT'mk_AC Cuts at: 0 306 1806 Size: 306 1500 Acc65I G'GTAC_C Cuts at: 0 1656 1806 Size: 1656 150 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 598 749 1313 1529 1726 1806 Size: 598 151 564 216 197 80 Fragments arranged by size: 598 564 216 197 151 80 AhdI GACnn_n'nnGTC Cuts at: 0 861 1806 Size: 861 945 AleI CACnn'nnGTG Cuts at: 0 1418 1806 Size: 1418 388 AlwNI CAG_nnn'CTG Cuts at: 0 772 1744 1774 1806 Size: 772 972 30 32 Fragments arranged by size: 972 772 32 30 ApoI r'AATT_y Cuts at: 0 762 1120 1238 1646 1806 Size: 762 358 118 408 160 Fragments arranged by size: 762 408 358 160 118 AseI AT'TA_AT Cuts at: 0 478 1806 Size: 478 1328 AvaI C'yCGr_G Cuts at: 0 980 1806 Size: 980 826 BanI G'GyrC_C Cuts at: 0 128 1656 1806 Size: 128 1528 150 Fragments arranged by size: 1528 150 128 BanII G_rGCy'C Cuts at: 0 79 1065 1806 Size: 79 986 741 Fragments arranged by size: 986 741 79 BbeI G_GCGC'C Cuts at: 0 132 1806 Size: 132 1674 BbsI GAAGACnn'nnnn_ Cuts at: 0 224 1714 1806 Size: 224 1490 92 Fragments arranged by size: 1490 224 92 BbvCI CC'TCA_GC Cuts at: 0 1244 1806 Size: 1244 562 BciVI GTATCCnnnnn_n' Cuts at: 0 595 1806 Size: 595 1211 BclI T'GATC_A Cuts at: 0 1575 1806 Size: 1575 231 BglI GCCn_nnn'nGGC Cuts at: 0 1054 1806 Size: 1054 752 BglII A'GATC_T Cuts at: 0 429 696 1693 1806 Size: 429 267 997 113 Fragments arranged by size: 997 429 267 113 BmrI ACTGGGnnnn_n' Cuts at: 0 1754 1806 Size: 1754 52 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 887 1806 Size: 887 919 Bpu10I CC'TnA_GC Cuts at: 0 470 1244 1806 Size: 470 774 562 Fragments arranged by size: 774 562 470 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 521 1049 1401 1806 Size: 521 528 352 405 Fragments arranged by size: 528 521 405 352 BsaI GGTCTCn'nnnn_ Cuts at: 0 194 1806 Size: 194 1612 BsaHI Gr'CG_yC Cuts at: 0 129 1806 Size: 129 1677 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 358 1703 1806 Size: 358 1345 103 Fragments arranged by size: 1345 358 103 BseYI C'CCAG_C Cuts at: 0 772 1806 Size: 772 1034 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1049 1806 Size: 1049 757 BsiHKAI G_wGCw'C Cuts at: 0 873 1065 1806 Size: 873 192 741 Fragments arranged by size: 873 741 192 BsmI GAATG_Cn' Cuts at: 0 391 662 1806 Size: 391 271 1144 Fragments arranged by size: 1144 391 271 Bsp1286I G_dGCh'C Cuts at: 0 79 873 1065 1806 Size: 79 794 192 741 Fragments arranged by size: 794 741 192 79 BspHI T'CATG_A Cuts at: 0 1578 1806 Size: 1578 228 BsrBI CCG'CTC Cuts at: 0 64 1616 1806 Size: 64 1552 190 Fragments arranged by size: 1552 190 64 BsrDI GCAATG_nn' Cuts at: 0 1610 1806 Size: 1610 196 BsrGI T'GTAC_A Cuts at: 0 1227 1806 Size: 1227 579 BstBI TT'CG_AA Cuts at: 0 420 1806 Size: 420 1386 BstYI r'GATC_y Cuts at: 0 429 696 1693 1806 Size: 429 267 997 113 Fragments arranged by size: 997 429 267 113 BstZ17I GTA'TAC Cuts at: 0 307 1806 Size: 307 1499 BtgI C'CryG_G Cuts at: 0 187 1806 Size: 187 1619 BtsI GCAGTG_nn' Cuts at: 0 1037 1806 Size: 1037 769 DraI TTT'AAA Cuts at: 0 969 1182 1806 Size: 969 213 624 Fragments arranged by size: 969 624 213 DrdI GACnn_nn'nnGTC Cuts at: 0 246 1806 Size: 246 1560 EaeI y'GGCC_r Cuts at: 0 23 1557 1806 Size: 23 1534 249 Fragments arranged by size: 1534 249 23 EarI CTCTTCn'nnn_ Cuts at: 0 6 93 192 227 614 725 1332 1806 Size: 6 87 99 35 387 111 607 474 Fragments arranged by size: 607 474 387 111 99 87 35 6 