Restriction map for
pFN21AE3408
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# NO CUT ENZYMES ( 189 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI AlwI AocI Aor51HI ApaI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvaI AviII AvrII
BamHI BanI BanII BbeI BbrPI
BbsI BbvCI BbvI BcgI BciVI
BclI BcnI BfrI BglI BglII
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaBI BsaHI BsaI BsaWI BseAI
BsiEI BsiWI BsmAI BsmBI BsmI
BsoBI Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstXI
BstYI BstZ17I Bsu36I CelII CfoI
Cfr10I ClaI CpoI DpnII DraII
DrdI DsaI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRI EcoRV FauI FbaI
Fnu4HI FseI FspI HaeII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HpaI HpaII ItaI KasI
KpnI KspI MaeII MamI MboI
MfeI MflI MluI MroI MspI
MunI MvnI MwoI NaeI NarI
NciI NcoI NdeI NdeII NgoMIV
NheI NotI NruI NspV PacI
PaeR7I PflFI PflMI PinAI PleI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI PvuII
RcaI RsrII SacI SacII SalI
SapI Sau3AI SbfI ScaI SfaNI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI Sse8387I SspBI
StuI SwaI TaqI TseI Tth111I
TthHB8I Van91I XbaI XcmI XhoI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AciI 75 GCGG CCGC(-3/-1)
AfaI 656 GTAC GT^AC
AluI 864 AGCT AG^CT
Alw44I 680 GTGCAC G^TGCAC
AlwNI 55 CAGTGCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 680 GTGCAC G^TGCAC
AspHI 680 GTGCAC G[AT]GC[AT]^C
BalI 26 TGGCCA TGG^CCA
BmyI 680 GTGCAC G[AGT]GC[ACT]^C
BsaJI 282 CCAAGG C^C[ACGT][ACGT]GG
BseRI 46 GAGGAG GAGGAG(10/8)
BsgI 68 CTGCAC GTGCAG(16/14)
BsiHKAI 680 GTGCAC G[AT]GC[AT]^C
BsiYI 530 CCTTTAACTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 530 CCTTTAACTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
Bsp1286I 680 GTGCAC G[AGT]GC[ACT]^C
Cac8I 452 GCATGC GC[ACGT]^[ACGT]GC
DraI 700 TTTAAA TTT^AAA
DraIII 683 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 26 TGGCCA [CT]^GGCC[GA]
EcoT14I 282 CCAAGG C^C[AT][AT]GG
EcoT22I 364 ATGCAT ATGCA^T
HaeIII 27 GGCC GG^CC
HindIII 863 AAGCTT A^AGCTT
HinfI 181 GATTC G^A[ACGT]TC
HphI 618 TCACC GGTGA(8/7)
MluNI 26 TGGCCA TGG^CCA
MscI 26 TGGCCA TGG^CCA
MslI 839 CATACAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 73 CAGCGG C[AC]G^C[GT]G
NsiI 364 ATGCAT ATGCA^T
RsaI 656 GTAC GT^AC
SexAI 116 ACCTGGT A^CC[AT]GGT
SfcI 108 CTACAG C^T[GA][CT]AG
SphI 452 GCATGC GCATG^C
SspI 333 AATATT AAT^ATT
StyI 282 CCAAGG C^C[AT][AT]GG
TfiI 181 GATTC G^A[AT]TC
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# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AvaII 4 GGACC G^G[AT]CC
539 GGTCC
BssKI 60 CCTGG ^CC[ACGT]GG
117 CCTGG
BstF5I 21 GGATG GGATG(2/0)
264 GGATG
BstNI 60 CCTGG CC^[AT]GG
117 CCTGG
Cfr13I 4 GGACC G^G[ACGT]CC
539 GGTCC
DdeI 202 CTGAG C^T[ACGT]AG
560 CTAAG
EarI 514 GAAGAG CTCTTC(1/4)
745 GAAGAG
EcoRII 60 CCTGG ^CC[AT]GG
117 CCTGG
FokI 21 GGATG GGATG(9/13)
264 GGATG
Ksp632I 514 GAAGAG CTCTTC(1/4)
745 GAAGAG
MaeIII 196 GTGAC ^GT[ACGT]AC
437 GTGAC
MboII 514 GAAGA GAAGA(8/7)
745 GAAGA
MvaI 60 CCTGG CC^[AT]GG
117 CCTGG
NlaIV 3 GGGACC GG[ACGT]^[ACGT]CC
834 GGAACC
NspI 90 GCATGT [GA]CATG^[CT]
452 GCATGC
Sau96I 4 GGACC G^G[ACGT]CC
539 GGTCC
ScrFI 60 CCTGG CC^[ACGT]GG
117 CCTGG
Tsp45I 196 GTGAC ^GT[GC]AC
437 GTGAC
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# 3 OR MORE CUT ENZYMES ( 13 enzymes) #
#########################################
AcsI ApoI BfaI BpmI BsmFI
BsrI MaeI MnlI MseI NlaIII
Tru9I Tsp509I TspRI