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 106 1806 Size: 106 1700 EcoICRI GAG'CTC Cuts at: 0 1063 1806 Size: 1063 743 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 598 749 887 1313 1529 1726 1806 Size: 598 151 138 426 216 197 80 Fragments arranged by size: 598 426 216 197 151 138 80 EcoO109I rG'GnC_Cy Cuts at: 0 631 1446 1806 Size: 631 815 360 Fragments arranged by size: 815 631 360 HaeII r_GCGC'y Cuts at: 0 132 1806 Size: 132 1674 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 278 310 334 337 366 369 480 512 Size: 278 32 24 3 29 3 111 32 Cuts at: 512 847 879 1334 1366 1480 1512 1806 Size: 335 32 455 32 114 32 294 Fragments arranged by size: 455 335 294 278 114 111 32 32 32 32 32 29 24 3 3 HincII GTy'rAC Cuts at: 0 332 1425 1806 Size: 332 1093 381 Fragments arranged by size: 1093 381 332 HindIII A'AGCT_T Cuts at: 0 845 1806 Size: 845 961 KasI G'GCGC_C Cuts at: 0 128 1806 Size: 128 1678 KpnI G_GTAC'C Cuts at: 0 1660 1806 Size: 1660 146 MfeI C'AATT_G Cuts at: 0 557 1806 Size: 557 1249 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 392 1277 1806 Size: 392 885 529 Fragments arranged by size: 885 529 392 MslI CAynn'nnrTG Cuts at: 0 741 1418 1806 Size: 741 677 388 Fragments arranged by size: 741 677 388 MspA1I CmG'CkG Cuts at: 0 28 40 1781 1806 Size: 28 12 1741 25 Fragments arranged by size: 1741 28 25 12 NarI GG'CG_CC Cuts at: 0 129 1806 Size: 129 1677 NdeI CA'TA_TG Cuts at: 0 742 1806 Size: 742 1064 PflMI CCAn_nnn'nTGG Cuts at: 0 1744 1806 Size: 1744 62 PpuMI rG'GwC_Cy Cuts at: 0 1446 1806 Size: 1446 360 PvuII CAG'CTG Cuts at: 0 1781 1806 Size: 1781 25 SacI G_AGCT'C Cuts at: 0 1065 1806 Size: 1065 741 SapI GCTCTTCn'nnn_ Cuts at: 0 227 614 1806 Size: 227 387 1192 Fragments arranged by size: 1192 387 227 SfcI C'TryA_G Cuts at: 0 280 1159 1806 Size: 280 879 647 Fragments arranged by size: 879 647 280 SfoI GGC'GCC Cuts at: 0 130 1806 Size: 130 1676 SmlI C'TyrA_G Cuts at: 0 536 980 1064 1416 1806 Size: 536 444 84 352 390 Fragments arranged by size: 536 444 390 352 84 TatI w'GTAC_w Cuts at: 0 512 1227 1806 Size: 512 715 579 Fragments arranged by size: 715 579 512 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1539 1571 1806 Size: 1539 32 235 Fragments arranged by size: 1539 235 32 XhoI C'TCGA_G Cuts at: 0 980 1806 Size: 980 826 XmnI GAAnn'nnTTC Cuts at: 0 552 1806 Size: 552 1254 Enzymes that do cut: AccI Acc65I AcuI AhdI AleI AlwNI ApoI AseI AvaI BanI BanII BbeI BbsI BbvCI BciVI BclI BglI BglII BmrI BpmI Bpu10I BpuEI BsaI BsaHI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspHI BsrBI BsrDI BsrGI BstBI BstYI BstZ17I BtgI BtsI DraI DrdI EaeI EarI EciI EcoICRI Eco57MI EcoO109I HaeII Hin4I HincII HindIII KasI KpnI MfeI MmeI MslI MspA1I NarI NdeI PflMI PpuMI PvuII SacI SapI SfcI SfoI SmlI TatI TstI XhoI XmnI Enzymes that do not cut: AarI AatII AclI AfeI AflII AflIII AgeI AlfI AloI ApaI ApaLI AscI AsiSI AvrII BaeI BamHI BcgI BfrBI BlpI Bme1580I BmgBI BmtI BplI BsaAI BsaBI BsaWI BsaXI BsiEI BsiWI BsmBI BspEI BspMI BsrFI BssHII BssSI BstAPI BstEII BstXI Bsu36I BtgZI ClaI CspCI DraIII EagI EcoNI EcoRI EcoRV FalI FseI FspI FspAI HpaI MluI MscI NaeI NcoI NgoMIV NheI NotI NruI NsiI NspI PacI PasI PciI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PstI PvuI RsrII SacII SalI SanDI SbfI ScaI SexAI SfiI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI StyI SwaI TaqII Tth111I XbaI XcmI XmaI ZraI